Did you mean: oligella urethral
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Revista Chilena de Infectologia :... Feb 2012
Topics: Alcaligenaceae
PubMed: 22552519
DOI: 10.4067/S0716-10182012000100016 -
Journal of Clinical Microbiology Aug 2019
PubMed: 31350376
DOI: 10.1128/JCM.01543-18 -
Journal of Clinical Microbiology Mar 1988We determined phenotypic characteristics, cellular fatty acid composition, and isoprenoid quinone content of representative strains of CDC groups EO-2, M-5, and M-6,...
Cultural and chemical characterization of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis.
We determined phenotypic characteristics, cellular fatty acid composition, and isoprenoid quinone content of representative strains of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis. All organisms contained ubiquinone with eight isoprene units as the major isoprenolog, but distinct differences were observed in fatty acid composition. Twenty-eight of the original collection of CDC group EO-2 strains were further identified as P. immobilis, EO-2, or EO-3 by distinctive cellular fatty acid profiles, cellular morphology, and pigment production. The cellular fatty acid compositions of M-5 and M-6 were similar but were clearly different from those of other organisms. The genus Acinetobacter was differentiated from other organisms in the study by small amounts of 2-hydroxydodecanoic acid (2-OH-12:0), and P. immobilis was differentiated by small amounts of decanoic acid (10:0) and a branched-chain 17-carbon acid (i-17:0). All Moraxella species were distinguished by small amounts of decanoic acid (10:0) and the absence of i-17:0. M. bovis, M. nonliquefaciens, and some strains of M. lacunata formed a single fatty acid group, while M. osloensis, M. phenylpyruvica, M. atlantae, and other strains of M. lacunata (M. lacunata II) had species-specific fatty acid profiles. O. urethralis differed from Moraxella species by the presence of large amounts (49%) of cis-vaccenic acid (18:1 omega 7c), small amounts (1%) of 3-hydroxyhexadecanoate (3-OH-16:0), and the absence of 10:0 and 3-hydroxydodecanoate (3-OH-12:0). The combined use of chemical data and a small number of conventional tests permitted rapid identification and differentiation of these organisms from each other and from related organisms.
Topics: Acinetobacter; Animals; Chromatography, Gas; Fatty Acids; Gram-Negative Bacteria; Humans; Minicomputers; Moraxella; Software; Transformation, Bacterial
PubMed: 3356788
DOI: 10.1128/jcm.26.3.484-492.1988 -
Cureus Feb 2023is a commensal bacteria genus of the human urinary tract that rarely precipitates clinical infections. We report the case of an asymptomatic 24-year-old male with a...
is a commensal bacteria genus of the human urinary tract that rarely precipitates clinical infections. We report the case of an asymptomatic 24-year-old male with a medical history of Tourette syndrome and the recent placement of deep brain stimulator leads, which were found to be co-infected with species during hardware implantation. This is the first reported case of a deep brain stimulator infection by , a potentially under-recognized and emerging opportunistic bacteria. We review the previously published cases of extra-genitourinary infections and detail the clinical management of this uncommon pathogen.
PubMed: 36949980
DOI: 10.7759/cureus.35133 -
Microbiology Resource Announcements Feb 2024are opportunistic pathogens typically associated with genitourinary infections. Here, we report the complete genome for an isolate recovered from ear discharge of a...
are opportunistic pathogens typically associated with genitourinary infections. Here, we report the complete genome for an isolate recovered from ear discharge of a child with chronic suppurative otitis media (strain MSHR-50412PR). The genome comprises 2.58 Mb, with 2,448 coding sequences and 46.26% average GC content.
PubMed: 38275301
DOI: 10.1128/mra.01071-23 -
Antimicrobial Agents and Chemotherapy May 2003Clinical Oligella urethralis isolate COH-1, which was uncommonly resistant to penicillins and narrow-spectrum cephalosporins, was recovered from a 55-year-old patient...
Clinical Oligella urethralis isolate COH-1, which was uncommonly resistant to penicillins and narrow-spectrum cephalosporins, was recovered from a 55-year-old patient with a urinary tract infection. Shotgun cloning into Escherichia coli and expression experiments gave recombinant clones expressing either an AmpC beta-lactamase-type phenotype of resistance or a carbenicillin-hydrolyzing beta-lactamase-type phenotype of resistance. The AmpC beta-lactamase identified (ABA-1), which had a pI value of 8.2, had 98% amino acid identity with a chromosomally encoded cephalosporinase of Acinetobacter baumannii. A 820-bp insertion sequence element, ISOur1, belonging to the IS6 family of insertion sequence elements, was identified immediately upstream of bla(ABA-1), providing a -35 promoter sequence and likely giving rise to a hybrid promoter region. The carbenicillin-hydrolyzing beta-lactamase identified (CARB-8), which had a pI value of 6.4, differed from CARB-5 by two amino acid substitutions. Hybridization of CeuI fragment I-restricted DNA fragments of O. urethralis COH-1 with bla(ABA-1)-, bla(CARB-8)-, and 16S rRNA-specific probes indicated the chromosomal integration of the beta-lactamase genes. PCR and hybridization experiments failed to detect bla(CARB-8)- and bla(ABA-1)-like genes in three O. urethralis reference strains, indicating that the beta-lactamase genes identified were the source of acquired resistance in O. urethralis COH-1. This is one of the few examples of the interspecies transfer and the chromosomal integration of a gene encoding a naturally occurring beta-lactamase.
Topics: Acinetobacter baumannii; Amino Acid Sequence; Anti-Bacterial Agents; Bacterial Proteins; Chromosomes, Bacterial; Cloning, Molecular; Conjugation, Genetic; Drug Resistance, Bacterial; Gram-Negative Aerobic Bacteria; Humans; Microbial Sensitivity Tests; Middle Aged; Molecular Sequence Data; beta-Lactamases; beta-Lactams
PubMed: 12709319
DOI: 10.1128/AAC.47.5.1536-1542.2003 -
Journal of Dairy Science Dec 1999Bulk tank milk from 131 dairy herds in eastern South Dakota and western Minnesota were examined for coliforms and noncoliform bacteria. Coliforms were detected in 62.3%...
Bulk tank milk from 131 dairy herds in eastern South Dakota and western Minnesota were examined for coliforms and noncoliform bacteria. Coliforms were detected in 62.3% of bulk tank milk samples. Counts ranged from 0 to 4.7 log10 cfu/ml. The mean count was 3.4 log10 cfu/ml. Gram-negative noncoliform bacteria were observed in 76.3% of bulk tank milk. Counts ranged from 0 to 6.2 log10 cfu/ml. The mean count was 4.8 log10 cfu/ml. A total of 234 isolates from bulk tank milk were examined to species level; 205 isolates belonged to 28 species. Coliforms and gram-negative noncoliform bacteria accounted for 32.9 and 67.1% of the total isolates, respectively. Organisms such as Agrobacterium radiobacter, Bordetella spp., Comamonas testosteroni, Listonella damsela, Ochrobactrum anthropi, and Oligella urethralis were isolated from bulk tank milk in this study. These organisms have not been reported previously in bulk tank milk. A total of 116 isolates of Pseudomonas spp. were isolated from raw milk; 98 isolates belonged to nine Pseudomonas spp., and the remaining 18 isolates could not be identified to their species level. Pseudomonas was the most predominant genus. Pseudomonas fluorescens was the most predominant species isolated from bulk tank milk and accounted for 29.9% of all isolates examined. The results of the study suggest that counts of coliforms and noncoliform bacteria in bulk tank milk vary considerably. The isolates represent a wide variety of Gram-negative bacterial species. Examination of bulk tank milk for coliforms and noncoliform bacteria could provide an indication of current and potential problems associated with bacterial counts and milk quality.
Topics: Animals; Colony Count, Microbial; Enterobacteriaceae; Gram-Negative Bacteria; Milk; Pseudomonas; Quality Control
PubMed: 10629809
DOI: 10.3168/jds.S0022-0302(99)75518-9 -
Journal of Clinical Microbiology Aug 2019
Topics: Alcaligenaceae; Fatal Outcome; Gram-Negative Bacterial Infections; Humans; Lung Abscess; Lung Neoplasms; Male; Middle Aged; Thorax; Tomography, X-Ray Computed
PubMed: 31350375
DOI: 10.1128/JCM.01542-18 -
The Journal of Veterinary Medical... Dec 1999In order to establish a rapid diagnostic method for contagious equine metritis (CEM), we developed and evaluated a polymerase chain reaction (PCR) test. Species-specific...
In order to establish a rapid diagnostic method for contagious equine metritis (CEM), we developed and evaluated a polymerase chain reaction (PCR) test. Species-specific PCR primer sets were derived from the DNA sequence of a cloned DNA fragment of Taylorella equigenitalis that did not hybridize with the genome of a taxomonically related species, Oligella urethralis. Single step PCR with primer set P1-N2 and two-step semi-nested PCR with primer sets P1-N2 and P2-N2 detected as low as 100 and 10 CFU of the bacteria, respectively. Single-step PCR detected T. equigenitalis from genital swabs of experimentally infected mares with sensitivity comparable to that of bacterial isolation. Furthermore, two-step PCR was more sensitive than the culture method. Upon examination of field samples, 12 out of 3,123 samples were positive by single-step PCR while only 2 were positive by bacterial culture. The 12 PCR-positive samples originated from 5 mares, of which 3 animals were considered to be carriers based on previous bacteriologic and serologic diagnoses for CEM. The PCR test described in this study would provide a specific and highly sensitive tool for the rapid diagnosis of CEM.
Topics: Animals; Base Sequence; Cervix Uteri; Clitoris; DNA Primers; DNA, Bacterial; Electrophoresis, Agar Gel; Endometritis; Female; Gene Library; Gram-Negative Bacterial Infections; Horse Diseases; Horses; Male; Molecular Sequence Data; Nucleic Acid Hybridization; Penis; Polymerase Chain Reaction; Sensitivity and Specificity; Sequence Analysis, DNA; Taylorella equigenitalis
PubMed: 10651048
DOI: 10.1292/jvms.61.1287 -
Atencion Primaria Nov 2001
Topics: Aged; Anti-Bacterial Agents; Female; Follow-Up Studies; Fosfomycin; Gram-Negative Bacterial Infections; Humans; Moraxella; Time Factors; Urinary Tract Infections; Urine
PubMed: 11747779
DOI: 10.1016/s0212-6567(01)70466-0