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Environmental Pollution (Barking, Essex... Oct 2022Many communities around the country are undergoing contentious battles over the installation of artificial turf. Opponents are concerned about exposure to hazardous... (Review)
Review
Many communities around the country are undergoing contentious battles over the installation of artificial turf. Opponents are concerned about exposure to hazardous chemicals leaching from the crumb rubber cushioning fill made of recycled tires, the plastic carpet, and other synthetic components. Numerous studies have shown that chemicals identified in artificial turf, including polycyclic aromatic hydrocarbons (PAHs), phthalates, and per- and polyfluoroalkyl substances (PFAS), are known carcinogens, neurotoxicants, mutagens, and endocrine disruptors. However, few studies have looked directly at health outcomes of exposure to these chemicals in the context of artificial turf. Ecotoxicology studies in invertebrates exposed to crumb rubber have identified risks to organisms whose habitats have been contaminated by artificial turf. Chicken eggs injected with crumb rubber leachate also showed impaired development and endocrine disruption. The only human epidemiology studies conducted related to artificial turf have been highly limited in design, focusing on cancer incidence. In addition, government agencies have begun their own risk assessment studies to aid community decisions. Additional studies in in vitro and in vivo translational models, ecotoxicological systems, and human epidemiology are strongly needed to consider exposure from both field use and runoff, components other than crumb rubber, sensitive windows of development, and additional physiological endpoints. Identification of potential health effects from exposures due to spending time at artificial turf fields and adjacent environments that may be contaminated by runoff will aid in risk assessment and community decision making on the use of artificial turf.
Topics: Environmental Exposure; Hazardous Substances; Humans; Polycyclic Aromatic Hydrocarbons; Recycling; Rubber
PubMed: 35948114
DOI: 10.1016/j.envpol.2022.119841 -
Applied Microbiology and Biotechnology Jan 2019Natural rubber (NR), poly(cis-1,4-isoprene), is used in an industrial scale for more than 100 years. Most of the NR-derived materials are released to the environment as... (Review)
Review
Natural rubber (NR), poly(cis-1,4-isoprene), is used in an industrial scale for more than 100 years. Most of the NR-derived materials are released to the environment as waste or by abrasion of small particles from our tires. Furthermore, compounds with isoprene units in their molecular structures are part of many biomolecules such as terpenoids and carotenoids. Therefore, it is not surprising that NR-degrading bacteria are widespread in nature. NR has one carbon-carbon double bond per isoprene unit and this functional group is the primary target of NR-cleaving enzymes, so-called rubber oxygenases. Rubber oxygenases are secreted by rubber-degrading bacteria to initiate the break-down of the polymer and to use the generated cleavage products as a carbon source. Three main types of rubber oxygenases have been described so far. One is rubber oxygenase RoxA that was first isolated from Xanthomonas sp. 35Y but was later also identified in other Gram-negative rubber-degrading species. The second type of rubber oxygenase is the latex clearing protein (Lcp) that has been regularly found in Gram-positive rubber degraders. Recently, a third type of rubber oxygenase (RoxB) with distant relationship to RoxAs was identified in Gram-negative bacteria. All rubber oxygenases described so far are haem-containing enzymes and oxidatively cleave polyisoprene to low molecular weight oligoisoprenoids with terminal CHO and CO-CH functions between a variable number of intact isoprene units, depending on the type of rubber oxygenase. This contribution summarises the properties of RoxAs, RoxBs and Lcps.
Topics: Bacteria; Bacterial Proteins; Biotechnology; Electron Spin Resonance Spectroscopy; Heme; Hemiterpenes; Latex; Oxygenases; Phylogeny; Rubber; Spectrophotometry, Ultraviolet; Xanthomonas
PubMed: 30377752
DOI: 10.1007/s00253-018-9453-z -
International Journal of Molecular... Dec 2018Natural rubber is a kind of indispensable biopolymers with great use and strategic importance in human society. However, its production relies almost exclusively on... (Review)
Review
Natural rubber is a kind of indispensable biopolymers with great use and strategic importance in human society. However, its production relies almost exclusively on rubber-producing plants , which have high requirements for growth conditions, and the mechanism of natural rubber biosynthesis remains largely unknown. In the past two decades, details of the rubber chain polymerization and proteins involved in natural rubber biosynthesis have been investigated intensively. Meanwhile, omics and other advanced biotechnologies bring new insight into rubber production and development of new rubber-producing plants. This review summarizes the achievements of the past two decades in understanding the biosynthesis of natural rubber, especially the massive information obtained from the omics analyses. Possibilities of natural rubber biosynthesis in vitro or in genetically engineered microorganisms are also discussed.
Topics: Biotechnology; Genetic Engineering; Genome, Plant; Hevea; Humans; In Vitro Techniques; Microorganisms, Genetically-Modified; Plant Proteins; Proteome; Rubber; Transcriptome
PubMed: 30583567
DOI: 10.3390/ijms20010050 -
International Journal of Environmental... Nov 2022Antioxidants are prevalently used during rubber production to improve rubber performance, delay aging, and extend service life. However, recent studies have revealed... (Review)
Review
Antioxidants are prevalently used during rubber production to improve rubber performance, delay aging, and extend service life. However, recent studies have revealed that their transformation products (TPs) could adversely affect environmental organisms and even lead to environmental events, which led to great public concern about environmental occurrence and potential impacts of rubber antioxidants and their TPs. In this review, we first summarize the category and application of rubber antioxidants in the world, and then demonstrate the formation mechanism of their TPs in the environment, emphasizing their influence on the ozone oxidative degradation. The potential toxic effects of antioxidants and their TPs are further reviewed to improve understanding of their biological health impact and environmental risks. Finally, the environmental occurrences of antioxidants and their TPs are summarized and their environmental impacts are demonstrated based on the recent studies. Due to the currently limited understanding on the toxic and biological effects of these compounds, further studies are required in order to better assess various TPs of these antioxidants and their environmental impact. To our knowledge, this is the first review on antioxidants and their TPs in the environment, which may elevate the environmental risk awareness of rubber products and their TPs in the near future.
Topics: Water Pollutants, Chemical; Rubber; Antioxidants
PubMed: 36361475
DOI: 10.3390/ijerph192114595 -
Plant Biotechnology Journal Nov 2019Natural rubber (NR) is a nonfungible and valuable biopolymer, used to manufacture ~50 000 rubber products, including tires and medical gloves. Current production of NR... (Review)
Review
Natural rubber (NR) is a nonfungible and valuable biopolymer, used to manufacture ~50 000 rubber products, including tires and medical gloves. Current production of NR is derived entirely from the para rubber tree (Hevea brasiliensis). The increasing demand for NR, coupled with limitations and vulnerability of H. brasiliensis production systems, has induced increasing interest among scientists and companies in potential alternative NR crops. Genetic/metabolic pathway engineering approaches, to generate NR-enriched genotypes of alternative NR plants, are of great importance. However, although our knowledge of rubber biochemistry has significantly advanced, our current understanding of NR biosynthesis, the biosynthetic machinery and the molecular mechanisms involved remains incomplete. Two spatially separated metabolic pathways provide precursors for NR biosynthesis in plants and their genes and enzymes/complexes are quite well understood. In contrast, understanding of the proteins and genes involved in the final step(s)-the synthesis of the high molecular weight rubber polymer itself-is only now beginning to emerge. In this review, we provide a critical evaluation of recent research developments in NR biosynthesis, in vitro reconstitution, and the genetic and metabolic pathway engineering advances intended to improve NR content in plants, including H. brasiliensis, two other prospective alternative rubber crops, namely the rubber dandelion and guayule, and model species, such as lettuce. We describe a new model of the rubber transferase complex, which integrates these developments. In addition, we highlight the current challenges in NR biosynthesis research and future perspectives on metabolic pathway engineering of NR to speed alternative rubber crop commercial development.
Topics: Hevea; Metabolic Engineering; Rubber; Transferases
PubMed: 31150158
DOI: 10.1111/pbi.13181 -
Waste Management (New York, N.Y.) Aug 2022Waste tires management is a serious and global environmental problem. Therefore, searching for low-cost and industrial-scale applicable tire recycling methods is gaining... (Review)
Review
Waste tires management is a serious and global environmental problem. Therefore, searching for low-cost and industrial-scale applicable tire recycling methods is gaining more and more attention. Waste tire rubber is valuable source of secondary raw materialsforthecircular economy and current trends indicate that application of waste rubbers during manufacturing value-added productsshould increase in near future. Sustainable development of rubber devulcanization technologies and appropriate design of cradle-to-cradle loops for rubber goods are the most promising strategies for achieving a higher level of rubber recycling. This work presents the state-of-the-art in the patented waste tire rubber devulcanization technologies including dynamic desulfurization, reactive extrusion, microwave treatment, and also other less popular methods. Special attention was focused on the used components, rubber treatment conditions and static mechanical properties of reclaimed rubbers. Moreover, environmental aspects and limitations related to rubber devulcanization technologies implementation are also discussed. Our findings showed that reclaimed rubbers described in patents are characterized by higher tensile strength and elongation break (depending on devulcanization technology median: 16.6-19.0 MPa and 321-443%, respectively) compared to the literature data (median: 10.3 MPa and 309%) or commercial products (median: 6.8 MPa and 250%). The significant differences observed in performance properties of reclaimed rubbers resulted mainly from devulcanization efficiency related to waste tires composition or source and rubber treatment conditions. Considering environmental and economic aspects, reactive extrusion is the most promising method further development rubber devulcanization technologies.
Topics: Microwaves; Recycling; Rubber; Technology; Tensile Strength
PubMed: 35843055
DOI: 10.1016/j.wasman.2022.07.002 -
International Journal of Molecular... Aug 2022IAN873, Dongfang93114 and Reyan73397, created through vegetative propagation for their high yield and excellent cold resistance, are major clones planted in China. In...
IAN873, Dongfang93114 and Reyan73397, created through vegetative propagation for their high yield and excellent cold resistance, are major clones planted in China. In this work, latexes with rubber particles of the same size from these clones are separated from fresh natural rubber latex, and corresponding rubber films are prepared from each latex. The structure and components of each film are measured. This indicates that the characteristics of the rubbers obtained from latexes with similar particle sizes show some resembling trends among different clones, while for specific samples, those characteristics vary depending on the clone. The molecular weight is generally highest in IAN873 and lowest in Reyan73397. Rubber chains in small rubber particles are longer, and large rubber particles show a wider molecular weight distribution. The gel content of every sample from Reyan73397 is lower than the other two clones. The nitrogen content increases with the size of rubber particles in all clones. The ester content of small rubber particles in IAN873 and Reyan73397 is almost zero. Large rubber particles have more branching points formed via esters. This study provides a new perspective on the influence of clones on the relationship between characteristics of natural rubber and the size of rubber particles in natural rubber latex.
Topics: Clone Cells; Latex; Molecular Weight; Plant Proteins; Plants; Rubber
PubMed: 36012145
DOI: 10.3390/ijms23168880 -
PeerJ 2022Rainforest canopies, home to one of the most complex and diverse terrestrial arthropod communities, are threatened by conversion of rainforest into agricultural...
Rainforest canopies, home to one of the most complex and diverse terrestrial arthropod communities, are threatened by conversion of rainforest into agricultural production systems. However, little is known about how predatory arthropod communities respond to such conversion. To address this, we compared canopy spider (Araneae) communities from lowland rainforest with those from three agricultural systems in Jambi Province, Sumatra, Indonesia, i.e., jungle rubber (rubber agroforest) and monoculture plantations of rubber and oil palm. Using canopy fogging, we collected 10,676 spider specimens belonging to 36 families and 445 morphospecies. The four most abundant families (Salticidae = 2,043, Oonopidae = 1,878, Theridiidae = 1,533 and Clubionidae = 1,188) together comprised 62.2% of total individuals, while the four most speciose families, Salticidae (S = 87), Theridiidae (S = 83), Araneidae (S = 48) and Thomisidae (S = 39), contained 57.8% of all morphospecies identified. In lowland rainforest, average abundance, biomass and species richness of canopy spiders was at least twice as high as in rubber or oil palm plantations, with jungle rubber showing similar abundances as rainforest, and intermediate biomass and richness. Community composition of spiders was similar in rainforest and jungle rubber, but differed from rubber and oil palm, which also differed from each other. Canonical Correspondence Analysis showed that canopy openness, aboveground tree biomass and tree density together explained 18.2% of the variation in spider communities at family level. On a morphospecies level, vascular plant species richness and tree density significantly affected the community composition but explained only 6.8% of the variance. While abundance, biomass and diversity of spiders declined strongly with the conversion of rainforest into monoculture plantations of rubber and oil palm, we also found that a large proportion of the rainforest spider community can thrive in extensive agroforestry systems such as jungle rubber. Despite being very different from rainforest, the canopy spider communities in rubber and oil palm plantations may still play a vital role in the biological control of canopy herbivore species, thus contributing important ecosystem services. The components of tree and palm canopy structure identified as major determinants of canopy spider communities may aid in decision-making processes toward establishing cash-crop plantation management systems which foster herbivore control by spiders.
Topics: Animals; Biomass; Ecosystem; Rainforest; Rubber; Spiders; Arthropods; Trees
PubMed: 35990898
DOI: 10.7717/peerj.13898 -
Water Research Feb 2020Synthetic polymer-based materials are ubiquitous in aquatic environments, where weathering processes lead to their progressive fragmentation and the leaching of additive...
Synthetic polymer-based materials are ubiquitous in aquatic environments, where weathering processes lead to their progressive fragmentation and the leaching of additive chemicals. The current study assessed the chemical content of freshwater and marine leachates produced from car tire rubber (CTR), polypropylene (PP), polyethylene terephthalate (PET), polystyrene (PS) and polyvinyl chloride (PVC) microplastics, and their adverse effects on the microalgae Raphidocelis subcapitata (freshwater) and Skeletonema costatum (marine) and the Mediterranean mussel Mytilus galloprovincialis. A combination of non-target and target chemical analysis revealed a number of organic and metal compounds in the leachates, including representing plasticizers, antioxidants, antimicrobials, lubricants, and vulcanizers. CTR and PVC materials and their corresponding leachates had the highest content of tentatively identified organic additives, while PET had the lowest. The metal content varied both between polymer leachates and between freshwater and seawater. Notable additives identified in high concentrations were benzothiazole (CTR), phthalide (PVC), acetophenone (PP), cobalt (CTR, PET), zinc (CTR, PVC), lead (PP) and antimony (PET). All leachates, except PET, inhibited algal growth with EC values ranging from 0.5% (CTR) and 64% (PP) of the total leachate concentration. Leachates also affected mussel endpoints, including the lysosomal membrane stability and early stages endpoints as gamete fertilization, embryonic development and larvae motility and survival. Embryonic development was the most sensitive parameter in mussels, with EC values ranging from 0.8% (CTR) to 65% (PET) of the total leachate. The lowest impacts were induced on D-shell larvae survival, reflecting their ability to down-regulate motility and filtration in the presence of chemical stressors. This study provides evidence of the relationship between chemical composition and toxicity of plastic/rubber leachates. Consistent with increasing contamination by organic and inorganic additives, the leachates ranged from slightly to highly toxic to mussels and algae, highlighting the need for a better understanding of the overall impact of plastic-associated chemicals on aquatic ecosystems.
Topics: Animals; Aquatic Organisms; Automobiles; Ecosystem; Plastics; Rubber; Water Pollutants, Chemical
PubMed: 31731243
DOI: 10.1016/j.watres.2019.115270 -
Dento Maxillo Facial Radiology Jan 2022The aim of this study was to construct an anthropomorphic maxillofacial phantom for dental imaging and dosimetry purposes using three-dimensional (3D) printing...
OBJECTIVE
The aim of this study was to construct an anthropomorphic maxillofacial phantom for dental imaging and dosimetry purposes using three-dimensional (3D) printing technology and materials that simulate the radiographic properties of tissues.
METHODS
Stereolithography photoreactive resins, polyurethane rubber and epoxy resin were modified by adding calcium carbonate and strontium carbonate powders or glass bubbles. These additives were used to change the materials' CT numbers to mimic various body tissues. A maxillofacial phantom was designed using CT images of a head.
RESULTS
Commercial 3D printing resins were found to have CT numbers near 120 HU and were used to print intervertebral discs and an external skin for the maxillofacial phantom. By adding various amounts of calcium carbonate and strontium carbonate powders the CT number of the resin was raised to 1000 & 1500 HU and used to print bone mimics. Epoxy resin modified by adding glass bubbles was used in assembly and as a cartilaginous mimic. Glass bubbles were added to polyurethane rubber to reduce the CT number to simulate soft tissue and filled spaces between the printed anatomy and external skin of the phantom.
CONCLUSION
The maxillofacial phantom designed for dental imaging and dosimetry constructed using 3D printing, polyurethane rubbers and epoxy resins represented a patient anatomically and radiographically. The results of the designed phantom, materials and assembly process can be applied to generate different phantoms that better represent diverse patient types and accommodate different ion chambers.
Topics: Epoxy Resins; Humans; Phantoms, Imaging; Polyurethanes; Printing, Three-Dimensional; Rubber; Tomography, X-Ray Computed
PubMed: 34133225
DOI: 10.1259/dmfr.20200323