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The Journal of Toxicological Sciences Dec 2003Although the patch test for visual skin observation is widely used clinically, it does not allow the mechanisms of side effects to be assessed. In this study, we... (Comparative Study)
Comparative Study
Although the patch test for visual skin observation is widely used clinically, it does not allow the mechanisms of side effects to be assessed. In this study, we examined poultice-type KP801 and tape-type KP-T patches containing ketoprofen. The parameters to measure side effects on skin were peeling intensity, amount of stripped stratum corneum, skin moisture and redness of skin color under various mechanical conditions. Since the amount of stripped stratum corneum with the tape-type KP-T patch was higher than with the poultice-type KP801 patch, the bio-adhesive strength of the latter was concluded to be lower. A clear relationship exists between the amount of stripped stratum corneum and skin moisture after tape-type patch removal, but this was not found with the poultice-type patch because of its hydration effects. Peeling intensity, one parameter to predict pain at the time of patch removal, was higher with the KP-T. As for mechanical conditions, when the patch is removed, it is important to remove it as slowly as possible and horizontally, and to avoid any rise in skin temperature. Finally, when a patch is applied to a region with little skin moisture, the amount of stripped stratum corneum may increase accordingly.
Topics: Administration, Cutaneous; Adult; Anti-Inflammatory Agents, Non-Steroidal; Delayed-Action Preparations; Human Experimentation; Humans; Ketoprofen; Male; Middle Aged; Patch Tests; Skin Irritancy Tests
PubMed: 14746345
DOI: 10.2131/jts.28.415 -
Pharmacognosy Magazine Oct 2015Bergia suffruticosa (Delile) Fenzl (Syn. Bergia odorata Edgew) (Elatinaceae family) is used traditionally to repair bones and is applied as a poultice on sores. It is...
BACKGROUND
Bergia suffruticosa (Delile) Fenzl (Syn. Bergia odorata Edgew) (Elatinaceae family) is used traditionally to repair bones and is applied as a poultice on sores. It is also used for stomach troubles and as an antidote to scorpion stings. So far, very little scientific work has been reported to validate its ethnomedical uses in the alleviation of pain, bone repair, etc.
OBJECTIVE
This study was designed to explore the anti-inflammatory and anti-implantation potential of n-hexane extract of B. suffruticosa whole plant in mice along with identification of its chemical constituents.
MATERIALS AND METHODS
n-Hexane extract of B. suffruticosa whole plant was screened for acute and chronic anti-inflammatory activity followed by an anti-estrogenic activity. Eventually, n-hexane extract was tested for anti-implantation activity by exploiting markers of uterine receptivity, lipid peroxidation, and superoxide enzyme activity. The extract was administered orally at a dose of 100 mg/kg body weight in each study.
RESULTS
Thin layer chromatography fingerprint profile of n-hexane extract revealed the presence of lupeol and β-sitosterol. The n-hexane extract reduced the edema by 80% in acute inflammation, whereas it reduced edema to 75% on the 5(th) day in chronic inflammation. The n-hexane extract reduced elevated malonaldehyde level from 6 to 2.5 nmol/g × 10(-5) and increased superoxide dismutase enzyme activity from 0 to 350 units/g in treated animals on the 5(th) day of pregnancy. Moreover, extract decreased uterine weight from 0.33 to 0.2 g in estradiol treated animals.
CONCLUSION
These results indicate that n-hexane extract of B. suffruticosa is having potent anti-inflammatory, anti-estrogenic, and anti-implantation activity. This is the first report of all the pharmacological activities of B. suffruticosa mentioned above.
SUMMARY
TLC fingerprint profile of n-hexane extract of Bergia suffruticosa whole plant revealed the presence of lupeol and β-sitosteroln-Hexane extract showed in vivo anti-inflammatory activity in both acute and chronic model of inflammation in ratsn-Hexane extract possess significant anti-estrogenic activityn-Hexane extract altered the levels superoxide anion radical and superoxide dismutase enzyme activity during the blastocyst implantationAnti-implantation activity of n-hexane extract is attributed to its anti-inflammatory and anti-estrogenic potential. Abbreviations used: TLC: Thin layer chromatography; LPO: Lipid peroxidation; SOD: Superoxide dismutase; B. suffruticosa: Bergia suffruticosa; TNF-α: Tumor necrosis factor-α; NO: Nitric oxide; IL-1: Interleukin-1; LIF: Leukemia inhibitory factor; CSF-1: Colony-stimulating factor; COX: Cyclooxygenase; SDS: Sodium dodecyl sulfate; IAEC: Animal House Ethics Committee; CPCSEA: Committee for the Purpose of Control and Supervision of Experiments on Animals; HBSS: Hank's balanced salt solution; MDA: Malonaldehyde; and TBA: Thiobarbituric acid.
PubMed: 26929574
DOI: 10.4103/0973-1296.168973 -
Evidence-based Complementary and... 2019Wounds, burns, cuts, and scarring may cause a serious problem for human health if left untreated, and medicinal plants are identified as potentially useful for wound...
Wounds, burns, cuts, and scarring may cause a serious problem for human health if left untreated, and medicinal plants are identified as potentially useful for wound healing. Therefore, the study focused on ethnophytotherapy practices for wound healing from an unexplored area, Pakistan. Ethnophytotherapeutic information was collected through well-planned questionnaire and interview methods by targeting 80 informants (70 males and 10 females), in the study area. Data was analyzed through quantitative tools like use value (UV) and credibility level (CL). A total of forty wound healing plant species, belonging to twenty-nine families, were being used in forty-six recipes. Herbs constitute (35%), shrubs (30%), trees (30%), and climbers (5%) in the treatment of multiple human injuries. For remedies preparations, leaves were most frequently utilized (52%) followed by whole plant, flowers, twigs, roots, bulb, bark, rhizome, resin, oil, leaf gel, latex, gum, and creeper. The most form of herbal preparation was powder (34.7%) and poultice (32.6%), followed by decoction, bandaged and crushed, in which 40% internally and 60 % externally applied. The drugs from these plants seem to be widely used to cure wounds: , , , , and with higher use values (0.75). Local people are still connected with ethnophytotherapies practices for curing wounds for several reasons. This ethnomedicine and the wound healing plants are under severe threats; thus conservation must be considered. Further research should be directed towards implementing pharmacological activity on these invaluable botanical drugs.
PubMed: 31379961
DOI: 10.1155/2019/4591675 -
Ayu 2019(low back pain) is a condition where low back is afflicted either with or ( involved with the toxins released due to altered digestion and metabolism) and present...
BACKGROUND
(low back pain) is a condition where low back is afflicted either with or ( involved with the toxins released due to altered digestion and metabolism) and present with symptoms such as pain with stiffness. About 60%-80% population in India suffer from this condition. (poultice) is one of the (sudation) treatment modalities mentioned for . As (affected with toxins released from impaired digestion) and (without toxins) are two stages of , hence, the specific type of is required for such condition.
AIMS AND OBJECTIVE
The study was conducted to evaluate the effect of in (low back pain) as per the presentation of stages of .
MATERIALS AND METHODS
Selected patients were categorized into two groups. In group A, patients having , were given once a day till it became , then shifted to for 7 days and patients who had was used once a day for 7 days. In group B was used once a day for 7 days irrespective of stages. Subjective parameters assessed were pain in the low back, stiffness, and Oswestry Disability Index (ODI) was also used. The Mann-Whitney U test and Wilcoxon signed‑rank test were used to assess results.
RESULTS
Patients who were treated considering the and phases had 78.88% better results in relieving pain, stiffness, and in ODI change than the patients treated without considering the phases.
CONCLUSION
The present study showed significant results in both the groups, but patients treated as per stage wise treatment showed better effect in treating than the patients treated with out considering the stage.
PubMed: 33281392
DOI: 10.4103/ayu.AYU_71_18 -
International Journal of Infectious... Nov 2005Sparganosis is a zoonosis that occurs occasionally in humans. The infection is reported in many countries but is most common in eastern Asia. In Thailand, a southeast... (Review)
Review
BACKGROUND
Sparganosis is a zoonosis that occurs occasionally in humans. The infection is reported in many countries but is most common in eastern Asia. In Thailand, a southeast Asian country, the infection is sporadic.
DESIGN
In this study the clinical presentations of human sparganosis cases in Thailand were investigated by means of a literature review.
RESULTS
Reports of 34 cases of sparganosis were found. The infections were ocular (17 cases), subcutaneous (ten cases), central nervous system (five cases), auricular (one case), pulmonary (one case), intraosseous (one case) and intraperitoneal (one case). Of these 34 cases, 14 had risk behaviour reported, 12 had a history of drinking impure water, five had a history of eating frog or snake meat and two had a history of using frog or snake meat as a poultice. Some cases had more than one risk factor.
CONCLUSION
Most cases of sparganosis in Thailand presented with superficial ocular mass lesions. The major risk behaviour in Thailand is drinking water contaminated with the infective organism. Some cases of serious deep visceral sparganosis have also been reported.
Topics: Adolescent; Adult; Animals; Child; Female; Humans; Larva; Male; Middle Aged; Risk Factors; Sparganosis; Spirometra; Thailand
PubMed: 16023879
DOI: 10.1016/j.ijid.2004.08.003 -
Journal of Holistic Nursing : Official... Dec 2015This case describes a combination external treatment for "Broken Heart Syndrome" that includes a lavender footbath, massage using moor extract, and oxalis ointment to...
This case describes a combination external treatment for "Broken Heart Syndrome" that includes a lavender footbath, massage using moor extract, and oxalis ointment to the abdomen applied by an Anthroposophic nurse for a specific personality type. Lavender footbaths have been used since ancient times for relaxation and calming, while moor extract has been used medicinally in Europe since the middle ages for warmth and environmental protection. Rhythmical massage using moor extract and oxalis ointment poultice to the abdomen are part of the tradition of Anthroposophic nursing when managing stress induced by emotional and physical trauma. An elderly lady with specific characteristics diagnosed as Broken Heart Syndrome received one treatment a week for 4 weeks given by an Anthroposophic nurse at an integrative medical center. Between treatments, education was given to enable self-treatment in the home. The nursing treatments, each using lavender footbaths, moor extract massage, and oxalis ointment poultice to the abdomen, proved very effect, and no negative effects were reported. External applications need to be considered by nurses caring for specific personality types with Broken Heart Syndrome.
Topics: Aged; Anthroposophy; Anxiety Disorders; Female; Holistic Health; Holistic Nursing; Humans; Lavandula; Massage; Oils, Volatile; Plant Oils; Takotsubo Cardiomyopathy; Treatment Outcome
PubMed: 25673580
DOI: 10.1177/0898010115569883 -
Plants (Basel, Switzerland) Jan 2021Infusions and poultices prepared from the aerial parts of Kunth (Asteraceae) are widely used in Oaxaca (Mexico) for relieving painful and inflammatory complaints....
Infusions and poultices prepared from the aerial parts of Kunth (Asteraceae) are widely used in Oaxaca (Mexico) for relieving painful and inflammatory complaints. Therefore, the antinociceptive potential of an aqueous extract (31.6-316 mg/kg, p.o.) and essential oil (30-177 µg/paw, i.pl.) of the plant was assessed using the formalin test. Both preparations inhibited the formalin-induced nociception response (100-316 mg/kg and 100-177 µg/paw, respectively) during the test's second phase. Chemical analysis of the aqueous extract revealed that the major active components were chlorogenic acid (), 3,4-di--()-caffeoylquinic acid (), 3,5-di--()-caffeoylquinic acid (), 4,5-di--()-caffeoylquinic acid (), 3,5-di--()-caffeoylquinic acid methyl ester (), apigenin (), genkwanin (), acacetin (). Compounds - and are new for . A high-pressure liquid chromatographic method for quantifying chlorogenic acid () and di-caffeoylquinic acids - in the plant was developed and validated. Analyses of the essential oil and the headspace solid-phase microextraction products, via gas-chromatography-mass spectrometry, revealed that the major volatiles were β-pinene, myrcene, D-limonene, β-caryophyllene, and α-caryophyllene, which have demonstrated antinociceptive properties.
PubMed: 33429861
DOI: 10.3390/plants10010116 -
BioMed Research International 2016Houttuynia cordata (HC) (Saururaceae) has been used internally and externally as a traditional medicine and as an herbal tea for healthcare in Japan. Our recent survey...
Houttuynia cordata (HC) (Saururaceae) has been used internally and externally as a traditional medicine and as an herbal tea for healthcare in Japan. Our recent survey showed that HC poultice (HCP) prepared from smothering fresh leaves of HC had been frequently used for the treatment of purulent skin diseases with high effectiveness. Our experimental study also demonstrated that ethanol extract of HCP (eHCP) has antibacterial, antibiofilm, and anti-inflammatory effects against S. aureus which caused purulent skin diseases. In this study, we focused on novel effects of HCP against oral infectious diseases, such as periodontal disease and dental caries. We determined the antimicrobial and antibiofilm effects of water solution of HCP ethanol extract (wHCP) against important oral pathogens and investigated its cytotoxicity and anti-inflammatory effects on human oral epithelial cells. wHCP had moderate antimicrobial effects against some oral microorganisms and profound antibiofilm effects against Fusobacterium nucleatum, Streptococcus mutans, and Candida albicans. In addition, wHCP had no cytotoxic effects and could inhibit interleukin-8 and CCL20 productions by Porphyromonas gingivalis lipopolysaccharide-stimulated human oral keratinocytes. Our findings suggested that wHCP may be clinically useful for preventing oral infectious diseases as a mouthwash for oral care.
Topics: Anti-Bacterial Agents; Bacteria; Biofilms; Cell Line; Cell Survival; Dental Caries; Houttuynia; Humans; Periodontal Diseases; Plant Extracts
PubMed: 27413739
DOI: 10.1155/2016/2581876 -
Journal of Ayurveda and Integrative... 2020Guillain-Barre syndrome (GBS) is a severe acute paralytic neuropathy with rapid progression usually occurring post infections. Inspite of the active medications it is...
Guillain-Barre syndrome (GBS) is a severe acute paralytic neuropathy with rapid progression usually occurring post infections. Inspite of the active medications it is associated with severe weakness, incomplete recovery and pain. Long disease course can cause autonomic dysfunction or deterioration in general health and life threatening complications like respiratory failures. Current case was diagnosed as GBS with motor, sensory & sphincter disturbance. Ayurveda diagnosis of Sarvangavata was made and customized treatment strategy was planned. First part of Kapha pitta samrushtavata (Vatadosha associated with Kapha and Pitta dosha) and then vatahara chikitsa were followed. Treatments were Koshta shodhana (gut cleansing), Abhyanga (massage of whole body with medicated oil), Ksheera parisheka (dripping of medicated milk over body), Shastikashali panda sweda (Rubbing of medicated rice poultice over body), Anna lepa (application of medicated rice over the body), Shirotalam (trans cranial drug administration by applying medicines over scalp), Basti (trans rectal administration of medicines) and Oral medicaments. Panchakarma treatments were for 14 days followed by oral medications for next 151 days. Intervention period of 165 days showed complete recovery of all the motor, sensory & sphincter deficits however follow up of the patient was maintained for 437 days looking in to the sustainability of the outcomes.
PubMed: 30661945
DOI: 10.1016/j.jaim.2018.08.004 -
Journal of Medical Microbiology Jan 2016Discoveries associated with antibacterial activity of hydrated clays necessitate assessments of in vivo efficacy, practical use and safety. Surface properties of clays...
Discoveries associated with antibacterial activity of hydrated clays necessitate assessments of in vivo efficacy, practical use and safety. Surface properties of clays can lead to variations in the composition and abundance of bound compounds or ions, thus affecting antibacterial activity. Since exchangeable metal ions released from the clay surface are responsible for in vitro antibacterial activity, we evaluated the in vivo antibacterial efficacy of four natural clays (one illite clay, two montmorillonite clays and one kaolinite clay) and three ion-exchanged, antibacterial clays against superficial, cutaneous meticillin-resistant Staphylococcus aureus (MRSA) infections in mice. Superficial, cutaneous wounds on the back of SKH1-Elite mice were generated and subsequently infected with MRSA. Following twice daily applications of a hydrated clay poultice to infected wounds for 7 days, we observed significant differences in the in vivo antibacterial efficacy between different types of clays. The natural and ion-exchanged illite clays performed best, as measured by bacterial load, inflammatory response and gross wound morphology with significant decreases in bacterial viability and dermatitis. Topical application of kaolinite clay was the least effective, resulting in the lowest decrease in bacterial load and exhibiting severe dermatitis. These data suggest that specific types of clays may offer a complementary and integrative strategy for topically treating MRSA and other cutaneous infections. However, since natural clays exhibit in vitro antibacterial variability and vary vastly in surface chemistries, adsorptive/absorptive characteristics and structural composition, the properties and characteristics of illite clays could aid in the development of standardized and customized aluminosilicates for topical infections.
Topics: Aluminum Silicates; Animals; Anti-Bacterial Agents; Bacterial Load; Chemical Phenomena; Clay; Disease Models, Animal; Drug Resistance, Multiple, Bacterial; Female; Inflammation; Methicillin-Resistant Staphylococcus aureus; Mice; Minerals; Skin Diseases, Bacterial; Wound Infection
PubMed: 26508716
DOI: 10.1099/jmm.0.000195