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The Journal of Membrane Biology Nov 2005Osmotically driven water flow, u (cm/s), between two solutions of identical osmolarity, c(o) (300 mM: in mammals), has a theoretical isotonic maximum given by u =...
Osmotically driven water flow, u (cm/s), between two solutions of identical osmolarity, c(o) (300 mM: in mammals), has a theoretical isotonic maximum given by u = j/c(o), where j (moles/cm(2)/s) is the rate of salt transport. In many experimental studies, transport was found to be indistinguishable from isotonic. The purpose of this work is to investigate the conditions for u to approach isotonic. A necessary condition is that the membrane salt/water permeability ratio, epsilon, must be small: typical physiological values are epsilon = 10(-3) to 10(-5), so epsilon is generally small but this is not sufficient to guarantee near-isotonic transport. If we consider the simplest model of two series membranes, which secrete a tear or drop of sweat (i.e., there are no externally-imposed boundary conditions on the secretion), diffusion is negligible and the predicted osmolarities are: basal = c(o), intracellular approximately (1 + epsilon)c(o), secretion approximately (1 + 2epsilon)c(o), and u approximately (1 - 2epsilon)j/c(o). Note that this model is also appropriate when the transported solution is experimentally collected. Thus, in the absence of external boundary conditions, transport is experimentally indistinguishable from isotonic. However, if external boundary conditions set salt concentrations to c(o) on both sides of the epithelium, then fluid transport depends on distributed osmotic gradients in lateral spaces. If lateral spaces are too short and wide, diffusion dominates convection, reduces osmotic gradients and fluid flow is significantly less than isotonic. Moreover, because apical and basolateral membrane water fluxes are linked by the intracellular osmolarity, water flow is maximum when the total water permeability of basolateral membranes equals that of apical membranes. In the context of the renal proximal tubule, data suggest it is transporting at near optimal conditions. Nevertheless, typical physiological values suggest the newly filtered fluid is reabsorbed at a rate u approximately 0.86 j/c(o), so a hypertonic solution is being reabsorbed. The osmolarity of the filtrate c(F) (M) will therefore diminish with distance from the site of filtration (the glomerulus) until the solution being transported is isotonic with the filtrate, u = j/c(F).With this steady-state condition, the distributed model becomes approximately equivalent to two membranes in series. The osmolarities are now: c(F) approximately (1 - 2epsilon)j/c(o), intracellular approximately (1 - epsilon)c(o), lateral spaces approximately c(o), and u approximately (1 + 2epsilon)j/c(o). The change in c(F) is predicted to occur with a length constant of about 0.3 cm. Thus, membrane transport tends to adjust transmembrane osmotic gradients toward epsilonc(o), which induces water flow that is isotonic to within order epsilon. These findings provide a plausible hypothesis on how the proximal tubule or other epithelia appear to transport an isotonic solution.
Topics: Animals; Biological Transport; Cell Membrane; Computer Simulation; Humans; Isotonic Solutions; Kidney Tubules, Proximal; Models, Biological; Osmosis
PubMed: 16596445
DOI: 10.1007/s00232-005-0817-9 -
Food Microbiology Feb 2023Ultrafiltration (UF) and reverse osmosis (RO) are commonly used for the clarification and concentration of fruit juices. However, one of the main limitations of...
Ultrafiltration (UF) and reverse osmosis (RO) are commonly used for the clarification and concentration of fruit juices. However, one of the main limitations of filtration membranes is biofouling, which reduces membrane efficiency and can contaminate the filtered product and lead to spoilage. In this study, the microbial fouling layers of UF and RO membranes from a Canadian cranberry juice processing plant were characterized. Unlike the microbiota found in cranberry juice, which is dominated by Bacillus sp. and other bacteria, both UF and RO membranes were mainly colonized by several strains of the yeast Candida krusei. A variation in bacterial and yeasts count was observed between tubular UF and spiral-wound RO membranes, and the analysis of the spatial distribution highlighted the homogeneity of the contamination across each membrane. Surprisingly, RO membranes had a higher level of contamination when compared to UF membranes. Furthermore, six strains of C. krusei were further characterized through multilocus sequence typing analysis, five of which exhibited unique allelic profiles and two of which were found to contain a new TRP1 allele.
Topics: Ultrafiltration; Vaccinium macrocarpon; Osmosis; Membranes, Artificial; Canada; Filtration; Bacteria
PubMed: 36309445
DOI: 10.1016/j.fm.2022.104146 -
Molecules (Basel, Switzerland) Aug 2023Phytochromes are bistable red/far-red light-responsive photoreceptor proteins found in plants, fungi, and bacteria. Light-activation of the prototypical phytochrome Cph1...
Phytochromes are bistable red/far-red light-responsive photoreceptor proteins found in plants, fungi, and bacteria. Light-activation of the prototypical phytochrome Cph1 from the cyanobacterium sp. PCC 6803 allows photoisomerization of the bilin chromophore in the photosensory module and a subsequent series of intermediate states leading from the red absorbing Pr to the far-red-absorbing Pfr state. We show here via osmotic and hydrostatic pressure-based measurements that hydration of the photoreceptor modulates the photoconversion kinetics in a controlled manner. While small osmolytes like sucrose accelerate Pfr formation, large polymer osmolytes like PEG 4000 delay the formation of Pfr. Thus, we hypothesize that an influx of mobile water into the photosensory domain is necessary for proceeding to the Pfr state. We suggest that protein hydration changes are a molecular event that occurs during photoconversion to Pfr, in addition to light activation, ultrafast electric field changes, photoisomerization, proton release and uptake, and the major conformational change leading to signal transmission, or simultaneously with one of these events. Moreover, we discuss this finding in light of the use of Cph1-PGP as a hydration sensor, e.g., for the characterization of novel hydrogel biomaterials.
Topics: Osmosis; Biocompatible Materials; Biological Transport; Electricity; Phytochrome
PubMed: 37630372
DOI: 10.3390/molecules28166121 -
The AAPS Journal Sep 2010Osmotically controlled implants yield precise zero-order drug delivery kinetics and are utilized in a number of applications. The implants deliver drugs for extended... (Review)
Review
Osmotically controlled implants yield precise zero-order drug delivery kinetics and are utilized in a number of applications. The implants deliver drugs for extended periods (weeks to years) and exhibit good in vivo/in vitro correlation. This paper reviews critical variables associated with these implants, with a focus on release rate testing. The extended-duration kinetics can be problematic when attempting to test for >70% cumulative delivery. An innovative scheme based on the scientific principles of operation of the system is described to ensure > 70% delivery at the target rate and duration for the DUROS Viadur (leuprolide acetate) implant.
Topics: Drug Implants; Osmosis; Pharmacokinetics
PubMed: 20490735
DOI: 10.1208/s12248-010-9199-8 -
Analytical Chemistry Dec 2021Nanopipettes are finding increasing use as nano "test tubes", with reactions triggered through application of an electrochemical potential between electrodes in the...
Nanopipettes are finding increasing use as nano "test tubes", with reactions triggered through application of an electrochemical potential between electrodes in the nanopipette and a bathing solution (bath). Key to this application is an understanding of how the applied potential induces mixing of the reagents from the nanopipette and the bath. Here, we demonstrate a laser scanning confocal microscope (LSCM) approach to tracking the ingress of dye into a nanopipette (20-50 nm diameter end opening). We examine the case of dianionic fluorescein under alkaline conditions (pH 11) and large applied tip potentials (±10 V), with respect to the bath, and surprisingly find that dye ingress from the bath into the nanopipette is not observed under either sign of potential. Finite element method (FEM) simulations indicate this is due to the dominance of electro-osmosis in mass transport, with electro-osmotic flow in the conventional direction at +10 V and electro-osmosis of the second kind acting in the same direction at -10 V, caused by the formation of significant space charge in the center of the orifice. The results highlight the significant deviation in mass transport behavior that emerges at the nanoscale and the utility of the combined LSCM and FEM approach in deepening understanding, which in turn should promote new applications of nanopipettes.
Topics: Microscopy, Confocal; Osmosis
PubMed: 34846865
DOI: 10.1021/acs.analchem.1c02371 -
Acta Biochimica Et Biophysica Sinica Jan 2022The pericellular matrix stiffness is strongly associated with its biochemical and structural changes during the aging and osteoarthritis progress of articular cartilage....
The pericellular matrix stiffness is strongly associated with its biochemical and structural changes during the aging and osteoarthritis progress of articular cartilage. However, how substrate stiffness modulates the chondrocyte regulatory volume decrease (RVD) and calcium signaling in chondrocytes remains unknown. This study aims to investigate the effects of substrate stiffness on the chondrocyte RVD and calcium signaling by recapitulating the physiologically relevant substrate stiffness. Our results showed that substrate stiffness induces completely different dynamical deformations between the cell swelling and recovering progresses. Chondrocytes swell faster on the soft substrate but recovers slower than the stiff substrate during the RVD response induced by the hypo-osmotic challenge. We found that stiff substrate enhances the cytosolic Ca oscillation of chondrocytes in the iso-osmotic medium. Furthermore, chondrocytes exhibit a distinctive cytosolic Ca oscillation during the RVD response. Soft substrate significantly improves the Ca oscillation in the cell swelling process whereas stiff substrate enhances the cytosolic Ca oscillation in the cell recovering process. Our work also suggests that the TRPV4 channel is involved in the chondrocyte sensing substrate stiffness by mediating Ca signaling in a stiffness-dependent manner. This helps to understand a previously unidentified relationship between substrate stiffness and RVD response under the hypo-osmotic challenge. A better understanding of substrate stiffness regulating chondrocyte volume and calcium signaling will aid the development of novel cell-instructive biomaterial to restore cellular functions.
Topics: Calcium; Calcium Signaling; Cartilage, Articular; Chondrocytes; Humans; Osmosis; Osteoarthritis
PubMed: 35130619
DOI: 10.3724/abbs.2021008 -
The Science of the Total Environment Nov 2022Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment....
Forward osmosis (FO), a membrane separation process driven by a natural concentration gradient, is served as a potential strategy in the aspect of wastewater treatment. In this work, a worthy attempt at aquaculture wastewater treatment using a self-made FO system was conducted, confirming it was a promising approach to treating aquaculture wastewater. Optimization of operational parameters of the FO system, including draw solution (DS) concentration, cross-flow velocity, and DS temperature, was systematically investigated to enhance the running efficiency. Different selected parameters highly influenced the water flux during the single-factor experiments, and the findings indicated that the optimal conditions were DS of 1.5 M, cross-flow velocity of 15 cm/s, and temperature of 32 °C with consideration of FO performance and economical cost. An excellent linear relationship between chemical oxygen demand (COD) changing multiples and operational parameters was obtained from experimental results, offering a great interception performance of organic contamination. On the basis of optimal operating conditions, membrane fouling experiments with different running time were conducted, and the microscopic morphology and element composition of the fouled membrane were also analyzed. The results demonstrated that a layer of cake was coated on the surface of the membrane, and the main elements in the fouling cake included C, O, Na, and S, which were highly determined by the component of the feed solution (FS) and working time. Afterward, the 60-h FO fouled membrane was cleaned under the method which combined hydraulic power and chemical agents, and the water flux recovered to 12.79 Lm h, proving a good performance for the recovery of water flux. This investigation showed that employing sucrose as DS was effective for reducing wastewater volume, and it provided an alternative choice and a sustainable way for the separation of organic pollutants from water resources.
Topics: Aquaculture; Environmental Pollutants; Membranes, Artificial; Osmosis; Sucrose; Wastewater; Water; Water Purification
PubMed: 35882325
DOI: 10.1016/j.scitotenv.2022.157573 -
Molecular Pharmaceutics Jun 2019Concentrated solutions of monoclonal antibodies have attracted considerable attention due to their importance in pharmaceutical formulations; yet, their tendency to...
Concentrated solutions of monoclonal antibodies have attracted considerable attention due to their importance in pharmaceutical formulations; yet, their tendency to aggregate and the resulting high viscosity pose considerable problems. Here we tackle this problem by a soft condensed matter physics approach, which combines a variety of experimental measurements with a patchy colloid model, amenable of analytical solution. We thus report results of structural antibodies and dynamic properties obtained through scattering methods and microrheological experiments. We model the data using a colloid-inspired approach, explicitly taking into account both the anisotropic shape of the molecule and its charge distribution. Our simple patchy model is able to disentangle self-assembly and intermolecular interactions and to quantitatively describe the concentration-dependence of the osmotic compressibility, collective diffusion coefficient, and zero shear viscosity. Our results offer new insights on the key problem of antibody formulations, providing a theoretical and experimental framework for a quantitative assessment of the effects of additional excipients or chemical modifications and a prediction of the resulting viscosity.
Topics: Antibodies; Colloids; Osmosis; Viscosity
PubMed: 31059276
DOI: 10.1021/acs.molpharmaceut.9b00019 -
Chemosphere Jan 2024The recent advancements in fabricating forward osmosis (FO) membranes have shown promising results in desalination and water treatment. Different methods have been... (Review)
Review
The recent advancements in fabricating forward osmosis (FO) membranes have shown promising results in desalination and water treatment. Different methods have been applied to improve FO performance, such as using mixed or new draw solutions, enhancing the recovery of draw solutions, membrane modification, and developing FO-hybrid systems. However, reliable methods to address the current issues, including reverse salt flux, fouling, and antibacterial activities, are still in progress. In recent decades, surface modification has been applied to different membrane processes, including FO membranes. Introducing nanochannels, bioparticles, new monomers, and hydrophilic-based materials to the surface layer of FO membranes has significantly impacted their performance and efficiency and resulted in better control over fouling and concentration polarization (CP) in these membranes. This review critically investigates the recent developments in FO membrane processes and fabrication techniques for FO surface-layer modification. In addition, this study focuses on the latest materials and structures used for the surface modification of FO membranes. Finally, the current challenges, gaps, and suggestions for future studies in this field have been discussed in detail.
Topics: Membranes, Artificial; Osmosis; Water Purification; Sodium Chloride; Hydrophobic and Hydrophilic Interactions
PubMed: 37890801
DOI: 10.1016/j.chemosphere.2023.140493 -
Journal of Environmental Management Mar 2022There is a need for water reuse technologies and applications to minimize the imminent water crisis, caused by the world population growth, the reduction of freshwater...
There is a need for water reuse technologies and applications to minimize the imminent water crisis, caused by the world population growth, the reduction of freshwater resources and the increasing water pollution. Fertilizer-drawn forward osmosis (FDFO) is a promising process capable of simultaneously extracting fresh water from low-quality sources as feed water (e.g., wastewater or greywater), while diluting fertilizer solutions for direct fertigation, avoiding the demand for freshwater for irrigation. Achieving an adequate level of dilution for direct fertigation is a key element to be evaluated for the implementation of FDFO. This study assessed the performance of the forward osmosis process to dilute fertilizer solutions to be applied directly in hydroponic systems. Experiments were carried out under conditions close to osmotic equilibrium to evaluate the process performance up to the maximum dilution point. Tests were carried out with individual and blended fertilizers (i.e., (NH)HPO or DAP, and KNO) used as draw solution (DS) and with deionized water or individual salts (NaCl, MgCl, NaSO, MgSO) in the feed solution (FS). Water fluxes and reverse salt fluxes indicated that both fertilizer DS composition and concentrations play a fundamental role in the process. Suitable nutrient concentrations to be directly applied without further dilution for N, P and K (119, 40, 264 mg.L respectively) were obtained with deionized water as FS and blended DAP (0.025 M) and KNO (0.15 M) as DS. However, important fertilizer losses from DS to FS were observed, being the highest for NO (33-70% losses from DS to FS). The presence of salts in FS decreased the water fluxes and the DS dilution due to the osmotic equilibrium caused by a greater loss of nutrients from DS to FS (up to 100%), compared with tests using just deionized water as FS. This study points out the potential limitations of the FDFO process, due to the high solute fluxes and low water fluxes in conditions close to osmotic equilibrium.
Topics: Fertilizers; Hydroponics; Membranes, Artificial; Osmosis; Water Purification
PubMed: 34954684
DOI: 10.1016/j.jenvman.2021.114339