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Genomics Data Mar 2017SUBG007 was isolated from the fruit of in Rajkot (22.30°N, 70.78°E), Gujarat, India. Here we present the 4.37 Mb genome sequence strain SUBG007, which may provide...
SUBG007 was isolated from the fruit of in Rajkot (22.30°N, 70.78°E), Gujarat, India. Here we present the 4.37 Mb genome sequence strain SUBG007, which may provide the genetic information for the application in environment pollution degradation and agriculture field. The strain also posses many genes cluster which involved in production of important secondary metabolites. The nucleotide sequence of this genome was deposited into NCBI GenBank under the accession LUAY00000000.
PubMed: 28119820
DOI: 10.1016/j.gdata.2017.01.001 -
BMC Microbiology Mar 2014Ochrobactrum anthropi (O. anthropi), is a non-fermenting gram-negative bacillus usually found in the environment. Nevertheless, during the past decade it has been...
Typing of Ochrobactrum anthropi clinical isolates using automated repetitive extragenic palindromic-polymerase chain reaction DNA fingerprinting and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
BACKGROUND
Ochrobactrum anthropi (O. anthropi), is a non-fermenting gram-negative bacillus usually found in the environment. Nevertheless, during the past decade it has been identified as pathogenic to immunocompromised patients. In this study, we assessed the usefulness of the automated repetitive extragenic palindromic-polymerase chain reaction (rep-PCR-based DiversiLab™ system, bioMèrieux, France) and of matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF MS) for typing of twentythree O. anthropi clinical isolates that we found over a four-months period (from April 2011 to August 2011) in bacteriemic patients admitted in the same operative unit of our hospital. Pulsed-field gel electrophoresis (PFGE), commonly accepted as the gold standard technique for typing, was also used. Analysis was carried out using the Pearson correlation coefficient to determine the distance matrice and the unweighted pair group method with arithmetic mean (UPGMA) to generate dendogram.
RESULTS
Rep-PCR analysis identified four different patterns: three that clustered together with 97% or more pattern similarity, and one whose members showed < 95% pattern similarity. Interestingly, strains isolated later (from 11/06/2011 to 24/08/2011) displayed a pattern with 99% similarity. MALDI-TOF MS evaluation clustered the twentythree strains of O. anthropi into a single group containing four distinct subgroups, each comprising the majority of strains clustering below 5 distance levels, indicating a high similarity between the isolates.
CONCLUSIONS
Our results indicate that these isolates are clonally-related and the methods used afforded a valuable contribution to the epidemiology, prevention and control of the infections caused by this pathogen.
Topics: Bacteremia; Bacterial Typing Techniques; Cluster Analysis; DNA Fingerprinting; Electrophoresis, Gel, Pulsed-Field; France; Genotype; Gram-Negative Bacterial Infections; Hospitals; Humans; Molecular Epidemiology; Ochrobactrum anthropi; Polymerase Chain Reaction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 24655432
DOI: 10.1186/1471-2180-14-74 -
Journal of Applied Microbiology Feb 2010Hypersensitivity pneumonitis of machinists associated with metalworking fluids (MWF) was recently linked to Mycobacterium immunogenum. In addition to Mycobacterium,...
AIMS
Hypersensitivity pneumonitis of machinists associated with metalworking fluids (MWF) was recently linked to Mycobacterium immunogenum. In addition to Mycobacterium, impacts of continuous and massive contact to other micro-organisms, such as Pseudomonas, were little studied. This report intended to quantify and characterize the microbial load of 44 in-use MWF.
METHODS AND RESULTS
The main biodiversity of MWF was assessed using cultural methods, quantitative PCR (qPCR) and denaturing gradient gel electrophoresis (DGGE). Total bacteria concentrations ranged from undetectable to 10(9) 16S rRNA gene copies per millilitre. Concentrations obtained by qPCR were up to five orders of magnitude higher than by culture, suggesting that MWF contamination is generally underestimated. Two samples showed high concentrations of Myco. immunogenum (1.55 x 10(7) and 3.49 x 10(5) 16S rRNA gene copies per millilitre). The overall biodiversity was low, as observed by culture and DGGE, and was comparable to data found in the literature. Pseudomonas pseudoalcaligenes was by far the main bacteria found in MWF samples (33 out of 44), followed by Ochrobactrum anthropi (32 out of 44). There was no significant relationship between the biodiversity profiles and the kind of MWF or equipment used, making it difficult to predict which micro-organisms will colonize each particular MWF.
CONCLUSIONS
Very high concentrations of bacteria were found in most MWF studied and limited biodiversities were observed. Many species of micro-organisms were retrieved from MWF samples, but they were mostly colonized by Pseudomonas pseudoalcaligenes and Ochrobactrum anthropi.
SIGNIFICANCE AND IMPACT OF THE STUDY
The major micro-organisms observed or recovered in this study from in-use MWF were present in very high concentrations, and thus further studies are needed to confirm their role in workers' respiratory disorders or health-related problems.
Topics: Biodiversity; Equipment Contamination; Metallurgy; Mycobacterium; Ochrobactrum anthropi; Polymerase Chain Reaction; Pseudomonas pseudoalcaligenes; RNA, Bacterial; RNA, Ribosomal, 16S
PubMed: 19614850
DOI: 10.1111/j.1365-2672.2009.04433.x -
Scientific Reports Apr 2023In a survey conducted during the period of March-May 2019 in nurseries, warehouses, and shops at three governorates (Alexandria, El-Behera, and Giza governorates,...
In a survey conducted during the period of March-May 2019 in nurseries, warehouses, and shops at three governorates (Alexandria, El-Behera, and Giza governorates, Egypt), symptoms of root rot, basal stem rot, and wilt disease complex were observed in the lucky bamboo (Dracaena sanderiana hort. ex. Mast.). The highest disease infection percentage was found in lucky bamboo collected from Alexandria City (47.67%), while the highest disease severity was in lucky bamboo collected from El-Behera Governorate (35.19%). Rhizoctonia solani, Fusarium oxysporum, F. solani, Aspergillus niger, and Alternaria alternate were isolated and identified in the infected lucky bamboo samples. R. solani isolates were the most dominant among the recovered fungal species with a percentage of 80.89% of the total isolates (246). Pathogenicity tests showed that R. solani was the most pathogen with 100% disease infection and 76.67% disease severity. Molecular identification characterized R. solani isolate as R. solani AUMC 15120, MZ723906. Meanwhile, four biological control agents (bioagents) were isolated from the healthy lucky bamboo samples and identified based on cultural, morphological, microscopic characteristics, and the molecular phylogenetic analysis as Clonostachys rosea AUMC 15121, OL461708; Bacillus circulans TAG1, MW441316; B. siamensis TAP1, MW441318 and Ochrobactrum anthropi TAM1, MW441317. The four bioagents showed potential inhibition of R. solani in vitro as well as in vivo on lucky bamboo plants in vase treatments compared to the untreated inoculated control as well as certain fungicides and biocides used (Moncut, Rizolex-T, Topsin-M, Bio-Zeid, and Bio-Arc). The bioagent O. anthropi showed the highest inhibition growth (85.11%) of the in vitro R. solani colony, which was not significantly different from the biocide Bio-Arc (83.78%). However, C. rosea, B. siamensis and B. circulans showed inhibition values of 65.33, 64.44, and 60.44%, respectively. On the other hand, the biocide Bio-Zeid showed less inhibitory effect (43.11%), while the lowest growth inhibition was recorded by Rizolex-T (34.22%) and Topsin-M (28.67%). Furthermore, the in vivo experiment supported the in vitro results for the most effective treatments, where all the treatments significantly decreased the percentage of infection and disease severity compared to the inoculated untreated control. Additionally, the bioagent O. anthropi showed the highest effect, i.e., the lowest disease incidence and disease severity being 13.33% and 10%, compared to 100% and 75%, respectively, in the untreated inoculated control. This was not significantly different from the fungicide Moncut (13.33% and 21%) and from the bioagent C. rosea (20% and 15%) treatments for both parameters, respectively. In conclusion, the bioagents O. anthropi MW441317 at 1 × 10 CFU/ml as well as C. rosea AUMC15121 at 1 × 10/ml proved to be efficient to control R. solani causing root rot, and basal stem rot on lucky bamboo, compared to fungicide Moncut and can be used for disease management without the negative impact of the chemical control. Furthermore, this is the first report of the isolation and identification of Rhizoctonia solani, a pathogenic fungus, and four biocontrol agents (Bacillus circulans, B. siamensis, Ochrobactrum anthropi and Clonostachys rosea) associated with the healthy lucky bamboo plants.
Topics: Dracaena; Phylogeny; Fungicides, Industrial
PubMed: 37095150
DOI: 10.1038/s41598-023-33628-8 -
Infection and Immunity Jun 2000The outer membrane (OM) of the intracellular parasite Brucella abortus is permeable to hydrophobic probes and resistant to destabilization by polycationic peptides and... (Comparative Study)
Comparative Study
The outer membrane (OM) of the intracellular parasite Brucella abortus is permeable to hydrophobic probes and resistant to destabilization by polycationic peptides and EDTA. The significance of these unusual properties was investigated in a comparative study with the opportunistic pathogens of the genus Ochrobactrum, the closest known Brucella relative. Ochrobactrum spp. OMs were impermeable to hydrophobic probes and sensitive to polymyxin B but resistant to EDTA. These properties were traced to lipopolysaccharide (LPS) because (i) insertion of B. abortus LPS, but not of Escherichia coli LPS, into Ochrobactrum OM increased its permeability; (ii) permeability and polymyxin B binding measured with LPS aggregates paralleled the results with live bacteria; and (iii) the predicted intermediate results were obtained with B. abortus-Ochrobactrum anthropi and E. coli-O. anthropi LPS hybrid aggregates. Although Ochrobactrum was sensitive to polymyxin, self-promoted uptake and bacterial lysis occurred without OM morphological changes, suggesting an unusual OM structural rigidity. Ochrobactrum and B. abortus LPSs showed no differences in phosphate, qualitative fatty acid composition, or acyl chain fluidity. However, Ochrobactrum LPS, but not B. abortus LPS, contained galacturonic acid. B. abortus and Ochrobactrum smooth LPS aggregates had similar size and zeta potential (-12 to -15 mV). Upon saturation with polymyxin, zeta potential became positive (1 mV) for Ochrobactrum smooth LPS while remaining negative (-5 mV) for B. abortus smooth LPS, suggesting hindered access to inner targets. These results show that although Ochrobactrum and Brucella share a basic OM pattern, subtle modifications in LPS core cause markedly different OM properties, possibly reflecting the adaptive evolution of B. abortus to pathogenicity.
Topics: Alphaproteobacteria; Brucella abortus; Cations; Cell Membrane; Cell Membrane Permeability; Drug Resistance, Microbial; Gram-Negative Aerobic Rods and Cocci; Lipopolysaccharides; Membrane Potentials; Ochrobactrum anthropi; Peptides; Polylysine; Polymyxin B
PubMed: 10816465
DOI: 10.1128/IAI.68.6.3210-3218.2000 -
Marine Drugs Feb 2022Epibiotic bacteria associated with the filamentous marine cyanobacterium were explored as a novel source of antibiotics and to establish whether they can produce...
Epibiotic bacteria associated with the filamentous marine cyanobacterium were explored as a novel source of antibiotics and to establish whether they can produce cyclodepsipeptides on their own. Here, we report the isolation of micrococcin P1 () (CHNOS; obs. / 1144.21930/572.60381) and micrococcin P2 () (CHNOS; obs. / 1142.20446/571.60370) from a strain of isolated from ' filaments. Interestingly, most bacteria isolated from ' filaments were found to be human pathogens. Stalked diatoms on the filaments suggested a possible terrestrial origin of some epibionts. CuSO·5HO assisted differential genomic DNA isolation and phylogenetic analysis showed that a Kenyan strain of differed from strain CCAP 1446/4 and clones. Organic extracts of the epibiotic bacteria and did not produce cyclodepsipeptides. Further characterization of 24 Firmicutes strains from identified extracts of as most active. Our results showed that the genetic basis for synthesizing micrococcin P1 (), discovered in ATCC 14579, is species/strain-dependent and this reinforces the need for molecular identification of species worldwide and their epibionts. These findings indicate that -associated bacteria are an overlooked source of antimicrobial compounds.
Topics: Anti-Infective Agents; Bacillus; Bacteriocins; Cyanobacteria; Depsipeptides; Kenya; Phylogeny; Species Specificity
PubMed: 35200657
DOI: 10.3390/md20020128 -
Applied and Environmental Microbiology Apr 2001A bacterium, Ochrobactrum anthropi, produced a large amount of a nucleosidase when cultivated with purine nucleosides. The nucleosidase was purified to homogeneity. The...
A bacterium, Ochrobactrum anthropi, produced a large amount of a nucleosidase when cultivated with purine nucleosides. The nucleosidase was purified to homogeneity. The enzyme has a molecular weight of about 170,000 and consists of four identical subunits. It specifically catalyzes the irreversible N-riboside hydrolysis of purine nucleosides, the K(m) values being 11.8 to 56.3 microM. The optimal activity temperature and pH were 50 degrees C and pH 4.5 to 6.5, respectively. Pyrimidine nucleosides, purine and pyrimidine nucleotides, NAD, NADP, and nicotinamide mononucleotide are not hydrolyzed by the enzyme. The purine nucleoside hydrolyzing activity of the enzyme was inhibited (mixed inhibition) by pyrimidine nucleosides, with K(i) and K(i)' values of 0.455 to 11.2 microM. Metal ion chelators inhibited activity, and the addition of Zn(2+) or Co(2+) restored activity. A 1.5-kb DNA fragment, which contains the open reading frame encoding the nucleosidase, was cloned, sequenced, and expressed in Escherichia coli. The deduced 363-amino-acid sequence including a 22-residue leader peptide is in agreement with the enzyme molecular mass and the amino acid sequences of NH(2)-terminal and internal peptides, and the enzyme is homologous to known nucleosidases from protozoan parasites. The amino acid residues forming the catalytic site and involved in binding with metal ions are well conserved in these nucleosidases.
Topics: Amino Acid Sequence; Cloning, Molecular; DNA, Bacterial; Escherichia coli; Gene Expression Regulation, Bacterial; Kinetics; Molecular Sequence Data; N-Glycosyl Hydrolases; Ochrobactrum anthropi; Pyrimidine Nucleosides; Sequence Analysis, DNA; Substrate Specificity
PubMed: 11282633
DOI: 10.1128/AEM.67.4.1783-1787.2001 -
Clinical Microbiology and Infection :... Sep 1999
PubMed: 11851711
DOI: 10.1111/j.1469-0691.1999.tb00437.x -
Frontiers in Cellular and Infection... 2016are highly infectious bacterial pathogens responsible for brucellosis, a frequent worldwide zoonosis. The genus has recently expanded from 6 to 11 species, all of...
are highly infectious bacterial pathogens responsible for brucellosis, a frequent worldwide zoonosis. The genus has recently expanded from 6 to 11 species, all of which were associated with mammals; The natural host range recently expanded to amphibians after some reports of atypical strains from frogs. Here we describe the first in depth phenotypic and genetic characterization of a strains isolated from a frog. Strain B13-0095 was isolated from a Pac-Man frog () at a veterinary hospital in Texas and was initially misidentified as . We found that B13-0095 belongs to a group of early-diverging brucellae that includes strain BO1 and the -like strain BO2, with traits that depart significantly from those of the "classical" spp. Analysis of B13-0095 genome sequence revealed several specific features that suggest that this isolate represents an intermediate between a soil associated ancestor and the host adapted "classical" species. Like strain BO2, B13-0095 does not possess the genes required to produce the perosamine based LPS found in classical , but has a set of genes that could encode a rhamnose based O-antigen. Despite this, B13-0095 has a very fast intracellular replication rate in both epithelial cells and macrophages. Finally, another major finding in this study is the bacterial motility observed for strains B13-0095, BO1, and BO2, which is remarkable for this bacterial genus. This study thus highlights several novel characteristics in strains belonging to an emerging group within the genus. Accurate identification tools for such atypical isolates and careful evaluation of their zoonotic potential, are urgently required.
Topics: Animals; Anura; Bacterial Proteins; Base Sequence; Biological Evolution; Brucella; Brucellosis; Carbon; Cell Line; Child; DNA, Bacterial; Epithelial Cells; Female; Genes, Bacterial; Genome, Bacterial; HeLa Cells; Humans; Lipopolysaccharides; Macrophages; Mice; Multigene Family; O Antigens; Phenotype; Phylogeny; Rhamnose; Texas; Virulence; Zoonoses
PubMed: 27734009
DOI: 10.3389/fcimb.2016.00116 -
Journal of Medical Case Reports Dec 2008Cystic fibrosis afflicted lungs support the growth of many bacteria rarely implicated in other cases of human infections.
INTRODUCTION
Cystic fibrosis afflicted lungs support the growth of many bacteria rarely implicated in other cases of human infections.
CASE PRESENTATION
We report the isolation and identification, by 16S rRNA amplification and sequencing, of two emerging pathogens resistant to colistin, Brevundimonas diminuta and Ochrobactrum anthropi, in a 17-year-old woman with cystic fibrosis and pneumonia. The patient eventually responded well to a 2-week regime of imipenem and tobramycin.
CONCLUSION
Our results clearly re-emphasize the emergence of new colistin-resistant pathogens in patients with cystic fibrosis.
PubMed: 19061488
DOI: 10.1186/1752-1947-2-373