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The Journal of Biological Chemistry Feb 1957
Topics: Ergothioneine; Neurospora
PubMed: 13405916
DOI: No ID Found -
Proceedings of the National Academy of... Jun 1993
Review
Topics: Animals; Biological Evolution; Circadian Rhythm; Drosophila melanogaster; Genes, Fungal; Neurospora; Oscillometry; RNA, Messenger
PubMed: 8516281
DOI: 10.1073/pnas.90.12.5382 -
Genes & Development Feb 2004Phosphorylation of the Neurospora circadian clock protein FREQUENCY by several kinases promotes its degradation and is important for the function of the circadian...
Phosphorylation of the Neurospora circadian clock protein FREQUENCY by several kinases promotes its degradation and is important for the function of the circadian feedback loop. Here, we show that FRQ is less stable in a ppp-1 (catalytic subunit of PP1) mutant, resulting in its advanced phase and short period. In contrast, FRQ stability is not altered in a rgb-1 (a regulatory subunit of PP2A) mutant, but levels of frq protein and mRNA are low, resulting in a low-amplitude and long-period oscillation of the clock. Furthermore, PP1 and PP2A expressed in Neurospora can dephosphorylate the endogenous FRQ in vitro, suggesting that these two phosphatases may differentially regulate FRQ and, consequently, the behavior of the circadian clock.
Topics: Catalytic Domain; Circadian Rhythm; Fungal Proteins; Mutation; Neurospora; Phosphorylation
PubMed: 14871927
DOI: 10.1101/gad.1152604 -
FEBS Letters Aug 2022Timing by the circadian clock of Neurospora is associated with hyperphosphorylation of frequency (FRQ), which depends on anchoring casein kinase 1a (CK1a) to FRQ. It is...
Timing by the circadian clock of Neurospora is associated with hyperphosphorylation of frequency (FRQ), which depends on anchoring casein kinase 1a (CK1a) to FRQ. It is not known how CK1a is anchored so that approximately 100 sites in FRQ can be targeted. Here, we identified two regions in CK1a, p1 and p2, that are required for anchoring to FRQ. Mutation of p1 or p2 impairs progressive hyperphosphorylation of FRQ. A p1-mutated strain is viable but its circadian clock is non-functional, whereas a p2-mutated strain is non-viable. Our data suggest that p1 and potentially also p2 in CK1a provide an interface for interaction with FRQ. Anchoring via p1-p2 leaves the active site of CK1a accessible for phosphorylation of FRQ at multiple sites.
Topics: Casein Kinases; Circadian Clocks; Circadian Rhythm; Fungal Proteins; Neurospora; Neurospora crassa
PubMed: 35735764
DOI: 10.1002/1873-3468.14435 -
The Journal of Biological Chemistry Sep 2006
Review
Topics: Circadian Rhythm; Fungal Proteins; Gene Expression Regulation, Fungal; Genes, Fungal; Models, Biological; Neurospora
PubMed: 16905532
DOI: 10.1074/jbc.R600018200 -
Microbiology (Reading, England) Jul 1998
Review
Topics: Biological Clocks; Circadian Rhythm; Fungal Proteins; Genes, Fungal; Light; Neurospora
PubMed: 9695904
DOI: 10.1099/00221287-144-7-1699 -
Microbiological Reviews Mar 1981
Review
Topics: Amino Acids; Cell Cycle; DNA, Fungal; Escherichia coli; Fungal Proteins; Mathematics; Models, Biological; Neurospora; Neurospora crassa; RNA, Fungal; Ribosomes; Temperature
PubMed: 6452573
DOI: 10.1128/mr.45.1.99-122.1981 -
The Journal of Biological Chemistry Feb 1980
Topics: Base Sequence; Cell Nucleus; DNA, Fungal; Genes, Regulator; Kinetics; Molecular Weight; Neurospora; Neurospora crassa; Nucleic Acid Conformation; Nucleic Acid Renaturation; RNA, Fungal; Ribosomes
PubMed: 6444301
DOI: No ID Found -
Journal of Evolutionary Biology Aug 2010Maladaptive hybridization promotes reinforcement, selection for stringent reproductive isolation barriers during speciation. Reinforcement is suspected when barriers...
Maladaptive hybridization promotes reinforcement, selection for stringent reproductive isolation barriers during speciation. Reinforcement is suspected when barriers between sympatric populations are stronger than allopatric barriers, and particularly when stronger barriers evolve in the species and sex suffering the greatest costs of hybridization. Canonically, reinforcement involves premating barriers. Selection for postmating barriers is controversial, but theoretically possible. We examined geographical patterns in reproductive isolation barriers between Neurospora crassa and Neurospora intermedia, fungi with pheromone-mediated mate recognition and maternal care. We find that isolation is stronger between sympatric populations than allopatric populations, and stronger barriers are associated with the species (N. crassa) and mating role (maternal) suffering the greater costs of hybridization. Notably, reinforced isolation involves a postmating barrier, abortion of fruitbodies. We hypothesize that fruitbody abortion is selectively advantageous if it increases the likelihood that maternal Neurospora individuals successfully mate conspecifically after maladaptive hybrid fertilization.
Topics: Fruiting Bodies, Fungal; Genetic Speciation; Geography; Hybridization, Genetic; Neurospora; Neurospora crassa; Reproduction
PubMed: 20546092
DOI: 10.1111/j.1420-9101.2010.02030.x -
The Journal of Cell Biology Oct 1971A paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type Neurospora crassa after growth in either...
A paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type Neurospora crassa after growth in either ethidium or euflavine. This paracrystal has been isolated and partially characterized. It appears to be composed of a single polypeptide (mol wt 68,000) which can be reversibly crystallized and dissociated by changes in the pH and ionic strength. When aggregated, the polypeptide forms oligomers which are arranged end-to-end into fibers. During the characterization of the polypeptide, it was found that the polypeptide's electrophoretic and immunological properties could be used as assays. Using these methods it was found that the polypeptide normally accumulates in a soluble form in the cytoplasm of wild-type Neurospora at the end of the log-phase of growth.
Topics: Acridines; Animals; Antibody Formation; Buffers; Carbon Isotopes; Cell Fractionation; Crystallization; Cytochromes; Cytoplasm; Electrophoresis, Disc; Histocytochemistry; Hydrogen-Ion Concentration; Immunodiffusion; Immunoelectrophoresis; Inclusion Bodies; Microscopy, Electron; Microscopy, Phase-Contrast; Mitochondria; Molecular Weight; Neurospora; Peptides; Phenanthridines; Protein Biosynthesis; Rabbits; Sulfur Isotopes; Time Factors; Tritium
PubMed: 4329522
DOI: 10.1083/jcb.51.1.249