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FEBS Letters Sep 1969
PubMed: 11947242
DOI: 10.1016/0014-5793(69)80296-6 -
Nucleic Acids Research Dec 1985The preferred binding sites for mithramycin on four different DNA fragments have been investigated by DNAase I footprinting. Sites containing at least two contiguous GC...
The preferred binding sites for mithramycin on four different DNA fragments have been investigated by DNAase I footprinting. Sites containing at least two contiguous GC base pairs are protected by the antibiotic, the preferred binding site consisting of the dinucleotide step GpG (or CpC). Related antibiotics chromomycin and olivomycin produce similar, but not identical footprinting patterns suggesting that they can recognize other sequences as well. All three antibiotics induce enhanced rates of enzyme cleavage at regions flanking some of their binding sites. These effects are generally observed in runs of A and T and are attributed to DNA structural variations induced in the vicinity of the ligand binding site. The reaction of dimethylsulphate with N7 of guanine was modified by the presence of mithramycin so that we cannot exclude the possibility that these antibiotics bind to DNA via the major groove.
Topics: Base Sequence; Binding Sites; Chromomycins; DNA; Deoxyribonuclease I; Endodeoxyribonucleases; Nucleic Acid Conformation; Olivomycins; Plicamycin; Sulfuric Acid Esters
PubMed: 2934687
DOI: 10.1093/nar/13.24.8695 -
Cytometry May 1994Flow cytometry with the AT-specific fluorochrome Hoechst 33258 (HO) and the GC-specific fluorochrome olivomycin (OM) was used for measurement of base pair specific DNA...
Flow cytometry with the AT-specific fluorochrome Hoechst 33258 (HO) and the GC-specific fluorochrome olivomycin (OM) was used for measurement of base pair specific DNA content in 20 species of vertebrates. The results were found to be in good correlation with the biochemical literature on base pair frequencies (r = 0.972, P < 1 x 10(-8). This correlation allows one to determine the percent of GC/AT-pairs and genome size from flow cytometric data. The genome sizes obtained were compared with the literature data on flow cytometric genome size values determined with the use of propidium iodide (PI) that is usually believed to be non-base pair specific. The results were found to be in general agreement; however, the previously reported slight GC-preference of PI is confirmed. The optimal conditions for flow cytometry of AT/GC ratio and genome size with the use of OM and HO are discussed. The approach can be useful for research in ecology, fisheries science, species conservation, and other environmental studies as a tool for rapid survey of a vast array of specimens.
Topics: Animals; Base Composition; Bisbenzimidazole; DNA; Flow Cytometry; Genome; Humans; Linear Models; Olivomycins
PubMed: 7518377
DOI: 10.1002/cyto.990160106 -
Cytometry Feb 1998Genome size and GC-percent were determined by means of a special method of DNA flow cytometry in 154 vertebrate species. For the total dataset, a highly significant...
Genome size and GC-percent were determined by means of a special method of DNA flow cytometry in 154 vertebrate species. For the total dataset, a highly significant positive correlation was found between both parameters. The overall distribution of points is not linear but triangular: a wide range of GC-percent values is observed at the lower end of genome size range, whereas with an increase in genome size the lower limit for GC-percent is elevated, gradually approaching the upper limit (about 46%). In teleost fishes, which occupy the lower part of genome size range, the negative relationship between both parameters was observed. Two positive linear relationships were found between mean genome size and GC-percent of the main vertebrate groups (one includes fishes, amphibians, and mammals, the other consists of reptiles and birds, which show the higher GC-percent for their genome sizes). Distribution of variance between taxonomic levels indicates that GC-percent is more evolutionarily conservative than genome size in anamniotes. Anuran amphibians show the greatest part of genome size variability at the lower taxonomic levels as compared to other vertebrates (with no additional variance already above the genus level). The data obtained with different methods are compared. It is shown that the proposed method can provide useful data for studies on genome evolution and biodiversity.
Topics: Animals; Base Composition; DNA; Erythrocytes; Flow Cytometry; Genome; Molecular Weight; Olivomycins; Spleen; Vertebrates
PubMed: 9482279
DOI: 10.1002/(sici)1097-0320(19980201)31:2<100::aid-cyto5>3.0.co;2-q -
PloS One 2018[This corrects the article DOI: 10.1371/journal.pone.0191923.].
[This corrects the article DOI: 10.1371/journal.pone.0191923.].
PubMed: 29474505
DOI: 10.1371/journal.pone.0193630 -
Hereditas 1993Cells of 82 species of Tetrapoda were stained with DNA base pair specific fluorochromes (Hoechst 33258 and olivomycin) and studied by means of flow cytometry. The genome...
Cells of 82 species of Tetrapoda were stained with DNA base pair specific fluorochromes (Hoechst 33258 and olivomycin) and studied by means of flow cytometry. The genome size range was about 50-fold. The class Amphibia, which had the widest range of genome size variation (about 20-fold), exhibited linear allometry in their base pair specific DNA contents (bps-C-values), i.e., the more DNA they had, the lower the quotient of AT-pairs (C(AT) = 0.13 + 0.87 x C(GC), r = +0.998). Data for Mammalia, pooled with amphibians, fell on the same allometric line at the lower extreme end of genome size range, supporting the correlation. Reptilia-Aves (or Reptilia alone) pooled with Amphibia did not conform with this relationship. Reptilia-Aves form their own line (zone); pooled with Mammalia, this group showed no regularities in the relationship of their bps-DNA contents. Besides revealing the allometry of bps-C-values, these data indicate an integral genomic feature, localization within the same regression line, which Mammalia share with Amphibia but not with recent Reptilia (and Aves). These data also suggest that the relationship between DNA base frequencies and genome size is nonlinear (reciprocal); to obtain a linear relationship, the bps-C-values should be used. It is also concluded that caution is needed when DNA-content is measured for comparative purposes using a fluorescent dye which is known to be base-pair specific. DNA content values obtained with fluorochromes with different specificity may differ as much as by a factor of 1.8, the average discrepancy level is about 14%.
Topics: Animals; Base Composition; DNA; Flow Cytometry; Fluorescent Dyes; Humans
PubMed: 8335472
DOI: 10.1111/j.1601-5223.1993.00155.x -
The Journal of Antibiotics Aug 1977Antitumor antibiotics of the olivomycin and chromomycin class were transformed when incubated with a culture of Whetzelinia sclerotiorum. The products, when purified by...
Antitumor antibiotics of the olivomycin and chromomycin class were transformed when incubated with a culture of Whetzelinia sclerotiorum. The products, when purified by counter-current distribution and column chromatography, were shown, by their physical properties, to be the deacylated analogues.
Topics: Bacteria; Biotransformation; Chromomycins; Fungi; Mitosporic Fungi; Olivomycins
PubMed: 908662
DOI: 10.7164/antibiotics.30.635 -
Nucleic Acids Research 1979Several antibiotics, netropsin, distamycin A, actinomycin D, Hoechst 33258 and olivomycin, which demonstrate base specificity in their DNA binding properties have been...
Several antibiotics, netropsin, distamycin A, actinomycin D, Hoechst 33258 and olivomycin, which demonstrate base specificity in their DNA binding properties have been found to alter the electrophoretic mobility of DNA restriction fragments in native polyacrylamide gels. The antibiotics mostly reduced the migration of larger DNA fragments, but netropsin and Hoechst 33258 were observed to increase the migration rate of several DNA fragments of intermediate size. DNA fragments of similar molecular weight which comigrate as a single gel band can at times be separated as the result of differential mobility shifts promoted by antibiotic DNA complex formations.
Topics: Anti-Bacterial Agents; Chemical Phenomena; Chemistry; DNA Restriction Enzymes; DNA, Viral; Dactinomycin; Distamycins; Electrophoresis, Polyacrylamide Gel; Ethidium; Molecular Weight; Netropsin; Olivomycins
PubMed: 450716
DOI: 10.1093/nar/6.5.1869 -
FEBS Letters Mar 1989The nonintercalative groove binding of a simplified model of olivomycin, to sequences d(CGCGCGC)2, d(TATATAT)2, and d(CICICIC)2 is investigated. A significant preference... (Comparative Study)
Comparative Study
The nonintercalative groove binding of a simplified model of olivomycin, to sequences d(CGCGCGC)2, d(TATATAT)2, and d(CICICIC)2 is investigated. A significant preference is displayed for the minor groove of the d(CG) sequence. This is due predominantly to the formation of H-bonds between the hydroxyl groups on the aglycone of the drug and the 2-amino group of the central guanine of the oligonucleotide.
Topics: Base Sequence; Binding Sites; DNA; Hydrogen Bonding; Molecular Conformation; Olivomycins; Thermodynamics
PubMed: 2924917
DOI: 10.1016/0014-5793(89)80209-1