Laboratory Procedure
southern blotting Audio
South·ern blot
Subclass of:
Molecular Probe Techniques; Electrophoresis
Definitions related to southern blot assay (procedure):
  • (southern blot) Electrophoresis-based technique used in genetic testing to detect large deletions in DNA that can be missed by PCR-based genetic testing methods.
    NCI Dictionary of Cancer Terms
    U.S. National Cancer Institute, 2021
  • (southern blotting) A technique for the detection of specific DNA fragments that have been separated by polyacrylamide-gel electrophoresis, transferred to a nitrocellulose or other type of paper or nylon membrane, and detected by hybridization and visualization with a labeled probe.
    NCI Thesaurus
    U.S. National Cancer Institute, 2021
  • (southern blotting) Method of identifying DNA by cDNA or RNA hybridization after electrophoresis and transfer to nitrocellulose or other fixative.
    CRISP Thesaurus
    National Institutes of Health, 2006
  • (blotting, southern) A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
    NLM Medical Subject Headings
    U.S. National Library of Medicine, 2021
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