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Scientific Reports Nov 2023Many diseases, including cancer and covid, result in altered mechanical and electric properties of the affected cells. These changes were proposed as disease markers....
Many diseases, including cancer and covid, result in altered mechanical and electric properties of the affected cells. These changes were proposed as disease markers. Current methods to characterize such changes either provide very limited information on many cells or have extremely low throughput. We introduce electro-acoustic spinning (EAS). Cells were found to spin in combined non-rotating AC electric and acoustic fields. The rotation velocity in EAS depends critically on a cell's electrical and mechanical properties. In contrast to existing methods, the rotation is uniform in the field of view and hundreds of cells can be characterized simultaneously. We demonstrate that EAS can distinguish cells with only minor differences in electric and mechanical properties, including differences in age or the number of passages.
Topics: Electricity; Rotation; Acoustics; Acoustic Stimulation; Electric Stimulation; Cochlear Implants; Cochlear Implantation
PubMed: 37993513
DOI: 10.1038/s41598-023-46550-w -
Trends in Microbiology Nov 2021The bacterial flagellar motor, a remarkable rotary machine, can rapidly switch between counterclockwise (CCW) and clockwise (CW) rotational directions to control the... (Review)
Review
The bacterial flagellar motor, a remarkable rotary machine, can rapidly switch between counterclockwise (CCW) and clockwise (CW) rotational directions to control the migration behavior of the bacterial cell. The flagellar motor consists of a bidirectional spinning rotor surrounded by torque-generating stator units. Recent high-resolution in vitro and in situ structural studies have revealed stunning details of the individual components of the flagellar motor and their interactions in both the CCW and CW senses. In this review, we discuss these structures and their implications for understanding the molecular mechanisms underlying flagellar rotation and switching.
Topics: Bacteria; Bacterial Proteins; Flagella; Molecular Motor Proteins; Rotation; Torque
PubMed: 33865677
DOI: 10.1016/j.tim.2021.03.009 -
Sensors (Basel, Switzerland) Apr 2022Among various energy harvesting technologies, triboelectricity is an epoch-making discovery that can convert energy loss caused by the mechanical vibration or friction...
Among various energy harvesting technologies, triboelectricity is an epoch-making discovery that can convert energy loss caused by the mechanical vibration or friction of parts into energy gain. As human convenience has emerged as an important future value, wireless devices have attracted widespread attention; thus, it is essential to extend the duration and lifespan of batteries through energy harvesting or the application of self-powered equipment. Here, we report a transistor, in which the gate rotates and rubs against the dielectric and utilizes the triboelectricity generated rather than the switching voltage of the transistor. The device is a triboelectric transistor with a simple structure and is manufactured using a simple process. Compared to that at the stationary state, the output current of the triboelectric transistor increased by 207.66 times at the maximum rotation velocity. The approach reported in this paper could be an innovative method to enable a transistor to harness its own power while converting energy loss in any rotating object into harvested energy.
Topics: Electric Power Supplies; Humans; Nanotechnology; Rotation
PubMed: 35590998
DOI: 10.3390/s22093309 -
Nature Communications May 2022For many bacteria, motility stems from one or more flagella, each rotated by the bacterial flagellar motor, a powerful rotary molecular machine. The hook, a soft polymer...
For many bacteria, motility stems from one or more flagella, each rotated by the bacterial flagellar motor, a powerful rotary molecular machine. The hook, a soft polymer at the base of each flagellum, acts as a universal joint, coupling rotation between the rigid membrane-spanning rotor and rigid flagellum. In multi-flagellated species, where thrust arises from a hydrodynamically coordinated flagellar bundle, hook flexibility is crucial, as flagella rotate significantly off-axis. However, consequently, the thrust applies a significant bending moment. Therefore, the hook must simultaneously be compliant to enable bundle formation yet rigid to withstand large hydrodynamical forces. Here, via high-resolution measurements and analysis of hook fluctuations under dynamical conditions, we elucidate how it fulfills this double functionality: the hook shows a dynamic increase in bending stiffness under increasing torsional stress. Such strain-stiffening allows the system to be flexible when needed yet reduce deformation under high loads, enabling high speed motility.
Topics: Bacteria; Bacterial Proteins; Cell Membrane Structures; Flagella; Rotation
PubMed: 35614041
DOI: 10.1038/s41467-022-30295-7 -
BMJ Global Health Jul 2021Rotation work, characterised by travelling long distances to work in isolated areas where workers typically rotate consecutive days working and living on-site with...
INTRODUCTION
Rotation work, characterised by travelling long distances to work in isolated areas where workers typically rotate consecutive days working and living on-site with periods at home, is increasingly used in the resources and construction sectors globally. Such employment practices may have an impact on workers' health and well-being. This systematic review explores the impact rotation work has on mental and physical outcomes in rotation workers in the resources and construction sectors.
METHOD
The PubMed, Medline, EMBASE, CINAHL, PsycINFO, and Scopus databases were systematically searched on 1 May 2020 to identify quantitative, qualitative and mixed-method studies addressing the health of rotation workers published in peer-reviewed journals. Findings from the studies were summarised narratively.
RESULTS
Of 6268 studies retrieved, 90 studies were included in the review. Studies suggested higher prevalence of psychological distress in onshore rotation workers and higher overweight/obesity rates among rotation workers as compared with the general population. We found more sleep problems and higher levels of smoking during work periods compared with off-site days; and higher alcohol intake during off-site days compared with on-site days. Workers generally perceived their physical health status as good. High-perceived job demands (such as workload, repetitive work) were associated with mental distress and exhaustion, sleep problems and perceived poor physical health status, while high-perceived job resources (such as job clarity/control, support) were associated with low mental distress and exhaustion, less smoking and alcohol intake, and better sleep.
CONCLUSION
Rotation work is associated with several poorer health behaviours and outcomes, such as sleep problems, smoking, alcohol consumption and overweight/obesity. Interventions needed to improve rotation workers' health should include maximising available job resources and reducing job demands. Further longitudinal studies are needed to explore the long-term health effects of rotation work and the short-term contextual effects of the different aspects of rotation work.
Topics: Construction Industry; Humans; Occupational Health; Rotation; Workload
PubMed: 34301674
DOI: 10.1136/bmjgh-2021-005112 -
Sensors (Basel, Switzerland) Mar 2022The requirement to estimate the six degree-of-freedom pose of a moving platform frequently arises in automation applications. It is common to estimate platform pose by...
The requirement to estimate the six degree-of-freedom pose of a moving platform frequently arises in automation applications. It is common to estimate platform pose by the fusion of global navigation satellite systems (GNSS) measurements and translational acceleration and rotational rate measurements from an inertial measurement unit (IMU). This paper considers a specific situation where two GNSS receivers and one IMU are used and gives the full formulation of a Kalman filter-based estimator to do this. A limitation in using this sensor set is the difficulty of obtaining accurate estimates of the degree of freedom corresponding to rotation about the line passing through the two GNSS receiver antenna centres. The GNSS-aided IMU formulation is extended to incorporate LiDAR measurements in both known and unknown environments to stabilise this degree of freedom. The performance of the pose estimator is established by comparing expected LiDAR range measurements with actual range measurements. Distributions of the terrain point-to-model error are shown to improve from 0.27m mean error to 0.06m when the GNSS-aided IMU estimator is augmented with LiDAR measurements. This precision is marginally degraded to 0.14m when the pose estimator is operated in an unknown environment.
Topics: Acceleration; Rotation
PubMed: 35336417
DOI: 10.3390/s22062248 -
Cortex; a Journal Devoted To the Study... Sep 2023Motor learning depends on the joint contribution of several processes including cognitive strategies aiming at goal achievement and prediction error-driven implicit...
Motor learning depends on the joint contribution of several processes including cognitive strategies aiming at goal achievement and prediction error-driven implicit adaptation. Understanding this functional interplay and its clinical implications requires insight into the individual learning processes, including at a neural level. Here, we set out to examine the impact of learning a cognitive strategy, over and above implicit adaptation, on the oscillatory post-movement β rebound (PMBR), which typically decreases in power following (visuo)motor perturbations. Healthy participants performed reaching movements towards a target, with online visual feedback replacing the view of their moving hand. The feedback was sometimes rotated, either relative to their movements (visuomotor rotation) or invariant to their movements (and relative to the target; clamped feedback), always for two consecutive trials interspersed between non-rotated trials. In both conditions, the first trial with a rotation was unpredictable. On the second trial, the task was either to re-aim, and thereby compensate for the rotation experienced in the first trial (visuomotor rotation; Compensate condition), or to ignore the rotation and keep on aiming at the target (clamped feedback; Ignore condition). After-effects did not differ between conditions, indicating that the amount of implicit learning was similar, while large differences in movement direction in the second rotated trial between conditions indicated that participants successfully acquired re-aiming strategies. Importantly, PMBR power following the first rotated trial was modulated differently in the two conditions. Specifically, it decreased in both conditions, but this effect was larger when participants had to acquire a cognitive strategy and prepare to re-aim. Our results therefore suggest that the PMBR is modulated by cognitive demands of motor learning, possibly reflecting the evaluation of a behaviourally significant goal achievement error.
Topics: Humans; Psychomotor Performance; Learning; Movement; Hand; Adaptation, Physiological; Feedback, Sensory; Rotation; Visual Perception
PubMed: 37295237
DOI: 10.1016/j.cortex.2023.05.002 -
Biochimica Et Biophysica Acta Aug 2010Two proton pumps, the F-ATPase (ATP synthase, FoF1) and the V-ATPase (endomembrane proton pump), have different physiological functions, but are similar in subunit... (Review)
Review
Two proton pumps, the F-ATPase (ATP synthase, FoF1) and the V-ATPase (endomembrane proton pump), have different physiological functions, but are similar in subunit structure and mechanism. They are composed of a membrane extrinsic (F1 or V1) and a membrane intrinsic (Fo or Vo) sector, and couple catalysis of ATP synthesis or hydrolysis to proton transport by a rotational mechanism. The mechanism of rotation has been extensively studied by kinetic, thermodynamic and physiological approaches. Techniques for observing subunit rotation have been developed. Observations of micron-length actin filaments, or polystyrene or gold beads attached to rotor subunits have been highly informative of the rotational behavior of ATP hydrolysis-driven rotation. Single molecule FRET experiments between fluorescent probes attached to rotor and stator subunits have been used effectively in monitoring proton motive force-driven rotation in the ATP synthesis reaction. By using small gold beads with diameters of 40-60 nm, the E. coli F1 sector was found to rotate at surprisingly high speeds (>400 rps). This experimental system was used to assess the kinetics and thermodynamics of mutant enzymes. The results revealed that the enzymatic reaction steps and the timing of the domain interactions among the beta subunits, or between the beta and gamma subunits, are coordinated in a manner that lowers the activation energy for all steps and avoids deep energy wells through the rotationally-coupled steady-state reaction. In this review, we focus on the mechanism of steady-state F1-ATPase rotation, which maximizes the coupling efficiency between catalysis and rotation.
Topics: Adenosine Triphosphate; Animals; Catalysis; Humans; Proton-Translocating ATPases; Rotation; Thermodynamics; Vacuolar Proton-Translocating ATPases
PubMed: 20170625
DOI: 10.1016/j.bbabio.2010.02.014 -
Scientific Reports Sep 2021The retrosplenial complex (RSC) plays a crucial role in spatial orientation by computing heading direction and translating between distinct spatial reference frames...
The retrosplenial complex (RSC) plays a crucial role in spatial orientation by computing heading direction and translating between distinct spatial reference frames based on multi-sensory information. While invasive studies allow investigating heading computation in moving animals, established non-invasive analyses of human brain dynamics are restricted to stationary setups. To investigate the role of the RSC in heading computation of actively moving humans, we used a Mobile Brain/Body Imaging approach synchronizing electroencephalography with motion capture and virtual reality. Data from physically rotating participants were contrasted with rotations based only on visual flow. During physical rotation, varying rotation velocities were accompanied by pronounced wide frequency band synchronization in RSC, the parietal and occipital cortices. In contrast, the visual flow rotation condition was associated with pronounced alpha band desynchronization, replicating previous findings in desktop navigation studies, and notably absent during physical rotation. These results suggest an involvement of the human RSC in heading computation based on visual, vestibular, and proprioceptive input and implicate revisiting traditional findings of alpha desynchronization in areas of the navigation network during spatial orientation in movement-restricted participants.
Topics: Adult; Alpha Rhythm; Female; Gyrus Cinguli; Head Movements; Humans; Occipital Lobe; Orientation, Spatial; Parietal Lobe; Rotation
PubMed: 34521939
DOI: 10.1038/s41598-021-97749-8 -
Optics Express Oct 2008The optical cell rotator (OCR) is a modified dual-beam laser trap for the holding and controlled rotation of suspended dielectric microparticles, such as cells. In...
The optical cell rotator (OCR) is a modified dual-beam laser trap for the holding and controlled rotation of suspended dielectric microparticles, such as cells. In contrast to optical tweezers, OCR uses two counter-propagating divergent laser beams, which are shaped and delivered by optical fibers. The rotation of a trapped specimen is carried out by the rotation of a dual-mode fiber, emitting an asymmetric laser beam. Experiments were performed on human erythrocytes, promyelocytic leukemia cells (HL60), and cell clusters (MCF-7). Since OCR permits the rotation of cells around an axis perpendicular to the optical axis of any microscope and is fully decoupled from imaging optics, it could be a suitable and expedient tool for tomographic microscopy.
Topics: Cell Separation; Equipment Design; Equipment Failure Analysis; Micromanipulation; Optical Fibers; Optical Tweezers; Rotation
PubMed: 18852807
DOI: 10.1364/oe.16.016984