-
International Journal of Molecular... Apr 2023The adhesion G-protein-coupled receptor is a seven-transmembrane receptor protein with a complex structure. Impaired has been found to cause developmental damage to the...
The adhesion G-protein-coupled receptor is a seven-transmembrane receptor protein with a complex structure. Impaired has been found to cause developmental damage to the human brain, resulting in intellectual disability and motor dysfunction. To date, studies on deficiency in zebrafish have been limited to the nervous system, and there have been no reports of its systemic effects on juvenile fish at developmental stages. In order to explore the function of in zebrafish, the CRISPR/Cas9 gene-editing system was used to construct a -knockout zebrafish. Subsequently, the differentially expressed genes (DEGs) at the transcriptional level between the 3 days post fertilization (dpf) homozygotes of the mutation and the wildtype zebrafish were analyzed via RNA-seq. The results of the clustering analysis, quantitative PCR (qPCR), and in situ hybridization demonstrated that the expression of innate immunity-related genes in the mutant was disordered, and multiple genes encoding digestive enzymes of the pancreatic exocrine glands were significantly downregulated in the mutant. Motor ability tests demonstrated that the zebrafish were more active, and this change was more pronounced in the presence of cold and additional stimuli. In conclusion, our results revealed the effect of deletion on the gene expression of juvenile zebrafish and found that the mutant was extremely active, providing an important clue for studying the mechanism of in the development of juvenile zebrafish.
Topics: Animals; Humans; Mutation; Receptors, G-Protein-Coupled; Transcriptome; Zebrafish; Zebrafish Proteins
PubMed: 37175447
DOI: 10.3390/ijms24097740 -
The International Journal of... 2010Gastrulation involves of a series of coordinated cell movements to organize the germ layers and establish the major body axes of the embryo. One gastrulation movement is... (Review)
Review
Gastrulation involves of a series of coordinated cell movements to organize the germ layers and establish the major body axes of the embryo. One gastrulation movement is epiboly, which involves the thinning and spreading of a multilayered cell sheet. Epiboly plays a prominent role in zebrafish gastrulation and studies of zebrafish epiboly have provided insights into basic cellular properties and mechanisms of morphogenesis that are widely used in animal development. Although considerable progress has been made in identifying molecules that are required for epiboly, we still understand very little about how these factors cooperate to drive the process. Here, we review work on the molecular and cellular basis of zebrafish epiboly in order to identify unifying themes and to highlight some of the current open questions.
Topics: Animals; Gastrula; Gastrulation; Gene Expression Regulation, Developmental; Microtubules; Signal Transduction; Zebrafish; Zebrafish Proteins
PubMed: 20712002
DOI: 10.1387/ijdb.093028sl -
Developmental Biology Jan 2020
Topics: Animals; Biomedical Research; Developmental Biology; Disease Models, Animal; Poland; Zebrafish
PubMed: 31705847
DOI: 10.1016/j.ydbio.2019.11.003 -
Trends in Cell Biology Jan 2021Dissemination and replication of viruses into hosts is a multistep process where viral particles infect, navigate, and indoctrinate various cell types. Viruses can reach... (Review)
Review
Dissemination and replication of viruses into hosts is a multistep process where viral particles infect, navigate, and indoctrinate various cell types. Viruses can reach tissues that are distant from their infection site by subverting subcellular mechanisms in ways that are, sometimes, disruptive. Modeling these steps, at appropriate resolution and within animal models, is cumbersome. Yet, mimicking these strategies in vitro fails to recapitulate the complexity of the cellular ecosystem. Here, we will discuss relevant in vivo platforms to dissect the cellular and molecular programs governing viral dissemination and briefly discuss organoid and ex vivo alternatives. We will focus on the zebrafish model and will describe how it provides a transparent window to unravel new cellular mechanisms of viral dissemination in vivo.
Topics: Animals; Embryo, Nonmammalian; Humans; Mice; Models, Animal; Viruses; Zebrafish
PubMed: 33023793
DOI: 10.1016/j.tcb.2020.09.005 -
Journal of Vascular Research 2019Cardiovascular diseases such as coronary heart disease, myocardial infarction, and cardiac arrhythmia are the leading causes of morbidity and mortality in developed... (Review)
Review
Cardiovascular diseases such as coronary heart disease, myocardial infarction, and cardiac arrhythmia are the leading causes of morbidity and mortality in developed countries and are steadily increasing in developing countries. Fundamental mechanistic studies at the molecular, cellular, and animal model levels are critical for the diagnosis and treatment of these diseases. Despite being phylogenetically distant from humans, zebrafish share remarkable similarity in the genetics and electrophysiology of the cardiovascular system. In the last 2 decades, the development and deployment of innovative genetic manipulation techniques greatly facilitated the application of zebrafish as an animal model for studying basic biology and diseases. Hemodynamic shear stress is intimately involved in vascular development and homeostasis. The critical mechanosensitive signaling pathways in cardiovascular development and pathophysiology previously studied in mammals have been recapitulated in zebrafish. In this short article, we reviewed recent knowledge about the role of mechanosensitive pathways such as Notch, PKCε/PFKFB3, and Wnt/Ang2 in cardiovas-cular development and homeostasis from studies in the -zebrafish model.
Topics: Animals; Cardiovascular System; Gene Expression Regulation, Developmental; Hemodynamics; Homeostasis; Mechanotransduction, Cellular; Organogenesis; Stress, Mechanical; Zebrafish; Zebrafish Proteins
PubMed: 31466069
DOI: 10.1159/000501883 -
Comprehensive Physiology Jul 2013The liver performs a large number of essential synthetic and regulatory functions that are acquired during fetal development and persist throughout life. Their... (Review)
Review
The liver performs a large number of essential synthetic and regulatory functions that are acquired during fetal development and persist throughout life. Their disruption underlies a diverse group of heritable and acquired diseases that affect both pediatric and adult patients. Although experimental analyses used to study liver development and disease are typically performed in cell culture models or rodents, the zebrafish is increasingly used to complement discoveries made in these systems. Forward and reverse genetic analyses over the past two decades have shown that the molecular program for liver development is largely conserved between zebrafish and mammals, and that the zebrafish can be used to model heritable human liver disorders. Recent work has demonstrated that zebrafish can also be used to study the mechanistic basis of acquired liver diseases. Here, we provide a comprehensive summary of how the zebrafish has contributed to our understanding of human liver development and disease.
Topics: Animals; Disease Models, Animal; Humans; Liver; Liver Diseases; Signal Transduction; Species Specificity; Transcription, Genetic; Zebrafish
PubMed: 23897685
DOI: 10.1002/cphy.c120021 -
Developmental Dynamics : An Official... Nov 2017The Zebrafish has emerged to become a powerful vertebrate animal model for cardiovascular research in recent years. Its advantages include easy genetic manipulation,... (Review)
Review
The Zebrafish has emerged to become a powerful vertebrate animal model for cardiovascular research in recent years. Its advantages include easy genetic manipulation, transparency, small size, low cost, and the ability to survive without active circulation at early stages of development. Sequencing the whole genome and identifying ortholog genes with human genome made it possible to induce clinically relevant cardiovascular defects via genetic approaches. Heart function and disturbed hemodynamics need to be assessed in a reliable manner for these disease models in order to reveal the mechanobiology of induced defects. This effort requires precise determination of blood flow patterns as well as hemodynamic stress (i.e., wall shear stress and pressure) levels within the developing heart. While traditional approach involves time-lapse brightfield microscopy to track cell and tissue movements, in more recent studies fast light-sheet fluorescent microscopes are utilized for that purpose. Integration of more complicated techniques like particle image velocimetry and computational fluid dynamics modeling for hemodynamic analysis holds a great promise to the advancement of the Zebrafish studies. Here, we discuss the latest developments in heart function and hemodynamic analysis for Zebrafish embryos and conclude with our future perspective on dynamic analysis of the Zebrafish cardiovascular system. Developmental Dynamics 246:868-880, 2017. © 2017 Wiley Periodicals, Inc.
Topics: Animals; Blood Flow Velocity; Embryo, Nonmammalian; Heart; Hemodynamics; Stress, Mechanical; Zebrafish
PubMed: 28249360
DOI: 10.1002/dvdy.24497 -
BMC Biology Dec 2015Zebrafish are able to regenerate various organs and tissues after damage or amputation. To understand better the genetic controls of this process, the authors of this...
Zebrafish are able to regenerate various organs and tissues after damage or amputation. To understand better the genetic controls of this process, the authors of this study investigated the expression of two genes previously implicated in fin regeneration using semi-quantitative RT-PCR, at three time points after fin amputation (T1, T2, and T3 in Fig. 1, corresponding to the initiation, middle, and end of fin regeneration, respectively). Briefly, the RT-PCR procedure involved isolating messenger RNA (mRNA) from a matched amount of zebrafish cells from the site of fin regeneration at the three time points, and using primers specific to each gene to selectively detect mRNA as an indicator of gene expression levels. The authors used total genomic DNA isolated from zebrafish cells as a positive control, and no RNA or DNA template as a negative control. They found that Gene 1 was only expressed early on in the process, while Gene 2 expression gradually increased during fin regeneration, reaching a peak of expression toward the end of the process. This provides some detailed information that could be useful in elucidating the function of these genes in fin regeneration.
Topics: Amputation, Surgical; Animal Fins; Animals; Gene Expression Regulation; RNA, Messenger; Regeneration; Reverse Transcriptase Polymerase Chain Reaction; Zebrafish
PubMed: 26694721
DOI: 10.1186/s12915-015-0217-2 -
Methods in Cell Biology 2017Zebrafish chemical screening allows for an in vivo assessment of small molecule modulation of biological processes. Compound toxicities, chemical alterations by... (Review)
Review
Zebrafish chemical screening allows for an in vivo assessment of small molecule modulation of biological processes. Compound toxicities, chemical alterations by metabolism, pharmacokinetic and pharmacodynamic properties, and modulation of cell niches can be studied with this method. Furthermore, zebrafish screening is straightforward and cost effective. Zebrafish provide an invaluable platform for novel therapeutic discovery through chemical screening.
Topics: Animals; Drug Discovery; Drug Evaluation, Preclinical; Embryo, Nonmammalian; High-Throughput Screening Assays; Humans; Small Molecule Libraries; Zebrafish
PubMed: 28129862
DOI: 10.1016/bs.mcb.2016.10.004 -
Translational Research : the Journal of... Feb 2014Over the past several decades, the zebrafish has become one of the major vertebrate model organisms used in biomedical research. In this arena, the zebrafish has emerged... (Review)
Review
Over the past several decades, the zebrafish has become one of the major vertebrate model organisms used in biomedical research. In this arena, the zebrafish has emerged as an applicable system for the study of kidney diseases and renal regeneration. The relevance of the zebrafish model for nephrology research has been increasingly appreciated as the understanding of zebrafish kidney structure, ontogeny, and the response to damage has steadily expanded. Recent studies have documented the amazing regenerative characteristics of the zebrafish kidney, which include the ability to replace epithelial populations after acute injury and to grow new renal functional units, termed nephrons. Here we discuss how nephron composition is conserved between zebrafish and mammals, and highlight how recent findings from zebrafish studies utilizing transgenic technologies and chemical genetics can complement traditional murine approaches in the effort to dissect how the kidney responds to acute damage and identify therapeutics that enhance human renal regeneration.
Topics: Animals; Kidney; Models, Animal; Regeneration; Zebrafish
PubMed: 24183931
DOI: 10.1016/j.trsl.2013.10.003