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Veterinary World Jul 2022Pomegranate is known to possess antibacterial properties, partly because of its punicalagin content. However, its effect on canine oral bacterial species has not yet...
BACKGROUND AND AIM
Pomegranate is known to possess antibacterial properties, partly because of its punicalagin content. However, its effect on canine oral bacterial species has not yet been elucidated. In this study, we evaluated the effect of pomegranate extract present in pet dental products on the growth and survival of five canine oral bacterial species in biofilms.
MATERIALS AND METHODS
Five bacterial species, , , , , and , were individually cultured for biofilm formation and exposed to pomegranate extract (or control) for 15 min. Cell survival was analyzed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and was compared between different conditions using a student's t-test. In addition, the individual strains were grown in planktonic suspensions and exposed to serial dilutions of the extract to determine the minimum inhibitory concentration.
RESULTS
At a concentration of 0.035% w/v, the extract significantly reduced the survival of (-39%, p < 0.001) and (-28%, p = 0.08) in biofilms. At similar concentrations, the extract also completely or partially inhibited the growth of spp. in planktonic suspensions, respectively.
CONCLUSION
The pomegranate extract found in some pet dental products can limit bacterial growth and survival in the biofilms formed by and . As is involved in periodontal disease progression, limiting its proliferation using products containing pomegranate extract could contribute to disease prevention. Further studies on dogs receiving such products are necessary to confirm these effects.
PubMed: 36185506
DOI: 10.14202/vetworld.2022.1714-1718 -
Cureus Jan 2024is a common bacteria that colonizes in humans. Of the 11 species, only two, and are pathogenic. Although sparse, there are case reports of other Neisseria species...
is a common bacteria that colonizes in humans. Of the 11 species, only two, and are pathogenic. Although sparse, there are case reports of other Neisseria species causing infections in humans. , which is a part of normal flora in the mouths of dogs and cats, has been shown to have potential to be pathogenic in humans. The standard treatment for dog and cat bites is oral amoxicillin/clavulanic acid (Augmentin) or IV ampicillin/sulbactam (Unasyn). However, in cases where the patient has multiple antibiotic allergies, careful antibiotic selection must be made to ensure resolution of infection.
PubMed: 38384600
DOI: 10.7759/cureus.52712 -
Antonie Van Leeuwenhoek Jul 2019Two Gram-stain negative, catalase positive, coccus shaped bacteria, designated 10023 and 10010, were isolated from the rectal contents of a plateau pika (Ochotona...
Two Gram-stain negative, catalase positive, coccus shaped bacteria, designated 10023 and 10010, were isolated from the rectal contents of a plateau pika (Ochotona curzoniae) in Qinghai-Tibet Plateau, China. Based on 16S rRNA gene sequence analysis, phylogenetic trees showed that these two isolates (10023, 10010) group with members of the genus Neisseria. Additionally, these two isolates exhibited high 16S rRNA gene sequence similarity with Neisseria zalophi CSL 7565 (96.98%), Neisseria wadsworthii WC 05-9715 (96.92%) and Neisseria canis ATCC 14687 (96.79%). Further phylogenetic analysis based on the rplF gene showed that these two novel strains can be easily discriminated from phylogenetically closely related species. Optimal growth was found to occur on BHI agar with 5% defibrinated sheep blood at 37 °C and growth was also observed on nutrient agar, Columbia blood agar and chocolate agar plates; however, growth was not observed on MacConkey agar after 7 days. The major cellular fatty acids of these strains were identified as C and Cω7c/Cω6c. The complete genome size of the type strain 10023 is 2,496,444 bp, with DNA G+C content of 54.0 mol %. The average nucleotide identity values were 73.5-79.3% between isolate 10023 and reference Neisseria spp. Based on polyphasic analysis, these isolates (10023 and 10010) are considered to represent a novel species in the genus Neisseria, for which the name Neisseria chenwenguii sp. nov. is proposed. The type strain is 10023 (= DSM 103440 = CGMCC 1.15736).
Topics: Animals; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Lagomorpha; Neisseria; Phylogeny; RNA, Ribosomal, 16S; Rectum; Tibet
PubMed: 30798492
DOI: 10.1007/s10482-019-01234-2 -
International Journal of Systematic and... May 2017During the summers of 2013 and 2014, isolates of a novel Gram-stain-negative coccus in the genus Neisseriawere obtained from the contents of nonviable greater...
During the summers of 2013 and 2014, isolates of a novel Gram-stain-negative coccus in the genus Neisseriawere obtained from the contents of nonviable greater white-fronted goose (Anseralbifrons) eggs on the Arctic Coastal Plain of Alaska. We used a polyphasic approach to determine whether these isolates represent a novel species. 16S rRNA gene sequences, 23S rRNA gene sequences, and chaperonin 60 gene sequences suggested that these Alaskan isolates are members of a distinct species that is most closely related to Neisseria canis, Neisseriaanimaloris and Neisseriashayeganii. Analysis of the rplF gene additionally showed that the isolates are unique and most closely related to Neisseriaweaveri. Average nucleotide identity of the whole genome sequence of the type strain was between 71.5 and 74.6 % compared to close relatives, further supporting designation as a novel species. Fatty acid methyl ester analysis showed a predominance of C14 : 0, C16 : 0 and C16 : 1ω7c fatty acids. Finally, biochemical characteristics distinguished the isolates from other species of the genus Neisseria. On the basis of these combined data, the isolates are proposed to represent a novel species of the genus Neisseria, with the name Neisseria arctica sp. nov. The type strain is KH1503T (=ATCC TSD-57T=DSM 103136T).
Topics: Alaska; Animals; Arctic Regions; Bacterial Typing Techniques; Base Composition; Chaperonin 60; DNA, Bacterial; Fatty Acids; Geese; Neisseria; Ovum; Phylogeny; RNA, Ribosomal, 16S; RNA, Ribosomal, 23S; Sequence Analysis, DNA
PubMed: 28056218
DOI: 10.1099/ijsem.0.001773 -
Journal of Clinical Microbiology Sep 1993CDC group M-5 is a rod-shaped, gram-negative, nonmotile bacterium associated with dog bite wounds. DNA-DNA relatedness and biochemical and growth characteristics were...
CDC group M-5 is a rod-shaped, gram-negative, nonmotile bacterium associated with dog bite wounds. DNA-DNA relatedness and biochemical and growth characteristics were studied for 54 strains from the collection at the Centers for Disease Control and Prevention. One typical M-5 strain, 8142, was further studied by 16S rRNA sequencing. DNA from 40 of 53 strains showed 82 to 100% relatedness (hydroxyapatite method) to labeled DNA from strain 8142. The guanine-plus-cytosine (G + C) content in 8 of the 41 highly related M-5 strains was 50.5 to 52 mol%. These 41 strains were oxidase and catalase positive, nonfermentative, nitrite positive, nitrate negative, weakly phenylalanine deaminase positive, aerobic, and alpha-hemolytic (sheep blood). DNA from the 13 remaining strains showed only 7 to 46% DNA relatedness to strain 8142. These 13 non-M-5 strains differed from the M-5 strains in G + C content, growth characteristics, and biochemical profiles. DNA from M-5 strain 8142 was most closely related to DNA from groups EF-4b (47%) and EF-4a (45%). 16S rRNA sequence analysis placed M-5 strain 8142 in the Neisseriaceae cluster of the beta-3 subgroup of the class Proteobacteria. It was most homologous (98.4 to 98.8%) to Neisseria animalis, Neisseria flavescens, Neisseria canis, and Neisseria elongata. All data are consistent with M-5 being a new species of Neisseria, for which we propose the name Neisseria weaveri.
Topics: Animals; Base Composition; Base Sequence; Bites and Stings; DNA, Bacterial; Dogs; Molecular Sequence Data; Neisseria; Neisseriaceae Infections; Nucleic Acid Hybridization; RNA, Ribosomal, 16S; Wound Infection
PubMed: 8408570
DOI: 10.1128/jcm.31.9.2456-2466.1993 -
Journal of Clinical Microbiology Aug 2005Pasteurella dagmatis and Neisseria canis were repeatedly isolated from the sputum of a poodle-owning patient with chronic bronchiectasis. Commercially available systems...
Pasteurella dagmatis and Neisseria canis were repeatedly isolated from the sputum of a poodle-owning patient with chronic bronchiectasis. Commercially available systems failed to identify these unusual organisms: identification was made by 16S rRNA gene sequencing. Difficulties identifying these and five other canine-associated isolates (P. dagmatis [n = 2], Pasteurella canis [n = 2], and N. canis [n = 1]) are discussed.
Topics: Aged; Animals; Bronchiectasis; Chronic Disease; Dogs; Humans; Male; Neisseria; Pasteurella; RNA, Ribosomal, 16S; Respiratory Tract Infections
PubMed: 16081998
DOI: 10.1128/JCM.43.8.4272-4274.2005 -
Communicable Diseases Intelligence Aug 1999The third case report, which is the first in Australia, of human infection with Neisseria canis is documented. This is the first case report in which the pathogenicity...
The third case report, which is the first in Australia, of human infection with Neisseria canis is documented. This is the first case report in which the pathogenicity of this organism for humans is unequivocally demonstrated.
Topics: Animals; Diagnosis, Differential; Dogs; Foot Injuries; Humans; Male; Middle Aged; Neisseria; Neisseriaceae Infections; Wound Infection; Wounds, Penetrating
PubMed: 10497835
DOI: No ID Found -
Journal of Clinical Microbiology Jul 1989We report a case of identification of Neisseria canis in a human patient after a cat bite. This organism was isolated from a mixed culture together with Pasteurella...
We report a case of identification of Neisseria canis in a human patient after a cat bite. This organism was isolated from a mixed culture together with Pasteurella multocida and Eikenella corrodens. It is the second case report of a human infection.
Topics: Adult; Animals; Bites and Stings; Cats; Female; Humans; Neisseria; Wound Infection
PubMed: 2768456
DOI: 10.1128/jcm.27.7.1673-1674.1989 -
Journal of Clinical Microbiology May 2011Neisseria canis was isolated in pure culture from a mandibular abscess in a dog. Ultrasound-guided fine-needle aspiration was used to obtain a sample from the abscess....
Neisseria canis was isolated in pure culture from a mandibular abscess in a dog. Ultrasound-guided fine-needle aspiration was used to obtain a sample from the abscess. Conventional bacteriological examination techniques followed by 16S rRNA gene sequencing from pure subculture and construction of a phylogenetic tree verified the isolate as N. canis. 16S rRNA sequence analysis revealed that a broader phylogenetic platform is needed in the part of the phylogenetic tree where the canine pathogenic N. canis isolate is located. The canine pathogenic isolate was found to be resistant to cephalexin and trimethoprim.
Topics: Abscess; Animals; Biopsy, Fine-Needle; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Dog Diseases; Dogs; Face; Gram-Negative Bacterial Infections; Mandibular Diseases; Neisseria; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Wound Infection
PubMed: 21411579
DOI: 10.1128/JCM.02610-10