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Veterinary World Jul 2020is one of the emerging gram-negative bacteria, which associated with foodborne illness. Researches affirmed that distinguish between and are difficult, as they are...
BACKGROUND AND AIM
is one of the emerging gram-negative bacteria, which associated with foodborne illness. Researches affirmed that distinguish between and are difficult, as they are phylogenetic related. The evolution of multidrug resistance of strains gained more concern for recognition of the pathogen which supports in controlling the disease and minify its threat. This study sought to find a reliable tool for the identification of , isolated from chicken product samples, and assessed the resistance profile of . using antibiogram sensitivity tests.
MATERIALS AND METHODS
Forty samples of chicken products were collected between January and September 2019 from different markets in Alexandria Governorate, Egypt. The products included nuggets, strips, burgers, luncheon meats, pane, frankfurters, and minced chicken meat. The samples were transferred to the Reference Laboratory. The samples were subjected to isolation, biochemical reaction testing, phenotypic system analytical profile index (API) E20, and a detection of antimicrobial susceptibility test. Phenotypic identification was confirmed through matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).
RESULTS
Thirty-three bacterial isolates (82.50%) out of 40 samples were isolated into pure cultures from the chicken samples. Three isolates (9.09%) were positive for , while 30 isolates (90.91%) exhibited growth characters for different pathogens (, , , and ). The isolates of were resistant to five types of antibiotics and sensitive to two types of antibiotics.
CONCLUSION
This study reported the first case of found in chicken products in Egypt. Phenotypic system API 20E and MALDI-TOF MS were found to be reliable tools for confirming the diagnosis of a. As it provides rapid identification with high sensitivity and specificity for , which often do not require a molecular procedure for confirmation.
PubMed: 32848326
DOI: 10.14202/vetworld.2020.1473-1479 -
Molecules (Basel, Switzerland) Aug 2023Biological methods are currently the most commonly used methods for removing hazardous substances from land. This research work focuses on the remediation of...
Biological methods are currently the most commonly used methods for removing hazardous substances from land. This research work focuses on the remediation of oil-contaminated land. The biodegradation of aliphatic hydrocarbons and PAHs as a result of inoculation with biopreparations B1 and B2 was investigated. Biopreparation B1 was developed on the basis of autochthonous bacteria, consisting of strains sp. IN118, sp. IN101, IN53, IN119, IN113 and sp. IN109, whereas biopreparation B2 was enriched with fungi, such as , , sp., , . As a result of biodegradation tests conducted under ex situ conditions for soil inoculated with biopreparation B1, the concentrations of TPH and PAH were reduced by 31.85% and 27.41%, respectively. Soil inoculation with biopreparation B2 turned out to be more effective, as a result of which the concentration of TPH was reduced by 41.67% and PAH by 34.73%. Another issue was the phytoremediation of the pre-treated G6-3B2 soil with the use of . The tests were carried out in three systems (system 1-soil G6-3B2 + ; system 2-soil G6-3B2 + biopreparation B2 + ; system 3-soil G6-3B2 + biopreparation B2 with γ-PGA + ) for 6 months. The highest degree of TPH and PAH reduction was obtained in system 3, amounting to 65.35% and 60.80%, respectively. The lowest phytoremediation efficiency was recorded in the non-inoculated system 1, where the concentration of TPH was reduced by 22.80% and PAH by 18.48%. Toxicological tests carried out using Phytotoxkit, Ostracodtoxkit and Microtox Solid Phase tests confirmed the effectiveness of remediation procedures and showed a correlation between the concentration of petroleum hydrocarbons in the soil and its toxicity. The results obtained during the research indicate the great potential of bioremediation practices with the use of microbial biopreparations and in the treatment of soils contaminated with petroleum hydrocarbons.
Topics: Zea mays; Biodegradation, Environmental; Hazardous Substances; Actinomycetales; Enterobacteriaceae
PubMed: 37630356
DOI: 10.3390/molecules28166104 -
Molecular Plant-microbe Interactions :... Feb 2017Mechanical damage caused by insect feeding along with components present in insect saliva and oral secretions are known to induce jasmonic acid-mediated defense...
Mechanical damage caused by insect feeding along with components present in insect saliva and oral secretions are known to induce jasmonic acid-mediated defense responses in plants. This study investigated the effects of bacteria from oral secretions of the fall armyworm Spodoptera frugiperda on herbivore-induced defenses in tomato and maize plants. Using culture-dependent methods, we identified seven different bacterial isolates belonging to the family Enterobacteriacea from the oral secretions of field-collected caterpillars. Two isolates, Pantoea ananatis and Enterobacteriaceae-1, downregulated the activity of the plant defensive proteins polyphenol oxidase and trypsin proteinase inhibitors (trypsin PI) but upregulated peroxidase (POX) activity in tomato. A Raoultella sp. and a Klebsiella sp. downregulated POX but upregulated trypsin PI in this plant species. Conversely, all of these bacterial isolates upregulated the expression of the herbivore-induced maize proteinase inhibitor (mpi) gene in maize. Plant treatment with P. ananatis and Enterobacteriaceae-1 enhanced caterpillar growth on tomato but diminished their growth on maize plants. Our results highlight the importance of herbivore-associated microbes and their ability to mediate insect plant interactions differently in host plants fed on by the same herbivore.
Topics: Animals; Bacteria; Gastrointestinal Microbiome; Herbivory; Insect Proteins; Larva; Solanum lycopersicum; Saliva; Salivary Proteins and Peptides; Spodoptera; Weight Gain; Zea mays
PubMed: 28027025
DOI: 10.1094/MPMI-11-16-0240-R -
Evidence-based Complementary and... 2022Mobile phones have become an indispensable part of human lives for communication, education, and entertainment activities. This study aims to evaluate the diversity...
Mobile phones have become an indispensable part of human lives for communication, education, and entertainment activities. This study aims to evaluate the diversity pattern of bacterial contaminants on mobiles and to check antibiotic resistance profiles in 105 samples. The study revealed a contamination of 51% in men and 49% in women, the highest in the 21- to 30-year age group, evidencing the extreme use of mobiles by teenagers. The study observed Gram-negative bacteria (63%) versus Gram-positive bacteria (37%). Overall, Gram-negative bacterial isolates showed the highest sensitivity to antibiotic nitrofurantoin (90%) and the lowest in ampicillin (35%). Gram positive has highest incidence of sensitivity towards tigecycline (100%) and lowest in cefoxitin (20%). ESL producers were found to be 21.0% and highest being in (35%) followed by (31%). have been identified on the mobile phones for the very first time. Interestingly, some soil microbes were also isolated and unfortunately found to have some antibiotic resistance like and . The results revealed that mobiles were contaminated with multidrug-resistant (MDR) pathogens, and this study also showed that few of the saprophytic soil strains have antibiotic resistance, which can be an alarming situation that needs to be addressed.
PubMed: 35668788
DOI: 10.1155/2022/1527488 -
Microorganisms Oct 2023is an bacterium that can infect both humans and animals, while luteolin-7-O-glucoside (IOG) is a flavonoid that has broad effects on the intestinal microbiota of...
Effects of Luteolin-7-O-Glucoside on Intestinal Microbiota Dysbiosis and Drug Resistance Transmission Caused by B1645-1: Modulating the Composition of Intestinal Microbiota and Promoting the Transfer of Gene from Genus to in Mice.
is an bacterium that can infect both humans and animals, while luteolin-7-O-glucoside (IOG) is a flavonoid that has broad effects on the intestinal microbiota of healthy animals. However, current studies lack sufficient data on intestinal microbiota dysbiosis and drug resistance transmission caused by and the possible role of IOG. In this study, BALB/c mice were infected with carrying gene and treated with IOG (3 mg/kg·d and 6 mg/kg·d) to analyze the diversity of intestinal microbiota and the transfer of between bacteria. The findings indicated that B1645-1 exhibited a significant ability to enhance the / ratio and increase the relative abundance of and after 48 h, where as 6 mg/kg·d IOG had an opposite effect. Moreover, B1645-1 facilitated the emergence of drug-resistant bacteria and promoted gene transfer in , , , , , , and . was the predominant genus at 48 h. Surprisingly, 6 mg/kg·d IOG significantly inhibited the production of drug-resistant bacteria and promoted gene transfer from to at 144 h. However, the role of as a recipient for drug-resistant genes should be of more concern.
PubMed: 37894135
DOI: 10.3390/microorganisms11102477 -
Iranian Journal of Biotechnology Oct 2022Nanoparticles can be chemically, physically, or biologically synthesized. Biosynthesis of silver nanoparticles (AgNPs) utilizing microbes is a promising process due to...
BACKGROUND
Nanoparticles can be chemically, physically, or biologically synthesized. Biosynthesis of silver nanoparticles (AgNPs) utilizing microbes is a promising process due to the low toxicity and high stability of AgNPs. Here, AgNPs were fabricated by Gram-negative .
OBJECTIVES
This study aimed to assess the ability of to produce nanoparticles (NPs) and evaluate their antibacterial potential against multidrug-resistant pathogens (MDR). Additionally, the study aimed to compare the antibacterial activity of biosynthesized nanoparticles to well-known conventional antibiotics Azithromycin and Tetracycline.
MATERIALS AND METHODS
AgNPs were characterized using visual observation, UV-visible spectroscopy (UV-vis), X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR). The TEM and SEM were used to determine the size and shape of the nanoparticles. The XRD data were recorded in the 2θ ranging from 20-80° to analyze the crystalline structure of nanoparticles. The antibacterial activity was detected using a 96-well microtiter plate.
RESULTS
The UV-vis absorption recorded from the 300 - 900 nm spectrum was well defined at 420 nm, and the XRD pattern was compatible with Braggs's reflection of the silver nanocrystals. FTIR showed absorbance bands corresponding to different functional groups. TEM and SEM images showed non-uniform spherical and AgNPs of 10-80 nm. XRD data confirmed that the resultant particles are AgNPs. The AgNPs showed effective activity against multi-drug resistant (MDR) , sp., sp., , sp., , and . The AgNPs demonstrated effectiveness in lower concentrations compared to broad-spectrum antibiotics.
CONCLUSION
These data reveal that AgNP generated by was more efficient against MDR microorganisms than commercial antibiotics. However, the cytotoxicity of these nanoparticles must be further studied.
PubMed: 38344316
DOI: 10.30498/ijb.2022.298773.3121 -
Cureus Oct 2023We present a case of () infection that resulted in community-acquired pneumonia in an immunocompetent patient with an eight-week history of productive cough. This...
We present a case of () infection that resulted in community-acquired pneumonia in an immunocompetent patient with an eight-week history of productive cough. This gram-negative bacterium is typically found in the environment and has the potential to infect humans. infections in humans have been recorded in several case reports from throughout the world in recent years, usually affecting immunocompromised patients. Although is sensitive to most antibiotic groups, recent studies have revealed an increase in the infrequent acquisition of resistance genes in , such as carbapenem resistance, making this pathogen a potential emergent threat. Our patient acquired pneumonia in the absence of any underlying risk factors, making this the first case in the UK of causing community-acquired pneumonia in an immunocompetent person.
PubMed: 38021927
DOI: 10.7759/cureus.47188 -
Applied and Environmental Microbiology Jul 2002Histamine fish poisoning is caused by histamine-producing bacteria (HPB). Klebsiella pneumoniae and Klebsiella oxytoca are the best-known HPB in fish. However, 22...
Histamine fish poisoning is caused by histamine-producing bacteria (HPB). Klebsiella pneumoniae and Klebsiella oxytoca are the best-known HPB in fish. However, 22 strains of HPB from fish first identified as K. pneumoniae or K. oxytoca by commercialized systems were later correctly identified as Raoultella planticola (formerly Klebsiella planticola) by additional tests. Similarly, five strains of Raoultella ornithinolytica (formerly Klebsiella ornithinolytica) were isolated from fish as new HPB. R. planticola and R. ornithinolytica strains were equal in their histamine-producing capabilities and were determined to possess the hdc genes, encoding histidine decarboxylase. On the other hand, a collection of 61 strains of K. pneumoniae and 18 strains of K. oxytoca produced no histamine.
Topics: Animals; Blotting, Southern; Enterobacteriaceae; Feces; Fishes; Histamine; Klebsiella pneumoniae; Molecular Sequence Data; Polymerase Chain Reaction
PubMed: 12089029
DOI: 10.1128/AEM.68.7.3462-3466.2002 -
Cureus Mar 2024The case presentation discusses the clinical evaluation and treatment of a two-year-old female exhibiting symptoms such as dysuria, constipation, and foul-smelling...
The case presentation discusses the clinical evaluation and treatment of a two-year-old female exhibiting symptoms such as dysuria, constipation, and foul-smelling urine. Upon evaluation, the patient was found to be co-infected with Raoultella planticola and Escherichia coli. This co-infection poses unique challenges in diagnosis and treatment, as both pathogens may contribute to the manifestation of symptoms. The initial diagnosis of Raoultella planticola is notable, given its relatively rare occurrence and the potential for misdiagnosis. This case study contributes to our understanding of diagnosing and distinguishing symptoms at various stages of the illness, particularly in cases of co-infection. Following an initial urinalysis and urine culture confirming the presence of both pathogens, a 10-day course of antibiotics was prescribed. Subsequent examinations at Rady Children's Hospital-San Diego included kidney and abdomen imaging to rule out underlying issues. The co-infection underscores the importance of thorough diagnostic procedures and tailored treatment approaches. Additionally, it highlights the need for heightened awareness among healthcare providers regarding emerging pathogens and their potential clinical implications.
PubMed: 38686243
DOI: 10.7759/cureus.57262 -
Frontiers in Cellular and Infection... 2021Accumulative evidence suggests that gut microbiota play an important role in bone remodeling and hence bone health maintenance. This study aimed to explore the...
OBJECTIVE
Accumulative evidence suggests that gut microbiota play an important role in bone remodeling and hence bone health maintenance. This study aimed to explore the association of gut microbiota with the risk of osteoporosis and to identify potential disease-related taxa, which may be promising targets in osteoporosis prevention and treatment in the future.
METHODS
Absolute quantification 16S ribosomal RNA gene sequencing was used to detect absolute and relative abundances of gut microbiota in 44 patients with osteoporosis and 64 controls. In combination with one of our previous studies, a total of 175 samples were involved in the relative abundance analysis.
RESULTS
Compared with the controls, the patients with osteoporosis had higher absolute and relative abundances of Bacteroidetes phylum, and and genera. The absolute abundances of , , , and genera increased, and that of the genus decreased in the osteoporosis group. As for relative abundance, that of the and genera increased, whereas that of the genus decreased in the osteoporosis group. Controlling for potential confounders, the associations of , , and genera with the risk of osteoporosis did not maintain significance. Ridge regression analysis suggested that is associated with reduced bone mineral density (BMD) and T-score at lumbar spines, and is associated with increased BMD at the femoral neck. Functional predictions revealed that 10 Kyoto Encyclopedia of Genes and Genomes pathways were enriched in the osteoporosis group.
CONCLUSIONS
Gut microbiota compositions may contribute to the risk of osteoporosis. Several specific taxa and functional pathways are identified to associate with reduced bone density, thus providing epidemiologic evidence for the potential role of aberrant gut microbiota in osteoporosis pathogenesis.
Topics: Aged; China; Clostridiales; Feces; Gastrointestinal Microbiome; High-Throughput Nucleotide Sequencing; Humans; Osteoporosis; RNA, Ribosomal, 16S
PubMed: 33996619
DOI: 10.3389/fcimb.2021.630372