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Journal of Food Protection Apr 2000A total of 160 meat product samples were collected from commercial outlets in Mexico City to investigate the presence of different species of Yersinia by the 4 degrees C...
A total of 160 meat product samples were collected from commercial outlets in Mexico City to investigate the presence of different species of Yersinia by the 4 degrees C enrichment method after 1, 3, 5, and 7 days of incubation using alkaline treatment and isolating in cefsulodin-Irgasan-novobiocin and MacConkey agars with Tween 80. Overall, Yersinia spp. were isolated from 27% of the samples analyzed, whereas 40% of the raw and only 13% of the precooked samples were contaminated. Although 2,970 colonies showed Yersinia characteristics, only 706 (24%) actually corresponded to this genus: 49% were Yersinia enterocolitica, 25% Yersinia kristensenii, 15% Yersinia intermedia, 9% Yersinia frederiksenii, and 2% Yersinia aldovae; 10% corresponded to biotype 2, 2% to biotype 3, and 4% to biotype 4. The presence of Yersinia in raw and cooked meat products represents a health risk for consumers in Mexico, where further clinical studies are needed to assess the epidemiological importance of this pathogen.
Topics: Animals; Chickens; Food Microbiology; Meat Products; Mexico; Swine; Yersinia
PubMed: 10772223
DOI: 10.4315/0362-028x-63.4.542 -
Biochimica Et Biophysica Acta.... Jan 2017Acr3 is a plasma membrane transporter, a member of the bile/arsenite/riboflavin transporter (BART) superfamily, which confers high-level resistance to arsenicals in the...
Acr3 is a plasma membrane transporter, a member of the bile/arsenite/riboflavin transporter (BART) superfamily, which confers high-level resistance to arsenicals in the yeast Saccharomyces cerevisiae. We have previously shown that the yeast Acr3 acts as a low affinity As(III)/H and Sb(III)/H antiporter. We have also identified several amino acid residues that are localized in putative transmembrane helices (TM) and appeared to be critical for the Acr3 activity. In the present study, the topology of Acr3 was investigated by insertion of glycosylation and factor Xa protease cleavage sites at predicted hydrophilic regions. The analysis of the glycosylation pattern and factor Xa cleavage products of resulting Acr3 fusion constructs provide evidence supporting a topological model of Acr3 with 10 TM segments and cytoplasmically oriented N- and C-terminal domains. Next, we investigated the role of the hydrophilic loop connecting TM8 and TM9, the large size of which is unique to members of the yeast Acr3 family of metalloid transporters. We found that a 28 amino acid deletion in this region does not affect Acr3 folding, trafficking substrate binding, or transport activity. Finally, we constructed a homology-based structural model of Acr3 using the crystal structure of the Yersinia frederiksenii homologue of the human bile acid sodium symporter ASBT.
Topics: Amino Acid Sequence; Arsenites; Binding Sites; Cell Membrane; Crystallography, X-Ray; Gene Expression; Glycosylation; Kinetics; Membrane Transport Proteins; Models, Molecular; Mutagenesis; Plasmids; Protein Binding; Protein Conformation, alpha-Helical; Protein Interaction Domains and Motifs; Protein Structure, Tertiary; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Sequence Alignment; Structural Homology, Protein; Substrate Specificity; beta-Fructofuranosidase
PubMed: 27836640
DOI: 10.1016/j.bbamem.2016.11.004 -
Microbiome Nov 2018The International Space Station (ISS) is an ideal test bed for studying the effects of microbial persistence and succession on a closed system during long space flight....
BACKGROUND
The International Space Station (ISS) is an ideal test bed for studying the effects of microbial persistence and succession on a closed system during long space flight. Culture-based analyses, targeted gene-based amplicon sequencing (bacteriome, mycobiome, and resistome), and shotgun metagenomics approaches have previously been performed on ISS environmental sample sets using whole genome amplification (WGA). However, this is the first study reporting on the metagenomes sampled from ISS environmental surfaces without the use of WGA. Metagenome sequences generated from eight defined ISS environmental locations in three consecutive flights were analyzed to assess the succession and persistence of microbial communities, their antimicrobial resistance (AMR) profiles, and virulence properties. Metagenomic sequences were produced from the samples treated with propidium monoazide (PMA) to measure intact microorganisms.
RESULTS
The intact microbial communities detected in Flight 1 and Flight 2 samples were significantly more similar to each other than to Flight 3 samples. Among 318 microbial species detected, 46 species constituting 18 genera were common in all flight samples. Risk group or biosafety level 2 microorganisms that persisted among all three flights were Acinetobacter baumannii, Haemophilus influenzae, Klebsiella pneumoniae, Salmonella enterica, Shigella sonnei, Staphylococcus aureus, Yersinia frederiksenii, and Aspergillus lentulus. Even though Rhodotorula and Pantoea dominated the ISS microbiome, Pantoea exhibited succession and persistence. K. pneumoniae persisted in one location (US Node 1) of all three flights and might have spread to six out of the eight locations sampled on Flight 3. The AMR signatures associated with β-lactam, cationic antimicrobial peptide, and vancomycin were detected. Prominent virulence factors were cobalt-zinc-cadmium resistance and multidrug-resistance efflux pumps.
CONCLUSIONS
There was an increase in AMR and virulence gene factors detected over the period sampled, and metagenome sequences of human pathogens persisted over time. Comparative analysis of the microbial compositions of ISS with Earth analogs revealed that the ISS environmental surfaces were different in microbial composition. Metagenomics coupled with PMA treatment would help future space missions to estimate problematic risk group microbial pathogens. Cataloging AMR/virulence characteristics, succession, accumulation, and persistence of microorganisms would facilitate the development of suitable countermeasures to reduce their presence in the closed built environment.
Topics: Archaea; Azides; Bacteria; Drug Resistance, Bacterial; Fungi; Humans; Metagenome; Microbiota; Propidium; Space Flight; Spacecraft; Virulence
PubMed: 30424821
DOI: 10.1186/s40168-018-0585-2 -
Journal of Food Protection Jul 1987Thirty-seven (16.9%) of 219 raw milk samples and 38 (13.7%) of 280 pasteurized milk samples were positive for Yersinia sp. The isolates from raw milk samples include...
Thirty-seven (16.9%) of 219 raw milk samples and 38 (13.7%) of 280 pasteurized milk samples were positive for Yersinia sp. The isolates from raw milk samples include Yersinia enterocolitica (32.4%) comprising biotype 1 (0:5, 10.8%), and biotype 2 (0:10 K1, 1.6%); Yersinia intermedia (64.9%) comprising 0:18 (40.5%), 0:7,8 (8.1%), 0:16 (2,7%) and non-typable (13.5%) and Yersinia frederiksenii (0:22, 2.7%). The isolates from pasteurized milk samples include Y. enterocolitica (41.5%) comprising 0:5 (31.7%), 0:13 (2.4%), 0:7,8 (2.4%) and 0:16 (4.8%); Y. frederiksenii (56.1%) comprising 0:27 (7.3%), 0:25,35 (12.2%), non-typable (36.6%) and Y. intermedia (non-typable, 2.4%). Most Y. enterocolitica and about one third of non- Y. enterocolitica strains produce heat-stable toxin (ST). Antibiotic susceptibility, autoagglutination capacity and calcium-dependency of strains also were investigated.
PubMed: 30965482
DOI: 10.4315/0362-028X-50.7.580 -
Journal of Pathogens 2011Retron is a retroelement that encodes msDNA (multicopy single-stranded DNA) which was significantly found mainly in Gram-negative pathogenic bacteria. We screened...
A Novel msDNA (Multicopy Single-Stranded DNA) Strain Present in Yersinia frederiksenii ATCC 33641 Contig01029 Enteropathogenic Bacteria with the Genomic Analysis of It's Retron.
Retron is a retroelement that encodes msDNA (multicopy single-stranded DNA) which was significantly found mainly in Gram-negative pathogenic bacteria. We screened Yersinia frederiksenii ATCC 33641 contig01029 for the presence of retroelement by using bioinformatics tools and characterized a novel retron-Yf79 on the chromosome that encodes msDNA-Yf79. In this study, we perceived that, the codon usage of retron-Yf79 were noteworthy different from those of the Y. frederiksenii genome. It demonstrates that, the retron-Yf79 was a foreign DNA element and integrated into this organism genome during their evolution. In addition to this, we have observed a transposase gene which is located just downstream of retron-Yf79. So, the enzyme might be responsible for the transposition of this novel retron element.
PubMed: 22567337
DOI: 10.4061/2011/693769 -
Journal of Clinical Microbiology Nov 1990A total of 1,835 Yersinia spp. were isolated from 925 (60.5%) of 1,530 wild mice and from 139 (79.9%) of 174 moles living in mountainous areas of eastern Shimane...
A total of 1,835 Yersinia spp. were isolated from 925 (60.5%) of 1,530 wild mice and from 139 (79.9%) of 174 moles living in mountainous areas of eastern Shimane Prefecture, Japan. The Yersinia spp. included 1,106 Yersinia enterocolitica, 26 Y. enterocolitica-like, 176 Yersinia mollaretii, 149 Yersinia frederiksenii, 70 Yersinia intermedia, 231 Yersinia kristensenii, 5 Yersinia aldovae, and 72 Yersinia pseudotuberculosis. Human pathogenic Y. enterocolitica was not isolated. Y. pseudotuberculosis was divided into 10 virulent 40- to 50-MDa plasmid-positive (P+) strains (serotypes 1b, 4b, and untypeable) and 62 plasmid-negative (P-) strains (serotypes 1b, 2b, 2c, 4a, 5a, 5b, 6, 7, and untypeable). P+ strains of serotypes 1b (two strains), 4b (seven strains), and untypeable (one strain) were isolated from nine Apodemus specious and one Apodemus argenteus. The isolates of Yersinia spp. were more frequently detected in newborn mice and during the breeding season. The P+ Y. pseudotuberculosis strains were recovered at less than 10(4) cells per g of the cecal contents. Thus, the prevalence of Yersinia spp. in small wild animals depends on the newborn animals born during the cold months, and wild mice in mountainous areas are important reservoirs of Y. pseudotuberculosis.
Topics: Animals; Female; Japan; Male; Moles; Muridae; Seasons; Virulence; Yersinia pseudotuberculosis; Yersinia pseudotuberculosis Infections
PubMed: 2254420
DOI: 10.1128/jcm.28.11.2448-2455.1990 -
Nature Jan 2014Bile acids are synthesized from cholesterol in hepatocytes and secreted through the biliary tract into the small intestine, where they aid in absorption of lipids and...
Bile acids are synthesized from cholesterol in hepatocytes and secreted through the biliary tract into the small intestine, where they aid in absorption of lipids and fat-soluble vitamins. Through a process known as enterohepatic recirculation, more than 90% of secreted bile acids are then retrieved from the intestine and returned to the liver for resecretion. In humans, there are two Na(+)-dependent bile acid transporters involved in enterohepatic recirculation, the Na(+)-taurocholate co-transporting polypeptide (NTCP; also known as SLC10A1) expressed in hepatocytes, and the apical sodium-dependent bile acid transporter (ASBT; also known as SLC10A2) expressed on enterocytes in the terminal ileum. In recent years, ASBT has attracted much interest as a potential drug target for treatment of hypercholesterolaemia, because inhibition of ASBT reduces reabsorption of bile acids, thus increasing bile acid synthesis and consequently cholesterol consumption. However, a lack of three-dimensional structures of bile acid transporters hampers our ability to understand the molecular mechanisms of substrate selectivity and transport, and to interpret the wealth of existing functional data. The crystal structure of an ASBT homologue from Neisseria meningitidis (ASBT(NM)) in detergent was reported recently, showing the protein in an inward-open conformation bound to two Na(+) and a taurocholic acid. However, the structural changes that bring bile acid and Na(+) across the membrane are difficult to infer from a single structure. To understand the structural changes associated with the coupled transport of Na(+) and bile acids, here we solved two structures of an ASBT homologue from Yersinia frederiksenii (ASBTYf) in a lipid environment, which reveal that a large rigid-body rotation of a substrate-binding domain gives the conserved 'crossover' region, where two discontinuous helices cross each other, alternating accessibility from either side of the cell membrane. This result has implications for the location and orientation of the bile acid during transport, as well as for the translocation pathway for Na(+).
Topics: Bacterial Proteins; Bile Acids and Salts; Biological Transport; Carrier Proteins; Cell Membrane; Crystallography, X-Ray; Membrane Glycoproteins; Models, Molecular; Protein Conformation; Reproducibility of Results; Rotation; Sodium; Structure-Activity Relationship; Yersinia
PubMed: 24317697
DOI: 10.1038/nature12811 -
Journal of Clinical Microbiology Jan 1994Yersinia enterocolitica strains of serotypes lethal to mice have been reported previously to produce an endogenous siderophore. In this study, an ethyl... (Comparative Study)
Comparative Study
Yersinia enterocolitica strains of serotypes lethal to mice have been reported previously to produce an endogenous siderophore. In this study, an ethyl acetate-extractable siderophore was characterized and given the name yersiniophore. Yersiniophore was produced by 16 of 16 human isolates of serotypes O:4, O:4,32, O:8, O:21, and one nonhuman isolate of serotype O:21. It was not produced by isolates of serotype O:3, O:5, or O:9. One strain of Yersinia pseudotuberculosis produced yersiniophore, but strains of Yersinia kristensenii, Yersinia frederiksenii, and Yersinia intermedia did not produce or utilize yersiniophore. Food and water isolates of Y. enterocolitica produced a water-soluble siderophore but not yersiniophore. Sixty-two strains of Y. enterocolitica including 42 isolates from human infections, 2 animal isolates, and 18 water and food isolates were examined for utilization of yersiniophore, the water-soluble siderophore, and ferrioxamine. Yersiniophore promoted growth rate, iron binding, and uptake in 17 of 62 strains, all of which produced yersiniophore. Ten of 17 food and water isolates and one human isolate were capable of utilizing the water-soluble siderophore. Utilization studies suggest that at least one additional water-soluble siderophore may be produced. Ferrioxamine promoted the growth of 60 of 62 strains examined; however, only the 17 strains which produced yersiniophore actively accumulated [59Fe]ferrioxamine. Yersiniophore production and utilization may be important in clinical infections since all human strains belonging to serotype O:8 produced yersinophore. The water-soluble siderophore was not detected in human isolates.
Topics: Animals; Bacterial Outer Membrane Proteins; Deferoxamine; Environmental Microbiology; Ferric Compounds; Gene Expression Regulation, Bacterial; Humans; Iron; Siderophores; Species Specificity; Yersinia; Yersinia Infections; Yersinia enterocolitica
PubMed: 8126201
DOI: 10.1128/jcm.32.1.32-39.1994 -
Journal of Clinical Microbiology May 1984A total of 93 Yersinia spp. were isolated from 68 of 252 dogs in Shimane Prefecture, Japan. The Yersinia spp. were 70 Yersinia enterocolitica isolates from 50 dogs, 2...
A total of 93 Yersinia spp. were isolated from 68 of 252 dogs in Shimane Prefecture, Japan. The Yersinia spp. were 70 Yersinia enterocolitica isolates from 50 dogs, 2 Yersinia frederiksenii isolates from 2 dogs, 5 Yersinia intermedia isolates from 5 dogs, and 16 Yersinia pseudotuberculosis isolates from 16 dogs. Fifteen of 70 Y. enterocolitica isolates belonged to biotype 4 serotype O3 phagotype 8 (6 isolates), biotype 4 (maltose negative) serotype O3 phagotype 8 (4 isolates), biotype 3 (Voges-Proskauer and sorbose negative) serotype O3 phagotype 9 (2 isolates), and biotype 2 serotype O5.27 (3 isolates). The other 55 Y. enterocolitica isolates belonged to biotype 1 and were classified into serotypes O4, O5, O7 .8, O9, O10 , O12 , O13 .7, O14 , O15 , and untypable. Sixteen Y. pseudotuberculosis isolates belonged to serotypes IB (two), IIB (two), IVA (two), IVB (three), VA (five), and untypable (two). These isolates were mostly detected in cold months. Y. enterocolitica serotype O3 and Y. pseudotuberculosis were isolated more frequently from puppies. Y. enterocolitica serotype O3 was recovered at less than 10(7.0) cells per g of the jejunal-to-rectal contents and at less than 10(2) cells per g of mesenteric lymph nodes. Y. pseudotuberculosis was recovered at less than 10(4.3) cells per g of the cecal-to-rectal contents and at less than 10(2.9) cells per g of mesenteric lymph nodes. Almost all strains of Y. enterocolitica biotype 1, Y. intermedia, and Y. frederiksenii were recovered at less than 10(2) cells per g of gastric-to-rectal contents.
Topics: Age Factors; Animals; Dogs; Housing, Animal; Prospective Studies; Seasons; Serotyping; Time Factors; Yersinia; Yersinia enterocolitica
PubMed: 6736224
DOI: 10.1128/jcm.19.5.616-622.1984 -
Nucleic Acids Research Jan 2001The rpoH regulatory region of different members of the enteric bacteria family was sequenced or downloaded from GenBank and compared. In addition, the transcriptional... (Comparative Study)
Comparative Study
The rpoH regulatory region of different members of the enteric bacteria family was sequenced or downloaded from GenBank and compared. In addition, the transcriptional start sites of rpoH of Yersinia frederiksenii and Proteus mirabilis, two distant members of this family, were determined. Sequences similar to the sigma(70) promoters P1, P4 and P5, to the sigma(E) promoter P3 and to boxes DnaA1, DnaA2, cAMP receptor protein (CRP) boxes CRP1, CRP2 and box CytR present in Escherichia coli K12, were identified in sequences of closely related bacteria such as: E.coli, Shigella flexneri, Salmonella enterica serovar Typhimurium, Citrobacter freundii, Enterobacter cloacae and Klebsiella pneumoniae. In more distant bacteria, Y.frederiksenii and P.mirabilis, the rpoH regulatory region has a distal P1-like sigma(70) promoter and two proximal promoters: a heat-induced sigma(E)-like promoter and a sigma(70) promoter. Sequences similar to the regulatory boxes were not identified in these bacteria. This study suggests that the general pattern of transcription of the rpoH gene in enteric bacteria includes a distal sigma(70) promoter, >200 nt upstream of the initiation codon, and two proximal promoters: a heat-induced sigma(E)-like promoter and a sigma(70) promoter. A second proximal sigma(70) promoter under catabolite-regulation is probably present only in bacteria closely related to E.coli.
Topics: Base Composition; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial; Conserved Sequence; Enterobacteriaceae; Genes, Bacterial; Heat-Shock Proteins; Molecular Sequence Data; Phylogeny; Promoter Regions, Genetic; Proteus mirabilis; Regulatory Sequences, Nucleic Acid; Sequence Homology, Nucleic Acid; Sigma Factor; Transcription Factors; Transcription, Genetic; Yersinia
PubMed: 11139607
DOI: 10.1093/nar/29.2.380