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Ecotoxicology and Environmental Safety Mar 2021Emerging research in mammalian cells suggests that ionic (AgNO) and nano silver (AgNP) can disrupt the metabolism of selenium which plays a vital role in oxidative...
Emerging research in mammalian cells suggests that ionic (AgNO) and nano silver (AgNP) can disrupt the metabolism of selenium which plays a vital role in oxidative stress control. However, the effect of silver (Ag) on selenoprotein function in fish is poorly understood. Here we evaluate the effects of AgNO and citrate coated AgNP (cit-AgNP) on selenoprotein function and oxidative stress using a fish cell line derived from the rainbow trout (Oncorhynchus mykiss) intestine (RTgutGC). Cell viability was evaluated using a cytotoxicity assay which measures simultaneously metabolic activity, membrane integrity and lysosome integrity. Cells exposed to equimolar amounts of AgNO and cit-AgNP accumulated the same amount of silver intracellularly, however AgNO was more toxic than cit-AgNP. Selenoenzymes glutathione peroxidase (GPx) and thioredoxin reductase (TrxR) mRNA levels and enzyme activity were measured. While mRNA levels remained unaffected by AgNO or cit-AgNP, the enzyme activity of GPx was inhibited by AgNO (1 µM) and cit-AgNP (5 µM) and TrxR activity was inhibited by AgNO (0.4 µM) and cit-AgNP (1, 5 µM). Moreover, cells exposed to 1 µM of AgNO and cit-AgNP showed an increase in metallothionein b (MTb) mRNA levels at 24 h of exposure, confirming the uptake of silver, but returned to control levels at 72 h suggesting silver scavenging by MTb. Oxidative stress was not observed at any of the doses of AgNO or cit-AgNP tested. Overall, this study shows that AgNO or cit-AgNP can inhibit the activity of selenoenzymes but do not induce oxidative stress in RTgutGC cells.
Topics: Animals; Cell Line; Cell Survival; Citrates; Citric Acid; Glutathione Peroxidase; Intestines; Ions; Metal Nanoparticles; Metallothionein; Oncorhynchus mykiss; Oxidative Stress; Selenium; Selenoproteins; Silver; Silver Nitrate
PubMed: 33472113
DOI: 10.1016/j.ecoenv.2021.111930 -
Science Progress 2023The tobacco cutworm () is a widespread pest that inflicts severe damage on various crops, including cotton, tobacco, and vegetables, with a particular preference for...
The tobacco cutworm () is a widespread pest that inflicts severe damage on various crops, including cotton, tobacco, and vegetables, with a particular preference for solanaceous plants. Traditional control methods often rely heavily on synthetic insecticides, leading to adverse effects on the environment, human health, and the development of insecticide resistance. In light of these challenges, this study explores the potential of nanotechnology as an innovative and sustainable approach to combat this notorious pest. Bioassays were conducted using laboratory-reared 3rd instar larvae. Eight different plant extracts coated with zinc oxide and silver nitrate nanoparticles were tested, with concentrations in both distilled water and ethanol at 3, 5, and 7 ml. Data were collected at 24, 48, and 72-h intervals. The results revealed that the highest larval mortality, reaching 98%, was observed in the group treated with silver nitrate nanoparticles derived from . In comparison, the group treated with zinc oxide nanoparticles dissolved in ethanol exhibited a larval mortality rate of 90%. Ethanol is a polar solvent that is widely used in the synthesis of nanocomposites. It is capable of forming strong hydrogen bonds with oxygen atoms, making it a good dispersant for zinc oxide nanoparticles. Additionally, ethanol has a low boiling point and a non-toxic nature, which makes it a safe and effective option for the dispersion of nanoparticles. Notably, the study concluded that silver nanoparticles combined with ethanol exhibited prolonged and more potent toxic effects against when compared to zinc oxide nanoparticles. Overall, this research underscores the potential of nanotechnology as a valuable component of Integrated Pest Management (IPM) strategies. By integrating nanotechnology into pest management practices, we can promote sustainable and environmentally friendly approaches that benefit both farmers and the ecosystem.
Topics: Animals; Ecosystem; Ethanol; Larva; Metal Nanoparticles; Nitrates; Plant Extracts; Silver; Silver Nitrate; Spodoptera; Zinc Oxide; Pest Control
PubMed: 38113117
DOI: 10.1177/00368504231219171 -
Scientific Reports May 2022Rubia cordifolia L. is a widely used traditional medicine in the Indian sub-continent and Eastern Asia. In the present study, the aqueous leaf extract of the R....
Rubia cordifolia L. is a widely used traditional medicine in the Indian sub-continent and Eastern Asia. In the present study, the aqueous leaf extract of the R. Cordifolia was used to fabricate silver nanoparticles (RC@AgNPs), following a green synthesis approach. Effect of temperature (60 °C), pH (8), as well the concentration of leaf extract (2 ml) and silver nitrate (2 mM) were optimized for the synthesis of stable RC@AgNPs. The phytofabrication of nanosilver was validated by UV-visible spectral analysis, which displayed a distinctive surface plasmon resonance peak at 432 nm. The effective functional molecules as capping and stabilizing agents, and responsible for the conversion of Ag to nanosilver (Ag) were identified using the FTIR spectra. The spherical RC@AgNPs with an average size of ~ 20.98 nm, crystalline nature, and 61% elemental composition were revealed by TEM, SEM, XRD, and. EDX. Biogenic RC@AgNPs displayed a remarkable anticancer activity against B16F10 (melanoma) and A431 (carcinoma) cell lines with respective IC of 36.63 and 54.09 µg/mL, respectively. Besides, RC@AgNPs showed strong antifungal activity against aflatoxigenic Aspergillus flavus, DNA-binding properties, and DPPH and ABTS free radical inhibition. The presented research provides a potential therapeutic agent to be utilized in various biomedical applications.
Topics: Metal Nanoparticles; Plant Extracts; Rubia; Silver; Silver Nitrate
PubMed: 35614187
DOI: 10.1038/s41598-022-12878-y -
Molecules (Basel, Switzerland) Sep 2022Silver nanoparticles (AgNPs) have recently gained interest in the medical field because of their biological features. The present study aimed at screening secondary...
Silver nanoparticles (AgNPs) have recently gained interest in the medical field because of their biological features. The present study aimed at screening secondary metabolites, quantifying their flavonoids and total phenolics content, green synthesis and characterization of silver nanoparticles. In addition, an assessment of in vitro cytotoxic, antioxidant, anti-inflammatory and wound healing activity of and its synthesized AgNPs was carried out. The powdered plant material (leaves) was subjected to Soxhlet extraction to obtain aqueous extract. The extract was used as a reducing agent in synthesizing AgNPs from silver nitrate. The synthesized AgNPs were characterized by UV-Vis, SEM-EDX, XRD, FTIR, particle size analyzer and zeta potential. Further aqueous leaf extract of and AgNPs was subjected for in vitro antioxidant, anti-inflammatory, wound healing and cytotoxic activity against A375 (Skin cancer), A549 (Lung cancer), and KB-3-1 (Oral cancer) cell lines. All experiments were repeated three times ( = 3), and the results were given as the mean ± SEM. The flavonoids and total phenolics content in extract were 44.18 ± 0.086 mg/g of quercetin and 53.24 ± 0.028 mg/g of gallic acid, respectively. SEM analysis revealed AgNPs with diameters ranging from 35 to 100 nm. XRD confirmed that the synthesized silver nanoparticles were crystalline in nature. The cytotoxicity cell viability assay revealed that the AgNPs were less toxic (IC 105.5 µg/mL) compared to the extract (IC 47.47 µg/mL) against the non-cancerous fibroblast L929 cell line. Antioxidant, anti-inflammatory, and cytotoxicity tests revealed that AgNPs had significantly more activity than the plant extract. The AgNPs inhibited protein denaturation by a mean percentage of 71.65%, which was equivalent to the standard anti-inflammatory medication diclofenac (94.24%). The AgNPs showed considerable cytotoxic effect, and the percentage of cell viability against skin cancer, lung cancer, and oral cancer cell lines was 31.84%, 56.09% and 22.59%, respectively. AgNPs demonstrated stronger cell migration and percentage of wound closure (82.79%) compared to the plant extract (75.23%). The overall results revealed that AgNPs exhibited potential antioxidant, anti-inflammatory, wound healing, and cytotoxic properties. In future, should be further explored to unmask its therapeutic potential and the mechanistic pathways of AgNPs should be studied in detail in in vivo animal models.
Topics: Animals; Anti-Bacterial Agents; Anti-Inflammatory Agents; Antineoplastic Agents; Antioxidants; Diclofenac; Gallic Acid; Metal Nanoparticles; Mouth Neoplasms; Plant Extracts; Quercetin; Reducing Agents; Rhizophoraceae; Silver; Silver Nitrate; Wound Healing
PubMed: 36234841
DOI: 10.3390/molecules27196306 -
Molecules (Basel, Switzerland) May 2021The main core of wound treatment is cell growth and anti-infection. To accelerate the proliferation of fibroblasts in the wound and prevent wound infections, various...
The main core of wound treatment is cell growth and anti-infection. To accelerate the proliferation of fibroblasts in the wound and prevent wound infections, various strategies have been tried. It remains a challenge to obtain good cell proliferation and antibacterial effects. Here, human hair kerateine (HHK)/poly(ethylene oxide) (PEO)/poly(vinyl alcohol) (PVA) nanofibers were prepared using cysteine-rich HHK, and then, silver nanoparticles (AgNPs) were in situ anchored in the sulfur-containing amino acid residues of HHK. After the ultrasonic degradation test, HHK/PEO/PVA nanofibrous mats treated with 0.005-M silver nitrate were selected due to their relatively complete structures. It was observed by TEM-EDS that the sulfur-containing amino acids in HHK were the main anchor points of AgNPs. The results of FTIR, XRD and the thermal analysis suggested that the hydrogen bonds between PEO and PVA were broken by HHK and, further, by AgNPs. AgNPs could act as a catalyst to promote the thermal degradation reaction of PVA, PEO and HHK, which was beneficial for silver recycling and medical waste treatment. The antibacterial properties of AgNP-HHK/PEO/PVA nanofibers were examined by the disk diffusion method, and it was observed that they had potential antibacterial capability against Gram-positive bacteria, Gram-negative bacteria and fungi. In addition, HHK in the nanofibrous mats significantly improved the cell proliferation of NIH3T3 cells. These results illustrated that the AgNP-HHK/PEO/PVA nanofibrous mats exhibited excellent antibacterial activity and the ability to promote the proliferation of fibroblasts, reaching our target applications.
Topics: Animals; Anti-Bacterial Agents; Candida albicans; Cell Proliferation; Escherichia coli; Hair; Humans; Hydrogen Bonding; Keratins, Hair-Specific; Metal Nanoparticles; Mice; Microbial Sensitivity Tests; NIH 3T3 Cells; Nanofibers; Polyethylene Glycols; Polyvinyl Alcohol; Silver; Silver Nitrate; Staphylococcus aureus; Wound Healing
PubMed: 34066875
DOI: 10.3390/molecules26092783 -
IET Nanobiotechnology Jul 2021Silver nanomaterials have become important research topics in recent years. As a new type of fluorescent material, silver nanomaterials have been applied to fluorescent...
Silver nanomaterials have become important research topics in recent years. As a new type of fluorescent material, silver nanomaterials have been applied to fluorescent sensors, bioimaging and materials targeting cancer cells. Here, an approach to the oligonucleotide-templated controllable formation of fluorescent Ag nanomaterials is reported. In this experiment, silver nanoparticles (NPs) were synthesised from oligonucleotides chains, sodium borohydride (NaBH ) and silver nitrate (AgNO ) by changing the molar ratio of DNA to sodium borohydride (NaBH ) and silver nitrate (AgNO ). Fluorescent assay and transmission electron microscopy were used to characterise the silver NPs. The optimal selection of DNA chains with different lengths as templates for the synthesis of silver NPs was found. This work successfully develops the capping oligonucleotides scaffolds of silver nanoclusters.
Topics: Metal Nanoparticles; Microscopy, Electron, Transmission; Oligonucleotides; Silver; Silver Nitrate
PubMed: 34694757
DOI: 10.1049/nbt2.12049 -
Comparative Biochemistry and... Jul 2022The massive production and use of silver nanoparticles (Ag NPs) have led to their increasing release into the environment. Even though the antimicrobial and cytotoxic...
The massive production and use of silver nanoparticles (Ag NPs) have led to their increasing release into the environment. Even though the antimicrobial and cytotoxic effects of native nanoparticles have been well studied, the environmental impacts of transformation products such as silver sulfide nanoparticles (AgS NPs) have not been elucidated. In the present study, we assessed the toxicity of AgS NPs and silver nitrate (AgNO), as a source of Ag, to the earthworm Eisenia andrei using a nominal concentration of 5 mg Ag kg soil. We used the OECD guidelines to assess effects on weight loss and mortality for 14 days. After exposure, we also extracted the immune effector cells (coelomocytes) and conducted a battery of biomarker tests. To ensure the quality of the toxicological results, the structural changes of NPs during the experiment and the uptake of silver by the earthworms were monitored. During the experiment, mortality effects were not detected, but a weight loss was observed in the earthworms exposed to AgS NPs. Altough AgS NPs were engulfed by E. andrei cells, neither phenoloxidase activity nor lipid peroxidation differed from the untreated control group. Cells from earthworms treated with AgS NPs exerted very broad value range of nitric oxide (NO) generation, suggesting an imbalance in the NO metabolism. Overall, this study suggests minimal risks associated with AgS NPs exposure to earthworms. However, further studies are needed to assure no immunotoxicological or chronic effects on a wider range of terrestrial organisms.
Topics: Animals; Metal Nanoparticles; Oligochaeta; Silver; Silver Compounds; Silver Nitrate; Soil; Soil Pollutants; Weight Loss
PubMed: 35489639
DOI: 10.1016/j.cbpc.2022.109355 -
Biomedica : Revista Del Instituto... Jun 2021Exogenous pigmentation by silver nitrate is a rare disease whose clinical manifestations appear even years after the contact, making its diagnosis difficult on...
Exogenous pigmentation by silver nitrate is a rare disease whose clinical manifestations appear even years after the contact, making its diagnosis difficult on occasions. It is characterized by the presence of blue-gray macules or plaques on the skin or mucosa in the contact area, sometimes very similar to melanocytic lesions and melanoma, which constitute the main differential diagnosis. We report the case of a male patient from Medellín, Colombia, with a family history of melanoma and the presence of these lesions throughout his body.
Topics: Argyria; Humans; Male; Melanoma; Pigmentation; Silver Nitrate; Skin
PubMed: 34214264
DOI: 10.7705/biomedica.5876 -
Molecules (Basel, Switzerland) Sep 2022In the current decade, nanoparticles are synthesized using solvents that are environmentally friendly. A number of nanoparticles have been synthesized at room...
An Evaluation of Antimicrobial, Anticancer, Anti-Inflammatory and Antioxidant Activities of Silver Nanoparticles Synthesized from Leaf Extract of Utilizing Quantitative and Qualitative Methods.
In the current decade, nanoparticles are synthesized using solvents that are environmentally friendly. A number of nanoparticles have been synthesized at room temperature using water as a solvent, such as gold (Au) and silver (Ag) nanoparticles. As part of nanotechnology, nanoparticles are synthesized through biological processes. Biological methods are the preferred method for the synthesis of inorganic nanoparticles (AgNPs) as a result of their simple and non-hazardous nature. Nanoparticles of silver are used in a variety of applications, including catalysts, spectrally selective coatings for solar absorption, optical objectives, pharmaceutical constituents, and chemical and biological sensing. Antimicrobial agents are among the top uses of silver nanoparticles. In the current study, silver nanoparticles were biologically manufactured through Madhuca longifolia, and their antibacterial activity against pathogenic microorganisms, anticancer, anti-inflammatory, and antioxidant activities were assessed. UV-Vis spectroscopy, XRD (X-ray diffraction), transmission electron microscopy, Zeta Potential, and FTIR were used to characterize silver nanoparticles. The current work describes a cheap and environmentally friendly method to synthesize silver nanoparticles from silver nitrate solution by using plant crude extract as a reducing agent.
Topics: Anti-Bacterial Agents; Anti-Infective Agents; Anti-Inflammatory Agents; Antioxidants; Gold; Madhuca; Metal Nanoparticles; Plant Extracts; Reducing Agents; Silver; Silver Nitrate; Solvents; Spectroscopy, Fourier Transform Infrared; Water; X-Ray Diffraction
PubMed: 36234942
DOI: 10.3390/molecules27196404 -
International Journal of Molecular... Jan 2024Recently, the utilization of biological agents in the green synthesis of nanoparticles has been given interest. In this study, silver nanoparticles were synthesized from...
Recently, the utilization of biological agents in the green synthesis of nanoparticles has been given interest. In this study, silver nanoparticles were synthesized from an aqueous extract of macrofungus (mushroom), namely , in a dark room using 20 µL of silver nitrate. Biosynthesized silver nanoparticles were confirmed by analyzing them using a UV-Vis (ultraviolet-visible) spectrophotometer. The synthesized silver nanoparticles were optimized at different pH and temperatures with various dosages of AgNO (silver nitrate) and fungal extracts. The synthesized AgNPs (silver nanoparticles) were characterized using TEM (transmission electron microscopy) and EDX (energy-dispersive X-ray) analyses, which confirmed the presence of silver nanoparticles. The size of the nanosilver particles was found to be 50 nm with higher stability. The mycosynthesized AgNPs showed effective antibacterial activity against strains of Gram-positive ( and ) and Gram-negative ( and ) bacteria. The minimum inhibitory concentration (MIC) was found to be 3.125 μg/mL by MIC assay. The MTT assay (3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl-2H-tetrazolium bromide) was performed to study cytotoxicity, and reduced cell viability was recorded at 100 μg/mL. Silver-Polygalacturonic acid-Polyvinyl alcohol ((Ag-PGA)-PVA) nanofiber was prepared using the electrospinning method. The in vitro wound scratch assay was demonstrated to study the wound-healing efficacy of the prepared nanofiber. The wound-healing efficacy of the AgNP-incorporated nanofiber was found to be 20% after 24 h. This study will lay a platform to establish a unique route to the development of a novel nanobiomaterial and its application in antibacterial and wound-healing therapy.
Topics: Escherichia coli; Metal Nanoparticles; Silver; Silver Nitrate; Anti-Bacterial Agents; Coloring Agents
PubMed: 38255936
DOI: 10.3390/ijms25020861