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Tuberkuloz Ve Toraks 2015The aim of the study was to investigate the storage mite sensitivity and related factors in patients with asthma or asthma and rhinitis.
INTRODUCTION
The aim of the study was to investigate the storage mite sensitivity and related factors in patients with asthma or asthma and rhinitis.
PATIENTS AND METHODS
149 patients with asthma or asthma and rhinitis were included to the study. Prick test was performed after addition of Acarus siro (A. siro), Lepidoglyphus destructor (L. destructor), Glycophagus domesticus (G. domesticus) and Tyrophagus putrescentiae (T. putrescentiae) to the standart prick test. Living conditions, smoking history, allergic diseases status, seasonal variations in symptoms were evaluated by a questionnaire. Besides, respiratory screening spesific IgE, L. destructor spesific IgE was examined in a group of patients who were allergic to storage mites according to prick tests.
RESULT
Prick test results showed that; 115 of the patients were sensitized while 34 of them were not. House dust mite sensitivity was detected as mite 58.3%.The storage mite sensitivity for at least one of the studied species was detected in 61.7% of patients. The sensitivity rates were 50.4%, 48.7%, 47%, %40 for A. siro, L. destructor, G. domesticus and T. putrescentiae, respectively. The storage mite sensivity was found higher in the patients from the rural areas (p< 0.05). L. destructor IgE positiveness was detected in 9.1% of the group that antibody levels were examined. Positive reaction was detected for at least one of the storage mite species in %22.7 of the patients who were considered as not sensitized according to the results of the standart prick tests.
CONCLUSIONS
As a result, storage mites are important allergens in subjects who live in rural areas and close contact with barn, haymow, bin and pantry. Addition of storage mite allergens to the standart prick test panel of patients living in rural area is suitable.
Topics: Acaridae; Adult; Allergens; Animals; Asthma; Female; Humans; Male; Mass Screening; Middle Aged; Mites; Respiratory Hypersensitivity; Rhinitis; Skin Tests
PubMed: 26963305
DOI: 10.5578/tt.8854 -
Turkiye Parazitolojii Dergisi Jun 2015We aimed to determine the species of the house dust mites seen in Erzincan, the number of mites per gram of dust in the houses, and the relationship between temperature...
OBJECTIVE
We aimed to determine the species of the house dust mites seen in Erzincan, the number of mites per gram of dust in the houses, and the relationship between temperature and the number of mite specimens.
METHODS
For this purpose, 54 dust samples collected from 18 houses located in different districts of Erzincan province between November 2013 and January 2014. These samples were examined by a lactic acid precipitation method.
RESULTS
Of the houses in which the dust samples were collected, 94.44% were found to be positive in terms of mites. A total of 844 mite specimens were isolated from the dust samples, and the mean number of mites per gram of dust was found to be 18.34. The most common species was found to be Acarus siro (55.55%) and was followed by Dermatophagoides pteronyssinus (50.00%), Tyrophagus putrescentiae (22.22%), Histiostoma sp. (22.22%), Lepidoglyphus destructor (16.66%), T. perniciosus(11.11%), Euroglyphus maynei (11.11%), Glycyphagus privatus (11.11%), Cheyletus sp. (11.11%), Tarsonemus sp. (11.11%), and Tetranychus sp. (11.11%).
CONCLUSION
Mite-holding rate of the houses in Erzincan province was found to be 94.44%. The mean number of mites per gram of dust was found to be 18.34. The most common mite species was A. siro, which was followed by D. pteronyssinus.
Topics: Animals; Dermatophagoides pteronyssinus; Dust; Female; Housing; Humans; Male; Pyroglyphidae; Temperature; Turkey
PubMed: 26081886
DOI: 10.5152/tpd.2015.3759 -
BMC Microbiology Oct 2022The contribution of the microbiome to pesticide breakdown in agricultural pests remains unclear. We analyzed the effect of pirimiphos-methyl (PM) on four geographically...
BACKGROUND
The contribution of the microbiome to pesticide breakdown in agricultural pests remains unclear. We analyzed the effect of pirimiphos-methyl (PM) on four geographically different cultures of the stored product pest mite Acarus siro (6 L, 6Tu, 6Tk and 6Z) under laboratory experiments. The effect of PM on mite mortality in the impregnated filter paper test was compared.
RESULTS
The mite sensitivity to PM decreased in the order of 6 L, 6Tu, 6Tk, and 6Z. Then, the mites were cultured on PM residues (0.0125 and 1.25 µg·g), and population growth was compared to the control after 21 days of exposure. The comparison showed two situations: (i) increasing population growth for the most sensitive cultures (6 L and 6Tu), and (ii) no effect on mite population growth for tolerant cultures (6Z and 6Tk). The microbiome of mites was analyzed by quantification of 16S DNA copies based on quantitative polymerase chain reaction (qPCR) and by barcode sequencing of the V4 fragment of 16S DNA on samples of 30 individuals from the control and PM residues. The microbiome comprised primarily Solitalea-like organisms in all cultures, except for 6Z, followed by Bacillus, Staphylococcus, and Lactobacillus. The microbiomes of mite cultures did not change with increasing population density. The microbiome of cultures without any differences in population density showed differences in the microbiome composition. A Sodalis-like symbiont replaced Solitalea in the 1.25 µg·g PM in the 6Tk culture. Sodalis and Bacillus prevailed in the microbiomes of PM-treated mites of 6Z culture, while Solitalea was almost absent.
CONCLUSION
The results showed that the microbiome of A. siro differs in composition and in response to PM residues in the diet. The results indicate that Sodalis-like symbionts can help recover mites from pesticide-induced stress.
Topics: Humans; Animals; Acaridae; Pesticide Residues; Mites; Microbiota; Bacteroidetes
PubMed: 36261789
DOI: 10.1186/s12866-022-02661-4 -
PloS One 2023The poultry red mite, Dermanyssus gallinae (Mesostigmata: Dermanyssidae), is a major pest that causes great damage to chicken egg production. In one of our previous...
The poultry red mite, Dermanyssus gallinae (Mesostigmata: Dermanyssidae), is a major pest that causes great damage to chicken egg production. In one of our previous studies, the management of red mites using entomopathogenic fungi was evaluated, and the acaricidal fungus Beauveria bassiana JEF-410 was selected for further research. In this study, we tried to elucidate the pathogenesis of B. bassiana JEF-410 and the defense mechanisms of red mites at a transcriptome level. Red mites collected from a chicken farm were treated with B. bassiana JEF-410. When the mortality of infected red mites reached 50%, transcriptome analyses were performed to determine the interaction between B. bassiana JEF-410 and red mites. Uninfected red mites and non-infecting fungus served as controls. In B. bassiana JEF-410, up-regulated gene expression was observed in tryptophan metabolism and secondary metabolite biosynthesis pathways. Genes related to acetyl-CoA synthesis were up-regulated in tryptophan metabolism, suggesting that energy metabolism and stress management were strongly activated. Secondary metabolites associated with fungal up-regulated DEGs were related to the production of substances toxic to insects such as beauvericin and beauveriolide, efflux pump of metabolites, energy production, and resistance to stress. In red mites, physical and immune responses that strengthen the cuticle against fungal infection were highly up-regulated. From these gene expression analyses, we identified essential factors for fungal infection and subsequent defenses of red mites. These results will serve as a strong platform for explaining the interaction between B. bassiana JEF-410 and red mites in the stage of active infection.
Topics: Animals; Poultry; Beauveria; Tryptophan; Mites; Trombiculidae; Chickens; Defense Mechanisms; Poultry Diseases; Mite Infestations
PubMed: 36800366
DOI: 10.1371/journal.pone.0280410 -
European Annals of Allergy and Clinical... Jan 2022To describe the prevalence of allergic sensitization to Storage Dust Mites (SDM), access whether the place of living and occupational exposure were determinants for SDM...
To describe the prevalence of allergic sensitization to Storage Dust Mites (SDM), access whether the place of living and occupational exposure were determinants for SDM sensitization and study association between Lepidoglyphus destructor and other SDM sensitization. Prospective analysis of patients evaluated for suspected allergic rhinitis and/or asthma that performed Skin Prick Tests (SPT) to SDM between January and December 2018 in our Department. Two hundred consecutive patients were evaluated for rhinitis and/or asthma in our outpatient consultation: 123 (61.5%) presented positivity for at least one SDM, 68.3% were female and the mean age was 33.1 ± 12.12. (69.9%) was the most prevalent, followed by (50.4%), and (48.8%) and (24.4%). Living in a rural place was not associated with a higher prevalence of sensitization to SDM, except for (p = 0.032), and working in a place with storage areas was not associated with sensitization to any of SDM. Sensitization to was associated with sensitization to , and (p less than 0.005), but not with Our study suggests that our population, independently of their occupational exposure and place of residency, are sensitized to SDM and that evaluation of sensitization to SDM should be considered as standard practice.
Topics: Acaridae; Adult; Allergens; Animals; Asthma; Dust; Female; Humans; Middle Aged; Mites; Prospective Studies; Pyroglyphidae; Skin Tests; Young Adult
PubMed: 33191715
DOI: 10.23822/EurAnnACI.1764-1489.178 -
Turkiye Parazitolojii Dergisi Sep 2023This study was carried out to detect house dust mites in houses and to investigate group 1 antigens of Dermatophagoid species in Ordu, Giresun, Trabzon and Rize...
OBJECTIVE
This study was carried out to detect house dust mites in houses and to investigate group 1 antigens of Dermatophagoid species in Ordu, Giresun, Trabzon and Rize provinces of the Central and Eastern Black Sea Region.
METHODS
Dust samples obtained from the beds were subjected to both microscopic and antigenic examination. Samples prepared by the lactic acid method for microscopic examination were evaluated under a light microscope. Antigenic analysis was performed by investigating Der p 1 and Der f 1 belonging to and by ELISA test.
RESULTS
90.3% of the dust samples were evaluated positive by microscopic examination (10x, 40x) and 149 mites were detected. 74%, 13%, spp. growth forms 5%, spp. 1%, 1%, 1%, 1%, 1% and unidentified mites were detected at the rate of 3% respectively. Der p 1 antigen was detected in 93% and Der f 1 antigen in 84.7%. The highest amount of antigen detected in one gram of powder was 1,272 μg for Der p 1 and 0,482 μg for Der f 1.
CONCLUSION
No difference was observed between mite species and distribution in the provinces where the study was conducted (p<0.05). Dermatophagoides were found in 93% of the population. The low (4%) rate of storage/food mites is related to the fact that samples were not taken from the floors. Antigen accumulation may be important in the beds since the activity of the mites is observed throughout the year in temperate and humid regions. It is thought that this diagnosis method can be used and can be taken into account in terms of the environments in which sensitive people live.
Topics: Humans; Animals; Pyroglyphidae; Prevalence; Dermatophagoides pteronyssinus; Dust
PubMed: 37724368
DOI: 10.4274/tpd.galenos.2023.35744 -
American Journal of Veterinary Research Sep 2001To identify the major allergenic proteins from the 3 main species of dust mites to which dogs react (Dermatophagoides farinae, D. pteronyssinus, and Euroglyphus maynei)...
OBJECTIVE
To identify the major allergenic proteins from the 3 main species of dust mites to which dogs react (Dermatophagoides farinae, D. pteronyssinus, and Euroglyphus maynei) and evaluate the potential cross-reactivity of dust mite allergens with antigens from the ectoparasitic mite Sarcoptes scabiei var canis.
SAMPLE POPULATION
Sera from 83 dogs with atopic dermatitis.
PROCEDURE
Sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoblotting using serum from atopic dogs was used to identify IgE-binding proteins in extracts of the 4 mite species.
RESULTS
Sera of atopic dogs contained IgE against 23, 17, 25, and 17 allergens from D. farinae, D. pteronyssinus, E. maynei, and S. scabiei, respectively. Unlike the situation for humans, the major allergens for dogs are mostly proteins that are larger than 90 kd molecular weight. Dermatophagoides farinae and E. maynei appear to be more allergenic for dogs than is D. pteronyssinus. Some dogs with serum IgE against dust mites also had IgE against antigens of S. scabiei var canis.
CONCLUSIONS AND CLINICAL RELEVANCE
Multiple dust mite allergens induce an IgE response in dogs. These allergens are mostly greater than 90 kd molecular weight.
Topics: Animals; Antigens, Dermatophagoides; Blotting, Western; Dog Diseases; Dogs; Dust; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Glycoproteins; Immunoglobulin E; Mites; Molecular Weight; Sarcoptes scabiei
PubMed: 11560257
DOI: 10.2460/ajvr.2001.62.1344 -
Scientific Reports Nov 2021Our study evaluated the association between Demodex infestation and recurrent hordeola and examined the clinical features associated with these eyelid lesions. This was... (Comparative Study)
Comparative Study Observational Study
Our study evaluated the association between Demodex infestation and recurrent hordeola and examined the clinical features associated with these eyelid lesions. This was an observational, comparative study. We reviewed 250 patients and divided them into the recurrent hordeolum (n = 153) and control (n = 97) groups. Demodex infestation was detected by epilating eyelashes around the lesion/s and viewing them under a light microscope. Patient medical records and photographs were retrospectively analyzed to identify the clinical characteristics of Demodex-associated recurrent hordeola. Demodex was detected in 91 (59.5%) and 17 (17.5%) patients in the recurrent hordeolum and control groups (p < 0.001), respectively. In the recurrent hordeolum group, Demodex mites were found in 74 (68.5%) and 17 (37.8%) of the adult and pediatric patients (p < 0.001), respectively. Among patients with recurrent hordeola, patients in their 20s were most likely to have concomitant Demodex infestation. Patients with Demodex infestations were also more likely to develop recurrent lesions within a shorter period of time from the primary incision and curettage. The most common presentation of Demodex-associated recurrent lesions was external hordeola (67%) (p = 0.002). Demodex infestation may cause recurrent hordeola in adults and children. These mites may play a greater role in the development of lesions in adult patients. The strongest association between Demodex infestation and recurrent lesions was seen in patients in their 20s. Our results suggest that if the hordeola recur within a short period of time with the clinical characteristics of external location of eyelid, multiple numbers of lesions, or anterior blepharitis, eyelash epilation should be performed to identify the presence of Demodex mites.
Topics: Adolescent; Adult; Aged; Animals; Child; Child, Preschool; Eye Infections, Parasitic; Female; Humans; Male; Middle Aged; Mite Infestations; Mites; Recurrence; Risk Factors; Young Adult
PubMed: 34725365
DOI: 10.1038/s41598-021-00599-7 -
Parasite (Paris, France) 2022The Chinese mole shrew, Anourosorex squamipes Milne-Edwards, 1872, is a common species of insectivorous mammal in Southwest China. Based on field investigations between...
The Chinese mole shrew, Anourosorex squamipes Milne-Edwards, 1872, is a common species of insectivorous mammal in Southwest China. Based on field investigations between 2001 and 2019, the present study reports the infestation of chiggers (larvae of chigger mites) on the shrew in Southwest China and certain ecology parameters for the first time. A total of 3169 chiggers were collected from 1694 A. squamipes and they were identified into 72 species and 10 genera in the family Trombiculidae. The overall infestation prevalence (P), mean abundance (MA) and mean intensity (MI) of A. squamipes with chiggers reached 11.1%, 1.87 and 16.86, respectively. The species diversity, species composition and infestation of chiggers on A. squamipes fluctuated in different environments (latitudes, altitudes, habitats and landscapes) and on different sexes and ages of the shrew hosts with high heterogeneity and low species similarity. In the established linear regression equation (M* = 0.173 + 1.054 M) for dominant mite Leptotrombidium densipunctatum, both the α and β values (α = 0.173, β = 1.054) exceeded the boundary values (F = 4.67, p < 0.05), and therefore the spatial distribution pattern of this mite was determined as an aggregated distribution among different individuals of shrew hosts. The species abundance distribution of the chigger community on A. squamipes conformed to the lognormal distribution, and its curve showed a gradually descending tendency from the rare mite species to the dominant mite species. The curve tendency of species-sample relationship implies that more species of chiggers would be found if the host samples infinitely keep increasing.
Topics: Altitude; Animals; China; Ecosystem; Humans; Mite Infestations; Mites; Shrews; Trombiculidae
PubMed: 35900094
DOI: 10.1051/parasite/2022038 -
PloS One 2015According to classic niche theory, species can coexist in heterogeneous environments by reducing interspecific competition via niche partitioning, e.g. trophic or...
According to classic niche theory, species can coexist in heterogeneous environments by reducing interspecific competition via niche partitioning, e.g. trophic or spatial partitioning. However, support for the role of competition on niche partitioning remains controversial. Here, we tested for spatial and trophic partitioning in feather mites, a diverse and abundant group of arthropods. We focused on the two dominant mite species, Microspalax brevipes and Zachvatkinia ovata, inhabiting flight feathers of the Cory's shearwater, Calonectris borealis. We performed mite counts across and within primary and tail feathers on free-living shearwaters breeding on an oceanic island (Gran Canaria, Canary Islands). We then investigated trophic relationships between the two mite species and the host using stable isotope analyses of carbon and nitrogen on mite tissues and potential host food sources. The distribution of the two mite species showed clear spatial segregation among feathers; M. brevipes showed high preference for the central wing primary feathers, whereas Z. ovata was restricted to the two outermost primaries. Morphological differences between M. brevipes and Z. ovata support an adaptive basis for the spatial segregation of the two mite species. However, the two mites overlap in some central primaries and statistical modeling showed that Z. ovata tends to outcompete M. brevipes. Isotopic analyses indicated similar isotopic values for the two mite species and a strong correlation in carbon signatures between mites inhabiting the same individual host suggesting that diet is mainly based on shared host-associated resources. Among the four candidate tissues examined (blood, feather remains, skin remains and preen gland oil), we conclude that the diet is most likely dominated by preen gland oil, while the contribution of exogenous material to mite diets is less marked. Our results indicate that ongoing competition for space and resources plays a central role in structuring feather mite communities. They also illustrate that symbiotic infracommunities are excellent model systems to study trophic ecology, and can improve our understanding of mechanisms of niche differentiation and species coexistence.
Topics: Animals; Bird Diseases; Birds; Feathers; Isotope Labeling; Mite Infestations; Mites; Symbiosis
PubMed: 26650672
DOI: 10.1371/journal.pone.0144728