Did you mean: trabulsiella guianensis
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Journal of Bacteriology Jun 2019is a nonpathogenic enterobacterium that was isolated from a vacuum cleaner on the island of Guam. It has one H-oxidizing Hyd-2-type hydrogenase (Hyd) and encodes an...
Dissection of the Hydrogen Metabolism of the Enterobacterium Trabulsiella guamensis: Identification of a Formate-Dependent and Essential Formate Hydrogenlyase Complex Exhibiting Phylogenetic Similarity to Complex I.
is a nonpathogenic enterobacterium that was isolated from a vacuum cleaner on the island of Guam. It has one H-oxidizing Hyd-2-type hydrogenase (Hyd) and encodes an H-evolving Hyd that is most similar to the uncharacterized formate hydrogenlyase (FHL-2 ) complex. The FHL-2 (FHL-2 ) complex is predicted to have 5 membrane-integral and between 4 and 5 cytoplasmic subunits. We showed that the FHL-2 complex catalyzes the disproportionation of formate to CO and H FHL-2 has activity similar to that of the FHL-1 complex in H evolution from formate, but the complex appears to be more labile upon cell lysis. Cloning of the entire 13-kbp FHL-2 operon in the heterologous host has now enabled us to unambiguously prove FHL-2 activity, and it allowed us to characterize the FHL-2 complex biochemically. Although the formate dehydrogenase (FdhH) gene is not contained in the operon, the FdhH is part of the complex, and FHL-2 activity was dependent on the presence of FdhH. Also, in contrast to , can ferment the alternative carbon source cellobiose, and we further investigated the participation of both the H-oxidizing Hyd-2 and the H-forming FHL-2 under these conditions. Biological H production presents an attractive alternative for fossil fuels. However, in order to compete with conventional H production methods, the process requires our understanding on a molecular level. FHL complexes are efficient H producers, and the prototype FHL-1 complex in is well studied. This paper presents the first biochemical characterization of an FHL-2-type complex. The data presented here will enable us to solve the long-standing mystery of the FHL-2 complex, allow a first biochemical characterization of 's fermentative metabolism, and establish this enterobacterium as a model organism for FHL-dependent energy conservation.
Topics: Bacterial Proteins; Catalysis; Enterobacteriaceae; Fermentation; Formate Dehydrogenases; Formates; Hydrogen; Oxidation-Reduction; Phylogeny
PubMed: 30962355
DOI: 10.1128/JB.00160-19 -
Journal of Clinical Microbiology Jul 1991In 1985 the vernacular name Enteric Group 90 was coined for a small group of strains that had been referred to our laboratory as probable strains of Salmonella but did...
In 1985 the vernacular name Enteric Group 90 was coined for a small group of strains that had been referred to our laboratory as probable strains of Salmonella but did not agglutinate in Salmonella typing antisera. By DNA-DNA hybridization (hydroxyapatite method, 32P), seven strains of Enteric Group 90 were found to be closely related (98 to 100% at 60 degrees C and 94 to 100% at 75 degrees C) to the first strain received (0370-85). The relatedness of Enteric Group 90 to 62 strains of other species of the family Enterobacteriaceae was only 6 to 41%, with the highest values obtained with strains of Salmonella, Kluyvera, Shigella, Klebsiella, Enterobacter, and Citrobacter. We propose a new genus, Trabulsiella, with a single new species, Trabulsiella guamensis, for the highly related group of eight strains formerly known as Enteric Group 90. The type strain is designated ATCC 49490 (CDC 0370-85). T. guamensis strains grew well at 36 degrees C and had positive reactions in the following tests: methyl red, citrate utilization (Simmons) (38% positive at day 1, 88% positive at 2 days), H2S production, lysine decarboxylase, arginine dihydrolase (50% positive at 2 days, 100% positive at 7 days), ornithine decarboxylase, motility, growth in KCN medium, mucate fermentation, acetate utilization, nitrate reduction to nitrite, weak tyrosine hydrolysis (88% positive at 2 days, 100% positive at 7 days), and ONPG (o-nitrophenyl-beta-D-galactopyranoside) test. The strains fermented D-glucose with gas production and fermented L-arabinose, cellobiose, D-galactose, D-galacturonate, maltose, D-mannitol, D-mannose, L-rhamnose, D-sorbitol, trehalose, and D-xylose. T. guamensis strains had negative reactions in the following tests: indole production (13% positive), Voges-Proskauer, urea hydrolysis, phenylalanine deaminase, malonate utilization, lipase (corn oil), DNase, oxidase, pigment production, and acid production from adonitol, D-arabitol, dulcitol, erythritol, myo-inositol, melibiose, alpha-methyl-D-glucoside, raffinose, and sucrose. There were delayed positive reactions for gelatin liquefaction (22 degrees C), which was positive at 12 to 23 days, esculin hydrolysis (13% positive at day 1, 50% positive at 7 days), lactose fermentation (13% positive at 3 to 7 days, 100% positive at 8 to 10 days), glycerol fermentation (88% positive at 7 days), and salicin fermentation (13% positive at day 1, 88% positive at 7 days). All strains were susceptible by the disk diffusion method to colistin, nalidixic acid, gentamicin, streptomycin, kanamycin, chloramphenicol, and trimethoprim-sulfamethoxazole, and most strains were susceptible to sulfadiazine (75% susceptible), tetracycline (88%), and carbenicillin (75%). The strains were resistant to penicillin, cephalothin, and ampicillin. The strains were isolated from vacuum cleaner dust (five strains), soil (one strain), and human feces (two strains). Although T. guamensis can occur in human diarrheal stools, there is no evidence that it actually causes diarrhea. Its main interest to clinical microbiologists may be its possible misidentification as a strain Salmonella.
Topics: DNA, Bacterial; Drug Resistance, Microbial; Enterobacteriaceae; Humans; Nucleic Acid Hybridization; Salmonella; Sequence Homology, Nucleic Acid; Species Specificity; Terminology as Topic
PubMed: 1885744
DOI: 10.1128/jcm.29.7.1480-1485.1991 -
Current Research in Microbial Sciences 2022The topsoils and atmospheric dust aerosols of the various areas of the city of Moscow were studied. Most of the dust samples contained a considerable number of particles...
The topsoils and atmospheric dust aerosols of the various areas of the city of Moscow were studied. Most of the dust samples contained a considerable number of particles enriched in phosphorus - a sign of contamination by feces. A variety of species, including opportunistic and pathogenic species, were isolated from the topsoil and dust samples and identified using 16S rDNA nucleotide sequences: and . The greatest diversity of pathogens was isolated from spring soil and dust samples immediately after spring snowmelt. Antibiotic resistance of the isolated strains was tested using disks with a wide range of antimicrobial drugs: Amoxicillin, Ampicillin, Meropenem, Pefloxacin, Streptomycin, Ticarcillin+clavulanic acid, Fosfomycin, Ceftibuten, Ciprofloxacin. Resistance was observed in more than 22% of strains. The traffic area had a significant number of antibiotic-resistant strains, clearly indicating a high health risk from soil and dust exposure.
PubMed: 35909602
DOI: 10.1016/j.crmicr.2022.100124 -
Polish Journal of Microbiology 2015The presence of Gram-negative bacteria species, other than Salmonella spp., in the gallbladder of pigs was examined. Isolated Gram-negative bacteria were assigned to...
Isolation and Antimicrobial Testing of Aeromonas spp., Citrobacter spp., Cronobacter spp., Enterobacter spp., Escherichia spp., Klebsiella spp., and Trabulsiella spp. from the Gallbladder of Pigs.
The presence of Gram-negative bacteria species, other than Salmonella spp., in the gallbladder of pigs was examined. Isolated Gram-negative bacteria were assigned to species using the Microgen™ GnA+B-ID Systems. Of the 64 isolated strains 43 were identified as Escherichia coli, seven as Enterobacter spp., three each as Klebsiella spp., Citrobacterfreundii, Aeromonas hydrophila and Cronobacter sakazakii and one each as Escherichiafergusonii and Trabulsiella guamensis. Their antibiograms showed very high resistance to ampicillin, amoxicillin, tetracycline, chloramphenicol and sulfamethoxazole/trimethoprim. It was concluded that the pigs' gallbladder is a reservoir of potentially pathogenic Gram-negative bacteria for pork consumers.
Topics: Aeromonas; Animals; Anti-Bacterial Agents; Bacteria; Citrobacter; Drug Resistance, Bacterial; Enterobacter; Escherichia; Gallbladder; Klebsiella; Swine
PubMed: 26373181
DOI: No ID Found