-
Scientific Reports Sep 2022Animal experiments play an essential role in advances in the research and treatment of burns. Currently, researchers often rely on personal experience or the literature...
Animal experiments play an essential role in advances in the research and treatment of burns. Currently, researchers often rely on personal experience or the literature to complete the construction of animal disease models, which may lead to a lack of scientific rigor and a wide range of animal disease models with reference value. The purposes of this study were to establish a third-degree burn model of rats using the Delphi method to provide a reference. Two rounds of a Delphi expert consultation survey were conducted on experts (n = 13) in this study, and then the boundary value method was used to screen, modify and supplement the indicators. Next, the indicator weight was determined by dividing the boundary value, and finally, the index system of the rat model of third-degree burns was established. The statistical analysis results show that the positive coefficients of the two rounds of expert consultation are 100% and 88.67% respectively. The expert authority coefficient values were 0.73 and 0.67, respectively, and the expert coordination coefficient test was P < 0.001. According to the experts' suggestion, the third-degree burn model of rats with 8 first-degree indexes, 14 second-degree indexes and 46 third-degree indexes was finally established. According to the characteristics and quality requirements of animal models, this study constructs a rat model of third degree burns, which is expected to expressively improve the overall proficiency of burn research quality.
Topics: Animals; Burns; Delphi Technique; Disease Models, Animal; Rats
PubMed: 36056098
DOI: 10.1038/s41598-022-18092-0 -
Growth Factors (Chur, Switzerland) Dec 2020Fibroblast growth factor 2 (FGF-2) is a multifunctional protein that has major roles in wound healing, tissue repair, and regeneration. This therapeutic protein is...
Fibroblast growth factor 2 (FGF-2) is a multifunctional protein that has major roles in wound healing, tissue repair, and regeneration. This therapeutic protein is widely used for burn treatment because it can stimulate cell proliferation and differentiation, angiogenesis, and extracellular matrix remodeling. In this study, we developed a simple method using a controlled heated brass rod to create a homogenous third-degree burn murine model and evaluated the treatment using recombinant human FGF-2 (rhFGF-2). The results indicated that the wound area was 0.83 ± 0.05 cm and wound depth was 573.42 ± 147.82 μm. Mice treated with rhFGF-2 showed higher rates of wound closure, granulation tissue formation, angiogenesis, and re-epithelialization than that of phosphate-buffered saline (PBS)-treated group. In conclusion, our lab-made rhFGF-2 could be a potentially therapeutic protein for burn treatment as well as a bioequivalent drug for other commercial applications using FGF-2.
Topics: Animals; Burns; Cell Proliferation; Fibroblast Growth Factor 2; Humans; Mice; Neovascularization, Pathologic; Wound Healing
PubMed: 34415815
DOI: 10.1080/08977194.2021.1967342 -
Burns : Journal of the International... Jun 2022Topical drug therapy is one of the most effective approaches in third-degree burn wound treatments. To optimize and enhance drug permeation through burn eschar, we need...
Topical drug therapy is one of the most effective approaches in third-degree burn wound treatments. To optimize and enhance drug permeation through burn eschar, we need to characterize this barrier, most importantly, its affinity to drugs; the subject of this investigation. Hansen Solubility Parameters (HSP), as polarity and affinity scale, were measured here for human third-degree burn eschar through uptake studies using 19 solvents at 25 °C and 32 °C and two hydration levels by gravimetric method combined with thermal analysis and Karl Fischer titration. HSP parameters of dispersion (δ), bipolar (δ), and hydrogen bonding (δ) were calculated by HSPiP software. Results showed δ, δ and δ of 17.0, 12.5, 14.6 and 16.8, 12.4, 14.4 at 25 and 32 °C respectively for normally-hydrated samples. Full hydration increased HSP values to 17.2, 12.9, 15.3 (25 °C) and 17.1, 12.8, 15.1 (32 °C). Good correlations between solvents uptakes and HSP values were observed for all parameters; higher for δ. Increased temperature decreased them with more changes in δ. Relative Energy Differences (RED) were calculated and shown to be a good parameter for predicting drug-eschar affinity. The obtained information is useful for drug selection and carrier design in drug delivery through burn eschar.
Topics: Burns; Excipients; Humans; Pharmaceutical Preparations; Soft Tissue Injuries; Solubility; Solvents
PubMed: 34893367
DOI: 10.1016/j.burns.2021.07.017 -
Journal of Magnetic Resonance Imaging :... Sep 2023
Topics: Humans; Oximetry; Burns; Oxygen
PubMed: 36574217
DOI: 10.1002/jmri.28557 -
Trends in Biotechnology Apr 2024Technological advances have increasingly provided more and better treatment options for patients with severe burns. Here, we provide a bird's-eye view of the product...
Technological advances have increasingly provided more and better treatment options for patients with severe burns. Here, we provide a bird's-eye view of the product development process for third-degree burn wounds with considerations of the critical interaction with regulatory bodies, existing technological gaps, and future directions for skin substitutes.
Topics: Humans; Skin, Artificial; Skin Transplantation; Burns; Skin
PubMed: 37949776
DOI: 10.1016/j.tibtech.2023.10.005 -
Wound Management & Prevention Dec 2021Many patients who have diabetes and peripheral neuropathy wear compression socks, which are widely available and may be purchased with a copper component. There is also...
BACKGROUND
Many patients who have diabetes and peripheral neuropathy wear compression socks, which are widely available and may be purchased with a copper component. There is also a well-documented history of patients with neuropathy developing thermal burns from heat sources. Patients with diabetes are at an increased risk of complications when they sustain burns.
PURPOSE
To describe a patient with diabetes and neuropathy who developed third-degree burns while wearing a copper-containing compression sock.
CASE REPORT
A 68-year-old man with type 2 diabetes and peripheral neuropathy wore a copper-containing compression sock while sitting in the sun for several hours. Afterward, he noted severe blistering and was ultimately diagnosed with several areas of second- and third-degree burns. Wound treatment included sharp debridement, dressing management, and tubular compression. All wounds were healing well when the patient was lost to follow-up.
CONCLUSION
This case report illustrates the risk of burn injury when wearing compression socks infused with copper in a situation in which the sock could absorb heat, especially in persons with neuropathy. This report and previous studies showing the increased risk of burn injury complications in persons with diabetes underscore the importance of burn prevention education in this population. Future research to examine the thermal conductivity of metal-containing clothing is recommended.
Topics: Aged; Burns; Copper; Diabetes Mellitus, Type 2; Humans; Lower Extremity; Male; Peripheral Nervous System Diseases
PubMed: 35030541
DOI: No ID Found -
Journal of the American College of... Apr 2018The standard of burn treatment today reflects major advances. We sought to quantitate the impact of these advances on burn survival via age-stratified mortality ratios...
BACKGROUND
The standard of burn treatment today reflects major advances. We sought to quantitate the impact of these advances on burn survival via age-stratified mortality ratios compared with other reported mortality analyses in burns.
STUDY DESIGN
Age, percent of the total body surface area (TBSA) burned, presence of inhalation injury, length of stay, and survival status were recorded at admission and at discharge for all new burn admissions between 1989 and 2017. The expected mortality probability was calculated using historical multiple regression techniques and compared with observed data. We developed a prediction model for our observed data.
RESULTS
Between 1989 and 2017, there were 10,384 consecutive new burn admissions, with 355 mortalities (median age, 13 years; median percent TBSA burn, 11%). We saw a significant decrease in our observed mortality data compared to historical predictions (p < 0.0001), and a 2% reduction per year in mortality during the 3 decades. The prediction model of mortality for the data is as follows: Pr(dying) = e/(1 + e) where x = -6.44 - 0.12 age + 0.0042 age - 0.0000283 age + 0.0499 TBSA + 1.21 Inhalation Injury + 0.015 third degree TBSA.
CONCLUSIONS
The reduction in mortality over time may be attributed to successful changes in standard of care protocols in the burn center that improved the outlook for burned individuals, including protocols for management of inhalation injury, nutrition, resuscitation, and early excision and grafting.
Topics: Adolescent; Adult; Age Factors; Body Surface Area; Burns; Child; Child, Preschool; Female; Humans; Infant; Infant, Newborn; Length of Stay; Male; Middle Aged; Retrospective Studies; Survival Rate; Young Adult
PubMed: 29530306
DOI: 10.1016/j.jamcollsurg.2017.12.045 -
Annals of Plastic Surgery Jul 2019There have been significant advances in the care of burns over the past decade. As a result of the improved survival of burn patients, attention has shifted to the... (Review)
Review
There have been significant advances in the care of burns over the past decade. As a result of the improved survival of burn patients, attention has shifted to the optimized management of their wounds. Traditionally, autografts have been described as the gold standard treatment in cases of deep second- and third-degree burn wounds; however, they are limited especially in large surface area burns. As such, advancements have been made in the development of biologic dressings, which attempt to mimic the function of the lost epidermis and/or dermis. The ideal biologic dressing is nontoxic, lacks antigenicity, is immunologically compatible, and is sterile. Additionally, easy storage conditions, long shelf lives, and reasonable costs are key determinants of whether biologic dressings may truly be widely used in the clinical setting. Biologic dressings serve an important role as skin substitutes in the setting of acute burn injury. This review aims to summarize the multitude of available biologic dressings and their applications. METHODS: The PubMed and Google Scholar databases were searched for the following terms either alone or in combination: "burn injury," "biologic membrane," "skin substitutes," "biosynthetic dressings," and "acellular membrane."
Topics: Biological Dressings; Biological Products; Burns; Female; Humans; Injury Severity Score; Male; Prognosis; Skin Transplantation; Skin, Artificial; Transplantation, Autologous; Wound Healing
PubMed: 31194708
DOI: 10.1097/SAP.0000000000001915 -
Pain Medicine (Malden, Mass.) May 2018
Topics: Burns; Catheter Ablation; Humans; Knee Joint; Male; Middle Aged; Osteoarthritis, Knee; Radiofrequency Ablation; Treatment Outcome
PubMed: 29025030
DOI: 10.1093/pm/pnx204 -
Lasers in Medical Science Apr 2019This systematic review was performed to identify the role of photobiomodulation therapy in experimental models of third-degree burns used to induce oxidative stress....
This systematic review was performed to identify the role of photobiomodulation therapy in experimental models of third-degree burns used to induce oxidative stress. EMBASE, PubMed, and CINAHL databases were searched for studies published between January 2003 and January 2018 on the topics of photobiomodulation therapy and third-degree burns. Any study that assessed the effects of photobiomodulation therapy in animal models of third-degree burns was included in the analysis. A total of 17 studies were selected from 1182 original articles targeted on photobiomodulation therapy and third-degree burns. Two independent raters with a structured tool for rating the research quality critically assessed the articles. Although the small number of studies limits the conclusions, the current literature research indicates that photobiomodulation therapy can be an effective short-term approach to accelerate the healing process of third-degree burns, to increase and modulate the inflammatory process, to accelerate the proliferation of fibroblasts, and to enhance the quality of the collagen network. However, differences still exist in the terminology used to describe the parameters and the dose of photobiomodulation therapy.
Topics: Animals; Burns; Disease Models, Animal; Journal Impact Factor; Low-Level Light Therapy; Publication Bias; Risk Factors
PubMed: 30229346
DOI: 10.1007/s10103-018-2633-3