Laboratory Procedure
affinity chromatography
[ uh-fin-i-tee kroh-muh-tog-ruh-fee ]
Subclass of:
Liquid Chromatography
Definitions related to chromatography, affinity:
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(affinity chromatography) A chromatographic separation technique based on the ability of proteins to bind specific ligands reversibly.NCI ThesaurusU.S. National Cancer Institute, 2021
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(affinity chromatography) Specific ligands are covalently attached to the stationary phase, usually gel beads, to retain a compound of interest by binding; the compound is then eluted with free ligand or solvents which disrupt binding.CRISP ThesaurusNational Institutes of Health, 2006
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A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)NLM Medical Subject HeadingsU.S. National Library of Medicine, 2021
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