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The Journal of Antibiotics Oct 1985A strain of Streptomyces hygroscopicus No. J296-21 (ATCC 39150) was found to produce a complex of new antibiotics, called inosamycins, which consisted of components A,...
A strain of Streptomyces hygroscopicus No. J296-21 (ATCC 39150) was found to produce a complex of new antibiotics, called inosamycins, which consisted of components A, B, C, D and E. They are novel aminocyclitol antibiotics structurally related to neomycin, paromomycin and ribostamycin. The antibiotic complex and each component of inosamycin exhibit a broad antibacterial spectrum but they are inactive against most of the aminoglycoside-resistant organisms. The antibacterial activity of inosamycin A, the major component of the complex, is comparable to that of neomycin but its acute toxicity is significantly lower (ca. 1/3) than that of neomycin.
Topics: Aminoglycosides; Animals; Anti-Bacterial Agents; Bacteria; Bacterial Infections; Chemical Phenomena; Chemistry; Fermentation; Mice; Streptomyces
PubMed: 4066486
DOI: 10.7164/antibiotics.38.1302 -
The Journal of Antibiotics Oct 1985Structures of the new aminocyclitol antibiotics, inosamycins A, B, C, D and E, have been determined by a combination of chemical degradation and spectroscopic studies....
Structures of the new aminocyclitol antibiotics, inosamycins A, B, C, D and E, have been determined by a combination of chemical degradation and spectroscopic studies. They are structurally related to neomycin, paromomycin and ribostamycin but differ in that all the inosamycin components contain 2-deoxy-scyllo-inosamine in place of 2-deoxystreptamine in the known aminoglycoside antibiotics.
Topics: Aminoglycosides; Anti-Bacterial Agents; Chemical Phenomena; Chemistry; Magnetic Resonance Spectroscopy
PubMed: 4066487
DOI: 10.7164/antibiotics.38.1313 -
The Journal of Antibiotics Apr 1973
Topics: Amino Sugars; Anti-Bacterial Agents; Chemical Phenomena; Chemistry; Culture Media
PubMed: 4782052
DOI: No ID Found -
Antimicrobial Agents and Chemotherapy Jan 1974It was found that the 5''-hydroxy group of ribostamycin was preferentially phosphorylated by a cell-free extract from Pseudomonas aeruginosa GN 573, a clinical isolate.
It was found that the 5''-hydroxy group of ribostamycin was preferentially phosphorylated by a cell-free extract from Pseudomonas aeruginosa GN 573, a clinical isolate.
Topics: Aminoglycosides; Anti-Bacterial Agents; Cell-Free System; Chromatography, Thin Layer; Oxidative Phosphorylation; Pseudomonas aeruginosa
PubMed: 4209071
DOI: 10.1128/AAC.5.1.92 -
Association of aminoglycosidic antibiotics with the ribosomal A-site studied with Brownian dynamics.Journal of Chemical Theory and... Apr 2008Brownian dynamics methodology was applied to simulate the encounter of aminoglycosidic antibiotics with the ribosomal A-site RNA. Studied antibiotics included neamine,...
Brownian dynamics methodology was applied to simulate the encounter of aminoglycosidic antibiotics with the ribosomal A-site RNA. Studied antibiotics included neamine, neomycin, ribostamycin and paromomycin which differ in chemical structure, the number of pseudo-sugar rings and the net charge. The influence of structural, electrostatic and hydrodynamic properties of antibiotics on the kinetics of their association with the ribosomal A-site was analyzed. The computed diffusion limited rates of association are of the order of 10(10)[Formula: see text] and they weakly depend on ionic strength. Prior to binding antibiotics often slide along the RNA groove with the time scale of approximately 10 ns per base pair in case of neamine. We observed that upon forming the encounter complex aminoglycosides displace from the binding pocket up to two Mg(2+) ions.
PubMed: 19343095
DOI: 10.1021/ct700210n -
The Journal of Antibiotics Jun 1983
Topics: Anti-Bacterial Agents; Glucosamine; Magnetic Resonance Spectroscopy; Ribostamycin; Streptomyces; Structure-Activity Relationship
PubMed: 6874597
DOI: 10.7164/antibiotics.36.749 -
The Journal of Antibiotics Jan 1986Gentamicin C2 interacts cooperatively with ribosomes from a sensitive Escherichia coli strain in a multiphasic way with several classes of sites. It is shown that this...
Gentamicin C2 interacts cooperatively with ribosomes from a sensitive Escherichia coli strain in a multiphasic way with several classes of sites. It is shown that this binding is highly-dependent on Mg++ and natural endogenous polyamine concentrations. The differences observed between ribosomes from sensitive and resistant strains may be explained by the absence of specific cooperative gentamicin interactions with resistant ribosomes. The effects of other aminoglycoside antibiotics are discussed in terms of structure-activity relationships.
Topics: Aminoglycosides; Ammonia; Drug Resistance, Microbial; Escherichia coli; Gentamicins; Magnesium; Osmolar Concentration; Polyamines; Ribosomes; Ribostamycin; Spermidine; Tobramycin
PubMed: 3512503
DOI: 10.7164/antibiotics.39.136 -
Journal of Bacteriology Feb 1991Insertional mutagenesis of the Haemophilus influenzae chromosome was accomplished by a novel method employing a 2.2-kbp element, TSTE. This element, consisting of the...
Insertional mutagenesis of the Haemophilus influenzae chromosome was accomplished by a novel method employing a 2.2-kbp element, TSTE. This element, consisting of the neo gene of Tn5 flanked by Haemophilus-specific uptake sequences, was ligated to circularized chromosomal fragments before transformation into the homologous strain. Eight mutants defective in the production of haemocin were detected. This strategy provides an efficient mechanism for the insertional mutagenesis of H. influenzae.
Topics: DNA Transposable Elements; Haemophilus influenzae; Kanamycin Kinase; Mutagenesis, Insertional; Nucleic Acid Hybridization; Phosphotransferases; Ribostamycin; Transformation, Bacterial
PubMed: 1847369
DOI: 10.1128/jb.173.4.1561-1564.1991 -
Antimicrobial Agents and Chemotherapy Oct 1976A fast and accurate enzymatic estimation of the concentration of aminoglycoside antiobiotics in serum is given by aminoglycoside 4'-adenylyltransferase (synonym:...
Enzymatic adenylylation by aminoglycoside 4'-adenylytransferase and 2"-adenylyltransferase as a means of determining concentrations of aminoglycoside antibiotics in serum.
A fast and accurate enzymatic estimation of the concentration of aminoglycoside antiobiotics in serum is given by aminoglycoside 4'-adenylyltransferase (synonym: tobramycin adenylyltransferase), a new enzyme recently found in tobramycin-resistant staphylococci (P. Santanam and F. H. Kayser, J. Infect. Dis. [Suppl.], in press). This enzyme is useful in assaying the kanamycins, the butirosins, amikacin, ribostamycin and, if required, the neomycins, tobramycin, and paromomycin. In combination with the assay using aminoglycoside 2''-adenylyltransferase (synonym: gentamicin adenylyltransferase) to determine the level of gentamicin, sisomicin, and tobramycin, a convenient method to estimate a wide range of aminoglycoside antibiotics in patients' sera is described.
Topics: Aminoglycosides; Anti-Bacterial Agents; Clinical Enzyme Tests; Humans; In Vitro Techniques; Methods; Nucleotidyltransferases; Staphylococcus
PubMed: 984802
DOI: 10.1128/AAC.10.4.664 -
Isolation of an intermediate of 2-deoxystreptamine biosynthesis from a mutant of Bacillus circulans.The Journal of Antibiotics Aug 1980Eight 2-deoxystreptamine-negative (DOS-) mutants were isolated from various strains of Bacillus circulans, normally producing butirosin, xylostasin and ribostamycin....
Eight 2-deoxystreptamine-negative (DOS-) mutants were isolated from various strains of Bacillus circulans, normally producing butirosin, xylostasin and ribostamycin. These mutants were classified into two groups (converter and secretor) by the simple method of cosynthesis using agar plate culture. One of the cosynthetic pairs, strain S-11, an intermediate of DOS biosynthesis, S-11-P, was isolated. This compound was converted to butirosin (BTN) effectively by strain 236. The structure of S-11-P was considered to be (1 L or 1 D)-1,3,5/2,4-5-amino-cyclohexanetetrol, and the pathway of DOS biosynthesis is discussed here.
Topics: Anti-Bacterial Agents; Bacillus; Chemical Phenomena; Chemistry, Physical; Fermentation; Hexosamines; Mutation
PubMed: 7429985
DOI: 10.7164/antibiotics.33.824