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Immunology Apr 2017The ocular surface is constantly exposed to environmental irritants, allergens and pathogens, against which it can mount a prompt immune response to preserve its... (Review)
Review
The ocular surface is constantly exposed to environmental irritants, allergens and pathogens, against which it can mount a prompt immune response to preserve its integrity. But to avoid unnecessary inflammation, the ocular surface's mucosal immune system must also discriminate between harmless and potentially dangerous antigens, a seemingly complicated task. Despite its unique features, the ocular surface is a mucosal lining, and as such, it shares some homeostatic and pathophysiological mechanisms with other mucosal surfaces. The purpose of this review is to explore the mucosal homeostatic immune function of the ocular surface in both the healthy and diseased states, with a special focus on mucosal immunology concepts. The information discussed in this review has been retrieved by PubMed searches for literature published from January 1981 to October 2016.
Topics: Allergens; Animals; Eye; Eye Diseases; Humans; Immune Tolerance; Immunity, Mucosal; Inflammation; Irritants
PubMed: 28108991
DOI: 10.1111/imm.12716 -
Optics Express Jul 2020Speckle is the spatial fluctuation of irradiance seen when coherent light is reflected from a rough surface. It is due to light reflected from the surface's many nooks...
Speckle is the spatial fluctuation of irradiance seen when coherent light is reflected from a rough surface. It is due to light reflected from the surface's many nooks and crannies accumulating vastly discrepant time delays, spanning much more than an optical period, en route to an observation point. Although speckle with continuous-wave (cw) illumination is well understood, the emerging interest in non-line-of-sight (NLoS) imaging using coherent light has created the need to understand the higher-order speckle that results from multiple rough-surface reflections, viz., speckled speckle and speckled speckled speckle. Moreover, the recent introduction of phasor-field (-field) NLoS imaging-which relies on amplitude-modulated coherent illumination-requires pushing beyond cw scenarios for speckle and higher-order speckle. In this paper, we take first steps in addressing the foregoing needs using a three-diffuser transmissive geometry that is a proxy for three-bounce NLoS imaging. In the small-diffusers limit, we show that the irradiance variances of cw and modulated nth-order speckle coincide and are (2 - 1)-times those of ordinary (first-order) speckle. The more important case for NLoS imaging, however, involves extended diffuse reflectors. For our transmissive geometry with extended diffusers, we treat third-order cw speckle and first-order modulated speckle. Our results there imply that speckle is unlikely to impede successful operation of coherent-illumination cw imagers, and they suggest that the same might be true for -field imagers.
PubMed: 32752478
DOI: 10.1364/OE.398226 -
Materials (Basel, Switzerland) Sep 2019The surface of Q235 low carbon steel was modified by the metal inert-gas welding (MIG) method; a 304 stainless steel surfacing layer was fabricated to improve the...
The surface of Q235 low carbon steel was modified by the metal inert-gas welding (MIG) method; a 304 stainless steel surfacing layer was fabricated to improve the properties of Q235 low carbon steel. For practical industry application, keyhole tungsten inter gas (K-TIG) welding was used to weld the surface-modified plates. The microstructure, elemental distribution, micro-hardness, and corrosion resistance of the surface-modified plates and the welded joints were analyzed. The corrosion tests of welded joints and surface-modified plates were carried out with the electrochemical method and hydrochloric acid immersion method, respectively, and surface morphology after corrosion was studied. The results show that the surface-modified plates and their welded joints were defect-free. The microstructure of the surfacing layer consisted of austenite, martensite, and ferrite; and the microstructure of the weld consisted mainly of martensite. The hardness and corrosion resistance of the surfacing layer was superior to that that of low carbon steel. The micro-hardness of the weld is higher than that of the stainless steel surfacing layer and the base material. The corrosion resistance of the surfacing layer is the best, and the corrosion resistance of the welding seam is better than that of the base material.
PubMed: 31489923
DOI: 10.3390/ma12182883 -
The Ocular Surface Jan 2010Mucins are major components in mucus secretions and apical cell membranes on wet-surfaced epithelia. Structurally, they are characterized by the presence of tandem... (Review)
Review
Mucins are major components in mucus secretions and apical cell membranes on wet-surfaced epithelia. Structurally, they are characterized by the presence of tandem repeat domains containing heavily O-glycosylated serine and threonine residues. O-glycans contribute to maintaining the highly extended and rigid structure of mucins, conferring to them specific physical and biological properties essential for their protective functions. at the ocular surface epithelia, mucin-type O-glycan chains are short and predominantly sialylated, perhaps reflecting specific requirements of the ocular surface. Traditionally, secreted mucins and their O-glycans in the tear film have been involved in the clearance of debris and pathogens from the surface of the eye. New evidence, however, shows that O-glycans on the cell-surface glycocalyx have additional biological roles in the protection of corneal and conjunctival epithelia, such as preventing bacterial adhesion, promoting boundary lubrication, and maintaining the epithelial barrier function through their interaction with galectin-3. Abnormalities in mucin-type O-glycosylation have been identified in many disorders where the stability of the ocular surface is compromised. This review summarizes recent advances in understanding the structure, biosynthesis, and function of mucin-type O-glycans at the ocular surface and their alteration in ocular surface disease.
Topics: Animals; Eye; Eye Diseases; Humans; Mucins; Tears
PubMed: 20105403
DOI: 10.1016/s1542-0124(12)70213-6 -
Advanced Drug Delivery Reviews Jan 2022Mucins are an ancient group of glycoproteins that provide viscoelastic, lubricating and hydration properties to fluids bathing wet surfaced epithelia. They are involved... (Review)
Review
Mucins are an ancient group of glycoproteins that provide viscoelastic, lubricating and hydration properties to fluids bathing wet surfaced epithelia. They are involved in the protection of underlying tissues by forming a barrier with selective permeability properties. The expression, processing and spatial distribution of mucins are often determined by organ-specific requirements that in the eye involve protecting against environmental insult while allowing the passage of light. The human ocular surface epithelia have evolved to produce an extremely thin and watery tear film containing a distinct soluble mucin product secreted by goblet cells outside the visual axis. The adaptation to the ocular environment is notably evidenced by the significant contribution of transmembrane mucins to the tear film, where they can occupy up to one-quarter of its total thickness. This article reviews the tissue-specific properties of human ocular mucins, methods of isolation and detection, and current approaches to model mucin systems recapitulating the human ocular surface mucosa. This knowledge forms the fundamental basis to develop applications with a promising biological and clinical impact.
Topics: Animals; Epithelial Cells; Eye; Humans; Mucins; Ocular Physiological Phenomena; Tears; Vision, Ocular
PubMed: 34875287
DOI: 10.1016/j.addr.2021.114074 -
Biosensors Apr 2018The present paper highlights the application of bacterial surface (S-) layer proteins as versatile components for the fabrication of biosensors. One technologically...
The present paper highlights the application of bacterial surface (S-) layer proteins as versatile components for the fabrication of biosensors. One technologically relevant feature of S-layer proteins is their ability to self-assemble on many surfaces and interfaces to form a crystalline two-dimensional (2D) protein lattice. The S-layer lattice on the surface of a biosensor becomes part of the interface architecture linking the bioreceptor to the transducer interface, which may cause signal amplification. The S-layer lattice as ultrathin, highly porous structure with functional groups in a well-defined special distribution and orientation and an overall anti-fouling characteristics can significantly raise the limit in terms of variety and the ease of bioreceptor immobilization, compactness of bioreceptor molecule arrangement, sensitivity, specificity, and detection limit for many types of biosensors. The present paper discusses and summarizes examples for the successful implementation of S-layer lattices on biosensor surfaces in order to give a comprehensive overview on the application potential of these bioinspired S-layer protein-based biosensors.
Topics: Biosensing Techniques; Humans; Membrane Glycoproteins; Protein S
PubMed: 29641511
DOI: 10.3390/bios8020040 -
BMC Ophthalmology Dec 2015Mucins locate to the apical surfaces of all wet-surfaced epithelia including ocular surface. The functions of the mucins include anti-adhesive, lubrication, water... (Review)
Review
Mucins locate to the apical surfaces of all wet-surfaced epithelia including ocular surface. The functions of the mucins include anti-adhesive, lubrication, water retention, allergens and pathogen barrier function. Ocular surface pathologies, i.e. dry eye syndrome or allergic conjunctivitis, are reportedly associated with alteration of expression pattern of mucin components. Recent investigations indicated anti-bacterial adhesion or anti-inflammatory effects of members of mucins in non-ocular tissues, i.e., gastrointestinal tracts or airway tissues, by using genetically modified mouse lines that lacks an expression of a mucin member. However, examination of ocular phenotypes of each of mucin gene-ablated mouse lines has not yet fully performed. Muc16-deficient mouse is associated with spontaneous subclinical inflammation in conjunctiva. The article reviews the roles of mucin members in modulation of local inflammation in mucous membrane tissues and phenotype of mouse lines with the loss of a mucin gene. Analysis of ocular surface of mucin-gene related mutant mouse lines are to be further performed.
Topics: Animals; Animals, Genetically Modified; Conjunctiva; Conjunctivitis; Cytokines; Dry Eye Syndromes; Epithelial Cells; Mice; Mucins; Tears
PubMed: 26818460
DOI: 10.1186/s12886-015-0137-5 -
Micromachines Feb 2021Underwater robots and vehicles have received great attention due to their potential applications in remote sensing and search and rescue. A challenge for micro aquatic...
Underwater robots and vehicles have received great attention due to their potential applications in remote sensing and search and rescue. A challenge for micro aquatic robots is the lack of small motors needed for three-dimensional locomotion in water. Here, we show a simple diving and surfacing device fabricated from thermo-sensitive poly(N-isopropylacrylamide) or a poly(N-isopropylacrylamide)-containing hydrogel. The poly(N-isopropylacrylamide)-containing device exhibited fast and reversible diving/surfacing cycles in response to changing temperature. Modulation of the interaction between poly(N-isopropylacrylamide) chains and water molecules at temperatures above or below the lower critical solution temperature regulates the gel density through the swelling and de-swelling. The gel surfaced in water when heated and sank when cooled. We further showed reversible diving/surfacing cycles of the device when exposed to electrical and ultrasonic stimuli. Finally, a small electrically heated gel was incorporated into a miniature submarine and used to control the diving depth. These results suggest that the poly(N-isopropylacrylamide)-containing device has good potential for underwater remote-controlled micro aquatic robots.
PubMed: 33669511
DOI: 10.3390/mi12020210 -
Molecules (Basel, Switzerland) Dec 2021Solid wettability is especially important for biomaterials and implants in the context of microbial adhesion to their surfaces. This adhesion can be inhibited by changes...
Effect of Spontaneous and Water-Based Passivation on Components and Parameters of Ti6Al4V (ELI Grade) Surface Tension and Its Wettability by an Aqueous Solution of Sucrose Ester Surfactants.
Solid wettability is especially important for biomaterials and implants in the context of microbial adhesion to their surfaces. This adhesion can be inhibited by changes in biomaterial surface roughness and/or its hydrophilic-hydrophobic balance. The surface hydrophilic-hydrophobic balance can be changed by the specifics of the surface treatment (proper conditions of surface preparation) or adsorption of different substances. From the practical point of view, in systems that include biomaterials and implants, the adsorption of compounds characterized by bacteriostatic or bactericidal properties is especially desirable. Substances that are able to change the surface properties of a given solid as a result of their adsorption and possess at least bacteriostatic properties include sucrose ester surfactants. Thus, in our studies the analysis of a specific surface treatment effect (proper passivation conditions) on a biomaterial alloy's (Ti6Al4V ELI, Grade 23) properties was performed based on measurements of the contact angles of water, formamide and diiodomethane. In addition, the changes in the studied solid surface's properties resulting from the sucrose monodecanoate (SMD) and sucrose monolaurate (SML) molecules' adsorption at the solid-water interface were also analyzed. For the analysis, the values of the contact angles of aqueous solutions of SMD and SML were measured at 293 K, and the surface tensions of the aqueous solutions of studied surfactants measured earlier were tested. From the above-mentioned tests, it was found that water environment significantly influences the components and parameters of Ti6Al4V ELI's surface tension. It also occurred that the addition of both SMD and SML to water (separately) caused a drop in the water contact angle on Ti6Al4V ELI's surface. However, the sucrose monolaurate surfactant is characterized by a slightly better tendency towards adsorption at the solid-water interface in the studied system compared to sucrose monodecanoate. Additionally, based on the components and parameters of Ti6Al4V ELI's surface tension calculated from the proper values of components and parameters of model liquids, it was possible to predict the wettability of Ti6Al4V ELI using the aqueous solutions of SMD and SML at various concentrations in the solution.
PubMed: 35011408
DOI: 10.3390/molecules27010179 -
Science and Technology of Advanced... Oct 2015Due to their excellent biodegradability characteristics, Mg and Mg-based alloys have become an emerging material in biomedical implants, notably for repair of bone as... (Review)
Review
Due to their excellent biodegradability characteristics, Mg and Mg-based alloys have become an emerging material in biomedical implants, notably for repair of bone as well as coronary arterial stents. However, the main problem with Mg-based alloys is their rapid corrosion in aggressive environments such as human bodily fluids. Previously, many approaches such as control of alloying materials, composition and surface treatments, have been attempted to regulate the corrosion rate. This article presents a comprehensive review of recent research focusing on surface treatment techniques utilised to control the corrosion rate and surface integrity of Mg-based alloys in both and environments. Surface treatments generally involve the controlled deposition of thin film coatings using various coating processes, and mechanical surfacing such as machining, deep rolling or low plasticity burnishing. The aim is to either make a protective thin layer of a material or to change the micro-structure and mechanical properties at the surface and sub-surface levels, which will prevent rapid corrosion and thus delay the degradation of the alloys. We have organised the review of past works on coatings by categorising the coatings into two classes-conversion and deposition coatings-while works on mechanical treatments are reviewed based on the tool-based processes which affect the sub-surface microstructure and mechanical properties of the material. Various types of coatings and their processing techniques under two classes of coating and mechanical treatment approaches have been analysed and discussed to investigate their impact on the corrosion performance, biomechanical integrity, biocompatibility and cell viability. Potential challenges and future directions in designing and developing the improved biodegradable Mg/Mg-based alloy implants were addressed and discussed. The literature reveals that no solutions are yet complete and hence new and innovative approaches are required to leverage the benefit of Mg-based alloys. Hybrid treatments combining innovative biomimetic coating and mechanical processing would be regarded as a potentially promising way to tackle the corrosion problem. Synergetic cutting-burnishing integrated with cryogenic cooling may be another encouraging approach in this regard. More studies focusing on rigorous testing, evaluation and characterisation are needed to assess the efficacy of the methods.
PubMed: 27877829
DOI: 10.1088/1468-6996/16/5/053501