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Evaluation of the MYCOPLASMA IST3 urogenital mycoplasma assay in an international multicentre trial.The Journal of Antimicrobial... Nov 2021To evaluate the accuracy, susceptibility and specificity of MYCOPLASMA IST3, the next generation of the most popular culture-based in vitro diagnostic device designed to...
OBJECTIVES
To evaluate the accuracy, susceptibility and specificity of MYCOPLASMA IST3, the next generation of the most popular culture-based in vitro diagnostic device designed to detect, identify and test the susceptibility of urogenital mycoplasma infections.
METHODS
MYCOPLASMA IST3 was evaluated against culture- and molecular-based gold standard methodologies to detect, identify, enumerate and determine antimicrobial resistance for Mycoplasma hominis and Ureaplasma species in 516 clinical samples collected across France, Serbia and the UK. Sample types included vulvovaginal/endocervical or urethral swabs (dry swab or eSwab®), semen and urine samples, which included blinded analysis following addition of a panel of 80 characterized control strains.
RESULTS
Overall species identification was excellent for both Ureaplasma spp. (98.4% sensitivity, 99.7% specificity) and M. hominis (95.7% sensitivity, 100% specificity) relative to combined colony morphology on agar and quantitative PCR standards. Non-dilution-based bacterial load estimation by the assay was accurate between 83.7% (M. hominis) and 86.3% (Ureaplasma spp.) of the time (increased to 94.2% and 100%, respectively, if ±10-fold variance was allowed) relative to colonies counted on agar. Resistance accuracy for Ureaplasma spp. varied from gold standards for only 11/605 of individual tests (major error rate = 1.8%) and for 14/917 individual tests for M. hominis (major error rate = 1.5%).
CONCLUSIONS
The redesigned MYCOPLASMA IST3 assay eliminated previous shortcomings by providing independent accurate resistance screening of M. hominis and Ureaplasma species, even in mixed infections, with CLSI-compliant thresholds. Specificity, sensitivity and enumeration estimates correlated closely with the confirmatory methods.
Topics: Humans; Microbial Sensitivity Tests; Mycoplasma; Mycoplasma Infections; Mycoplasma hominis; Ureaplasma; Ureaplasma Infections; Ureaplasma urealyticum
PubMed: 34477840
DOI: 10.1093/jac/dkab320 -
BMC Infectious Diseases Nov 2007Genital ureaplasmas (Ureaplasma urealyticum and Ureaplasma parvum) and mycoplasmas (Mycoplasma genitalium and Mycoplasma hominis) are potentially pathogenic species...
BACKGROUND
Genital ureaplasmas (Ureaplasma urealyticum and Ureaplasma parvum) and mycoplasmas (Mycoplasma genitalium and Mycoplasma hominis) are potentially pathogenic species playing an etiologic role in both genital infections and male infertility. Reports are, however, controversial regarding the effects of these microorganisms infections in the sperm seminological variables. This study aimed at determining the frequency of genital ureplasmas and mycoplasmas in semen specimens collected from infertile men, and at comparing the seminological variables of semen from infected and non-infected men with these microorganisms.
METHODS
A total of 120 semen samples collected from infertile men were investigated. Semen specimens were examined by in-house PCR-microtiter plate hybridization assay for the presence of genital ureaplasmas and mycoplasmas DNA. Semen analysis was assessed according to the guidelines of the World Health Organization. Standard parametric techniques (t-tests) and nonparametric techniques (Wilcoxon tests) were used for statistical analysis.
RESULTS
The frequency of genital ureaplasmas and mycoplasmas detected in semen samples of infertile men was respectively 19.2% (23/120) and 15.8% (19/120). The frequency of Ureaplasma urealyticum (15%) was higher than that of Mycoplasma hominis (10.8%), Ureaplasma parvum (4.2%) and Mycoplasma genitalium (5%). Mixed species of mycoplasmas and ureaplasmas were detected in 6.7% of semen samples. Comparison of the parameters of the standard semen analysis between the male partners of the infertile couples with and without genital ureaplasmas and mycoplasmas infection showed that the presence of Mycoplasma hominis DNA in semen samples is associated with low sperm concentration (p = 0.007) and abnormal sperm morphology (p = 0.03) and a negative correlation between sperm concentration and the detection of Mycoplasma genitalium in semen samples of infertile men (p = 0.05). The mean values of seminal volume, pH, vitality, motility and leukocyte count were not significantly related either to the detection of genital mycoplasmas DNA or to the detection of ureaplasmas DNA in semen specimens.
CONCLUSION
Our results demonstrate that genital mycoplasmas and ureaplasmas seem to be widespread among the male partners of infertile couples in Tunisia. Genital mycoplasmas infections of the male genital tract could negatively influence semen quality. Our results also indicate that PCR-microtiter plate hybridization assay method provides a rapid and effective technique to detect human genital mycoplasmas and ureaplasmas which is useful for etiological and epidemiological studies of these pathogens.
Topics: Adult; DNA, Bacterial; Genital Diseases, Male; Humans; Infertility, Male; Male; Middle Aged; Mycoplasma Infections; Mycoplasma genitalium; Mycoplasma hominis; Polymerase Chain Reaction; Prevalence; Semen; Tunisia; Ureaplasma; Ureaplasma Infections; Ureaplasma urealyticum
PubMed: 17988404
DOI: 10.1186/1471-2334-7-129 -
International Journal of Reproductive... Mar 2016Mycoplasma infections are suggested as etiology of adverse pregnancy outcomes.
BACKGROUND
Mycoplasma infections are suggested as etiology of adverse pregnancy outcomes.
OBJECTIVE
The aim of this study was to evaluate the association of Mycoplasma hominis (M. hominis) infection and spontaneous abortion among pregnant women.
MATERIALS AND METHODS
In this case-control study that was conducted from August 2012 to January 2013, totally, 109 women were included with spontaneous abortion with gestational ages of 10-20 weeks (Cases), and 109 women with normal pregnancy with gestational ages between 20-37 weeks (Controls) in Sanandaj, Iran. Using specific primers and extracted DNA from endocervical swabs, a PCR test was conducted for detection of M. hominis infection in women. For comparison of qualitative and quantitative variables, independent Fisher tests were used and p<0.05 was considered significant.
RESULTS
The total frequency of M. hominis infection was 6 (2.75%) in women. The frequency of M. hominis infection was 2 (1.83%) in the case group (spontaneous abortion) and 4 (3.66%) in the control group, respectively. In both case and control groups, no association was seen between M.hominis infection and spontaneous abortion (OR=0. 49, CI 95%: 0.08-2.73, p=0. 683).
CONCLUSION
M. hominis was positive in the genital tract of some pregnant women, but it was not associated with spontaneous abortion. However, to prevent adverse pregnancy outcomes in women, foetus and neonate, routine screening and treatment for the genital Mycoplasma is recommended.
PubMed: 27294216
DOI: No ID Found -
Cancers Apr 2020Tanzania faces one of the highest cervical cancer burdens in the world. Recent work has suggested that the bacterial family is associated with higher levels of human...
Tanzania faces one of the highest cervical cancer burdens in the world. Recent work has suggested that the bacterial family is associated with higher levels of human papillomavirus (HPV), human immunodeficiency virus (HIV), and pre-cancerous cervical lesions. infection in Tanzania is not well understood, especially when considering the differences between sexually transmitted species of . To establish the prevalence of common cervical infections and evaluate their relationship with risk factors for cervical cancer, 1160 Tanzanian women responded to an epidemiological questionnaire and were tested for HIV, HPV, cervical lesions, , , spp., and . A subset of 134 women were used for 16s metagenomic sequencing of cervical DNA to establish the relative abundance of and present. PCR detection of bacteria at the cervix found spp. in 51.4% of women, in 34%, in 2.3%, and in 75.6%. and infection were significantly more prevalent among women with HPV and HIV. prevalence was similar despite severity of cervical lesions; however, abundance of increased significantly in women with cervical lesions. These results emphasize the importance of understanding the relationship between and HPV-related cervical pathogenesis.
PubMed: 32353967
DOI: 10.3390/cancers12051093 -
Parasitology Research Mar 2018In Europe, up to 90% of isolated Trichomonas vaginalis strains are naturally infected with Mycoplasma hominis, a facultative pathogen of the human genital tract. The...
In Europe, up to 90% of isolated Trichomonas vaginalis strains are naturally infected with Mycoplasma hominis, a facultative pathogen of the human genital tract. The consequences of this endosymbiosis are not yet well understood. The aim of the current study was to evaluate the impact of natural and artificial infections with M. hominis on the RNA expression levels of metronidazole susceptibility-associated genes of T. vaginalis. Three T. vaginalis strains (TVSS10-, TVSS25-, G3) without M. hominis, as well as the same strains naturally (TVSS10+, TVSS25+) and artificially (G3-MhSS25, TVSS25-MhSS25) infected with M. hominis, were investigated for their expression profiles of three genes associated with metronidazole resistance (ferredoxin, flavin reductase 1 and pyruvate:ferredoxin oxidoreductase). The minimal inhibitory concentrations (MICs) of metronidazole were evaluated for all combinations and the respective M. hominis-free T. vaginalis strains were used as controls. The sole presence of M. hominis led to a down-regulation of metronidazole susceptibility-associated genes in all T. vaginalis strains tested. Interestingly, the effect was more prominent in the artificial symbioses. Moreover, a twofold enhancement of metronidazole tolerability was observed in three infected T. vaginalis strains, compared to the respective strains without M. hominis. In conclusion, M. hominis had an impact on gene expression in all T. vaginalis strains and on metronidazole MIC in all but one strain tested.
Topics: Antiprotozoal Agents; Down-Regulation; Drug Resistance; Europe; Gene Expression Regulation; Metronidazole; Microbial Sensitivity Tests; Mycoplasma hominis; Symbiosis; Trichomonas vaginalis
PubMed: 29368037
DOI: 10.1007/s00436-018-5761-6 -
Journal of Clinical Pathology Jul 1967One hundred and seventyone antenatal patients were examined for the presence of ;large colony' mycoplasmas in the vagina, and for complement-fixing antibody to...
One hundred and seventyone antenatal patients were examined for the presence of ;large colony' mycoplasmas in the vagina, and for complement-fixing antibody to Mycoplasma hominis. In 25 patients the findings before and after delivery were compared. In patients from whom M. hominis was grown, antibody was twice as common after delivery, and the development of antibody was sometimes associated with pyrexia and signs of genital tract infection.
Topics: Adolescent; Adult; Antibodies; Antibody Formation; Complement Fixation Tests; Female; Genital Diseases, Female; Humans; Mycoplasma; Mycoplasma Infections; Pregnancy; Pregnancy Complications, Infectious; Puerperal Infection; Vagina
PubMed: 5602580
DOI: 10.1136/jcp.20.4.633 -
Medicine May 2023Postoperative intracranial mycoplasma hominis infection was a rare complication. Timely diagnosis was difficult due to its growth characteristics and nonspecific...
RATIONALE
Postoperative intracranial mycoplasma hominis infection was a rare complication. Timely diagnosis was difficult due to its growth characteristics and nonspecific clinical symptoms.
PATIENT CONCERNS
A 52-year-old man underwent bilateral decompressive craniotomy for severe traumatic brain injury. On the seventeenth day after surgery, the patient developed an unexplained high fever. Empirical anti-infective therapy was ineffective, and the fever persisted. In addition, viscous pus oozed from the head incision. Empiric therapy was still ineffective, the fever persisted, and the culture result was negative. The lumbar puncture pressure was 150 mmH2O and the cerebrospinal fluid white blood cell count was 3600 × 106/L, suggesting an intracranial infection.
DIAGNOSES
Culture growth morphologically consistent with mycoplasma species was obtained from multiple specimens (scalp incision fluid and cerebrospinal fluid) and the identification of mycoplasma hominis was confirmed by 16S rDNA sequencing.
INTERVENTION
Targeted anti-infective therapy (Minocycline), change of fresh wound dressing, and continued lumbar cerebrospinal fluid drainage.
OUTCOME
At the 3-month follow-up, the patient was still in the rehabilitation department of the local hospital for treatment, but there were no symptoms of intracranial infection.
LESSONS
Neurosurgeons should carefully examine postoperative incisions and be aware of the possibility of mycoplasma infection during clinical management.
Topics: Male; Humans; Middle Aged; Mycoplasma hominis; Decompressive Craniectomy; Meningitis; Postoperative Complications; Epidural Abscess; Mycoplasma Infections; Surgical Wound; Empyema; Craniotomy
PubMed: 37171345
DOI: 10.1097/MD.0000000000033745 -
Journal of Laboratory Physicians Dec 2021A 21-year-old human immunodeficiency virus-positive male patient presented with complaints of multiple hyperpigmented verrucous papules over his perianal area. He...
A 21-year-old human immunodeficiency virus-positive male patient presented with complaints of multiple hyperpigmented verrucous papules over his perianal area. He reported having unprotected anal and oral sex with multiple male partners. On examination, superficial ill-defined perianal erosions were present. A first void urine sample and clinician-collected rectal and oropharyngeal swabs were sent for the detection of , and spp. Rectal swab tested positive for all the four pathogens. Oropharyngeal swab and urine samples tested positive for . The patient was treated with doxycycline and moxifloxacin. This case underscores the importance of screening of men who have sex with men for possible coinfections with multiple sexually transmitted pathogens.
PubMed: 34975260
DOI: 10.1055/s-0041-1731119 -
BMJ Open Nov 2018Several bacterial sexually transmitted and genital mycoplasma infections during pregnancy have been associated with poor pregnancy and perinatal outcomes. Comprehensive...
INTRODUCTION
Several bacterial sexually transmitted and genital mycoplasma infections during pregnancy have been associated with poor pregnancy and perinatal outcomes. Comprehensive and systematic information about associations between sexually transmitted infections (STI) and genital infections in pregnancy and adverse perinatal outcomes is needed to improve understanding about the evidence for causal associations between these infections and adverse pregnancy and neonatal outcomes. Our primary objective is to systematically review the literature about associations between: (1) in pregnancy and preterm birth; (2) in pregnancy and preterm birth; (3) and/or in pregnancy and preterm birth.
METHODS AND ANALYSIS
We will undertake a systematic search of Medline, Excerpta Medica database and the Cochrane Library and Cumulative Index to Nursing and Allied Health Literature. Following an initial screening of titles by one reviewer, abstracts will be independently assessed by two reviewers before screening of full-text articles. To exclude a manuscript, both reviewers need to agree on the decision. Any discrepancies will be resolved by discussion, or the adjudication of a third reviewer. Studies will be included if they report testing for one or more of during pregnancy and report pregnancy and/or birth outcomes. In this review, the primary outcome is preterm birth. Secondary outcomes are premature rupture of membranes, low birth weight, spontaneous abortion, stillbirth, neonatal mortality and ophthalmia neonatorum. We will use standard definitions, or definitions reported by study authors. We will examine associations between exposure and outcome in forest plots, using the I statistic to examine between study heterogeneity. Where appropriate, we will use meta-analysis to combine results of individual studies.
ETHICS AND DISSEMINATION
This systematic review of published literature does not require ethical committee approval. Results of this review will be published in a peer reviewed, open access journal.
PROSPERO REGISTRATION NUMBER
CRD42016050962.
Topics: Female; Humans; Infant, Newborn; Pregnancy; Bacterial Infections; Gram-Negative Bacteria; Mycoplasma genitalium; Mycoplasma hominis; Neisseria gonorrhoeae; Pregnancy Complications, Infectious; Pregnancy Outcome; Premature Birth; Research Design; Sexually Transmitted Diseases; Ureaplasma; Ureaplasma urealyticum; Meta-Analysis as Topic; Systematic Reviews as Topic
PubMed: 30498048
DOI: 10.1136/bmjopen-2018-024175 -
Frontiers in Cellular and Infection... 2023Extra-urogenital infections due to () are rare, particularly co-infection with (). Herein, we report on a patient who was co-infected and successfully treated despite...
BACKGROUND
Extra-urogenital infections due to () are rare, particularly co-infection with (). Herein, we report on a patient who was co-infected and successfully treated despite delayed treatment.
CASE PRESENTATION
We reported the case of a 43-year-old man with and co-infection after a traffic accident. The patient developed a fever and severe infection despite postoperative antimicrobial therapies. The blood culture of wound tissues was positive for Meanwhile, culturing of blood and wound samples showed pinpoint-sized colonies on blood agar plates and fried-egg-type colonies on mycoplasma medium, which were identified as by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA sequencing. Based on antibiotic susceptibility and symptoms, ceftazidime-avibactam and moxifloxacin were administered for infection. Meanwhile, after the failure of a series of anti-infective agents, and co-infection was successfully treated with a minocycline-based regimen and polymyxin B.
CONCLUSION
The co-infection with and was successfully treated with anti-infective agents despite delayed treatment, providing information for the management of double infection.
Topics: Male; Humans; Adult; Pseudomonas aeruginosa; Mycoplasma hominis; Mycoplasma Infections; RNA, Ribosomal, 16S; Coinfection; Anti-Infective Agents; Pseudomonas Infections; Anti-Bacterial Agents
PubMed: 37197207
DOI: 10.3389/fcimb.2023.1159891