Laboratory Procedure
affinity chromatography
[ uh-fin-i-tee kroh-muh-tog-ruh-fee ]
Subclass of:
Liquid Chromatography
Definitions related to chromatography, affinity:
  • (affinity chromatography) A chromatographic separation technique based on the ability of proteins to bind specific ligands reversibly.
    NCI
    U.S. National Cancer Institute, 2021
  • (affinity chromatography) Specific ligands are covalently attached to the stationary phase, usually gel beads, to retain a compound of interest by binding; the compound is then eluted with free ligand or solvents which disrupt binding.
    CRISP Thesaurus
    National Institutes of Health, 2006
  • A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
    NLM Medical Subject Headings
    U.S. National Library of Medicine, 2025
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