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PloS One 2020In this paper, we propose a technique that can efficiently express the preservation and breakup of liquid sheets by eliminating over-preserved liquid sheets using the...
In this paper, we propose a technique that can efficiently express the preservation and breakup of liquid sheets by eliminating over-preserved liquid sheets using the motion of water particles projected onto the screen. First, we project three-dimensional water particles onto a two-dimensional screen. When multiple particles are projected onto the same pixel, we select one of the front most particles as a screened particle by comparing their depth values. Based on the anisotropic kernel and density, the motion of the screened water particles is tracked to determine whether preservation and breakup should be performed. As a result, new water particles are added or existing ones are deleted, which makes it possible to express two characteristics of particle-based fluids: preservation and breakup of liquid sheets. The proposed technique is based on particle-based fluids, which can be used to remove the over-preserved liquid sheets and thus improve the quality of liquid sheets without surface noise.
Topics: Computer Simulation; Imaging, Three-Dimensional; Models, Molecular; Preservation, Biological; Surface Properties; Water
PubMed: 32023259
DOI: 10.1371/journal.pone.0227590 -
Annals of the Royal College of Surgeons... Aug 1969
Topics: Anatomy; Animals; Gelatin; Humans; Injections; Preservation, Biological; Resins, Plant; Rubber; Staining and Labeling
PubMed: 4192561
DOI: No ID Found -
Annals of Surgery Oct 1964
Topics: Aged; Anemia; Blood Banks; Cold Temperature; Geriatrics; Hematocrit; Hemoglobinometry; Humans; Postoperative Care; Preservation, Biological; Surgical Procedures, Operative
PubMed: 14210366
DOI: 10.1097/00000658-196410000-00009 -
Cirugia Y Cirujanos 2016Tissue storage is a medical process that is in the regulation and homogenisation phase in the scientific world. The international standards require the need to ensure...
Tissue storage is a medical process that is in the regulation and homogenisation phase in the scientific world. The international standards require the need to ensure safety and efficacy of human allografts such as skin and other tissues. The activities of skin and tissues banks currently involve their recovery, processing, storage and distribution, which are positively correlated with technological and scientific advances present in current biomedical sciences. A description is presented of the operational model of Skin and Tissue Bank at INR as successful case for procurement, recovery and preservation of skin and tissues for therapeutic uses, with high safety and biological quality. The essential and standard guidelines are presented as keystones for a tissue recovery program based on scientific evidence, and within an ethical and legal framework, as well as to propose a model for complete overview of the donation of tissues and organ programs in Mexico. Finally, it concludes with essential proposals for improving the efficacy of transplantation of organs and tissue programs.
Topics: Allografts; Cryopreservation; Global Health; Guidelines as Topic; Humans; Infection Control; Mexico; Organ Transplantation; Preservation, Biological; Professional Staff Committees; Quality Control; Skin Transplantation; Tissue Banks; Tissue and Organ Procurement
PubMed: 26259741
DOI: 10.1016/j.circir.2015.06.021 -
Microbiology Spectrum Oct 2021Field studies are central to environmental microbiology and microbial ecology, because they enable studies of natural microbial communities. Metaproteomics, the study of...
Field studies are central to environmental microbiology and microbial ecology, because they enable studies of natural microbial communities. Metaproteomics, the study of protein abundances in microbial communities, allows investigators to study these communities "," which requires protein preservation directly in the field because protein abundance patterns can change rapidly after sampling. Ideally, a protein preservative for field deployment works rapidly and preserves the whole proteome, is stable in long-term storage, is nonhazardous and easy to transport, and is available at low cost. Although these requirements might be met by several protein preservatives, an assessment of their suitability under field conditions when targeted for metaproteomic analyses is currently lacking. Here, we compared the protein preservation performance of flash freezing and the preservation solution RNA using the marine gutless oligochaete Olavius algarvensis and its symbiotic microbes as a test case. In addition, we evaluated long-term RNA storage after 1 day, 1 week, and 4 weeks at room temperature (22°C to 23°C). We evaluated protein preservation using one-dimensional liquid chromatography-tandem mass spectrometry. We found that RNA and flash freezing preserved proteins equally well in terms of total numbers of identified proteins and relative abundances of individual proteins, and none of the test time points was altered, compared to time zero. Moreover, we did not find biases against specific taxonomic groups or proteins with particular biochemical properties. Based on our metaproteomic data and the logistical requirements for field deployment, we recommend RNA for protein preservation of field-collected samples targeted for metaproteomic analyses. Metaproteomics, the large-scale identification and quantification of proteins from microbial communities, provide direct insights into the phenotypes of microorganisms on the molecular level. To ensure the integrity of the metaproteomic data, samples need to be preserved immediately after sampling to avoid changes in protein abundance patterns. In laboratory setups, samples for proteomic analyses are most commonly preserved by flash freezing; however, liquid nitrogen or dry ice is often unavailable at remote field locations, due to their hazardous nature and transport restrictions. Our study shows that RNA can serve as a low-hazard, easy-to-transport alternative to flash freezing for field preservation of samples for metaproteomic analyses. We show that RNA preserves the metaproteome equally well, compared to flash freezing, and protein abundance patterns remain stable during long-term storage for at least 4 weeks at room temperature.
Topics: Animals; Annelida; Bacteria; Bacterial Physiological Phenomena; Bacterial Proteins; Phylogeny; Preservation, Biological; Proteomics; Symbiosis
PubMed: 34704828
DOI: 10.1128/Spectrum.01429-21 -
Scientific Reports Jan 2024The introduction of fish skin as a biological dressing for treating burns and wounds holds great promise, offering an alternative to existing management strategies....
The introduction of fish skin as a biological dressing for treating burns and wounds holds great promise, offering an alternative to existing management strategies. However, the risk of disease transmission is a significant concern. Therefore, this study aimed to examine how established sterilization and preservation procedures affected fish skin grafts' microbiological and histological properties for long-term usage. Lyophilization of the fish skin graft followed by rehydration in normal saline for 15 min did not change the collagen content. Furthermore, gamma irradiation of the lyophilized fish skin graft at different lengths 5, 10, and 25 KGy showed a significant reduction in microbial growth (aerobic bacteria, aerobic yeasts, and fungi) at 15- and 30 days after the irradiation. However, exposure to 10 KGy was found to be the most effective intensity among the different gamma irradiation lengths since it preserved the collagen fiber content and intensity in the lyophilized fish skin grafts at 15- and 30 days after the irradiation. These findings provide efficient preservation and sterilization methods for long-term usage of the fresh Tilapia skin grafts used for biological dressings.
Topics: Animals; Skin Transplantation; Preservation, Biological; Freeze Drying; Collagen; Fishes; Sterilization; Ichthyosis, Lamellar
PubMed: 38218988
DOI: 10.1038/s41598-024-51608-4 -
Evaluation of formulations to improve SARS-CoV-2 viability and thermostability after lyophilisation.Journal of Virological Methods Nov 2021In the context of the COVID-19 pandemic, virus collections such as EVA-GLOBAL play a key role in the supply of viruses and related products for research. Freeze-drying...
In the context of the COVID-19 pandemic, virus collections such as EVA-GLOBAL play a key role in the supply of viruses and related products for research. Freeze-drying techniques for viruses represent a method of choice for the preservation of strains and their distribution without the need for a demanding cold chain. Here, we describe an optimised lyophilisation protocol usable for SARS-CoV-2 strains that improves preservation and thermostability. We show that sucrose used as an adjuvant represents a simple and efficient stabilizer providing increased protection for long-term preservation and shipment of the virus under different climatic conditions.
Topics: COVID-19; Freeze Drying; Humans; Pandemics; Preservation, Biological; SARS-CoV-2
PubMed: 34363874
DOI: 10.1016/j.jviromet.2021.114252 -
Fertility and Sterility Sep 1978
Topics: Female; Freezing; Humans; Insemination, Artificial; Male; Preservation, Biological; Spermatozoa
PubMed: 710607
DOI: No ID Found -
PloS One 2022Organic molecules preserved in fossils provide a wealth of new information about ancient life. The discovery of almost unaltered complex organic molecules in...
Organic molecules preserved in fossils provide a wealth of new information about ancient life. The discovery of almost unaltered complex organic molecules in well-preserved fossils raise the question of how common such occurrences are in the fossil record, how to differentiate between endogenous and exogenous sources for the organic matter and what promotes such preservation. The aim of this study was the in-situ analysis of a well-preserved vertebrate fossil from 48 Ma Eocene sediments in the Messel pit, Germany for preservation of complex biomolecules. The fossil was characterized using a variety of techniques including time-of-flight secondary ion mass spectrometry (ToF-SIMS), scanning electron microscopy/energy dispersive x-ray spectroscopy (SEM/EDX), x-ray diffraction (XRD) and Raman spectroscopy. A suite of organic molecules was detected, including porphyrins, which given the context of the detected signal are most probably diagenetically altered heme originating from the fossil though a microbial contribution cannot be completely ruled out. Diagenetic changes to the porphyrin structure were observed that included the exchange of the central iron by nickel. Further analyses on the geochemistry of the fossil and surrounding sediments showed presence of pyrite and aluminosilicates, most likely clay. In addition, a carbonate and calcium phosphate dominated crust has formed around the fossil. This suggests that several different processes are involved in the preservation of the fossil and the organic molecules associated with it. Similar processes seem to have also been involved in preservation of heme in fossils from other localities.
Topics: Animals; Fossils; Heme; Porphyrins; Preservation, Biological; Vertebrates
PubMed: 35767560
DOI: 10.1371/journal.pone.0269568 -
Annals of Anatomy = Anatomischer... Jul 2021In medical training and research fresh human tissue is often replaced by preserved human or fresh animal tissue, due to availability and ethical reasons. Newer...
BACKGROUND
In medical training and research fresh human tissue is often replaced by preserved human or fresh animal tissue, due to availability and ethical reasons. Newer preservation approaches, such as the Thiel method, promise more realistic mechanical properties than conventional formaldehyde fixation. Concerning animal substitute material, porcine and bovine tissue is often chosen, as it is easily obtainable and certain similarity to human tissue is assumed. However, it has not been thoroughly investigated how Thiel preservation changes non-linear and viscoelastic behaviour of soft organ tissues. Furthermore, differences in these properties between animal tissue and human tissue have not been previously corroborated.
METHODS
We conducted ramp and relaxation tensile tests on fresh human and Thiel preserved hepatic tissue, extracting strain-specific elastic moduli, and viscoelastic properties. The results for fresh human liver were then compared to corresponding results for Thiel preserved liver, as well as previously published results for porcine and bovine liver.
RESULTS
Our results showed that Thiel preservation seems to be associated with increased stiffness as well as decreased viscoelastic damping behaviour. Porcine liver was stiffer than human liver with similar viscoelastic properties. Bovine liver exhibited similar stiffness as human liver, however lower viscoelastic damping.
CONCLUSIONS
The differences between human and animal liver tissue, concerning their mechanical properties, can be explained by their characteristic histology. Changes in mechanical properties due to Thiel preservation might stem from altered protein cross-linking and dehydration. The results illustrate that appropriate materials for medical training systems must be selected based on which mechanical properties are relevant for the respective application.
Topics: Animals; Cattle; Elastic Modulus; Formaldehyde; Humans; Liver; Preservation, Biological; Stress, Mechanical; Swine
PubMed: 33689839
DOI: 10.1016/j.aanat.2021.151717