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Sensors (Basel, Switzerland) Sep 2018Odour perception has been the object of fast growing research interest in the last three decades. Parallel to the study of the corresponding biological systems, attempts... (Review)
Review
Odour perception has been the object of fast growing research interest in the last three decades. Parallel to the study of the corresponding biological systems, attempts are being made to model the olfactory system with electronic devices. Such projects range from the fabrication of individual sensors, tuned to specific chemicals of interest, to the design of multipurpose smell detectors using arrays of sensors assembled in a sort of artificial nose. Recently, proteins have attracted increasing interest as sensing elements. In particular, soluble olfaction proteins, including odorant-binding proteins (OBPs) of vertebrates and insects, chemosensory proteins (CSPs) and Niemann-Pick type C2 (NPC2) proteins possess interesting characteristics for their use in sensing devices for odours. In fact, thanks to their compact structure, their soluble nature and small size, they are extremely stable to high temperature, refractory to proteolysis and resistant to organic solvents. Moreover, thanks to the availability of many structures solved both as apo-proteins and in complexes with some ligands, it is feasible to design mutants by replacing residues in the binding sites with the aim of synthesising proteins with better selectivity and improved physical properties, as demonstrated in a number of cases.
Topics: Animals; Biosensing Techniques; Electronic Nose; Humans; Odorants; Receptors, Odorant
PubMed: 30262737
DOI: 10.3390/s18103248 -
Current Environmental Health Reports Sep 2023Organosulfur compounds are intentionally added to natural gas as malodorants with the intent of short-term nasal inhalation to aid in leak detection. Regulatory exposure... (Review)
Review
PURPOSE OF REVIEW
Organosulfur compounds are intentionally added to natural gas as malodorants with the intent of short-term nasal inhalation to aid in leak detection. Regulatory exposure limits have not been established for all commonly used natural gas odorants, and recent community-level exposure events and growing evidence of indoor natural gas leakage have raised concerns associated with natural gas odorant exposures. We conducted a scoping review of peer-reviewed scientific publications on human exposures and animal toxicological studies of natural gas odorants to assess toxicological profiles, exposure potential, health effects and regulatory guidelines associated with commonly used natural gas odorants.
RECENT FINDINGS
We identified only 22 studies which met inclusion criteria for full review. Overall, there is limited evidence of both transient nonspecific health symptoms and clinically diagnosed causative neurotoxic effects associated with prolonged odorant exposures. Across seven community-level exposure events and two occupational case reports, consistent symptom patterns included: headache, ocular irritation, nose and throat irritation, respiratory complaints such as shortness of breath and asthma attacks, and skin irritation and rash. Of these, respiratory inflammation and asthma exacerbations are the most debilitating, whereas the high prevalence of ocular and dermatologic symptoms suggest a non-inhalation route of exposure. The limited evidence available raises the possibility that organosulfur odorants may pose health risks at exposures much lower than presently understood, though additional dose-response studies are needed to disentangle specific toxicologic effects from nonspecific responses to noxious organosulfur odors. Numerous recommendations are provided including more transparent and prescriptive natural gas odorant use practices.
Topics: Animals; Humans; Odorants; Natural Gas; Asthma
PubMed: 37491689
DOI: 10.1007/s40572-023-00403-w -
Environment International Dec 2019Unwanted odour emissions are considered air pollutants that may cause detrimental impacts to the environment as well as an indicator of unhealthy air to the affected... (Review)
Review
Unwanted odour emissions are considered air pollutants that may cause detrimental impacts to the environment as well as an indicator of unhealthy air to the affected individuals resulting in annoyance and health related issues. These pollutants are challenging to handle due to their invisibility to the naked eye and can only be felt by the human olfactory stimuli. A strategy to address this issue is by introducing an intelligent processing system to odour monitoring instrument such as artificial neural network to achieve a robust result. In this paper, a review on the application of artificial neural network for the management of environmental odours is presented. The principal factors in developing an optimum artificial neural network were identified as elements, structure and learning algorithms. The management of environmental odour has been distinguished into four aspects such as measurement, characterization, control and treatment and continuous monitoring. For each aspect, the performance of the neural network is critically evaluated emphasizing the strengths and weaknesses. This work aims to address the scarcity of information by addressing the gaps from existing studies in terms of the selection of the most suitable configuration, the benefits and consequences. Adopting this technique could provide a new avenue in the management of environmental odours through the use of a powerful mathematical computing tool for a more efficient and reliable outcome.
Topics: Air Pollutants; Environmental Monitoring; Humans; Longitudinal Studies; Neural Networks, Computer; Odorants
PubMed: 31675561
DOI: 10.1016/j.envint.2019.105189 -
International Journal of Molecular... Jun 2019The olfactory sense is the dominant sensory perception for many animals. When Richard Axel and Linda B. Buck received the Nobel Prize in 2004 for discovering the G... (Review)
Review
The olfactory sense is the dominant sensory perception for many animals. When Richard Axel and Linda B. Buck received the Nobel Prize in 2004 for discovering the G protein-coupled receptors' role in olfactory cells, they highlighted the importance of olfaction to the scientific community. Several theories have tried to explain how cells are able to distinguish such a wide variety of odorant molecules in a complex context in which enantiomers can result in completely different perceptions and structurally different molecules. Moreover, sex, age, cultural origin, and individual differences contribute to odor perception variations that complicate the picture. In this article, recent advances in olfaction theory are presented, and future trends in human olfaction such as structure-based odor prediction and artificial sniffing are discussed at the frontiers of chemistry, physiology, neurobiology, and machine learning.
Topics: Animals; Electronic Nose; Humans; Machine Learning; Odorants; Olfactory Perception; Receptors, Odorant; Smell
PubMed: 31226833
DOI: 10.3390/ijms20123018 -
Current Biology : CB Jun 2023The olfactory system uses hundreds of odorant receptors (ORs), the largest group of the G-protein-coupled receptor (GPCR) superfamily, to detect a vast array of...
The olfactory system uses hundreds of odorant receptors (ORs), the largest group of the G-protein-coupled receptor (GPCR) superfamily, to detect a vast array of odorants. Each OR is activated by specific odorous ligands, and like other GPCRs, antagonism can block activation of ORs. Recent studies suggest that odorant antagonisms in mixtures influence olfactory neuron activities, but it is unclear how this affects perception of odor mixtures. In this study, we identified a set of human ORs activated by methanethiol and hydrogen sulfide, two potent volatile sulfur malodors, through large-scale heterologous expression. Screening odorants that block OR activation in heterologous cells identified a set of antagonists, including β-ionone. Sensory evaluation in humans revealed that β-ionone reduced the odor intensity and unpleasantness of methanethiol. Additionally, suppression was not observed when methanethiol and β-ionone were introduced simultaneously to different nostrils. Our study supports the hypothesis that odor sensation is altered through antagonistic interactions at the OR level.
Topics: Humans; Odorants; Receptors, Odorant; Smell; Perception; Olfactory Receptor Neurons; Olfactory Perception
PubMed: 37220745
DOI: 10.1016/j.cub.2023.04.072 -
The Yale Journal of Biology and Medicine Dec 2018Chemical communication involves the production, transmission, and perception of odors. Most adult insects rely on chemical signals and cues to locate food resources,... (Review)
Review
Chemical communication involves the production, transmission, and perception of odors. Most adult insects rely on chemical signals and cues to locate food resources, oviposition sites or reproductive partners and, consequently, numerous odors provide a vital source of information. Insects detect these odors with receptors mostly located on the antennae, and the diverse shapes and sizes of these antennae (and sensilla) are both astonishing and puzzling: what selective pressures are responsible for these different solutions to the same problem - to perceive signals and cues? This review describes the selection pressures derived from chemical communication that are responsible for shaping the diversity of insect antennal morphology. In particular, we highlight new technologies and techniques that offer exciting opportunities for addressing this surprisingly neglected and yet crucial component of chemical communication.
Topics: Animals; Arthropod Antennae; Biological Evolution; Odorants
PubMed: 30588211
DOI: No ID Found -
Environment International Jan 2020Odors have received increasing attention among atmospheric pollutants. Indeed, odor emissions are a common source of complaints, affecting the quality of life of humans... (Review)
Review
Odors have received increasing attention among atmospheric pollutants. Indeed, odor emissions are a common source of complaints, affecting the quality of life of humans and animals. The odor is a property of a mixture of different volatile chemical species (sulfur, nitrogen, and volatile organic compounds) capable of stimulating the olfaction sense sufficiently to trigger a sensation of odor. The impact of odors on the surrounding areas depends on different factors, such as the amount of odors emitted from the site, the distance from the site, weather conditions, topography, other than odors sensitivity and tolerance of the neighborhood. Due to the complexity of the odor issue, the aim of this review was to give an overview of: (i) techniques (sensorial and analytical) that can be used to determine a quantitative and qualitative characterization; (ii) air dispersion models applied for the evaluation of the spatial and temporal distribution of atmospheric pollutants in terms of concentration in air and/or deposition in the studied domain; (iii) major sources of odor nuisance (waste and livestock); (iv) mitigation actions against odor impact. Among sensorial techniques dynamic olfactometry, field inspection, and recording from residents were considered; whereas, for analytical methodologies: gas chromatography-mass spectrometry, identification of specific compounds, and electronic nose. Both kinds of techniques evaluate the odor concentration. Instead, to account for the effective impact of odors on the population, air dispersion models are used. They can provide estimates of odor levels in both current and future emission scenarios. Moreover, they can be useful to estimate the efficiency of mitigation strategies. Most of the odor control strategies involve measures oriented to prevent, control dispersion, minimize the nuisance or remove the odorants from emissions, such as adequate process design, buffer zones, odor covers, and treatment technologies.
Topics: Animals; Environmental Pollutants; Gas Chromatography-Mass Spectrometry; Humans; Odorants; Quality of Life
PubMed: 31704563
DOI: 10.1016/j.envint.2019.105261 -
Cellular and Molecular Life Sciences :... Feb 2018The sense of smell enables insects to recognize and discriminate a broad range of volatile chemicals in their environment originating from prey, host plants and... (Review)
Review
The sense of smell enables insects to recognize and discriminate a broad range of volatile chemicals in their environment originating from prey, host plants and conspecifics. These olfactory cues are received by olfactory sensory neurons (OSNs) that relay information about food sources, oviposition sites and mates to the brain and thus elicit distinct odor-evoked behaviors. Research over the last decades has greatly advanced our knowledge concerning the molecular basis underlying the reception of odorous compounds and the mechanisms of signal transduction in OSNs. The emerging picture clearly indicates that OSNs of insects recognize odorants and pheromones by means of ligand-binding membrane proteins encoded by large and diverse families of receptor genes. In contrast, the mechanisms of the chemo-electrical transduction process are not fully understood; the present status suggests a contribution of ionotropic as well as metabotropic mechanisms. In this review, we will summarize current knowledge on the peripheral mechanisms of odor sensing in insects focusing on olfactory receptors and their specific role in the recognition and transduction of odorant and pheromone signals by OSNs.
Topics: Animals; Insecta; Odorants; Olfactory Receptor Neurons; Pheromones; Receptors, Odorant; Signal Transduction; Smell
PubMed: 28828501
DOI: 10.1007/s00018-017-2627-5 -
Scientific Reports Nov 2022Deciphering the relationship between molecules, olfactory receptors (ORs) and corresponding odors remains a challenging task. It requires a comprehensive identification...
Deciphering the relationship between molecules, olfactory receptors (ORs) and corresponding odors remains a challenging task. It requires a comprehensive identification of ORs responding to a given odorant. With the recent advances in artificial intelligence and the growing research in decoding the human olfactory perception from chemical features of odorant molecules, the applications of advanced machine learning have been revived. In this study, Convolutional Neural Network (CNN) and Graphical Convolutional Network (GCN) models have been developed on odorant molecules-odors and odorant molecules-olfactory receptors using a large set of 5955 molecules, 160 odors and 106 olfactory receptors. The performance of such models is promising with a Precision/Recall Area Under Curve of 0.66 for the odorant-odor and 0.91 for the odorant-olfactory receptor GCN models respectively. Furthermore, based on the correspondence of odors and ORs associated for a set of 389 compounds, an odor-olfactory receptor pairwise score was computed for each odor-OR combination allowing to suggest a combinatorial relationship between olfactory receptors and odors. Overall, this analysis demonstrate that artificial intelligence may pave the way in the identification of the smell perception and the full repertoire of receptors for a given odorant molecule.
Topics: Humans; Artificial Intelligence; Odorants; Olfactory Perception; Olfactory Receptor Neurons; Receptors, Odorant; Smell
PubMed: 36335231
DOI: 10.1038/s41598-022-23176-y -
Chemical Senses Jan 2022Odor identification is a common assessment of olfaction, and it is affected in a large number of diseases. Identification abilities decline with age, but little is known...
Odor identification is a common assessment of olfaction, and it is affected in a large number of diseases. Identification abilities decline with age, but little is known about whether there are perceptual odor features that can be used to predict identification. Here, we analyzed data from a large, population-based sample of 2,479 adults, aged 60 years or above, from the Swedish National study on Aging and Care in Kungsholmen. Participants performed both free and cued odor identification tests. In a separate experiment, we assessed perceived pleasantness, familiarity, intensity, and edibility of all odors in the first sample, and examined how odor identification performance is associated with these variables. The analysis showed that high-intensity odors are easier to identify than low-intensity odors overall, but also that they are more susceptible to the negative repercussions of old age. This result indicates that sensory decline is a major aspect of age-dependent odor identification impairment, and suggests a framework where identification likelihood is proportional to the perceived intensity of the odor. Additional analyses further showed that high-performing individuals can discriminate target odors from distractors along the pleasantness and edibility dimensions and that unpleasant and inedible odors show smaller age-related differences in identification. Altogether, these results may guide further development and optimization of brief and efficient odor identification tests as well as influence the design of odorous products targeted toward older consumers.
Topics: Adult; Humans; Odorants; Smell; Recognition, Psychology; Aging; Emotions
PubMed: 36334272
DOI: 10.1093/chemse/bjac025