-
BioMed Research International 2013Vaginal probiotics have an important role in preventing the colonization of the vagina by pathogens. This study aimed to investigate different formulations with...
Vaginal probiotics have an important role in preventing the colonization of the vagina by pathogens. This study aimed to investigate different formulations with Pediococcus pentosaceus SB83 (lyophilized powder and tablets with and without retarding polymer) in order to verify its stability and antilisterial activity after manufacture and during storage. The bacteriocinogenic activity of P. pentosaceus SB83 against Listeria monocytogenes was evaluated in simulated vaginal fluid. Suspension of Pediococcus pentosaceus SB83 reduced the pathogen only after 2 h and the lyophilized bacteria after 24 h of contact, and, in the tablets, P. pentosaceus SB83 lost the antimicrobial activity. The pH of simulated vaginal fluid decreased for all the tested conditions. As lyophilized powder demonstrated better results concerning antimicrobial activity, this formulation was selected to evaluate the antilisterial activity during the 12 months of storage. During storage at room temperature, lyophilized bacteria totally inhibited the pathogen only until one month of storage. At 4°C, P. pentosaceus SB83 showed antimicrobial activity during all the time of storage investigated. Therefore, the better formulation of P. pentosaceus SB83 is the lyophilized powder stored at 4°C, which may be administered intravaginally as a washing solution.
Topics: Anti-Infective Agents; Body Fluids; Chemistry, Pharmaceutical; Female; Freeze Drying; Humans; Hydrogen-Ion Concentration; Listeria monocytogenes; Microbial Sensitivity Tests; Microbial Viability; Pediococcus; Powders; Tablets; Vagina
PubMed: 23844367
DOI: 10.1155/2013/680767 -
Polish Journal of Microbiology Jun 2022is an important microorganism in wine-making-related engineering, and it improves wine quality and stability through malolactic fermentation. Although the genomes of...
is an important microorganism in wine-making-related engineering, and it improves wine quality and stability through malolactic fermentation. Although the genomes of more than 200 strains have been sequenced, only a few include completed genome maps. Here, the genome sequence of SD-2a, isolated from Shandong, China, has been determined. It is a fully assembled genome sequence of this strain. The complete genome is 1,989,703 bp with a G+C content of 37.8% without a plasmid. The genome includes almost all the essential genes involved in central metabolic pathways and the stress genes reported in other strains. Some natural competence-related genes, like EA, EC, FA, G operon, and FC, suggest that SD-2a may have natural transformation potential. A comparative genomics analysis revealed 730 gene clusters in SD-2a homologous to those in four other lactic acid bacteria species ( PSU-1, CRBO-11381, UNQLp11, and KCCM40703). A collinearity analysis showed poor collinearity between SD-2a and PSU-1, indicating great differences in their evolutionary histories. The results provide general knowledge of SD-2a and lay the foundation for specific gene function analyses.
Topics: Fermentation; Genomics; Oenococcus; Wine
PubMed: 35716166
DOI: 10.33073/pjm-2022-026 -
Nutrients May 2019Wild ginseng is known to contain additional physiologically and pharmacologically active substances than common ginseng. The utilization of this herb can be maximized by...
Wild ginseng is known to contain additional physiologically and pharmacologically active substances than common ginseng. The utilization of this herb can be maximized by altering its composition via tissue culture generating adventitious roots. We enriched the content of specific ginsenosides and investigated their role in ameliorating memory impairment. Cultured wild ginseng root was subjected to extraction, steaming, and fermentation using to enhance the levels of ginsenosides Rg5 /Rk1. The analysis of product, HLJG0701, confirmed target ginsenosides. We analyzed the inhibitory effect of ginsenoside Rg5/Rk1, HLJG0701 and the raw material on acetylcholinesterase. Further, we performed Morris water maze, Y-maze, and passive avoidance tasks with mice exhibiting memory deficit induced by scopolamine, and we analyzed the concentrations of acetylcholinesterase and acetylcholine in their brains. Studies showed that the levels of ginsenosides Rg5 /Rk1, not found in the raw material, were enhanced in HLJG0701. Ginsenosides and HLJG0701 significantly inhibited acetylcholinesterase unlike the raw material. In all behavioral tasks, HLJG0701 showed memory improvement. It reduced acetylcholinesterase, whereas, it preserved acetylcholine in brain. In conclusion, cultured wild ginseng root extract fermented by contains the distinctive ginsenosides Rg5/Rk1, which may ameliorate memory impairment via inhibition of acetylcholinesterase resulting in increased acetylcholine levels in the brain.
Topics: Acetylcholinesterase; Animals; Avoidance Learning; Behavior, Animal; Brain; Cholinesterase Inhibitors; Disease Models, Animal; Fermentation; GPI-Linked Proteins; Ginsenosides; Male; Maze Learning; Memory; Memory Disorders; Mice, Inbred C57BL; Panax; Pediococcus pentosaceus; Plant Extracts; Plant Roots; Scopolamine
PubMed: 31137483
DOI: 10.3390/nu11051120 -
Heliyon Dec 2021Fermented products, including and , provide several health benefits, particularly when probiotics are used in the fermentation process. Probiotic microorganisms exert...
Fermented products, including and , provide several health benefits, particularly when probiotics are used in the fermentation process. Probiotic microorganisms exert strain-specific health-promoting activities on humans and animals. The objective of this study was to investigate the probiotic potentials of Lactic-acid bacteria (LAB) isolates from indigenous fermented sorghum products ( and ). The LAB isolates were screened for potential probiotic properties by antagonistic activity against eight enteropathogenic clinical bacteria isolates ( sp. sp. sp. sp. sp. and ) as indicator organisms using the agar well diffusion technique. The organisms were also screened for acidity, bile tolerance, antibiotic susceptibility, production of lactic acid, diacetyl and hydrogen peroxide. β-galactosidase assay was also done. Genomic DNA was extracted from the two selected LAB isolates; the 16S rRNA were amplified and sequenced. The sequence data were subjected to Basic Local Alignment Search Tool (BLAST) and molecular phylogenetic analyses to identify the isolates. The isolates were identified as strains of and The sequence data for these two isolates were submitted to the Genbank with accession numbers KP883298 and KP883297 respectively. The strain (PB2) strain exhibited β-galactosidase activity as well as strain (OB6). The study revealed exceptional probiotic potentials of two LAB namely strain (OB6) and strain (PB2) isolated from fermented sorghum products, and respectively. Hence, the two LAB strains may be potentially used as probiotic to prevent some enteropathogen-induced gastrointestinal disorders; reduce the incidence of respiratory tract infections and for the management of lactose in intolerance.
PubMed: 34926862
DOI: 10.1016/j.heliyon.2021.e08536 -
Microbial Biotechnology May 2021Prokaryotic laccases are emergent biocatalysts. However, they have not been broadly found and characterized in bacterial organisms, especially in lactic acid bacteria....
Prokaryotic laccases are emergent biocatalysts. However, they have not been broadly found and characterized in bacterial organisms, especially in lactic acid bacteria. Recently, a prokaryotic laccase from the lactic acid bacterium Pediococcus acidilactici 5930, which can degrade biogenic amines, was discovered. Thus, our study aimed to shed light on laccases from lactic acid bacteria focusing on two Pediococcus laccases, P. acidilactici 5930 and Pediococcus pentosaceus 4816, which have provided valuable information on their biochemical activities on redox mediators and biogenic amines. Both laccases are able to oxidize canonical substrates as ABTS, ferrocyanide and 2,6-DMP, and non-conventional substrates as biogenic amines. With ABTS as a substrate, they prefer an acidic environment and show sigmoidal kinetic activity, and are rather thermostable. Moreover, this study has provided the first structural view of two lactic acid bacteria laccases, revealing new structural features not seen before in other well-studied laccases, but which seem characteristic for this group of bacteria. We believe that understanding the role of laccases in lactic acid bacteria will have an impact on their biotechnological applications and provide a framework for the development of engineered lactic acid bacteria with enhanced properties.
Topics: Bacteria; Laccase; Oxidation-Reduction; Pediococcus; Prokaryotic Cells
PubMed: 33635570
DOI: 10.1111/1751-7915.13751 -
New Microbes and New Infections Jul 2019Consumer demand for natural pathogen-control agents for substitution of synthetic food preservatives and traditional antibiotics is increasing. This study aimed to...
Consumer demand for natural pathogen-control agents for substitution of synthetic food preservatives and traditional antibiotics is increasing. This study aimed to reveal the distribution of lactic acid bacteria (LAB) in raw camel milk and to characterize their antimicrobial traits. The genetic identification by 16S rRNA sequencing of 58 LAB isolates showed the predominance of (24.2%), (22.4%) and (20.7%) genera in raw camel milk. These genera exhibited inhibitory activity against a broad spectrum of Gram-positive and Gram-negative bacteria including multidrug-resistant . Among these LAB, two isolates-identified as CM16 and CM22-were selected for their strong bacteriocinogenic anti-listerial activity estimated at 1600 and 800 AU/mL, respectively. The bacteriocins produced were partially purified by ammonium sulphate precipitation and gel filtration and then biochemically characterized. The proteinaceous nature of bacteriocins was confirmed by the susceptibility to enzymes. These bacteriocins showed significant technological characteristics such as heat-resistance, and stability over a wide range of pH (2.0-10.0). In conclusion, these results indicated that CM16 and CM22 could be useful as potential probiotics. Moreover, their partially purified bacteriocins may play an important role as food preservatives and feed additives. To our knowledge, this is the first report describing the distribution of LAB population in raw camel milk and the characterization of their bacteriocins from the Arabian Peninsula of western Asia.
PubMed: 31193267
DOI: 10.1016/j.nmni.2019.100560 -
Gut Microbes Jul 2020Targeting the gut-liver axis by modulating the gut-microbiome can be a promising therapeutic approach in nonalcoholic fatty liver disease (NAFLD). The aim of this study...
Targeting the gut-liver axis by modulating the gut-microbiome can be a promising therapeutic approach in nonalcoholic fatty liver disease (NAFLD). The aim of this study was to evaluate the effects of single species and a combination of and in NAFLD mice model. Six-week male C57BL/6J mice were divided into 9 groups (n = 10/group; normal, Western diet, and 7 Western diet-strains [10 CFU/g, 8 weeks]). The strains used were KID7, and three combinations (1: , 2: KID7, and 3: ). Liver/Body weight ratio, serum and stool analysis, liver pathology, and metagenomics by 16S rRNA-sequencing were examined. In the liver/body ratio, (5.1 ± 0.5), (5.2 ± 0.4), KID7 (5.5 ± 0.5), and combination1 and 2 (4.2 ± 0.6 and 4.8 ± 0.7) showed significant reductions compared with Western (6.2 ± 0.6)(p < 0.001). In terms of cholesterol and steatosis/inflammation/NAFLD activity, all groups except for were associated with an improvement ( < .05). The elevated level of tumor necrosis factor-α/interleukin-1β (pg/ml) in Western (65.8 ± 7.9/163.8 ± 12.2) was found to be significantly reduced in (24.2 ± 1.0/58.9 ± 15.3), (35.6 ± 2.1/62.9 ± 6.0), (43.4 ± 3.2/53.6 ± 7.5), and KID7 (22.9 ± 3.4/59.7 ± 12.2)(p < 0.01). Cytokines were improved in the combination groups. In metagenomics, each strains revealed a different composition and elevated / ratio in the western (47.1) was decreased in (14.5), (3.0), and KID7 (13.3). , and KID7 supplementation can improve NAFLD-progression by modulating gut-microbiome and inflammatory pathway.
Topics: Animals; Bacteroidetes; Cholesterol; Cytokines; Diet, Western; Disease Models, Animal; Disease Progression; Firmicutes; Gastrointestinal Microbiome; Inflammation; Lactobacillus; Liver; Male; Metagenomics; Mice; Mice, Inbred C57BL; Non-alcoholic Fatty Liver Disease; Pediococcus pentosaceus; Probiotics
PubMed: 31965894
DOI: 10.1080/19490976.2020.1712984 -
Foods (Basel, Switzerland) Dec 2022In the present study, kashk samples were collected from two regions of Iran, the Fars (Abadeh) and Razavi Khorasan (Kalat) provinces. Fifteen bacteria were isolated and...
In the present study, kashk samples were collected from two regions of Iran, the Fars (Abadeh) and Razavi Khorasan (Kalat) provinces. Fifteen bacteria were isolated and physiological and biochemical assays were performed. After identification to the genus level, eight isolates were identified as lactic acid bacteria (LAB) and subjected to molecular identification and probiotic properties assays. The results revealed that the isolates were KKP 3772 (KF1), C1 (KF2), H11 (KF3), VNK-1 (KK4), RSg (KK1), P190052 (KK2), CECT972T (KK3), and PM411 (KK5). Only the numbers of RSg (KK1) and PM411 (KK5) decreased to below 9 Log CFU/mL after acidic conditions (pH = 3) and showed weak antibacterial activity. CECT972T (KK3) and C1(KF2) were highly susceptible to bile salts, while VNK-1 (KK4) and H11 (KF3) showed the highest resistance. All of the isolates were resistant to tetracycline and sensitive to chloramphenicol and gentamicin. The antimicrobial activity of VNK-1 (KK4) and H11 (KF3) was higher than other isolates and consequently, their inhibition zones were larger. The adhesion capabilities of LAB isolates to intestinal epithelial cells were evaluated by examining the auto-aggregation factor and cell surface hydrophobicity. The highest and lowest cell surface hydrophobicity and auto-aggregation were obtained from VNK-1 (KK4) and CECT972T (KK3), respectively. In general, VNK-1 (KK4) and H11 (KF3) have shown better probiotic properties as compared to other isolates.
PubMed: 36496711
DOI: 10.3390/foods11233904 -
Journal of Bacteriology Oct 1962Dobrogosz, Walter J. (Pennsylvania State University, University Park) and Robert W. Stone. Oxidative metabolism in Pediococcus pentosaceus. I. Role of oxygen and...
Dobrogosz, Walter J. (Pennsylvania State University, University Park) and Robert W. Stone. Oxidative metabolism in Pediococcus pentosaceus. I. Role of oxygen and catalase. J. Bacteriol. 84:716-723. 1962.-Studies were conducted on the physiological behavior of several strains of Pediococcus pentosaceus isolated from alfalfa silages. Although these organisms are regarded as homofermentative lactic acid bacteria which metabolize carbohydrates via the classic reactions of glycolysis, this investigation showed that they were capable of developing other physiologically important reactions related to carbohydrate metabolism. Growth on glycerol, for example, was shown to depend on the development of aerobic reactions, and was directly related to the catalase content of the various strains tested. These organisms were shown to be devoid of a cytochrome system, thus implicating an active flavoprotein system in oxidative reactions. A study of the end products of aerobic glycerol metabolism suggested that glycerol was oxidized to the pyruvate level, with subsequent reactions involving pyruvate leading to the accumulation of lactate, acetate, acetoin, and CO(2) in a molar ratio of approximately 1:1:1:3.
Topics: Acetates; Carbohydrate Metabolism; Catalase; Cell Respiration; Energy Metabolism; Glycerol; Lactates; Lactic Acid; Oxidation-Reduction; Oxygen; Pediococcus; Pyruvates
PubMed: 14028239
DOI: 10.1128/jb.84.4.716-723.1962 -
Food Science of Animal Resources Sep 2023We studied the proteolysis and conducted a sensory evaluation of fermented sausages using strains derived from [-SMFM2021-GK1 (GK1); -SMFM2021-NK3 (NK3)], [SMFM2021-D1...
We studied the proteolysis and conducted a sensory evaluation of fermented sausages using strains derived from [-SMFM2021-GK1 (GK1); -SMFM2021-NK3 (NK3)], [SMFM2021-D1 (D1)], and spontaneous fermented sausage [SMFM2021-S6 (S6)]. Fermented sausages were classified as commercial starter culture (CST), mixed with GK1, D1, and S6 (GKDS), and mixed with NK3, D1, and S6 (NKDS). The protein content and pH of GKDS and NKDS were significantly higher than those of CST on days 3 and 31, respectively (p<0.05). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the NKDS had higher molecular weight proteins than the GKDS and CST. The myofibrillar protein solubility of the GKDS and NKDS was significantly higher than that of the CST on day 31 (p<0.05). The GKDS displayed significantly higher pepsin and trypsin digestion than the NKDS on day 31 (p<0.05). The hardness, chewiness, gumminess, and cohesiveness of the GKDS were not significantly different from those of the CST. The GKDS exhibited the highest values for flavor, tenderness, texture, and overall acceptability. According to this study, sausages fermented using lactic acid bacteria (GK1), yeast (D1), and mold (S6) derived from Korean fermented foods displayed high proteolysis and excellent sensory evaluation results.
PubMed: 37701739
DOI: 10.5851/kosfa.2023.e42