-
Journal of Clinical Microbiology Aug 2004Mucoid strains of Moraxella nonliquefaciens were recovered from the sputa of three indigenous Australians with chronic lung disease. These atypical strains failed to...
Mucoid strains of Moraxella nonliquefaciens were recovered from the sputa of three indigenous Australians with chronic lung disease. These atypical strains failed to reduce nitrate, and one strain produced beta-lactamase. While the mucoid phenotype of M. nonliquefaciens has rarely been reported, the mucoid nitrate-negative biovar has never been previously reported.
Topics: Adolescent; Australia; Chronic Disease; Diagnosis, Differential; Female; Humans; Lung Diseases, Fungal; Male; Middle Aged; Molecular Sequence Data; Moraxella; Moraxellaceae Infections; Native Hawaiian or Other Pacific Islander; Nitrates
PubMed: 15297558
DOI: 10.1128/JCM.42.8.3888-3890.2004 -
Journal of Clinical Microbiology Sep 1987Four murine monoclonal antibodies which reacted with a (2----8)alpha-linked sialic acid polysaccharide were produced. Three of the antibodies reacted specifically with...
Four murine monoclonal antibodies which reacted with a (2----8)alpha-linked sialic acid polysaccharide were produced. Three of the antibodies reacted specifically with Neisseria meningitidis serogroup B and Escherichia coli K-1 polysaccharide antigens, whereas one antibody cross-reacted with N. meningitidis group C polysaccharide antigen, a (2----9)alpha-linked homopolymer of sialic acid. By using the most avid antibody (MB 62), a latex particle agglutination test was developed which could detect capsular polysaccharide at 10 ng/ml. It also detected antigen in the cerebrospinal fluid (CSF) of all seven N. meningitidis group B- and two E. coli K-1-infected patients, whereas 57 control CSF samples, including 8 from neonates, were negative. Cultures of 21 N. meningitidis group B strains, 7 E. coli K-1 strains, and 1 Moraxella nonliquefaciens strain gave a positive result, whereas 53 strains from other serogroups were all negative. In a separate clinical evaluation, the overall sensitivity of the latex particle agglutination test was 81% (22 of 27) with fresh CSF samples, 48% (15 of 31) with stored CSF samples, and 94% (32 of 34) with blood cultures. No false-positive reactions were recorded with 165 control CSF samples, and the specificity with blood cultures was 99.4% (519 of 522).
Topics: Agglutination Tests; Animals; Antibodies, Bacterial; Antibodies, Monoclonal; Antigens, Bacterial; Bacterial Capsules; Cerebrospinal Fluid; Counterimmunoelectrophoresis; Cross Reactions; Escherichia coli; Escherichia coli Infections; Humans; Infant, Newborn; Latex Fixation Tests; Meningitis, Meningococcal; Mice; Moraxella; Neisseria meningitidis; Polysaccharides, Bacterial; Predictive Value of Tests
PubMed: 3116036
DOI: 10.1128/jcm.25.9.1641-1644.1987