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Indian Journal of Pharmacology 2021
Topics: Anti-Ulcer Agents; Gastroesophageal Reflux; Humans; India; Legislation, Medical; Practice Patterns, Physicians'; Ranitidine
PubMed: 33976005
DOI: 10.4103/ijp.ijp_929_20 -
Biomolecules Aug 2021Different species belonging to the genus (Acyonaceae) are a rich resource for bioactive secondary metabolites. The literature reveals that the gastroprotective effects...
Different species belonging to the genus (Acyonaceae) are a rich resource for bioactive secondary metabolites. The literature reveals that the gastroprotective effects of marine secondary metabolites have not been comprehensively studied in vivo. Hence, the present investigation aimed to examine and determine the anti-ulcer activity of 4,24-dimethyl-5-cholest-8,18-dihydroxy,22-en-3-ol () isolated from samples of a species. This in vivo study was supported by in silico molecular docking and protein-protein interaction techniques. Oral administration of reduced rat stomach ulcers with a concurrent increase in gastric mucosa. Molecular docking calculations against the H/K-ATPase transporter showed a higher binding affinity of , with a docking score value of -9.9 kcal/mol and a p value of 59.7 nM, compared to ranitidine (a commercial proton pump inhibitor, which gave values of -6.2 kcal/mol and 27.9 µM, respectively). The combined PEA-reactome analysis results revealed promising evidence of potency as an anti-ulcer compound through significant modulation of the gene set controlling the PI3K signaling pathway, which subsequently plays a crucial role in signaling regarding epithelialization and tissue regeneration, tissue repairing and tissue remodeling. These results indicate a probable protective role for against ethanol-induced gastric ulcers.
Topics: Animals; Anthozoa; Anti-Ulcer Agents; Computer Simulation; Ethanol; Female; Gastric Mucosa; Glycoproteins; Inflammation; Magnetic Resonance Spectroscopy; Molecular Docking Simulation; Phosphatidylinositol 3-Kinases; Protein Interaction Mapping; Rats; Rats, Wistar; Signal Transduction; Sterols; Stomach Ulcer; Ulcer
PubMed: 34439913
DOI: 10.3390/biom11081247 -
BMJ Case Reports Sep 2019
Topics: Aged; Anti-Ulcer Agents; Drug Substitution; Gastroesophageal Reflux; Humans; Male; Omeprazole; Polyps; Ranitidine; Stomach Diseases
PubMed: 31511271
DOI: 10.1136/bcr-2019-231341 -
Journal of Ethnopharmacology Apr 2021The incidence of gastric mucosa lesions in the adult population has increased mainly due to the continued use of nonsteroidal anti-inflammatory drugs (NSAIDs). The...
ETHNOPHARMACOLOGICAL RELEVANCE
The incidence of gastric mucosa lesions in the adult population has increased mainly due to the continued use of nonsteroidal anti-inflammatory drugs (NSAIDs). The cashew (Anacardium occidentale L.) is a tropical tree, cultivated in several countries, whose barks, leaves and pseudofruit (cashew apple) are popularly used in traditional medicine for the treatment of many diseases, including gastric ulcer.
AIM
Our study evaluated the potential gastroprotective effect of the carotenoid and anacardic acids-enriched aqueous extract (CAE), prepared from cashew apple pomace, in the dose-repeated acetylsalicylic acid (ASA)-induced gastric lesions model in rats.
MATERIAL AND METHODS
After randomly distribution into five group (G1 - G5, n = 8 animals/group), male Wistar rats were daily treated with ASA solution (200 mg/kg, 5 ml/kg, G2 - G5) or potable water (Satellite group, G1) during 14 days. From 8 to 14 experimental day, rats in G3 - G5 groups were orally treated with CAE (50, 100 and 500 mg/kg, 5 ml/kg, respectively). Body weight was measured on 0, 7 and 14 day. On the 14 experimental day, all surviving animals were euthanized for macroscopic evaluation of the inner organs and stomach removal. After weighting, each stomach was properly prepared for biochemical analysis [myeloperoxidase activity (MPO), reduced glutathione analysis (GSH), IL-1β, CXCL2/MIP-2, TNF-α and IL-10 levels].
RESULTS
At the most efficient dose (100 mg/kg, p.o.), CAE-treated animals showed a slight improvement in the macroscopic aspect of gastric mucosa associated with significant (p < 0.05) reduced levels of IL-1β, CXCL2/MIP-2, and MPO activity besides increased levels of GSH (partially), and IL-10 in stomach tissues.
CONCLUSIONS
The present study demonstrated that the carotenoid and anacardic acids-enriched extract obtained from cashew apple pomace is a promising raw material for the development of herbal medicine and/or functional food supplements for the adjuvant treatment of NSAIDs-induced gastric ulcers.
Topics: Anacardic Acids; Anacardium; Animals; Anti-Inflammatory Agents, Non-Steroidal; Anti-Ulcer Agents; Aspirin; Carotenoids; Chemokine CXCL2; Disease Models, Animal; Gastric Mucosa; Glutathione; Interleukin-10; Interleukin-1beta; Male; Peroxidase; Plant Extracts; Protective Agents; Rats, Wistar; Stomach Ulcer; Rats
PubMed: 33359862
DOI: 10.1016/j.jep.2020.113744 -
Scientific Reports May 2023Breast cancer and diabetes are significant health challenges, and effective treatments for both diseases are lacking. Proton pump inhibitors (PPIs) have demonstrated...
Breast cancer and diabetes are significant health challenges, and effective treatments for both diseases are lacking. Proton pump inhibitors (PPIs) have demonstrated anticancer and hypoglycemic effects, but their mechanisms of action are not yet fully understood. We used the GeneCards and PharmMapper databases to identify therapeutic targets for diabetes, breast cancer and PPIs. We identified common targets and constructed a regulatory network of diseases and drugs using the STRING database and Cytoscape software. We also explored the binding between small molecule ligands and protein receptors using Discovery Studio software. We identified 33 shared targets for breast cancer, diabetes, and PPIs including lansoprazole, omeprazole, and pantoprazole, which play a critical role in fatty acid transport, insulin resistance, apoptosis, and cancer-related signaling pathways. Our findings demonstrated that PPIs had a strong affinity for AKT1 and MMP9. This study provides insights into the mechanisms of action of PPIs in breast cancer and diabetes and identifies AKT1 and MMP9 as critical targets for future drug development. Our findings highlight the potential of PPIs as a novel therapeutic approach for these challenging diseases.
Topics: Humans; Female; Proton Pump Inhibitors; Matrix Metalloproteinase 9; Anti-Ulcer Agents; Breast Neoplasms; Network Pharmacology; Diabetes Mellitus; 2-Pyridinylmethylsulfinylbenzimidazoles
PubMed: 37165049
DOI: 10.1038/s41598-023-34524-x -
Inflammopharmacology Jun 2016The black cumin or Nigella sativa L. seeds have many acclaimed medicinal properties such as bronchodilatory, hypotensive, antibacterial, antifungal, analgesic,... (Review)
Review
The black cumin or Nigella sativa L. seeds have many acclaimed medicinal properties such as bronchodilatory, hypotensive, antibacterial, antifungal, analgesic, anti-inflammatory and immunopotentiating. This review article is an update on the previous article published on Nigella sativa L. in this journal in 1999. It covers the medicinal properties and chemical syntheses of the alkaloids isolated from the seeds of the herb.
Topics: Analgesics; Animals; Anti-Ulcer Agents; Antineoplastic Agents; Humans; Nigella sativa; Plant Extracts; Seeds
PubMed: 27068721
DOI: 10.1007/s10787-016-0262-7 -
The Korean Journal of Gastroenterology... Nov 2020The Korean guidelines for the Clinical Guidelines for Drug-related Peptic Ulcer were revised under the Korean College of Helicobacter and Upper Gastrointestinal Research... (Review)
Review
The Korean guidelines for the Clinical Guidelines for Drug-related Peptic Ulcer were revised under the Korean College of Helicobacter and Upper Gastrointestinal Research in 2020. In these revised guidelines, treatment for infections is recommended in patients with a history of peptic ulcers and are receiving long-term low-dose aspirin therapy to prevent peptic ulcers and complications. The maintenance of anti-ulcer drugs, such as proton pump inhibitors, is also recommended after eradication if patients require other antiplatelet agents or anticoagulants. Regardless of eradication, when patients with a history of peptic ulcer take long-term low dose aspirin, the concomitant use of a proton pump inhibitor according to the severity of the peptic ulcer is recommended.
Topics: Anti-Inflammatory Agents, Non-Steroidal; Anti-Ulcer Agents; Aspirin; Helicobacter Infections; Helicobacter pylori; Humans; Peptic Ulcer; Platelet Aggregation Inhibitors; Proton Pump Inhibitors
PubMed: 33234770
DOI: 10.4166/kjg.2020.140 -
International Journal of Biological... Dec 2020This study investigated the gastroprotective effect of Lycium barbarum polysaccharides (LBP) and C-phycocyanin (C-PC) in rats with ethanol-induced gastric ulcer. Rats...
This study investigated the gastroprotective effect of Lycium barbarum polysaccharides (LBP) and C-phycocyanin (C-PC) in rats with ethanol-induced gastric ulcer. Rats were divided into 5 groups: normal, ulcer, ulcer treated with 100 mg/kg bw LBP, ulcer treated with 50 mg/kg bw C-PC, and ulcer treated with 50 mg/kg bw LBP and 25 mg/kg bw C-PC. Pretreatment with LBP and/or C-PC was given a week before ulcer induction. Ulcer induction was produced by 50% ethanol administration orally every other day for 4 weeks. After 5-week treatment, the histopathological observation showed that LBP or C-PC attenuated the severity of gastric mucosal damage. LBP decreased serum malondialdehyde (MDA) levels and gastric interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1) levels, and myeloperoxidase (MPO) activity. C-PC decreased serum MDA levels and gastric tumor necrosis factor-α (TNF-α), IL-1β, IL-6, ICAM-1 levels, and MPO activity. Combined LBP and C-PC decreased serum MDA levels and gastric TNF-α, IL-1β, IL-6, and ICAM-1 levels. LBP and/or C-PC increased gastric heat shock protein 70 and non-protein sulfhydryl compounds. Rats with ulcer and treatment had enriched with the family Bacillaceae. Therefore, pretreatment with LBP and/or C-PC attenuated ethanol-induced gastric ulcer in rats via suppressing oxidation and inflammation and increasing gastroprotection.
Topics: Animals; Anti-Ulcer Agents; Ethanol; Gastric Mucosa; Humans; Lycium; Oxidative Stress; Phycocyanin; Plant Extracts; Polysaccharides; Protective Agents; Rats; Stomach; Stomach Ulcer
PubMed: 33058973
DOI: 10.1016/j.ijbiomac.2020.10.037 -
Journal of Ethnopharmacology Mar 2019Virola elongata is a tree species belonging to the Myristicaceae family, distributed in the North and Midwest regions of Brazil, in the phytogeographic domain of the...
ETHNOPHARMACOLOGICAL RELEVANCE
Virola elongata is a tree species belonging to the Myristicaceae family, distributed in the North and Midwest regions of Brazil, in the phytogeographic domain of the Amazon. The aqueous infusion or the hydroethanolic macerate of the stem bark of V. elongata are used in Brazilian and Ecuadorian indigenous folk medicine for several ethnopharmacological purposes, principally, in the treatment of stomach pain, indigestions, and gastric ulcers. This study was aimed to investigate the gastroprotective activity of this plant in order to support its popular use with scientific evidence.
MATERIALS AND METHODS
The stem bark hydroethanolic extract of the plant (HEVe) was prepared by maceration. Its qualitative and quantitative phytochemical constituents were investigated by classical colorimetric techniques, HPLC, and electrospray ionization-multiple stage fragmentation (ESI-MS). The gastroprotective and antiulcer activity of HEVe at doses of 100, 300 and 900 mg/kg p.o. were tested using three acute (acidified ethanol, piroxicam, and in-water-restrain stress), and one chronic (acetic acid) animal ulcer models. The probable mode of action of the HEVe was evaluated by analyzing gastric acid secretion, mucus content, nitric oxide effect, and its antioxidant properties (on catalase, myeloperoxidase, and GSH content) in experimental rodents. The direct extract's activity on the growth of Helicobacter pylori was also investigated.
RESULTS
Total phenolic content in the HEVe was of 146.20 ± 1.07 mg, being flavonoids about 50% (71.79 ± 0.70 mg) of it. Comparative HPLC fingerprint analysis revealed the presence of known phenolic antiulcer compounds, such as gallic acid, catechin, and rutin. Also, methanol/water fractionation and ESI-MS analysis of the HEVe reveals the presence of quinic acid, 3,3',4-trihydroxystilbene, juruenolid D, one catechin dimer, one C-glycosyl flavonoid, one polyketide and two neolignans as the major components of the extract. The HEVe attenuated gastric ulceration in all the different models of acute gastric ulcer, by enhancing gastroprotection through its antioxidant properties in vivo, and reducing also considerably the gastric secretion and total acidity. The HEVe also presented healing properties against the induced chronic ulceration process. On the other hand, the HEVe did not exhibit direct activity against H. pylori.
CONCLUSION
The HEVe exhibited significant gastroprotective/antiulcer effects and contain a relative high proportion of phenolic compounds, especially flavonoids, that could likely account, at least in part, for its pharmacological properties. The results justify its traditional usage and provided scientific evidence for its potential as a new herbal medicine to treat gastric ulcers.
Topics: Acetic Acid; Animals; Anti-Ulcer Agents; Ethanol; Female; Mice; Myristicaceae; Phytochemicals; Phytotherapy; Plant Bark; Plant Extracts; Rats, Wistar; Solvents; Stomach Ulcer
PubMed: 30415060
DOI: 10.1016/j.jep.2018.11.011 -
Pharmaceutical Biology Dec 2017The resin from the trunk wood of Virola oleifera (Schott) A. C. Smith (Myristicaceae) is used in folk medicine to hasten wound repair and to treat pain and inflammatory...
CONTEXT
The resin from the trunk wood of Virola oleifera (Schott) A. C. Smith (Myristicaceae) is used in folk medicine to hasten wound repair and to treat pain and inflammatory conditions, and our previous report indicated the anti-oxidative properties in other oxidative stress model.
OBJECTIVE
To investigate the protective effects of resin from V. oleifera in two experimental models of gastric ulcer oxidative-stress dependent.
MATERIALS AND METHODS
Plant material was collected and the resin was subjected to partitioning with organic solvents. The buthanol fraction was subjected to chromatographic and spectrometric methods for isolation and structural elucidation. The resin was quantified for polyphenols and flavonoids by colorimetric methods. Furthermore, the antioxidant activity of resin was determined by three different methods. The ulcers were induced acutely in Swiss male mice with ethanol/HCl and indomethacin using single-doses of 10 and 100 mg/kg. The gastroprotection of the experimental groups was comparable to reference control lansoprazole (3 mg/kg).
RESULTS
The high content of polyphenols (∼82%) and the presence of epicatechin and eriodictyol were determined. The LD was estimated at 2500 mg/kg. At minimum (10 mg/kg) and maximum (100 mg/kg) dosage of resin, both in ethanol/HCl as indomethacin ulcer induction models demonstrate reduction of lesions (minimum: ∼97% and ∼66%; maximum: ∼95% and ∼59%).
DISCUSSION
The gastroprotection might be related to tannins, phenolic acids and flavonoids present in the resin by antioxidant properties.
CONCLUSIONS
The results indicate that this resin has gastroprotective activity probably associated with the presence of phenolic antioxidant substances.
Topics: Animals; Anti-Ulcer Agents; Antioxidants; Benzothiazoles; Chromatography, Thin Layer; Disease Models, Animal; Ethanol; Flavonoids; Gastric Mucosa; Hydrochloric Acid; Indomethacin; Lethal Dose 50; Male; Mice; Myristicaceae; Phytotherapy; Plant Bark; Plant Extracts; Plants, Medicinal; Polyphenols; Resins, Plant; Solvents; Spectrometry, Mass, Electrospray Ionization; Stomach Ulcer; Sulfonic Acids; Tandem Mass Spectrometry
PubMed: 27937036
DOI: 10.1080/13880209.2016.1251467