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PloS One 2018In the blue crab, Callinectes sapidus, early studies suggested a relationship between smaller crabs, which molt more frequently, and higher rates of infection by the...
In the blue crab, Callinectes sapidus, early studies suggested a relationship between smaller crabs, which molt more frequently, and higher rates of infection by the dinoflagellate parasite, Hematodinium perezi. In order to better explore the influence of size and molting on infections, blue crabs were collected from the Maryland coastal bays and screened for the presence of H. perezi in hemolymph samples using a quantitative PCR assay. Molt stage was determined by a radioimmunoassay which measured ecdysteroid concentrations in blue crab hemolymph. Differences were seen in infection prevalence between size classes, with the medium size class (crabs 61 to 90 mm carapace width) and juvenile crabs (≤ 30 mm carapace width) having the highest infection prevalence at 47.2% and 46.7%, respectively. All size classes were susceptible to infection, although fall months favored disease acquisition by juveniles, whereas mid-sized animals (31-90 mm carapace width) acquired infection predominantly in summer. Disease intensity was also most pronounced in the summer, with blue crabs > 61 mm being primary sources of proliferation. Molt status appeared to be influenced by infection, with infected crabs having significantly lower concentrations of ecdysteroids than uninfected crabs in the spring and the fall. We hypothesize that infection by H. perezi may increase molt intervals, with a delay in the spring molt cycle as an evolutionary adaptation functioning to coincide with increased host metabolism, providing optimal conditions for H. perezi propagation. Regardless of season, postmolt crabs harbored significantly higher proportions of moderate and heavy infections, suggesting that the process of ecdysis, and the postmolt recovery period, has a positive effect on parasite proliferation.
Topics: Animals; Crustacea; Dinoflagellida; Molting; Polymerase Chain Reaction; Radioimmunoassay; Seasons
PubMed: 29474370
DOI: 10.1371/journal.pone.0192237 -
Journal of Insect Science (Online) Jul 2021Chitin deacetylases (CDAs) are chitin-degrading enzymes that play a key role in insect molting. In this study, we identified and characterized four full-length cDNAs of...
Chitin deacetylases (CDAs) are chitin-degrading enzymes that play a key role in insect molting. In this study, we identified and characterized four full-length cDNAs of CDAs from Sogatella furcifera (Horváth). Developmental expression showed that SfCDA1 and SfCDA2 were expressed at all nymph developmental stages, SfCDA3 and SfCDA4 were mainly expressed in the third-instar to fifth-instar nymph stages, whereas tissue-specific analyses indicated that four CDA genes were mainly high expressed in the integument and head during the fifth-instar nymph. RNA interference (RNAi) results revealed that SfCDA1, SfCDA2, and SfCDA4 are associated with molting defect and high mortality with nymph-adult molting. Furthermore, transcripts of chitin synthase 1 variants (SfCHS1, SfCHS1a, and SfCHS1b) were significantly downregulated and causing significant changes in the expression levels of trehalases (TRE1 and TRE2) in the SfCDA1, SfCDA2, and SfCDA4 dsRNA treatment groups. By contrast, no significant phenotypic characteristics were observed after dsSfCDA3 injection. Taken together, our results suggest that SfCDA1, SfCDA2, and SfCDA4 play a vital role in nymph-adult transition, and these genes could regulate chitin biosynthesis expression levels.
Topics: Amidohydrolases; Animals; Chitin; DNA, Complementary; Genes, Insect; Hemiptera; Insect Proteins; Molting; Nymph; Phylogeny; RNA Interference; Wings, Animal
PubMed: 34333649
DOI: 10.1093/jisesa/ieab051 -
Poultry Science Sep 2019Physiological changes during molt and postmolt egg production variables were compared among hens induced to molt by using cassava meal varying the length of the feeding...
Physiological changes during molt and postmolt egg production variables were compared among hens induced to molt by using cassava meal varying the length of the feeding period. Hens were randomly assigned to 3 treatments of 72 birds each: 1) molted by full feeding with cassava meal for 4 wk (CAS4); 2) molted by full feeding with cassava meal for 5 wk (CAS5); and 3) molted by full feeding with cassava meal for 6 wk (CAS6). All groups were fed a pullet developer diet for 2 wk following treatment. During the molt period, the birds were exposed to an 8L:16D photoperiod and had access to drinking water at all times. Thereafter, all hens were fed a layer diet (17.71% CP), and exposed to a 16L:8D photoperiod, and production performance was measured for 16 wk. The molt treatments resulted in total cessation of egg production in all treatments within 2 wk following feeding of the molt diet. BW loss of birds in the CAS6 treatment was approximately 41.81%, which was significantly higher (P < 0.05) than those in the CAS5 (37.63%) and CAS4 (31.89%) treatments. The CAS5 and CAS6 hens had significantly lower (P < 0.05) ovarian and oviducal weights than the CAS4 hens. No consistent differences were observed for concentrations of plasma estradiol and progesterone, bone quality, or mortality among the treatments. Postmolt feed consumption, egg weight, and mortality were not different (P > 0.05) as a result of molt induction method. However, the CAS4 hens had significantly higher (P < 0.05) cumulative egg production and lower feed conversion ratio than hens in the CAS6 treatment. Significant differences were found among treatments for Haugh units and shell thickness in some periods of the postmolt. It was concluded that cassava meal could be used to induce molt in laying hens effectively, feeding the molt diet for 4 wk produced optimum body weight loss and postmolt performance.
Topics: Animal Feed; Animals; Chickens; Diet; Female; Manihot; Molting; Random Allocation; Time Factors
PubMed: 30915458
DOI: 10.3382/ps/pez110 -
Poultry Science Nov 2016The aim of this study was to investigate the efficacy of feed-grade preparations of mannan oligosaccharides ( MOS: ) and oregano essential oil ( OEO: ) in forced molted... (Comparative Study)
Comparative Study
Comparative evaluation of dietary supplementation with mannan oligosaccharide and oregano essential oil in forced molted and fully fed laying hens between 82 and 106 weeks of age.
The aim of this study was to investigate the efficacy of feed-grade preparations of mannan oligosaccharides ( MOS: ) and oregano essential oil ( OEO: ) in forced molted or fully fed 82-week-old, laying hens. A 2 × 3 factorial experiment investigated the influence of molting vs. full feeding and dietary supplements [i.e., unsupplemented control, MOS (1 g/kg) diet, and OEO (24 mg/kg) diet] on production parameters, egg quality, serum stress indicators, blood constituents, tibial characteristics, liver antioxidant status, and cecal microflora composition. A total of 864 Single Comb White Leghorn hens were randomly assigned to 6 treatments, each with 6 replicates of 24 hens each, and studied for 25 wk. Hens were fed a molt diet containing of 50% alfalfa and 50% wheat bran ( AA+WB: ) for 12 d, then returned to the laying ration. Results indicate that molt vs. full feed impacted more on most variables measured than supplementation or supplement type. Significant (P < 0.01) interactions between molting and diet were observed for the egg production, egg weight, egg mass, and feed conversion ratio ( FCR: ). In fully fed hens, MOS supplementation improved (P < 0.01) the egg production, egg weight, and FCR, and an OEO addition significantly improved the egg production and FCR in forced molted hens. Molting improved egg quality despite the significant regression in ovary and oviduct weight (P < 0.01), though supplements showed no influence. The bone ash (P < 0.01) and mineral content (P < 0.05) of molted hens were significantly lower than those of fully fed counterparts; however, poor mineralization was not reflected in the bones' mechanical properties. No significant differences were observed among treatments for hematological characteristics. Both the MOS and particularly the OEO supplementation improved (P < 0.01) liver antioxidant status and mitigated the significant increase in cecal pathogenic bacteria after molt. Our results indicate that full feeding with an aa+wb diet is an effective non-feed-removal method for molted hens, the benefit of which can be improved with MOS and OEO supplementation.
Topics: Animal Feed; Animals; Chickens; Diet; Female; Mannans; Molting; Oils, Volatile; Oligosaccharides; Origanum; Prebiotics; Random Allocation
PubMed: 27143766
DOI: 10.3382/ps/pew140 -
Current Biology : CB Jun 1996The discovery of a new insect peptide hormone that triggers ecdysis - shedding of an old cuticle - has revealed hidden layers of intricacy about an insect behavior... (Review)
Review
The discovery of a new insect peptide hormone that triggers ecdysis - shedding of an old cuticle - has revealed hidden layers of intricacy about an insect behavior previously thought to be mediated by a single neuropeptide.
Topics: Animals; Insect Hormones; Insecta; Intercellular Signaling Peptides and Proteins; Manduca; Models, Neurological; Molting; Peptides
PubMed: 8793284
DOI: 10.1016/s0960-9822(09)00439-4 -
Philosophical Transactions of the Royal... Oct 2019The three modes of insect postembryonic development are ametaboly, hemimetaboly and holometaboly, the latter being considered the only significant metamorphosis mode....
The three modes of insect postembryonic development are ametaboly, hemimetaboly and holometaboly, the latter being considered the only significant metamorphosis mode. However, the emergence of hemimetaboly, with the genuine innovation of the final moult, represents the origin of insect metamorphosis and a necessary step in the evolution of holometaboly. Hemimetaboly derives from ametaboly and might have appeared as a consequence of wing emergence in Pterygota, in the early Devonian. In extant insects, the final moult is mainly achieved through the degeneration of the prothoracic gland (PG), after the formation of the winged and reproductively competent adult stage. Metamorphosis, including the formation of the mature wings and the degeneration of the PG, is regulated by the MEKRE93 pathway, through which juvenile hormone precludes the adult morphogenesis by repressing the expression of transcription factor E93, which triggers this change. The MEKRE93 pathway appears conserved in extant metamorphosing insects, which suggest that this pathway was operative in the Pterygota last common ancestor. We propose that the final moult, and the consequent hemimetabolan metamorphosis, is a monophyletic innovation and that the role of E93 as a promoter of wing formation and the degeneration of the PG was mechanistically crucial for their emergence. This article is part of the theme issue 'The evolution of complete metamorphosis'.
Topics: Animals; Biological Evolution; Insecta; Molting
PubMed: 31438822
DOI: 10.1098/rstb.2018.0415 -
Poultry Science Jan 2004Two commercial strains, Hy-Line W-36 and DeKalb XL, were moved to a laying house at 18 wk of age. They were housed 6 hens/layer cage at 2 densities (361 and 482...
Two commercial strains, Hy-Line W-36 and DeKalb XL, were moved to a laying house at 18 wk of age. They were housed 6 hens/layer cage at 2 densities (361 and 482 cm2/bird) with 2 replications each per strain/density combination. The high-density treatment contained 24 hens/replication and the low-density treatment contained 18 hens/replication for a total of 168 hens. Production parameters were measured during the first egg production cycle, the molt period, and the first 4 wk of the second lay cycle (20 to 68 wk of age). Behavioral observations were taken during 2 consecutive d at 26, 34, 43, 51, 62, 64, and 68 wk of age to examine behavioral patterns. Modified Hansen's tests were conducted concurrently to provide indication of the fearfulness levels of hens at the various stages of production. The production characteristics were similar for both strains. The hens kept at the higher density had lower (P < 0.01) hen-day production and (P < 0.05) daily egg mass. Appetitive behaviors were not affected by strain or density but were affected by the age of the hen and by molting. During the molt, feeding and drinking behavioral acts were fewer (P < 0.05) at 0.018 and 0.013 acts per bird/min, respectively, and standing behavior was highest. The results indicated that the frequencies of pecking inedible objects during the molt period were similar to the frequencies at 26 and 34 wk. Hens performed more acts of standing, and crouching and had lower frequency of movement during the molt. Those kept at a low density performed more movement acts. Feather pecking decreased as hens aged and increased when they molted but was not affected by strain or density. The frequency of aggression and submissive acts was significantly lower during the molt period. Behaviors were affected by strain, density, bird age, and molting; however, the patterns and number of aggressive acts did not increase to compromise the welfare status of the hens. Behaviors during the molt appeared consistent with mechanisms for conservation of body reserves.
Topics: Age Factors; Aging; Animal Husbandry; Animal Welfare; Animals; Behavior, Animal; Chickens; Feathers; Female; Housing, Animal; Molting; Oviposition; Population Density; Random Allocation
PubMed: 14761079
DOI: 10.1093/ps/83.1.15 -
Physiology & Behavior Feb 2019While endotherms can rely on their insulation to reduce heat loss to adapt to cold environments, renewing of fur during molt impairs insulation while they have to...
While endotherms can rely on their insulation to reduce heat loss to adapt to cold environments, renewing of fur during molt impairs insulation while they have to perfuse the periphery to support epidermal tissues. The southern elephant seal Mirounga leonina undertakes an annual catastrophic molt while fasting on land in a wet, windy and cold environment. However, southern elephant seals show characteristic aggregation patterns that are predicted to reduce high metabolic costs during the molt. Between 2012 and 2016, 59 female elephant seals were tracked on land during their molt to study their aggregation behavior in relation to molt stage, habitat type and local weather conditions. Infrared thermography and stomach temperature loggers were used to observe variation in body surface and internal temperature in relation to molt stage and aggregation behavior. We found that thermal constraints varied during the molt, with a peak in surface temperature during the mid-stage of the molt. Wallows (mud pools) appear as favorable habitat to aggregate while molting. Indeed, wallows offered a warmer microclimate with greater ground temperature and lower wind speed. Moreover, there was a greater proportion of aggregated seals and larger group size in wallows. These aggregation patterns in wallows were influenced by local weather such that a greater proportion of seals were located in the center of the aggregation, and larger group size occurred during days of unfavorable meteorological conditions. We also observed a higher proportion of seals at mid-stage of molt amongst aggregated seals compared to isolated individuals. This aggregation behavior may reduce the cost of thermogenesis as surface body temperature and stomach temperature were cooler by 1.0 °C and 1.5 °C, respectively, in aggregated compared to isolated seals. As a consequence, huddling behavior may be thermally advantageous for female southern elephant seals during the molt.
Topics: Animals; Behavior, Animal; Body Temperature; Body Temperature Regulation; Ecosystem; Environment; Female; Molting; Seals, Earless; Temperature
PubMed: 30385351
DOI: 10.1016/j.physbeh.2018.10.016 -
PloS One 2019Biological rhythms regulate innumerable physiological processes, yet little is known of factors that regulate many of these rhythms. Disruption in the timing of these...
Biological rhythms regulate innumerable physiological processes, yet little is known of factors that regulate many of these rhythms. Disruption in the timing of these rhythms can have devastating impacts on population sustainability. We hypothesized that the timing of the molt infradian rhythm in the crustacean Daphnia magna is regulated by the joint action of the protein E75 and nitric oxide. Further, we hypothesized that disruption of the function of E75 would adversely impact several physiological processes related to growth and reproduction. Analysis of mRNA levels of several genes, involved in regulating the molt cycle in insects, revealed the sequential accumulation of E75, its dimer partner HR3, FTZ-F1, and CYP18a1 during the molt cycle. Exposure to the nitric oxide donor sodium nitroprusside early in the molt cycle had no effect on E75 or HR3 mRNA levels, but delayed the peak accumulation of FTZ-F1 and CYP18a1 mRNA. The subsequent exuviation was also delayed consistent with the delay in peak accumulation of FTZ-F1 and CYP18a1. These results supported our assertion that nitric oxide binds E75 rendering it incapable of binding HR3. Excess HR3 protein then enhanced the accumulation of the downstream products FTZ-F1 and CYP18a1. Similarly, suppression of E75 mRNA levels, using siRNA, had no effect on mRNA levels of HR3 but elevated mRNA levels of FTZ-F1. Consistent with these molecular responses, the suppression of E75 using siRNA increased the duration of the molt cycle and reduced the number of offspring produced. We conclude that the molt cycle of daphnids is regulated in a manner similar to insects and disruption of E75 results in a lengthening of the molt cycle and a reduction the release of viable offspring.
Topics: Animals; Daphnia; Gene Expression Regulation, Developmental; Models, Biological; Molting; Nitroprusside; RNA Interference; RNA, Double-Stranded; RNA, Messenger; Receptors, Cytoplasmic and Nuclear; Signal Transduction
PubMed: 31454379
DOI: 10.1371/journal.pone.0221642 -
PloS One 2012Food limitation greatly affects bird breeding performance, but the effect of nutritive stress on molt has barely been investigated outside of laboratory settings. Here...
Food limitation greatly affects bird breeding performance, but the effect of nutritive stress on molt has barely been investigated outside of laboratory settings. Here we show changes in molting patterns for an entire native Hawaiian bird community at 1650-1900 m elevation on the Island of Hawaii between 1989-1999 and 2000-2006, associated with severe food limitation throughout the year beginning in 2000. Young birds and adults of all species took longer to complete their molt, including months never or rarely used during the 1989-1999 decade. These included the cold winter months and even the early months of the following breeding season. In addition, more adults of most species initiated their molt one to two months earlier, during the breeding season. Suspended molt, indicated by birds temporarily not molting primary flight feathers during the months of peak primary molt, increased in prevalence. Food limitation reached the point where individuals of all species had asymmetric molt, with different primary flight feathers molted on each wing. These multiple changes in molt, unprecedented in birds, had survival consequences. Adult birds captured during January to March, 2000-2004, had lower survival in four of five species with little effect of extended molt. Extended molt may be adaptive for a nutrient stressed bird to survive warm temperatures but not cool winter temperatures that may obliterate the energy savings. The changing molt of Hawaiian birds has many implications for conservation and for understanding life history aspects of molt of tropical birds.
Topics: Analysis of Variance; Animals; Birds; Feathers; Female; Hawaii; Male; Molting; Reproduction; Seasons; Species Specificity; Temperature; Time Factors; Trees; Wings, Animal
PubMed: 22279547
DOI: 10.1371/journal.pone.0029834