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Scientific Reports Nov 2022This study analyzed severe acute endometritis action on myometrial density and distribution of protein gene product (PGP)9.5- and calcitonin gene-related peptide...
This study analyzed severe acute endometritis action on myometrial density and distribution of protein gene product (PGP)9.5- and calcitonin gene-related peptide (CGRP)-like immunoreactive nerve fibers and calcitonin receptor-like receptor (CLR) expression, and on CGRP receptor (CGRPR) participation in uterine contractility in pigs. E. coli suspension (E. coli group) or saline (SAL group) were injected into the uteri, or only laparotomy was performed (CON group). In the E. coli group myometrium, a lack of significant changes in PGP9.5 and CGRP innervation patterns and increased CLR protein level were revealed. In all groups, compared to the pretreatment period, human αCGRP increased amplitude in the myometrium, while reducing it in endometrium/myometrium. In the E. coli group endometrium/myometrium, human αCGRP lowered amplitude vs other groups. Human αCGRP reduced frequency in CON and SAL groups and enhanced it in the E. coli group endometrium/myometrium. The frequency in E. coli group increased vs other groups. CGRPR antagonist, human αCGRP8-37, reversed (CON, SAL groups) and eliminated (E. coli group) the rise in human αCGRP-induced myometrial amplitude. In endometrium/myometrium, human αCGRP8-37 abolished (CON group) and reversed (SAL group) a decrease in frequency, and reduced the rise in frequency (E. coli group) caused by human αCGRP. Collectively, in the myometrium, endometritis did not change PGP9.5 and CGRP innervation patterns and enhanced CLR protein level. CGRPR also mediated in CGRP action on inflamed uterus contractility.
Topics: Female; Swine; Animals; Humans; Calcitonin Gene-Related Peptide; Endometritis; Escherichia coli; Myometrium; Uterus
PubMed: 36352250
DOI: 10.1038/s41598-022-23867-6 -
Taiwanese Journal of Obstetrics &... Sep 2010
Topics: Female; Humans; Laparoscopy; Leiomyoma; Myometrium; Uterine Neoplasms
PubMed: 21056336
DOI: 10.1016/S1028-4559(10)60086-1 -
Fertility and Sterility Dec 2015
Topics: Adenomyosis; Cell Movement; Endometrium; Epithelial Cells; Female; Humans; Myometrium
PubMed: 26474736
DOI: 10.1016/j.fertnstert.2015.10.005 -
BJOG : An International Journal of... Jan 2014The incidence of placental attachment disorders continues to increase with rising caesarean section rates. Antenatal diagnosis helps in the planning of location, timing... (Review)
Review
The incidence of placental attachment disorders continues to increase with rising caesarean section rates. Antenatal diagnosis helps in the planning of location, timing and staffing of delivery. In at-risk women grey-scale ultrasound is quite sensitive, although colour ultrasound is the most predictive. Magnetic resonance imaging can add information in some limited instances. Patients who have had a previous caesarean section could benefit from early (before 10 weeks) visualisation of the implantation site. Current data refer only to placentas implanted in the lower anterior uterine segment, usually over a caesarean section scar.
Topics: Female; Humans; Imaging, Three-Dimensional; Magnetic Resonance Imaging; Myometrium; Placenta Accreta; Predictive Value of Tests; Pregnancy; Pregnancy Trimesters; Prenatal Diagnosis; Sensitivity and Specificity; Ultrasonography, Doppler, Color
PubMed: 24373591
DOI: 10.1111/1471-0528.12557 -
Progress in Molecular Biology and... 2009Progesterone is a key hormone in the regulation of uterine function. In the normal physiological context, progesterone is primarily involved in remodeling of the... (Review)
Review
Progesterone is a key hormone in the regulation of uterine function. In the normal physiological context, progesterone is primarily involved in remodeling of the endometrium and maintaining a quiescent myometrium. When pathologies of the uterus develop, specifically, endometrial cancer and uterine leiomyoma, response to progesterone is usually altered. Progesterone acts through mainly two isoforms of the progesterone receptor (PR), PRA and PRB which have been reported to exhibit different transcriptional activities. Studies examining the expression and function of the PRs in the normal endometrium and myometrium as well as in endometrial cancer and uterine leiomyoma are summarized here. The clinical use of progestins and the transcriptional activity of the PR on genes specific to endometrial cancer and leiomyoma are described. An increased understanding of the differential expression of PRs and response to progesterone in these two diseases is critical in order to develop more efficient and targeted therapies.
Topics: Endometrial Neoplasms; Endometrium; Female; Humans; Leiomyoma; Myometrium; Receptors, Progesterone
PubMed: 20374701
DOI: 10.1016/S1877-1173(09)87002-6 -
Der Pathologe Mar 2022The present article summarises the recommendations for the handling, histopathological workup, diagnostics and reporting in surgical pathology of biopsies and resection... (Review)
Review
The present article summarises the recommendations for the handling, histopathological workup, diagnostics and reporting in surgical pathology of biopsies and resection specimens in patients with the clinical diagnosis of endometriosis. In addition to practical aspects of pathology, the guidelines also take into account the clinical requirements for histopathology for the optimal diagnosis and therapy of the patients.Based on the definition of endometriosis of the corpus uteri (adenomyosis uteri) most commonly used in the pathological literature, this was defined in the guidelines as the detection of the endometriosis focus in the myometrium at a distance from the endomyometrial border of a medium-sized visual field (100× magnification), which in metric units corresponds to around 2.5 mm. In bowel resection specimens, the status of the resection margins had to be documented within the histopathological report.Also mentioned are the requirements for the reporting of carcinomas associated with endometriosis, including the immunohistochemical evaluation of steroid hormone receptors and mismatch repair proteins.
Topics: Endometriosis; Female; Humans; Myometrium; Uterus
PubMed: 34596734
DOI: 10.1007/s00292-021-00978-x -
Journal of Cellular and Molecular... Dec 2012Small-conductance calcium-activated potassium (SK3) channels have been detected in human myometrium and we have previously shown a functional role of SK channels in...
Small-conductance calcium-activated potassium (SK3) channels have been detected in human myometrium and we have previously shown a functional role of SK channels in human myometrium in vitro. The aims of this study were to identify the precise localization of SK3 channels and to quantify SK3 mRNA expression in myometrium from pregnant and non-pregnant women. Myometrial biopsies were obtained from pregnant (n = 11) and non-pregnant (n = 11) women. The expression of SK3 channels was assessed using immunohistochemistry and SK3 mRNA was determined by qRT-PCR. In non-pregnant myometrium SK3 immunoreactivity was observed in CD34 positive (CD34(+)) interstitial Cajal-like cells (ICLC), now called telocytes. Although CD34(+) cells were also present in pregnant myometrium, they lacked SK3 immunoreactivity. Furthermore, the immunohistochemical results showed that SK3 expression in vascular endothelium was similar between the two groups. CD117 immunoreactivity was only detected in small round cells that resemble mast cells. Compared to non-pregnant myometrium we found significantly less SK3 mRNA in pregnant myometrium. We demonstrate that SK3 channels are localized solely in CD34(+) cells and not in smooth muscle cells, and that the molecular expression of SK3 channels is higher in non-pregnant compared to pregnant myometrium. On the basis of our previous study and the present findings, we propose that SK3 activators reduce contractility in human myometrium by modulating telocyte function. This is the first report to provide evidence for a possible role of SK3 channels in human uterine telocytes.
Topics: Adult; Antigens, CD34; Calcium; Calcium Signaling; Female; Humans; Immunohistochemistry; Interstitial Cells of Cajal; Myocytes, Smooth Muscle; Myometrium; Pregnancy; Proto-Oncogene Proteins c-kit; RNA, Messenger; Small-Conductance Calcium-Activated Potassium Channels; Uterine Contraction
PubMed: 22947283
DOI: 10.1111/j.1582-4934.2012.01627.x -
Molecular Human Reproduction May 2021The onset of labour is a culmination of a series of highly coordinated and preparatory physiological events that take place throughout the gestational period. In order... (Review)
Review
The onset of labour is a culmination of a series of highly coordinated and preparatory physiological events that take place throughout the gestational period. In order to produce the associated contractions needed for foetal delivery, smooth muscle cells in the muscular layer of the uterus (i.e. myometrium) undergo a transition from quiescent to contractile phenotypes. Here, we present the current understanding of the roles transcription factors play in critical labour-associated gene expression changes as part of the molecular mechanistic basis for this transition. Consideration is given to both transcription factors that have been well-studied in a myometrial context, i.e. activator protein 1, progesterone receptors, oestrogen receptors, and nuclear factor kappa B, as well as additional transcription factors whose gestational event-driving contributions have been demonstrated more recently. These transcription factors may form pregnancy- and labour-associated transcriptional regulatory networks in the myometrium to modulate the timing of labour onset. A more thorough understanding of the transcription factor-mediated, labour-promoting regulatory pathways holds promise for the development of new therapeutic treatments that can be used for the prevention of preterm labour in at-risk women.
Topics: Animals; Epigenesis, Genetic; Female; Gene Expression Regulation; Gene Regulatory Networks; Humans; Labor, Obstetric; Myometrium; Parturition; Pregnancy; Transcription Factors; Transcription, Genetic
PubMed: 33823545
DOI: 10.1093/molehr/gaab024 -
BMJ Case Reports Nov 2021The uterine myometrium is the rarest location for an ectopic pregnancy resulting in the so-called ''. It presents a particular diagnostic and therapeutic challenge for...
The uterine myometrium is the rarest location for an ectopic pregnancy resulting in the so-called ''. It presents a particular diagnostic and therapeutic challenge for the treating physician. If passed undiagnosed can lead to life-threatening uterine rupture, which may warrant hysterectomy, leaving the woman with irreversible infertility. Different treatment modalities have been proposed for the management of this condition. In this case report, we are describing a rare case of intramural ectopic pregnancy and reporting the use of hysteroscopy for the surgical management of this case for the first time in the literature.
Topics: Female; Humans; Hysterectomy; Hysteroscopy; Myometrium; Pregnancy; Pregnancy, Ectopic; Uterus
PubMed: 34728506
DOI: 10.1136/bcr-2021-244514 -
BMC Pregnancy and Childbirth Jul 2022Cases of uterine wall thinning and placental abnormalities complicated with systemic lupus erythematosus (SLE) during pregnancy have been reported in Asian countries for... (Review)
Review
BACKGROUND
Cases of uterine wall thinning and placental abnormalities complicated with systemic lupus erythematosus (SLE) during pregnancy have been reported in Asian countries for ten years. Long-term steroid use can cause muscle degeneration, but the mechanism of myometrium thinning was not known. Through the review of published articles, this report is the first review of cases to discuss the pathogenesis and clinical features of thinned myometrium and placenta accreta spectrum (PAS) in pregnant patients with SLE.
CASE PRESENTATION
A twenty-nine-year-old primigravida with a history of lupus enteritis and paralytic ileus had a natural conception after less than two years of steroid treatment. An ultrasonographic study showed a thin uterine wall with a widespread thick placenta on the entire surface of the uterine cavity in the third trimester. At the 39th gestational week, she underwent a cesarean section due to the failure of the uterus to contract, even though the injection of oxytocin. There were several engorged vessels on the surface of the anterior uterine wall at the time of laparotomy. We decided to perform a hysterectomy because diffuse PAS replaced her uterus.
CONCLUSION
A review of reported cases and our case shows an unusual complication of SLE that might be related to the particular condition of the estrogen-mediated immune system. Clinicians should always pay attention to the possibility of uterine wall thinning as uterine atony and the structural abnormality of the placenta for SLE patients with the unscarred uterus.
Topics: Adult; Cesarean Section; Female; Humans; Lupus Erythematosus, Systemic; Myometrium; Placenta; Placenta Accreta; Pregnancy; Steroids
PubMed: 35780086
DOI: 10.1186/s12884-022-04864-z