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Antonie Van Leeuwenhoek Nov 2022Two strains, designated as Marseille-P2918 and Marseille-P3646, were isolated from a 14-week-old Senegalese girl using culturomics: Urmitella timonensis strain...
Draft genomes and descriptions of Urmitella timonensis gen. nov., sp. nov. and Marasmitruncus massiliensis gen. nov., sp. nov., isolated from severely malnourished African children using culturomics.
Two strains, designated as Marseille-P2918 and Marseille-P3646, were isolated from a 14-week-old Senegalese girl using culturomics: Urmitella timonensis strain Marseille-P2918 (= CSUR P2918, = DSM 103634) and Marasmitruncus massiliensis strain Marseille-P3646 (= CSUR P3646, = CCUG72353). Both strains were rod-shaped, anaerobic, spore forming motile bacteria. The 16S rRNA gene sequences of strains Marseille-P2918 (LT598554) and Marseille-P3646 (LT725660) shared 93.25% and 94.34% identity with Tissierella praeacuta ATCC 25539 and Anaerotruncus colihominis CIP 107754, their respective phylogenetically closest species with standing in nomenclature. Therefore, strain Marseille-P2918 is classified within the family Tissierellaceae and order Tissierellales whereas strain Marseille-P3646 is classified within the family Oscillospiraceae and order Eubacteriales. The genome of strain Marseille-P2918 had a size of 2.13 Mb with a GC content of 50.52% and includes six scaffolds and six contigs, and that of strain Marseille-P3646 was 3.76 Mbp long consisting of five contigs with a 50.04% GC content. The genomes of both strains presented a high percentage of genes encoding enzymes involved in genetic information and processing, suggesting a high growth rate and adaptability. These new taxa are extensively described and characterised in this paper, using the concept of taxono-genomic description.
Topics: Humans; Child; Female; RNA, Ribosomal, 16S; DNA, Bacterial; Phylogeny
PubMed: 36149539
DOI: 10.1007/s10482-022-01777-x -
Cureus Apr 2022is a gram negative anaerobe with few documented cases of human infections. This case illustrates a patient who was admitted for infected chronic sacral and ischial...
is a gram negative anaerobe with few documented cases of human infections. This case illustrates a patient who was admitted for infected chronic sacral and ischial decubitus wounds. BACT/ALERT blood culture system using Matrix Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) (Bruker Daltonics, Billerica, USA) was employed ultimately identifying as the causative organism. The suspected source of the pathogen was presumed to be from his infected decubitus wounds.
PubMed: 35509737
DOI: 10.7759/cureus.23745 -
BMJ Case Reports Jan 2017The Gram-negative, rod-shaped, anaerobe bacteria Tissierella praeacuta was first described in 1908 by Tissier. However, during the past decade, Clostridium hastiforme...
The Gram-negative, rod-shaped, anaerobe bacteria Tissierella praeacuta was first described in 1908 by Tissier. However, during the past decade, Clostridium hastiforme has been recognised as a later synonym of T. praeacuta. C. hastiforme/T. praeacuta has only rarely been described in previous literature as a cause of human infection. We present here a case report of C. hastiforme/T. praeacuta bacteraemia secondary to pyometra in a 64-year-old woman with a history of multiple sclerosis and an intrauterine device inserted three decades earlier. C. hastiforme/T. praeacuta was isolated from blood as well as pus from the site of infection. The patient was cured with a combination of drainage and antibiotic therapy.
Topics: Bacteremia; Clostridium Infections; Device Removal; Drainage; Female; Humans; Intrauterine Devices; Middle Aged; Prosthesis-Related Infections; Pyometra
PubMed: 28100574
DOI: 10.1136/bcr-2016-218084 -
IDCases 2022Tissierella praeacuta, also known as Clostridium hastiforme is an anaerobic gram negative bacteria, first isolated in 1908, by P.H. Tissier. To date, there are currently...
Tissierella praeacuta, also known as Clostridium hastiforme is an anaerobic gram negative bacteria, first isolated in 1908, by P.H. Tissier. To date, there are currently six documented cases of this environmental organism causing infection in humans. Here, we present a patient who was admitted to hospital with osteomyelitis of his right calcaneus, found to subsequently have T. praeacuta bacteremia isolated from anaerobic blood cultures. During his inpatient course, he was treated with IV vancomycin, cefepime, and metronidazole in addition to surgical debridement of his foot wound. The patient was discharged on a course of oral Levofloxacin and Amoxicillin-Clavulanate with significant clinical improvement.
PubMed: 35127451
DOI: 10.1016/j.idcr.2022.e01425 -
Cureus Sep 2023is a rare gram-variable bacillus that naturally occurs in the environment and is pathogenic in humans with chronic infections. We report the case of a 45-year-old man...
is a rare gram-variable bacillus that naturally occurs in the environment and is pathogenic in humans with chronic infections. We report the case of a 45-year-old man with a history of chronic osteomyelitis of the left tibia and recurrent bacteremia secondary to intravenous drug use (IVDU). He had previously received multiple partially completed courses of antibiotics over the past one year. Blood cultures demonstrated polymicrobial infection, including and methicillin-sensitive managed with parenteral beta-lactams, and the subsequent first surveillance cultures remained sterile. Medical literature on human infections with is limited due to its rare occurrence. Most cases have reported sensitivity to beta-lactam antibiotics, making them an antibiotic of choice. infections should prompt a search for additional underlying infectious foci and treatment of any additional co-infecting microbes.
PubMed: 37818513
DOI: 10.7759/cureus.44962 -
Clinical Microbiology and Infection :... Oct 2005The in-vitro activities of penicillin, ticarcillin-clavulanic acid, cefoxitin, imipenem, ertapenem, metronidazole and clindamycin were evaluated against 138...
The in-vitro activities of penicillin, ticarcillin-clavulanic acid, cefoxitin, imipenem, ertapenem, metronidazole and clindamycin were evaluated against 138 Gram-negative anaerobic isolates (82 Bacteroides fragilis group, 17 non-fragilis Bacteroides spp., 31 Prevotella spp., four Fusobacterium spp., two Veillonella spp., one Porphyromonas sp. and one Tissierella praeacuta) collected from six general hospitals in Athens, Greece. Overall rates of non-susceptibility (both resistant and intermediately-resistant) to penicillin and ticarcillin-clavulanic acid were 81.8% and 2.3%, respectively. The rates of non-susceptibility to cefoxitin and clindamycin were 30.3% and 31.1%, respectively, and that for metronidazole was 4.3% (four Prevotella spp. isolates, one Porphyromonas sp. isolate and one B. fragilis isolate). Only the single B. fragilis isolate was nim-positive by PCR. Only one B. fragilis isolate was resistant to both carbapenems tested, while six more Bacteroides spp. isolates were imipenem-susceptible and ertapenem-non-susceptible. The MIC range, MIC(50) and MIC(90) values were comparable for imipenem and ertapenem, although ertapenem MIC(90)s were one or two two-fold dilutions higher.
Topics: Anti-Bacterial Agents; Bacteria, Anaerobic; Bacteroides fragilis; Carbapenems; Drug Resistance, Microbial; Ertapenem; Gram-Negative Anaerobic Bacteria; Gram-Negative Bacterial Infections; Greece; Humans; Inhibitory Concentration 50; Metronidazole; Microbial Sensitivity Tests; beta-Lactams
PubMed: 16153256
DOI: 10.1111/j.1469-0691.2005.01233.x -
Cureus Jul 2023We report a case of bacteremia and septic thrombophlebitis of the ovarian vein as a rare puerperal complication in a young patient. She was successfully managed with...
We report a case of bacteremia and septic thrombophlebitis of the ovarian vein as a rare puerperal complication in a young patient. She was successfully managed with subcutaneous low molecular weight heparin (LMWH) and intravenous (IV) antibiotics before transitioning to a prolonged course of oral antibiotics at discharge.
PubMed: 37621834
DOI: 10.7759/cureus.42385 -
The Canadian Journal of Infectious... 2009Brain abscess, while rare, confers high mortality, especially in the developing world. The case of a Ugandan child with a polymicrobial brain abscess including infection...
Brain abscess, while rare, confers high mortality, especially in the developing world. The case of a Ugandan child with a polymicrobial brain abscess including infection with Tissierella praeacuta/Clostridium hastiforme requiring repeated drainage and eventual surgical excision is reported. The case demonstrates the importance of considering anaerobic organisms in the treatment of children with brain abscess from the developing world.
PubMed: 20808460
DOI: 10.1155/2009/407139