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Acta Stomatologica Croatica Sep 2017To determine the percentage of persistant apical lesions positive for bacterial nucleic acids, to detect microorganisms difficult to cultivate in persistant apical...
OBJECTIVES
To determine the percentage of persistant apical lesions positive for bacterial nucleic acids, to detect microorganisms difficult to cultivate in persistant apical lesions by PCR and relate them to endodontic failure, clinical symptoms and diabetes mellitus.
MATERIALS AND METHODS
The samples of persistent apical lesions were collected during apicoectomy. Bacterial ubiquitous primer 16S rRNA was used to detect 16S ribosomal RNA in 36 samples. A species-specific PCR was performed with primers targeted to the bacterial 16S rRNA genes of , , and .
RESULTS
Six samples (16.67%) were positive for bacterial ribosomal RNA. was detected in three samples. and were detected in one sample each. The prevalence of infection of such lesions with , and is low.
CONSLUSION
The study we conducted gave insufficient data about extraradicular infection and its connection with diabetes mellitus and clinical symptoms.
CONCLUSIONS
Apical lesions persisting after endodontic treatment could harbor microorganisms other than and species.
PubMed: 29225362
DOI: 10.15644/asc51/3/5 -
Antimicrobial Agents and Chemotherapy Nov 2001The AB Biodisk Etest showed that 106 (100%) and 98 (92%) isolates of Eikenella corrodens were susceptible to amoxicillin and tetracycline, respectively. Twenty-three...
The AB Biodisk Etest showed that 106 (100%) and 98 (92%) isolates of Eikenella corrodens were susceptible to amoxicillin and tetracycline, respectively. Twenty-three (68%) Prevotella intermedia isolates and 14 (67%) Prevotella nigrescens isolates were susceptible to amoxicillin. Seventy-nine percent of the P. intermedia isolates and 67% of the P. nigrescens isolates were susceptible to tetracycline. A higher percentage of beta-lactamase-producing isolates of P. intermedia and P. nigrescens were identified with selective agar containing amoxicillin than with nonselective agar.
Topics: Amoxicillin; Anti-Bacterial Agents; Culture Media; Eikenella corrodens; Humans; Microbial Sensitivity Tests; Penicillins; Periodontitis; Prevotella; Prevotella intermedia; Tetracycline
PubMed: 11600394
DOI: 10.1128/AAC.45.11.3253-3255.2001 -
Journal of Clinical Microbiology Apr 1999Primers were designed from 16S rRNA sequences of Prevotella intermedia sensu stricto and Prevotella nigrescens and were used to discriminate these two species by PCR.... (Comparative Study)
Comparative Study
Primers were designed from 16S rRNA sequences of Prevotella intermedia sensu stricto and Prevotella nigrescens and were used to discriminate these two species by PCR. The results were compared with those from the PCR technique using primers designed from arbitrarily primed PCR products by Guillot and Mouton (E. Guillot and C. Mouton, J. Clin. Microbiol. 35:1876-1882, 1997). The specificities of both assays were studied by using P. intermedia ATCC 25611, P. nigrescens ATCC 33563, 174 clinical isolates of P. intermedia sensu lato, and 59 reference strains and 58 clinical isolates of other Prevotella species and/or common oral flora. In addition, the usefulness and reliability of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in the differentiation of the two species were examined by comparing the results with those from PCR assays. The controversial lipase test for distinguishing these species was also carried out. Unambiguous differentiation was made by both PCR assays, and the results matched each other. The SDS-PAGE assay was found to misidentify a few strains tested, compared with the results of PCR assays. The lipase test was positive for both species, including the reference strains of P. intermedia and P. nigrescens. We conclude that both PCR assays are simple, rapid, reliable, and specific methods which could be used in clinical studies and that the lipase test is not valuable in the differentiation. The reliable discrimination of the two species by SDS-PAGE is questionable.
Topics: Bacterial Typing Techniques; Base Sequence; DNA Primers; Electrophoresis, Polyacrylamide Gel; Evaluation Studies as Topic; Humans; Polymerase Chain Reaction; Prevotella; Prevotella intermedia; RNA, Bacterial; RNA, Ribosomal, 16S; Reproducibility of Results; Sodium Dodecyl Sulfate; Species Specificity
PubMed: 10074526
DOI: 10.1128/JCM.37.4.1057-1061.1999 -
NPJ Biofilms and Microbiomes 2015Periodontitis is the most prevalent inflammatory disease worldwide and is caused by a dysbiotic subgingival biofilm. Here we used metatranscriptomics to determine the...
BACKGROUND/OBJECTIVES
Periodontitis is the most prevalent inflammatory disease worldwide and is caused by a dysbiotic subgingival biofilm. Here we used metatranscriptomics to determine the functional shift from health to periodontitis, the response of individual species to dysbiosis and to discover biomarkers.
METHODS
Sixteen individuals were studied, from which six were diagnosed with chronic periodontitis. Illumina sequencing of the total messenger RNA (mRNA) yielded ~42 million reads per sample. A total of 324 human oral taxon phylotypes and 366,055 open reading frames from the HOMD database reference genomes were detected.
RESULTS
The transcriptionally active community shifted from Bacilli and Actinobacteria in health to Bacteroidia, Deltaproteobacteria, Spirochaetes and Synergistetes in periodontitis. Clusters of orthologous groups (COGs) related to carbohydrate transport and catabolism dominated in health, whereas protein degradation and amino acid catabolism dominated in disease. The LEfSe, random forest and support vector machine methods were applied to the 2,000 most highly expressed genes and discovered the three best functional biomarkers, namely haem binding protein HmuY from , flagellar filament core protein FlaB3 from , and repeat protein of unknown function from They predicted the diagnosis correctly for 14 from 16 individuals, and when applied to an independent study misclassified one out of six subjects only. shifted from commensalism to virulence by upregulating the expression of metalloproteases and the haem transporter. Expression of genes for the synthesis of the cytotoxic short-chain fatty acid butyrate was observed by under all conditions. Four additional species contributed to butyrate synthesis in periodontitis and they used an additional pathway.
CONCLUSION
Gene biomarkers of periodontitis are highly predictive. The pro-inflammatory role of is not related to butyrate synthesis.
PubMed: 28721234
DOI: 10.1038/npjbiofilms.2015.17 -
FEMS Immunology and Medical Microbiology May 1998The antimicrobial effect of lactoferrin (apoLf) on the oral, black-pigmented anaerobes Porphyromonas gingivalis, Prevotella intermedia and P. nitrescens has been...
The antimicrobial effect of lactoferrin (apoLf) on the oral, black-pigmented anaerobes Porphyromonas gingivalis, Prevotella intermedia and P. nitrescens has been studied. ApoLf did not kill any of these species but it did inhibit the growth of P. gingivalis, while iron-saturated Lf (FeLf) had no effect. The other two species were unaffected by apoLf. This growth inhibitory effect of apoLf could not be explained on the basis of chelation of inorganic iron, since growth of P. gingivalis occurred in the presence of ethylenediamine di-o-hydroxyphenylacetic acid provided haemin was added. Both apoLf and FeLf reduced haemin uptake by all three species and caused the release of cell-bound haemin in a dose-dependent manner. In addition, haemin reduced the binding of both apoLf and FeLf to P. intermedia and P. nigrescens but stimulated the binding of Lf by P. gingivalis. These data suggest that Lf forms complexes with haemin in solution and competes for the binding of haemin to certain cell receptors, possibly lipopolysaccharides, but this is not sufficient to inhibit growth of the bacteria. P. gingivalis appears to bind Lf-haemin complexes, as well as haemin alone, which may facilitate access of the Lf to the outer and cytoplasmic membranes of P. gingivalis, so disrupting function.
Topics: Colony Count, Microbial; Hemin; Iron Chelating Agents; Lactoferrin; Porphyromonas gingivalis; Prevotella; Prevotella intermedia
PubMed: 9657318
DOI: 10.1111/j.1574-695X.1998.tb01146.x -
PloS One 2016To assess and compare salivary periodontopathic bacteria between groups of Down syndrome and non-Down syndrome children and adolescents.
OBJECTIVE
To assess and compare salivary periodontopathic bacteria between groups of Down syndrome and non-Down syndrome children and adolescents.
MATERIALS AND METHODS
This study included a sample of 30 Down syndrome children and adolescents (G-DS) and 30 age- and sex-matched non-Down syndrome subjects (G-ND). Clinical examination determined the gingival bleeding index (GBI) and plaque index. Unstimulated whole saliva samples were collected from all participants. The fluorescence in situ hybridization (FISH) technique identified the presence and density of eight periodontopathic bacteria in saliva. The statistical analysis included chi-square and Mann-Whitney U tests.
RESULTS
In the G-DS group, bleeding on probing was more frequent (p = 0.037) and higher densities of Campylobacter rectus (p = 0.013), Porphyromonas gingivalis (p = 0.025), Treponema denticola (p = 0.026), Fusobacterium nucleatum (p = 0.013), Prevotella intermedia (p = 0.001) and Prevotella nigrescens (p = 0.008) were observed. Besides, in the G-DS, the densities of bacteria from the orange complex were significantly higher in the age group 3-7 years for F. nucleatum (p = 0.029), P. intermedia (p = 0.001) and P. nigrescens (p = 0.006). C. rectus was higher in the age group 8-12 years (p = 0.045).
CONCLUSION
The results showed that children and adolescents with Down syndrome have higher susceptibility to periodontal disease and number of periodontopathic bacteria.
Topics: Campylobacter rectus; Case-Control Studies; Child; Child, Preschool; DNA, Bacterial; Dental Plaque Index; Down Syndrome; Female; Fusobacterium nucleatum; Gram-Negative Bacteria; Humans; In Situ Hybridization, Fluorescence; Male; Periodontal Diseases; Periodontal Index; Porphyromonas gingivalis; Prevotella intermedia; Saliva; Treponema denticola
PubMed: 27727287
DOI: 10.1371/journal.pone.0162988 -
Microbiology (Reading, England) Jul 2003The haem pigment of Porphyromonas gingivalis is composed of micro -oxo bishaem, [Fe(III)PPIX](2)O, but the nature of that generated by Prevotella species has not been...
The haem pigment of Porphyromonas gingivalis is composed of micro -oxo bishaem, [Fe(III)PPIX](2)O, but the nature of that generated by Prevotella species has not been established. Mössbauer, Raman and UV-visible spectrophotometry were used to characterize the haem pigment of Prevotella intermedia and Prevotella nigrescens. Mössbauer and Raman spectroscopy revealed the major haem species to be monomeric iron protoporphyrin IX, Fe(III)PPIX.OH (haematin). The terminal growth pH of both species on blood agar was between 5.8 and 6.0, which favours the formation and maintenance of monomeric Fe(III)PPIX.OH. Incubation of Pr. nigrescens and Pr. intermedia with oxyhaemoglobin at pH 6.5 resulted in formation of aquomethaemoglobin which was degraded to generate Fe(III)PPIX.OH which in turn became cell-associated, whilst incubation at pH 7.5 resulted in formation of [Fe(III)PPIX](2)O. It is concluded that both Prevotella species degrade oxyhaemoglobin to form [Fe(III)PPIX](2)O as an intermediate, which is converted to Fe(III)PPIX.OH through a depression in pH. The low pH encourages cell-surface deposition of insoluble Fe(III)PPIX.OH which would act as a barrier against oxygen and reactive oxygen species, and also protect against H(2)O(2) through its inherent catalase activity.
Topics: Animals; Heme; Horses; Humans; Hydrogen Peroxide; Hydrogen-Ion Concentration; In Vitro Techniques; Oxyhemoglobins; Pigments, Biological; Prevotella; Prevotella intermedia; Protoporphyrins; Spectrophotometry; Spectroscopy, Mossbauer; Spectrum Analysis, Raman
PubMed: 12855722
DOI: 10.1099/mic.0.26258-0 -
Veterinarni Medicina Feb 2023Black-pigmented bacteria are one of the neglected species to cause periodontal disease in cats, and they are also zoonotic agents that pose an infection risk...
Black-pigmented bacteria are one of the neglected species to cause periodontal disease in cats, and they are also zoonotic agents that pose an infection risk to humans. In this study, we aimed to determine the presence of , and in the oral microbiota of pet and stray cats. Dental swab samples were taken from 25 pet cats and 25 stray cats with symptoms of periodontal disease and then investigated by multiplex polymerase chain reaction using 16S rRNA species-specific primers. As a result of the multiplex PCR analysis, 3/25 (12%), 1/25 (4%), + 7/25 (28%), + 1/25 (4%), + 1/25 (4%), and + + 2/25 (8%) were molecularly typed in the pet cats. In addition, 1/25 (4%) of and 21/25 (84%) of + were typed in the stray cats. In 10/25 (40%) pet and 3/25 (12%) stray cat samples, no bacteria were detected by molecular typing. In summary, the results provide strong evidence that black-pigmented zoonotic pathogens are associated with cat periodontal disease.
PubMed: 38332760
DOI: 10.17221/59/2022-VETMED -
Scientific Reports Jul 2021A growing body of evidence supports an important role for alterations in the brain-gut-microbiome axis in the aetiology of depression and other psychiatric disorders....
A growing body of evidence supports an important role for alterations in the brain-gut-microbiome axis in the aetiology of depression and other psychiatric disorders. The potential role of the oral microbiome in mental health has received little attention, even though it is one of the most diverse microbiomes in the body and oral dysbiosis has been linked to systemic diseases with an underlying inflammatory aetiology. This study examines the structure and composition of the salivary microbiome for the first time in young adults who met the DSM-IV criteria for depression (n = 40) and matched controls (n = 43) using 16S rRNA gene-based next generation sequencing. Subtle but significant differences in alpha and beta diversity of the salivary microbiome were observed, with clear separation of depressed and healthy control cohorts into distinct clusters. A total of 21 bacterial taxa were found to be differentially abundant in the depressed cohort, including increased Neisseria spp. and Prevotella nigrescens, while 19 taxa had a decreased abundance. In this preliminary study we have shown that the composition of the oral microbiome is associated with depression in young adults. Further studies are now warranted, particuarly investigations into whether such shifts play any role in the underling aetiology of depression.
Topics: Adolescent; Adult; Age Factors; Bacteria; Biodiversity; Case-Control Studies; Depression; Female; Host Microbial Interactions; Humans; Male; Metagenome; Metagenomics; Microbiota; Mouth; Saliva; Young Adult
PubMed: 34294835
DOI: 10.1038/s41598-021-94498-6 -
Brazilian Oral Research 2015This study aimed to determine the presence of Prevotella strains and genes associated with resistance to lactamics in different oral niches from patients with/without...
This study aimed to determine the presence of Prevotella strains and genes associated with resistance to lactamics in different oral niches from patients with/without primary endodontic infections. Saliva (S) and supragingival biofilm (SB) were collected from three patient groups: Group I - no endodontic infection (n = 15); Group II - acute endodontic infection (n = 12); and Group III - chronic endodontic infection (n = 15). Root canal (RC) samples were collected from Groups II and III. The presence of P. intermedia, P nigrescens, P. tannerae and cfxA/cfxA2 gene was assessed by PCR. The cfxA/cfxA2 gene was not detected in all environments within the same patient. The cfxA/cfxA2 gene was present in 23.81% of S samples, 28.57% of SB samples, and 7.41% of RC samples. Prevotella species were detected in 53.97%, 47.62% and 34.56% of the S, SB, and RC samples, respectively. P. intermedia had a high frequency in saliva samples from Group 3. Saliva samples from Group 1 had higher detection rates of P. nigrescens than did Groups 2 and 3. Patients without endodontic disease had high frequencies of P. nigrescens in the SB samples. The presence or absence of spontaneous symptoms was not related to the detection rates for resistance genes in the RC samples. Saliva, supragingival biofilm and root canals can harbor resistant bacteria. The presence of symptomatology did not increase the presence of the cfxA/cfxA2 gene in the supragingival biofilm and inside root canals.
Topics: Adult; Aged; Biofilms; Chi-Square Distribution; DNA, Bacterial; Dental Pulp Cavity; Female; Gingiva; Humans; Male; Middle Aged; Polymerase Chain Reaction; Prevotella; Saliva; Sequence Analysis, DNA; Young Adult; beta-Lactam Resistance; beta-Lactamases
PubMed: 25789508
DOI: 10.1590/1807-3107BOR-2015.vol29.0052