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The Plant Cell Sep 2023
Topics: RNA, Long Noncoding
PubMed: 37433057
DOI: 10.1093/plcell/koad194 -
Foods (Basel, Switzerland) Jul 2020The beer production chain includes some crucial steps regarding processing, delivery, service, and consumption that can benefit from the implementation of IoT (Internet... (Review)
Review
The beer production chain includes some crucial steps regarding processing, delivery, service, and consumption that can benefit from the implementation of IoT (Internet of Things) based technologies. Large breweries implemented the use of sensors and digitization before smaller ones among which are craft breweries. Internet of Beer (IoB) technologies are becoming accessible to mid and small sized brewing companies. Therefore, the objective of this work is to review mainly low-cost IoB smart technologies that can be implemented from the mash to the final product and its service, to improve the brewing production, control, delivery, and final quality increasing profitability. The reviewed applications were retrieved both from the scientific databases and from the web. The work is structured in three macro areas such as beer processing, product logistics and traceability, and service. The results show a future trend characterized by a very fast increase in the use of IoB (also open source) systems to drive efficiency, productivity, quality, and safety. This will be done by real-time monitoring and a data-driven decision support system (DSS). Crucial aspects needing further investigation are data ownership and data standardization. The access price of IoB devices and software is destined for a significant decrease while their diversification on the market will grow leading to a massive future implementation within all the production levels.
PubMed: 32709156
DOI: 10.3390/foods9070950 -
Diabetes Care Mar 2022We tested whether the concurrence of food intake and elevated concentrations of endogenous melatonin, as occurs with late eating, results in impaired glucose control, in... (Randomized Controlled Trial)
Randomized Controlled Trial
OBJECTIVE
We tested whether the concurrence of food intake and elevated concentrations of endogenous melatonin, as occurs with late eating, results in impaired glucose control, in particular in carriers of the type 2 diabetes-associated G allele in the melatonin receptor-1B gene (MTNR1B).
RESEARCH DESIGN AND METHODS
In a Spanish natural late-eating population, a randomized, crossover study was performed. Each participant (n = 845) underwent two evening 2-h 75-g oral glucose tolerance tests following an 8-h fast: an early condition scheduled 4 h prior to habitual bedtime ("early dinner timing") and a late condition scheduled 1 h prior to habitual bedtime ("late dinner timing"), simulating an early and a late dinner timing, respectively. Differences in postprandial glucose and insulin responses between early and late dinner timing were determined using incremental area under the curve (AUC) calculated by the trapezoidal method.
RESULTS
Melatonin serum levels were 3.5-fold higher in the late versus early condition, with late dinner timing resulting in 6.7% lower insulin AUC and 8.3% higher glucose AUC. The effect of late eating impairing glucose tolerance was stronger in the MTNR1B G-allele carriers than in noncarriers. Genotype differences in glucose tolerance were attributed to reductions in β-cell function (P for interaction, Pint glucose area under the curve = 0.009, Pint corrected insulin response = 0.022, and Pint disposition index = 0.018).
CONCLUSIONS
Concurrently high endogenous melatonin and carbohydrate intake, as typical for late eating, impairs glucose tolerance, especially in MTNR1B G-risk allele carriers, attributable to insulin secretion defects.
Topics: Blood Glucose; Cross-Over Studies; Diabetes Mellitus, Type 2; Eating; Genotype; Glucose; Humans; Insulin; Insulin Secretion; Meals; Melatonin; Receptor, Melatonin, MT2; Risk Factors; Spain; Time Factors
PubMed: 35015083
DOI: 10.2337/dc21-1314 -
Frontiers in Pharmacology 2022Resistance to cisplatin (DDP) is a major obstacle in the clinical treatment of advanced gastric cancer (GC). Long noncoding RNA (lncRNA) play a significant regulatory...
Resistance to cisplatin (DDP) is a major obstacle in the clinical treatment of advanced gastric cancer (GC). Long noncoding RNA (lncRNA) play a significant regulatory role in the development and drug resistance of GC. In this study, we reported that the lncRNA LINC-PINT was downregulated in DDP-resistant GC cells. Functional studies showed that LINC-PINT inhibited proliferation and migration of DDP-resistant GC cells , and overexpression of LINC-PINT could enhance the sensitivity of DDP-resistant GC cells to DDP. Further investigation revealed that LINC-PINT recruited enhancer of zeste homolog 2 (EZH2) to the promotor of ATG5 to inhibit its transcription, leading to the suppression of autophagy and DDP resensitization. Collectively, our results revealed how the LINC-PINT/EZH2/ATG5 axis regulates autophagy and DDP resistance in GC. These data suggest that LINC-PINT may be a potential therapeutic target in GC.
PubMed: 36091809
DOI: 10.3389/fphar.2022.968223 -
Cancer Epidemiology, Biomarkers &... Apr 2022Although survival has improved dramatically for most adolescents and young adults (AYA; 15-39 years old) with cancer, it remains poor for those presenting with...
BACKGROUND
Although survival has improved dramatically for most adolescents and young adults (AYA; 15-39 years old) with cancer, it remains poor for those presenting with metastatic disease. To better characterize this subset, we conducted a landscape survival comparison with older adults (40-79 years).
METHODS
Using Surveillance, Epidemiology, and End Results Program data from 2000 to 2016, we examined incident cases of poor-prognosis metastatic cancers (5-year survival < 50%) among AYAs (n = 11,518) and older adults (n = 345,681) and compared cause-specific survival by sociodemographic characteristics (race/ethnicity, sex, and socioeconomic status). Adjusted HRs (aHR) for death from metastatic disease [95% confidence intervals (95% CI)] were compared between AYAs and older adults (Pint).
RESULTS
AYAs had significantly better survival than older adults for every cancer site except kidney, where it was equivalent (range of aHRs = 0.91; 95% CI, 0.82-1.02 for kidney cancer to aHR = 0.33; 95% CI, 0.26-0.42 for rhabdomyosarcoma). Compared with their older adult counterparts, greater survival disparities existed for AYAs who were non-Hispanic Black with uterine cancer (aHR = 2.20; 95% CI, 1.25-3.86 versus aHR = 1.40; 95% CI, 1.28-1.54; Pint = 0.049) and kidney cancer (aHR = 1.51; 95% CI, 1.15-1.98 versus aHR = 1.10; 95% CI, 1.03-1.17; Pint = 0.04); non-Hispanic Asian/Pacific Islanders with ovarian cancer (aHR = 1.47; 95% CI, 1.12-1.93 versus aHR = 0.89; 95% CI, 0.84-0.95; Pint<0.001); and males with colorectal cancer (aHR = 1.21; 95% CI, 1.10-1.32 versus aHR = 1.08; 95% CI, 1.06-1.10; Pint = 0.045).
CONCLUSIONS
AYAs diagnosed with these metastatic cancers have better survival than older adults, but outcomes remain dismal.
IMPACT
Overcoming the impact of metastasis in these cancers is necessary for continuing progress in AYA oncology. Sociodemographic disparities affecting AYAs within kidney, uterine, ovarian, and colorectal cancer could indicate plausible effects of biology, environment, and/or access and should be explored.
Topics: Adolescent; Adult; Aged; Ethnicity; Humans; Kidney Neoplasms; Male; Prognosis; Research; Social Class; Young Adult
PubMed: 35086824
DOI: 10.1158/1055-9965.EPI-21-0913 -
Frontiers in Cell and Developmental... 2019Long non-coding RNAs (lncRNAs) have been identified as crucial regulators in many human cancers. Many lncRNAs show aberrant expression in cancer, and some of them play...
Long non-coding RNAs (lncRNAs) have been identified as crucial regulators in many human cancers. Many lncRNAs show aberrant expression in cancer, and some of them play critical roles in tumor proliferation, invasion, and metastasis. However, the regulatory functions of lncRNAs in melanoma progression remain to be elucidated. We utilized the Real-time PCR methodology to determine the expression of LINC-PINT in melanoma cell lines. To evaluate the effect of LINC-PINT on tumorigenesis of melanoma, we used Cell Counting Kit-8 (CCK8) and colony formation assay. Flow cytometry assay was used to detect the function of LINC-PINT on cell cycle status. PINT-interacting proteins were identified by chromatin isolation using RNA purification (ChIRP). Microarray assay and bioinformatics analysis were used to find the potential target genes of LINC-PINT and the status of LINC-PINT target gene candidate was verified using chromatin immunoprecipitation assay (ChIP). LINC-PINT plays a role in suppressing the tumorigenicity of melanoma, which was further determined by xenograft model assay. LINC-PINT was significantly downregulated in melanoma tissues and cell lines. The overexpression of LINC-PINT in tumor cells resulted in significant tumor growth reduction and migration inhibition in A375, Mum2B and CRMM1 cells. Results based on the xenograft model were further consistent with the findings that LINC-PINT impeded growth and metastasis of melanoma cells. Microarray assay and bioinformatics analysis indicated that CDK1, CCNA2, AURKA, and PCNA were potential targets of LINC-PINT. In conclusion, LINC-PINT inhibits the tumorigenicity of melanoma through recruiting EZH2 to the promoter of its target genes, leading to H3K27 trimethylation and epigenetic silencing of target genes. LINC-PINT may serve as a novel diagnostic and therapeutic target for melanoma.
PubMed: 31921860
DOI: 10.3389/fcell.2019.00350 -
Scientific Reports Jan 2024The association between sarcopenia and OA still presents many uncertainties. We aimed to assess whether sarcopenia is associated with occurrence of OA in US adults. We...
The association between sarcopenia and OA still presents many uncertainties. We aimed to assess whether sarcopenia is associated with occurrence of OA in US adults. We conducted a cross-sectional study consisting of 11,456 participants from National Health and Nutrition Examination Survey 1999-2006. Sarcopenia was defined by a low muscle mass. The skeletal muscle index (SMI) was calculated as the appendicular skeletal muscle mass divided by body mass indexes (BMI) or body weight. OA status was assessed by using self-reported questionnaire. We evaluated the association between sarcopenia and OA using multivariate regression models. In addition, subgroup and interaction analysis were performed. Sarcopenia was associated with OA when it was defined by the BMI-adjusted SMI (OR = 1.23 [95% CI, 1.01, 1.51]; P = 0.038) and defined by the weight-adjusted SMI (OR = 1.30 [95% CI, 1.10, 1.55]; P = 0.003). Subgroup and interaction analysis found that the strongest positive association mainly exists in smoker (OR = 1.54 [95% CI, 1.21, 1.95], Pint = 0.006), and this association is not significant in other groups. In conclusion, we found that sarcopenia was associated with occurrence of OA. Subgroup analysis revealed that the association between sarcopenia and OA was more pronounced in smoker. Further well-designed prospective cohort studies are needed to assess our results.
Topics: Adult; Humans; Sarcopenia; Cross-Sectional Studies; Nutrition Surveys; Prospective Studies; Muscle, Skeletal; Osteoarthritis
PubMed: 38167445
DOI: 10.1038/s41598-023-50528-z -
Oncology Letters Sep 2019The development of melanoma may involve long non-coding RNAs (lncRNAs); however, the functions of the majority of lncRNAs in melanoma are unknown. The present study...
The development of melanoma may involve long non-coding RNAs (lncRNAs); however, the functions of the majority of lncRNAs in melanoma are unknown. The present study investigated the role of long intergenic non-protein coding RNA p53 induced transcript (LINC-PINT) in melanoma. In the present study, quantitative PCR was used to detect gene expression, overexpression experiments were performed to analyze gene interactions and CCK-8 assays were used to analyze cell proliferation. LINC-PINT was downregulated, while BRAF-activated non-coding RNA (BANCR) was upregulated in melanoma tissues compared with normal adjacent tissues. Expression levels of LINC-PINT decreased, while expression levels of BANCR increased with increasing tumor thickness. The expression levels of LINC-PINT and BANCR were inversely associated in melanoma tissues but not in healthy adjacent tissue. LINC-PINT overexpression downregulated BANCR expression in melanoma cells, while BANCR overexpression did not significantly affect LINC-PINT expression. LINC-PINT overexpression inhibited melanoma cell proliferation compared to controls. BANCR overexpression attenuated the effects of LINC-PINT overexpression. The present study revealed that lncRNA LINC-PINT is downregulated in melanoma and may regulate melanoma cell proliferation by downregulating lncRNA BANCR.
PubMed: 31452772
DOI: 10.3892/ol.2019.10631 -
OncoTargets and Therapy 2019Renal cell carcinoma (RCC) is one of the most common types of urological malignant tumors. Despite recent advances in diagnosis and management of RCC, its prognosis...
Renal cell carcinoma (RCC) is one of the most common types of urological malignant tumors. Despite recent advances in diagnosis and management of RCC, its prognosis remains poor. Emerging evidence has shown that long noncoding RNAs (lncRNAs) play crucial regulatory roles in cancer biology. The most abundant transcript of long intergenic non-protein coding RNA p53 induced transcript () in clear cell RCC (ccRCC) was determined by RT-PCR. Quantitative real-time PCR was performed to examine expression in paired ccRCC samples and cell lines. The relationship of expression with clinicopathologic characteristics and clinical outcome was analyzed. The biological function of was studied by MTS and colony formation. The flow cytometry was used to analyze cell cycle distribution and apoptosis. The subcelluar fractionation and RIP assay was performed to explore the molecular mechanism of Western blotting and immunofluorescence was carried out to examine EZH2 and p53. We found that the was frequently upregulated in ccRCC samples. Furthermore, we observed that the level of depended on gender as well as on pT and TNM stage of patients with ccRCC. Moreover, patients with high expression had poor disease-free survival and overall survival. Functionally, overexpression of promoted ccRCC cell proliferation, induced cell cycle progression, and inhibited apoptosis. was primarily located in cell nuclei and interacted with EZH2. When EZH2 was knocked down in 769P and OS-RC-2 cells overexpressing , the effect of on cell proliferation, cell cycle, and apoptosis was partially reversed. Additionally, inducing p53 by doxorubicin (Dox) promoted expression. Collectively, our results provide novel insights into the important role of in ccRCC development and indicate that may serve as a valuable prognostic biomarker for ccRCC.
PubMed: 31417274
DOI: 10.2147/OTT.S202938 -
Journal of Orthopaedic Surgery and... Aug 2023Lumbar disc herniation (LDH) is a complex spinal disease, with multiple genetic polymorphisms being related to its risk. Nevertheless, the role of LINC-PINT...
BACKGROUND
Lumbar disc herniation (LDH) is a complex spinal disease, with multiple genetic polymorphisms being related to its risk. Nevertheless, the role of LINC-PINT polymorphisms in LDH risk has remained unknown. Therefore, this study aimed to investigate the association between LINC-PINT polymorphisms and LDH risk.
METHODS
DNA was extracted from 504 LDH patients and 500 healthy controls. Three single nucleotide polymorphisms (SNPs) in LINC-PINT were selected and genotyped using Agena MassARRAY. We used logistic regression analysis to calculate odds ratios (ORs) and 95% confidence intervals (95% CIs) under multiple genetic models to evaluate the association between LINC-PINT polymorphisms and LDH risk. Haploview 4.2 and SNPStats software were used to evaluate the linkage strength of SNPs and the correlation between haplotypes and LDH risk. The impact of SNP-SNP interactions on LDH risk was analyzed using multi-factor dimensionality reduction (MDR).
RESULTS
Results showed that rs157916 (G vs. A: OR = 1.23, FDR-p = 0.029) and rs7801029 (G vs. C: OR = 1.39, FDR-p = 0.006; GG vs. CC: OR = 2.34, FDR-p = 0.038; recessive: OR = 2.13, FDR-p = 0.045; additive: OR = 1.39, FDR-p = 0.030) were associated with an increased risk of LDH. Furthermore, LINC-PINT rs157916 and rs780129 were found to be significantly associated with LDH risk in males. The "GGG" haplotype was associated with increased LDH risk (OR = 1.41, FDR-p = 0.006). MDR analysis indicated that the interaction between rs7801029 and rs16873842 was associated with an increased risk of LDH (OR = 1.47, p = 0.004). Additionally, there were significant differences in C-reactive protein levels among different genotypes of rs157916 and rs780129 (p < 0.05).
CONCLUSION
This study suggests that LINC-PINT gene polymorphisms (rs157916 and rs7801029) are considered risk factors for LDH in the Chinese Han population and provide a scientific basis for early screening, prevention, and diagnosis of LDH.
Topics: Humans; Male; Case-Control Studies; China; East Asian People; Gene Frequency; Genetic Predisposition to Disease; Intervertebral Disc Displacement; Lumbar Vertebrae; Polymorphism, Single Nucleotide; RNA, Long Noncoding
PubMed: 37553573
DOI: 10.1186/s13018-023-04052-5