Molecular Biology Research Technique
DNA footprinting
Subclass of:
Genetic Techniques
Definitions related to dna footprinting:
  • A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
    NLM Medical Subject Headings
    U.S. National Library of Medicine, 2021
  • A procedure that determines the sequence specificity for DNA binding proteins. Target DNA is end-labeled and exposed to the protein of interest. The sample is then treated with a DNA damaging agent that cleaves at random base pairs. Protein-bound DNA is protected from the cleavage and can be analyzed by gel electrophoresis.
    NCI Thesaurus
    U.S. National Cancer Institute, 2021
  • Test for the presence and specificity of DNA binding proteins and their binding sites on DNA, based on their tendency to prevent DNA cleavage by restriction enzymes.
    CRISP Thesaurus
    National Institutes of Health, 2006
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