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Balkan Medical Journal Mar 2023Various studies have reported the effects of testosterone on different cell types, yet bone marrow-derived mesenchymal stem cells’ cellular responses to testosterone...
Testosterone Propionate Promotes Proliferation and Viability of Bone Marrow Mesenchymal Stem Cells while Preserving Their Characteristics and Inducing Their Anti-Cancer Efficacy.
BACKGROUND
Various studies have reported the effects of testosterone on different cell types, yet bone marrow-derived mesenchymal stem cells’ cellular responses to testosterone remain unknown.
AIMS
To investigate the effects of testosterone propionate, an oil-soluble short-acting form of testosterone, on human bone marrow-derived mesenchymal stem cells’ proliferation and viability after 24 hours of incubation. We also investigated the impact of testosterone propionate on bone marrow-derived mesenchymal stem cell’s polarization and cytotoxicity on K562 leukemia cell line.
STUDY DESIGN
In vitro study.
METHODS
We expanded commercially available bone marrow derived mesenchymal stem cells in vitro and treated them with testosterone propionate at concentrations ranging from 10-10 M for 24 hours. Ideal concentration was determined by evaluating cellular viability and proliferation with Annexin V/Propidium Iodide assay and carboxyfluorescein succinimidyl ester staining. The characteristic features of bone marrow-derived mesenchymal stem cells were evaluated by immunophenotyping and investigating their differentiation capacities. Bone marrow-derived mesenchymal stem cells’ cytotoxic properties upon testosterone propionate treatment were determined by co-culturing the cells with K562 cells and with confocal imaging investigating polarization.
RESULTS
Testosterone propionate promoted proliferation and maintained the viability of bone marrow-derived mesenchymal stem at 10 M concentration. Further evaluations were conducted with the determined dose. The results showed that, apart from promoting mesenchymal stem cells’ polarization and increasing their cytotoxicity on K562 cells, testosterone propionate did not alter differentiation capacities of bone marrow-derived mesenchymal stem cells and certain cell surface markers, but led to a significant increase in HLA-DR expression.
CONCLUSION
The findings reveal that testosterone propionate promotes the proliferation and survival of bone marrow-derived mesenchymal stem cells in a dose-dependent manner without hampering their differentiation capacities, induces their polarization to the pro-inflammatory phenotype, and increases their cytotoxicity on the K562 cell line.
Topics: Humans; Testosterone Propionate; Mesenchymal Stem Cells; Cell Differentiation; Neoplasms; Cell Proliferation
PubMed: 36748249
DOI: 10.4274/balkanmedj.galenos.2022.2022-10-21 -
Biological & Pharmaceutical Bulletin Jan 2019Veratrum maackii (VM), a perennial plant in the Melanthiaceae family, has anti-hypertensive, anti-cholinergic, anti-asthmatic, anti-tussive, anti-fungal,...
Veratrum maackii (VM), a perennial plant in the Melanthiaceae family, has anti-hypertensive, anti-cholinergic, anti-asthmatic, anti-tussive, anti-fungal, anti-melanogenesis, and anti-tumor activities. Here, we investigated the therapeutic effect of VM on benign prostatic hyperplasia (BPH) in human normal prostate cell line (WPMY-1) and a testosterone propionate-induced BPH animal model. WPMY-1 cells were treated with VM (1-10 µg/mL) and testosterone propionate (100 nM). BPH in rats was generated via daily subcutaneous injections of testosterone propionate (3 mg/kg) dissolved in corn oil, for 4 weeks. VM (150 mg/kg) was administered daily for 4 weeks by oral gavage concurrently with the testosterone propionate. All rats were sacrificed and the prostates were dissected, weighed, and subjected to histological, immunohistochemical, and biochemical examinations. Immunoblotting experiments indicated that WPMY-1 cells treated testosterone propionate had increased expression of prostate specific antigen (PSA) and androgen receptor (AR), and treatment with VM or finasteride blocked this effect. In rat model, VM significantly reduced prostate weight, prostatic hyperplasia, prostatic levels of dihydrotestosterone (DHT), and expression of proliferation markers such as proliferating cell nuclear antigen (PCNA) and cyclin D1, but increased the expression of pro-apoptotic Bcl-2-associated X protein (Bax) and the cleavage of caspase-3. VM administration also suppressed the testosterone propionate-induced activation of nuclear factor-kappaB (NF-κB). Our results indicate that VM effectively represses the development of testosterone propionate-induced BPH, suggesting it may be a useful treatment agent for BPH.
Topics: Animals; Cell Line; Cell Survival; Dose-Response Relationship, Drug; Humans; Male; Plant Extracts; Prostatic Hyperplasia; Rats; Rats, Sprague-Dawley; Testosterone Propionate; Treatment Outcome; Veratrum
PubMed: 30381617
DOI: 10.1248/bpb.b18-00313 -
Current Drug Discovery Technologies 2021Benign prostate hyperplasia [BPH] is an abnormal growth of prostate observed commonly in elderly males. Artemisinin has been reported to reduce the levels of...
BACKGROUND
Benign prostate hyperplasia [BPH] is an abnormal growth of prostate observed commonly in elderly males. Artemisinin has been reported to reduce the levels of testosterone.
OBJECTIVE
This study is designed to evaluate the efficacy of Artemisinin on testosterone propionate [TP] induced benign prostate hyperplasia.
MATERIALS AND METHODS
Male Wistar albino rats [n=24] were separated into four groups of six rats each. Group I served as control and distilled water using tween 80 as an emulsifying agent was administered subcutaneously. BPH was induced by testosterone propionate 3mg/kg [Group II], S.C. daily for 28 days. Group III was BPH + Finasteride treated group (10mg/kg orally for 28 days) and BPH + Artemisinin treated group (Group IV) (50 mg/kg orally for 28 days).
RESULT
The study results showed significantly high levels of serum prostatic acid phosphatase (PAP), lactate dehydrogenase (LDH) and an elevation in prostate weight and prostatic index in Group II (BPH) when compared with Group I. The histopathological examination showed an increase in the epithelial proliferation of prostatic cells with involutions protruding into the lumen in BPH group when compared to the normal group. Treatment with Artemisinin (50 mg/kg) reduced the levels of PAP, LDH, prostate weight and prostatic index to a significant extent and restored the histoarchitectural features of the cells.
CONCLUSION
The present study concludes that Artemisinin is efficacious in testosterone propionate induced BPH. This could be attributed, at least partly, to its anti-inflammatory property or its role in testosterone level reduction or as a Vitamin D receptor modulator.
Topics: Animals; Anti-Inflammatory Agents; Artemisinins; Disease Models, Animal; Humans; Male; Prostate; Prostatic Hyperplasia; Rats; Rats, Wistar; Testosterone Propionate
PubMed: 32532194
DOI: 10.2174/1570163817666200612151150 -
Journal of Tissue Engineering and... Sep 2019Peripheral human nerves fail to regenerate across long tube implants (>2 cm), and tissue-engineered nerve grafts represent a promising treatment alternative. The...
Peripheral human nerves fail to regenerate across long tube implants (>2 cm), and tissue-engineered nerve grafts represent a promising treatment alternative. The present study aims to investigate the testosterone propionate (TP) repair effect of acellular nerve allograft (ANA) seeded with allogeneic bone marrow mesenchymal stem cells (BMSCs) on 3-cm canine sciatic nerve defect. ANA cellularized with allogeneic BMSCs was implanted to the defect, and TP was injected into the lateral crus of the defected leg. The normal group, the autograft group, the ANA + BMSCs group, the ANA group, and the nongrafted group were used as control. Five months postoperatively, dogs in the TP + ANA + BMSCs group were capable of load bearing, normal walking, and skipping, the autograft group and the ANA + BMSCs group demonstrated nearly the same despite a slight limp. The compound muscle action potentials (CMAPs) on the injured side to the uninjured site in the TP + ANA + BMSCs group were significantly higher than that in the ANA + BMSCs group [CMAPs ratio at A: F(3, 20) = 191.40; 0.02, CMAPs ratio at B: F(3, 20) = 43.27; 0.01]. Masson trichrome staining revealed that in the TP + ANA + BMSCs group, both the diameter ratio of the myelinated nerve and the thickness ratio of regenerated myelin sheath were significantly larger than that in the other groups [the diameter of myelinated nerve fibers: F(3, 56) = 13.45; P < .01, the thickness ratio of regenerated myelin sheath: F(3, 56) = 51.25; P < .01]. In conclusion, TP could significantly increase the repairing effects of the ANA + BMSCs group, and their combination was able to repair 3-cm canine sciatic nerve defect. It therefore represents a promising therapeutic approach.
Topics: Allografts; Animals; Cell Separation; Dogs; Electrophysiological Phenomena; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; Muscles; Nerve Regeneration; Sciatic Nerve; Testosterone Propionate; Tissue Engineering
PubMed: 31267700
DOI: 10.1002/term.2922 -
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue Dec 2020To investigate the effect of testosterone propionate injection on the condition and prognosis of patients with sepsis.
OBJECTIVE
To investigate the effect of testosterone propionate injection on the condition and prognosis of patients with sepsis.
METHODS
The clinical data of 61 sepsis patients admitted to the department of intensive care medicine, Weinan Central Hospital from June 2009 to October 2019 were retrospectively analyzed. All patients were treated with anti-infection, control of infection sources, organ function support, nutrition enhancement and supportive treatment. On the basis of routine treatment, observation group was given 100 mg of testosterone propionate injection for deep intramuscular injection twice a week (twice in total), and control group was not given testosterone propionate injection. The general information and laboratory indexes before treatment were observed, and the testosterone, albumin, acute physiology and chronic health evaluation II (APACHE II), sequential organ failure assessment (SOFA) score after treatment, intensive care unit (ICU) hospitalization time, total hospitalization cost, mechanical ventilation time, 28-day all-cause mortality and other indicators of the patients in two groups were compared.
RESULTS
There were no significant differences between the two groups in gender, age and other baseline data and laboratory indexes before treatment. After treatment, in observation group the testosterone (μg/L: 3.69±2.38 vs. 2.85±0.90) and albumin (g/L: 39.87±1.98 vs. 26.25±4.13) were significantly higher than those in control group. Total hospitalization expenses (ten thousand Yuan: 10.14±3.22 vs. 12.10±3.91), APACHE II (13.71±2.13 vs. 23.23±2.52), SOFA (4.45±1.57 vs. 9.97±2.65), ICU hospitalization time (days: 12.36±4.37 vs. 14.03±3.86) and mechanical ventilation time (days: 3.00±1.85 vs. 7.00±2.50) were significantly lower than those in control group (all P < 0.05), and the difference in 28-day all-cause mortality of two groups was not significant [3.2% (1/31) vs. 13.3% (4/30), P > 0.05].
CONCLUSIONS
Testosterone propionate injection can increase albumin level, shorten the time of mechanical ventilation, and improve the condition and prognosis of patients with sepsis.
Topics: APACHE; Humans; Intensive Care Units; Prognosis; ROC Curve; Retrospective Studies; Sepsis; Testosterone Propionate
PubMed: 33541496
DOI: 10.3760/cma.j.cn121430-20200429-00611 -
Fish Physiology and Biochemistry Dec 2022The objective of this work was to develop a food additive for the sex reversal of Nile tilapia (Oreochromis niloticus) based on a simple oil in water (O/W) nanoemulsion...
The objective of this work was to develop a food additive for the sex reversal of Nile tilapia (Oreochromis niloticus) based on a simple oil in water (O/W) nanoemulsion with testosterone propionate for incorporation into commercial feed. Oil screening and evaluation of the organoleptic and physicochemical characteristics were carried out to determine the best formulation. A palatability test was also performed. Sex reversal test was assayed using 5 experimental groups: negative control - macerated feed without hormone; free testosterone - macerated feed with 60 mg/kg of testosterone propionate diluted in ethanol; and macerated feed with testosterone propionate nanoemulsion at a concentration of 30, 60, and 90 mg/kg. Stable nanoemulsions (size 76-210 nm) with testosterone propionate were produced. All nanoemulsion-added feed was palatable to tilapia. We obtained sex reversal values of ≈65, 75, and 72% in the groups of 30, 60, and 90 mg/kg, respectively. We can conclude that the nanoemulsion showed promising results; it is capable of inducing sex reversal in tilapia, is suitable as a commercial product, and has the potential to promote safety for rural staff and reduce the environmental impact of hormones.
Topics: Animals; Cichlids; Testosterone; Testosterone Propionate; Tilapia; Animal Feed
PubMed: 36480096
DOI: 10.1007/s10695-022-01156-3 -
JAMA Jun 1964
Topics: Biomedical Research; Breast Neoplasms; Castration; Female; Humans; Menopause; Neoplasms; Ovary; Prognosis; Testosterone; Testosterone Propionate
PubMed: 14151168
DOI: 10.1001/jama.1964.03060380037009 -
Tissue & Cell Apr 2022To investigate the effects of the previous administration of testosterone propionate (TP) on the morphology of the gastrocnemius muscle of Wistar rats submitted to...
To investigate the effects of the previous administration of testosterone propionate (TP) on the morphology of the gastrocnemius muscle of Wistar rats submitted to ladder-based resistance training (LRT). Twenty-eight rats were divided equally into groups: initial control (CI), 4-week TP (CT4), 4-week TP + LRT (TRT), and placebo + LRT (RT). The rats from the CT4 and TRT groups were treated with TP for four weeks (10 mg/kg/week). TRT and RT trained for ten weeks. The rodents were euthanized at the end of the experiment, and gastrocnemius muscle, prostate, and left and right testicles were collected. There was no statistical difference between the RT and TRT for final volume load. The prostate mass of the TRT and RT groups was statistically heavier than the CT4 group (P < 0.01). The TRT group's prostate/body mass ratio was statistically different from the CT4 group (P < 0.05). The TRT group was shown to have larger type I, type II, and mean fCSA fibers than all other groups (P < 0.001). Regarding the nuclei/fiber ratio (N/f), the CT4, RT, and TRT groups had higher values than CI (P < 0.01). In addition, the RT group showed a higher N/f ratio than CT4 (P < 0.001) but lower than TRT (P < 0.001). In conclusion, short-term TP administration before resistance training can elicit a greater N/f ratio and size of the mean fCSA of the Gastrocnemius muscle of young adult Wistar rats than resistance training alone.
Topics: Animals; Humans; Hypertrophy; Male; Muscle, Skeletal; Rats; Rats, Wistar; Resistance Training; Testosterone; Testosterone Propionate
PubMed: 35074725
DOI: 10.1016/j.tice.2022.101741 -
The Journal of Clinical Endocrinology... Dec 1945
Topics: Androgens; Hormone Replacement Therapy; Pruritus; Testosterone Propionate
PubMed: 21010989
DOI: 10.1210/jcem-5-10-412 -
Clinical Science Feb 1957
Topics: Humans; Kidney Diseases; Renal Insufficiency; Testosterone; Testosterone Propionate
PubMed: 13414136
DOI: No ID Found