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Proceedings of the National Academy of... Nov 1986This study assessed the possible relationship between methyl chloride (MeCl)-induced epididymal inflammation and the formation of dominant lethal mutations in sperm of...
This study assessed the possible relationship between methyl chloride (MeCl)-induced epididymal inflammation and the formation of dominant lethal mutations in sperm of Fischer 344 rats. Groups of 40 males were exposed to MeCl (3000 ppm 6 hr/day for 5 days), with or without concurrent treatment with the anti-inflammatory agent 3-amino-1-[m-(trifluoromethyl)phenyl]-2-pyrazoline (BW 755C; 10 mg/kg, i.p. 1 hr pre- and postexposure); BW 755C was shown previously to inhibit MeCl-induced epididymal inflammation. Control groups (n = 20) were either untreated, injected as described above with BW 755C, or injected on the afternoon of day 5 with triethylenemelamine (0.2 mg/kg), a known dominant lethal mutagen. Each male was caged with one female weekly for 3 weeks; 12-18 days after mating, females were killed to assess dominant lethal parameters. In females bred to MeCl-exposed males, significant increases were observed in postimplantation loss at postexposure week 1 (0.84 dead implants per female vs. 0.29 in untreated controls) and in dead implants/total implants at both week 1 (0.10 vs. 0.04 control) and week 2 (0.24 vs. 0.06 control). These increases were not observed in females bred to males treated with BW 755C during MeCl exposure. Coadministration of BW 755C to males along with MeCl also reduced the percentage of mated females with two or more postimplantation losses from 31% to 8% (week 1) and 30% to 12% (week 2). Therefore, the dominant lethal mutations induced by MeCl appear to be a consequence of its induction of inflammation in the epididymis. These data demonstrate the potential genotoxicity of inflammatory processes in vivo.
Topics: 4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine; Animals; DNA Damage; Epididymitis; Female; Male; Methyl Chloride; Mutation; Pyrazoles; Rats; Rats, Inbred F344; Spermatozoa
PubMed: 3095827
DOI: 10.1073/pnas.83.21.8087 -
The Ulster Medical Journal Dec 1963
Topics: Antimetabolites; Antineoplastic Agents; Carcinoma, Squamous Cell; Chemotherapy, Cancer, Regional Perfusion; Dactinomycin; Geriatrics; Head and Neck Neoplasms; Humans; Infusions, Parenteral; Melanoma; Melphalan; Methotrexate; Mouth Neoplasms; Neoplasms; Neoplasms, Muscle Tissue; Nitrogen Mustard Compounds; Sarcoma; Toxicology; Triethylenemelamine
PubMed: 14105942
DOI: No ID Found -
Genetics Apr 1982The relative induction of dominant-lethal mutations and heritable translocations in triethylenemelamine-treated postmeiotic germ cells of mice was determined depending...
Difference in the ratio of dominant-lethal mutations to heritable translocations produced in mouse spermatids and fully mature sperm after treatment with triethylenemelamine (TEM).
The relative induction of dominant-lethal mutations and heritable translocations in triethylenemelamine-treated postmeiotic germ cells of mice was determined depending on the stage treated. Males were mated either 11.5-14.5 days after treatment (middle spermatids) or less than 2.5 hours after treatment (fully mature sperm). Results clearly showed that, even through similar levels of dominant-lethal mutations were induced in fully mature sperm and in middle spermatids, the frequency of heritable translocations induced in mature sperm was markedly lower than that induced in middle spermatids. This observation was used, together with earlier ones, to suggest a mechanism by which dominant-lethal mutations and heritable translocations are produced following chemical treatment of male postmeiotic germ cels.
Topics: Animals; Female; Fertilization; Genes, Dominant; Genes, Lethal; Litter Size; Male; Mice; Mutation; Pregnancy; Spermatids; Spermatozoa; Translocation, Genetic; Triethylenemelamine
PubMed: 7117822
DOI: 10.1093/genetics/100.4.633 -
British Journal of Cancer Sep 1952
Topics: Animals; Humans; Mechlorethamine; Neoplasms; Neoplasms, Experimental; Nitrogen Mustard Compounds; Triethylenemelamine
PubMed: 12987553
DOI: 10.1038/bjc.1952.31 -
Blood Jul 1953
Topics: Animals; Aziridines; Leukemia; Piperazines; Rats; Sarcoma, Myeloid; Triethylenemelamine
PubMed: 13059051
DOI: No ID Found -
Clinical and Experimental Immunology Jan 1966One hundred patients with lymphoproliferative disease were reviewed. Seven of these patients were found to develop an autoimmune complication following the use of...
One hundred patients with lymphoproliferative disease were reviewed. Seven of these patients were found to develop an autoimmune complication following the use of radiation or radiomimetic drug. The literature suggesting that radiation can enhance as well as suppress the autoimmune response is summarized, and the possibility that therapy can trigger autoimmunity in these patients is emphasized.
Topics: Adult; Aged; Anemia, Hemolytic, Autoimmune; Chlorambucil; Humans; Leukemia, Lymphoid; Lymphoma, Non-Hodgkin; Male; Mechlorethamine; Middle Aged; Phosphorus Isotopes; Purpura, Thrombocytopenic; Radiation Injuries; Triethylenemelamine; Vascular Diseases
PubMed: 5953036
DOI: No ID Found -
Journal of Virology Mar 1974Fifty-four suppressible mutants of bacteriophage phi29 have been isolated with a variety of mutagens and assigned to eight complementation groups. Viral-specific protein...
Fifty-four suppressible mutants of bacteriophage phi29 have been isolated with a variety of mutagens and assigned to eight complementation groups. Viral-specific protein synthesis in UV light-irradiated, nonsuppressing Bacillus subtilis 60084 was analyzed with exponential acrylamide gels. Four additional phi29 proteins which were undetected on ordinary acrylamide gels are reported in this paper. Five phage phi29 proteins have been unambiguously assigned to specific cistrons. Two cistrons had pleiotropic effects on viral protein synthesis. Mutants in cistrons I or II were unable to synthesize DNA in nonsuppressing bacteria. Mutants in cistron I were unable to attach viral chromosomes to the host cell membrane, and the protein responsible for this function has been identified. The other viral protein playing a role in phage phi29 DNA synthesis is also identified and assigned to cistron II. Mutants in cistron II can attach viral chromosomes to membrane, but cannot synthesize DNA in nonsuppressing bacteria.
Topics: Alkanesulfonates; Bacillus subtilis; Bacteriophages; Bromodeoxyuridine; Cell Membrane; DNA, Viral; Electrophoresis, Polyacrylamide Gel; Genes; Genetic Complementation Test; Methods; Mutagens; Mutation; Nitrites; Radiation Effects; Recombination, Genetic; Thymidine; Triethylenemelamine; Tritium; Ultraviolet Rays; Viral Proteins
PubMed: 4362871
DOI: 10.1128/JVI.13.3.690-698.1974 -
British Journal of Cancer Apr 1980The cytotoxicity of hexamethylmelamine (HMM) and its metabolites pentamethylmelamine (PMM), N,2,2,4,6-tetramethylmelamine (TMM) and hydroxymethylpentamethylmelamine...
The cytotoxicity of hexamethylmelamine (HMM) and its metabolites pentamethylmelamine (PMM), N,2,2,4,6-tetramethylmelamine (TMM) and hydroxymethylpentamethylmelamine (HMPMM) and of the alkylating agent triethylenemelamine (TEM) were studied on a cell line derived from a human ovarian cancer, by measuring [3H]TdR uptake. After 24 h of incubation all the tested compounds inhibited [3H]TdR uptake, but only at a concentration of 100 micrograms/ml. However, after 120 h incubation, concentrations of 0.1--10 micrograms/ml resulted in highly significant cytotoxicity. HMPMM and TEM were the most active and their effect was not reversed 72 h after their removal. In our in vitro system no metabolism of HMM was observed.
Topics: Altretamine; Cell Line; Cells; Female; Humans; Ovarian Neoplasms; Thymidine; Time Factors; Triazines
PubMed: 6770884
DOI: 10.1038/bjc.1980.106 -
British Medical Journal Jan 1953
Topics: Administration, Oral; Hodgkin Disease; Neoplasms; Tablets, Enteric-Coated; Triethylenemelamine
PubMed: 12997844
DOI: 10.1136/bmj.1.4801.59 -
Journal of Bacteriology May 1959
Topics: Chlorides; Escherichia coli; Manganese; Mutagenesis; Mutation; Spheroplasts; Triethylenemelamine
PubMed: 13654227
DOI: 10.1128/jb.77.5.621-622.1959