-
Microbiology Spectrum Mar 2024This study aims to summarize the clinical characteristics and prognosis of (LM) meningitis in children in Chongqing, China. A retrospective analysis of the clinical...
This study aims to summarize the clinical characteristics and prognosis of (LM) meningitis in children in Chongqing, China. A retrospective analysis of the clinical data and follow-up results of 20 LM meningitis patients admitted to the Children's Hospital of Chongqing Medical University (CHCMU) from January 2012 to December 2022 was performed. The LM meningitis incidence rate was between 0 and 14.3 per 100,000 persons. The median age at onset was 8.98 months. There were five neonate cases, which all had perinatal abnormalities. Seven non-neonatal cases (7/15, 46.7%) had a documented history of contaminated food intake. One case had primary immunodeficiency. The most common symptoms were fever (20/20), altered consciousness (19/20), and vomiting (15/20). Seven cases had seizures, eight cases had cranial nerve involvement, eight cases had positive Babinski sign, and 10 cases had positive meningeal signs. The most common complications were hyponatremia (6/20), hypokalemia (6/20), respiratory failure (5/20), subdural effusion (3/20), and hydrocephalus (2/20). Treatment primarily involved monotherapy or combination therapy with meropenem (15/20) and ampicillin (10/20). Fifteen cases were treated with monotherapy or combination therapy using vancomycin. Twelve cases were successfully followed up from 10 months to 9 years and 6 months, and all had favorable long-term outcomes. LM meningitis incidence in children is low and with nonspecific clinical manifestations. Strengthening food hygiene and safety education, and avoiding infections during pregnancy are important to prevent LM infection in neonates and high-risk individuals. Meropenem and ampicillin are the preferred treatments. Early diagnosis and treatment can improve prognosis.IMPORTANCEThe incidence of LM meningitis is extremely low, and there is currently no standardized treatment. We conducted a retrospective analysis of ten years of data from CHCMU regarding diagnosed LM meningitis cases, aiming to provide clinical evidence for the diagnosis and treatment.
Topics: Infant, Newborn; Pregnancy; Female; Humans; Child; Infant; Meningitis, Listeria; Meropenem; Anti-Bacterial Agents; Retrospective Studies; Ampicillin; Prognosis; China; Hospitals
PubMed: 38275295
DOI: 10.1128/spectrum.03244-23 -
Revista Argentina de Microbiologia Jun 2024Human listeriosis is an infectious disease caused by Listeria monocytogenes. The invasive form of this disease leads to a high rate of hospitalizations and fatality. The...
Human listeriosis is an infectious disease caused by Listeria monocytogenes. The invasive form of this disease leads to a high rate of hospitalizations and fatality. The main mode of transmission is through contaminated ready-to-eat foods such as dairy, vegetables and meat products. The knowledge of the diversity and population dynamics of isolates collected from human and food sources is essential for the detection of clusters and the identification of common sites of infection. The aim of this study was the molecular characterization of L. monocytogenes isolates in Argentina. We sequenced a total of 63 isolates, 35 from human and 28 from food sources, collected between 2018 and 2023. Our genomic study divided the isolates into two lineages, four serogroups, 17 sequence types and 15 clonal complexes (CCs). The hypervirulent clone CC1 (lineage I; serogroup IVb) predominated in human and food samples. The phylogenomic analysis showed a high and possible epidemiological relationship between isolates from human and/or food sources, suggesting the presence of transmission chains in our country. These findings highlight the need to strengthen genomic surveillance of L. monocytogenes in Argentina. The identification of geographic distribution and characteristics of predominant and emerging clones from human and food sources might help to focus action plans and public health policies better directed at the control and prevention of listeriosis.
PubMed: 38834434
DOI: 10.1016/j.ram.2024.03.002 -
Heliyon Apr 2024Listeriosis is a disease caused by , a relevant microorganism as a causative agent of foodborne diseases - FBD. This study aimed to evaluate the distribution of spp.,...
Listeriosis is a disease caused by , a relevant microorganism as a causative agent of foodborne diseases - FBD. This study aimed to evaluate the distribution of spp., and in different production areas in two small plants (A and B) and two micro-food processing plants (C and D) producing meat derivatives, located in different cities of Colombia. The methodology implemented was . The analysis of sampling points is based on a harmonised tool. . Four samplings in each production plant between 2019 and 2020. . Isolation and identification of microorganisms through conventional microbiology, a semi-automated system, molecular serotyping and clonal characterisation by ERIC-PCR. frequency in the production plants belonging to the study ranged between 5.9 and 28.6 %; for spp., plants A and D had isolated, plant A had the highest proportion, while for geno-serotypes found were: 1/2a, 1/2c, 4a-4c, 4b, 4d - 4e, with geno-serotype 4b as the most frequent. Furthermore, possible persistent isolates were detected in plant C as the feasible sources of contamination, based on failures in flow management, raw material contaminated with lack of standardised cooking processes and transfer of the microorganism through equipment and surfaces. Finally, in three of the four production plants assayed, or spp. were present in the packaging area in some of the samples taken during the study, which calls for increased and frequent monitoring, as well as constant technical support for the control of in micro and small-scale production plants.
PubMed: 38596116
DOI: 10.1016/j.heliyon.2024.e28662 -
International Journal of Microbiology 2024South Africa recently (2017-18) experienced the largest outbreak of human listeriosis in the world caused by following the consumption of "polony," a ready-to-eat meat...
Detection of Pathogenic Serogroups and Virulence Genes in Strains Isolated from Beef and Beef Products Retailed in Gauteng Province, South Africa, Using Phenotypic and Polymerase Chain Reaction (PCR)-Based Methods.
South Africa recently (2017-18) experienced the largest outbreak of human listeriosis in the world caused by following the consumption of "polony," a ready-to-eat meat product. Most (59%) cases originated from Gauteng province, South Africa. As a follow-up study to the outbreak, we used standard bacteriological and molecular methods to determine the prevalence of pathogenic and virulent serogroups of in various beef and beef products retailed in Gauteng province, South Africa. The overall prevalence of spp. was 28% (112/400), comprising (9.3%), (16.3%), and (2.5%) ( < 0.001). It is crucial to have detected that the region (=0.036), type of product (=0.032), and temperature at storage (=0.011) significantly affected the occurrence of in beef products. It is alarming that pathogenic serogroups 4b-4d-4e (51.4%) and 1/2a-3a (43.2%) were detected among the isolates of . Importantly, they were all carriers of seven virulence-associated genes (). Our study also demonstrated that 16.7% of "polony" samples investigated were contaminated with Considering that pathogenic and virulent contaminated beef and beef products retailed in South Africa, the food safety risk posed to consumers remains and cannot be ignored. Therefore, it is imperative to reduce the contamination of these products with during beef production, processing, and retailing to avoid future outbreaks of human listeriosis in the country.
PubMed: 38510936
DOI: 10.1155/2024/8891963 -
Swiss Medical Weekly May 2024Listeriosis is a notifiable disease in Switzerland. In summer 2022, the Swiss Federal Office of Public Health noticed an increase in reports of listeriosis cases,...
AIMS OF THE STUDY
Listeriosis is a notifiable disease in Switzerland. In summer 2022, the Swiss Federal Office of Public Health noticed an increase in reports of listeriosis cases, indicating a possible ongoing outbreak. Here we present the approaches applied for rapidly confirming the outbreak, detecting the underlying source of infection and the measures put in place to eliminate it and contain the outbreak.
METHODS
For close surveillance and early detection of outbreak situations with their possible sources, listeriosis patients in Switzerland are systematically interviewed about risk behaviours and foods consumed prior to the infection. Listeria monocytogenes isolates derived from patients in medical laboratories are sent to the National Reference Laboratory for Enteropathogenic Bacteria and Listeria, where they routinely undergo whole-genome sequencing. Interview and whole-genome sequencing data are continuously linked for comparison and analysis.
RESULTS
In summer 2022, 20 patient-derived L. monocytogenes serotype 4b sequence type 388 strains were found to belong to an outbreak cluster (≤10 different alleles between neighbouring isolates) based on core genome multilocus sequence typing analysis. Geographically, 18 of 20 outbreak cases occurred in northeastern Switzerland. The median age of patients was 77.4 years (range: 58.1-89.7), with both sexes equally affected. Rolling analysis of the interview data revealed smoked trout from a local producer as a suspected infection source, triggering an on-site investigation of the production facility and sampling of the suspected products by the responsible cantonal food inspection team on 15 July 2022. Seven of ten samples tested positive for L. monocytogenes and the respective cantonal authority ordered a ban on production and distribution as well as a product recall. The Federal Food Safety and Veterinary Office released a nationwide public alert covering the smoked fish products concerned. Whole-genome sequencing analysis confirmed the interrelatedness of the L. monocytogenes smoked trout product isolates and the patient-derived isolates. Following the ban on production and distribution and the product recall, reporting of new outbreak-related cases rapidly dropped to zero.
CONCLUSIONS
This listeriosis outbreak could be contained within a relatively short time thanks to identification of the source of contamination through the established combined approach of timely interviewing of every listeriosis patient or a representative and continuous molecular analysis of the patient- and food-derived L. monocytogenes isolates. These findings highlight the effectiveness of this well-established, joint approach involving the federal and cantonal authorities and the research institutions mandated to contain listeriosis outbreaks in Switzerland.
Topics: Humans; Switzerland; Disease Outbreaks; Listeria monocytogenes; Listeriosis; Whole Genome Sequencing; Male; Aged; Female; Aged, 80 and over; Multilocus Sequence Typing; Middle Aged; Food Microbiology; Foodborne Diseases; Interviews as Topic
PubMed: 38701492
DOI: 10.57187/s.3745 -
Foods (Basel, Switzerland) May 2024() is an opportunistic foodborne pathogen that causes listeriosis in humans and animals, reaching up to 30% case mortality. There are only a few reports in Mexico...
() is an opportunistic foodborne pathogen that causes listeriosis in humans and animals, reaching up to 30% case mortality. There are only a few reports in Mexico about the strains found in various foods. The aim of this study was to determine the prevalence of , serogroups, virulence genes, and antimicrobial resistance in different foods from Reynosa, Tamaulipas, Mexico. strains were characterized by microbiological and molecular methods. Susceptibility to 12 antibiotics was determined according to CLSI and EUCAST. A total of 300 samples of seafood, pasteurized and raw milk, cheese, beef, and chicken were collected from supermarkets and retail markets. The presence of was detected in 5.6% of the samples. Most strains belonged to serogroups 4b, 4d, and 4e (68.4%). All strains presented a minimum of four virulence genes; the most common were , , and (92.1%). A high percentage of antimicrobial susceptibility was observed, with resistance only to STX-TMP (78.9%), STR (26.3%), MEM (21.0%), and E (2.6%). These results show that the foods in Reynosa, Tamaulipas, are a reservoir of and represent a potential health risk.
PubMed: 38890883
DOI: 10.3390/foods13111656 -
International Journal of Infectious... Aug 2024Listeria monocytogenes (LM) is a health threat worldwide given its high mortality and the growing of high-risk susceptible populations.
OBJECTIVES
Listeria monocytogenes (LM) is a health threat worldwide given its high mortality and the growing of high-risk susceptible populations.
METHODS
All hospitalizations with a diagnosis of LM in the National Registry of Hospital Discharges were examined in Spain from 2000 to 2021.
RESULTS
A total of 8152 hospital admissions with LM were identified. The mean age was 59.5 years and 48% were immunosuppressed (IS). The rate of LM hospitalizations increased from 5 per 1 million population in 2000 to 8.9 in 2021 (p < 0.001). A foodborne outbreak in Andalusia determined a sharp increase in admissions with LM during 2019. The COVID-19 pandemic and lockdowns were associated with a decrease in LM admissions. The overall in-hospital mortality was 16.7%. The number of deaths in patients hospitalized with LM rose from 7.8 per 100,000 deceased in 2000 to 18 in 2021 (p < 0.001). After adjustment, age >65 years (odds ratio [OR] = 2.16), sepsis (OR = 2.60), meningoencephalitis (OR = 1.72), endocarditis (OR = 2.0), neonatal listeriosis (OR = 2.10) and IS (OR = 2.09) were associated with mortality.
CONCLUSIONS
The number of patients hospitalized with LM in Spain has increased significantly from 2000 to 2021. The increase in the rate of admissions and deaths was largely driven by the growing proportion of elderly and IS patients.
Topics: Humans; Listeriosis; Spain; Middle Aged; Male; Aged; Female; Listeria monocytogenes; Incidence; Hospital Mortality; Hospitalization; Adult; COVID-19; Aged, 80 and over; Young Adult; Adolescent; Disease Outbreaks; Child; Child, Preschool; Infant; Risk Factors
PubMed: 38734058
DOI: 10.1016/j.ijid.2024.107089 -
Heliyon Jan 2024To report and analyze the clinical characteristics of 15 patients with Listeria meningitis in adult.
OBJECTIVE
To report and analyze the clinical characteristics of 15 patients with Listeria meningitis in adult.
METHODS
We reviewed the medical records of 15 patients with Listeria meningitis who were admitted to Shanxi Bethune Hospital between January 2017 and January 2023.
RESULTS
The clinical manifestations was primarily characterized by fever, altered mental status, headache, neck stiffness, and vomiting. Blood or cerebrospinal fluid (CSF) cultures were performed in 15 cases, and pathogens were detected in 11 of them. Metagenomic next-generation sequencing (mNGS) detected pathogens in 10 cases, with four being negative by conventional methods and six being positive through traditional tests. The laboratory blood results presented leukocytosis. The CSF analysis upon admission showed elevated levels of white blood cells and proteins, as well as decreased chloride and glucose concentration. The brain computed tomography (CT) revealed ventricular enlargement in 3 patients. The brain magnetic resonance imaging (MRI) showed abnormalities in multiple areas of the brain. Despite 3 patients with decompensated hydrocephalus underwent lateral ventricle puncture and drainage,their neurological deterioration were increasingly deteriorating.7 patients were treated by mechanical ventilation due to respiratory insufficiency. After 3 months, there were 9 cases with excellent outcomes(modified Rankin Scale score of 0-2),2 cases with favorable outcomes(score of 3-5), and 4 deaths(score of 6).
CONCLUSIONS
This thesis found that the detection rate of has been on a rise over the past six years in our department, ranking second only to . Additionally, the detection rate achieved by mNGS surpasses that of other conventional methods. Among the patient cohort, 11 had underlying diseases such as systemic lupus erythematosus, tuberculosis, diabetes mellitus, pituitary neoplasms, leukemia and other related illnesses. Once listeriosis is early identified, the adequate antibiotic therapy should be promptly introduced in the course of empirical treatment.
PubMed: 38223708
DOI: 10.1016/j.heliyon.2023.e23755 -
Journal of Veterinary Research Sep 2023is an important foodborne pathogen responsible for human listeriosis, which is a disease with high hospitalisation and mortality rates. The bacteria are usually...
INTRODUCTION
is an important foodborne pathogen responsible for human listeriosis, which is a disease with high hospitalisation and mortality rates. The bacteria are usually susceptible to most antibacterial substances, but resistance to some of them has been recently observed. The present study introduces the evidence on the emergence of antibiotic resistance among strains isolated from food and food-production environments in Poland.
MATERIAL AND METHODS
A total of 283 isolates classified into serogroups IIa and IVb which had been recovered from food and food production environments were tested with 17 antimicrobials. These included those that are recommended for treatment of severe listeriosis cases in humans. A multiplex PCR was used to identify serogroups, and a microbroth dilution method was applied for the determination of antibiotic resistance among the isolates tested.
RESULTS
Only 34 (12.0%) strains were susceptible to all the antimicrobials used in the study. The remaining 249 (88.0%) strains displayed different instances of resistance to the antimicrobials tested, from insusceptibility to one (112 strains; 39.6%) to resistance to four antibacterial substances (6 strains; 2.1%). Among them, there were 38 strains (13.4%) with multiresistance patterns.
CONCLUSION
Polish food and its processing environments may be a potential source of antimicrobial-resistant , which may pose a potential health risk to consumers in the country.
PubMed: 37786847
DOI: 10.2478/jvetres-2023-0050 -
Microbial Genomics Jan 2024is a food-borne pathogen, typically affecting the elderly, immunocompromised patients and pregnant women. The aim of this study was to determine the population...
is a food-borne pathogen, typically affecting the elderly, immunocompromised patients and pregnant women. The aim of this study was to determine the population structure of clonal complex 1 (CC1) in the UK and describe the genomic epidemiology of this clinically significant CC. We interrogated a working dataset of 4073 sequences of isolated between January 2015 and December 2020 from human clinical specimens, food and/or food-production environments. A minimum spanning tree was reconstructed to determine the population structure of in the UK. Subsequent analysis focused on CC1, as the cause of the highest proportion of invasive listeriosis in humans. Sequencing data was integrated with metadata on food and environmental isolates, and information from patient questionnaires, including age, sex and clinical outcomes. All isolates either belonged to lineage I (=1299/4073, 32%) or lineage II (=2774/4073, 68%), with clinical isolates from human cases more likely to belong to lineage I (=546/928, 59%) and food isolates more likely to belong to lineage II (=2352/3067, 77%). Of the four largest CCs, CC1 (=237) had the highest proportion of isolates from human cases of disease (CC1 =160/237, 67.5 %; CC121 =13/843, 2 %; CC9 =53/360, 15 %; CC2 =69/339, 20%). Within CC1, most cases were female (=95/160, 59%, =0.01771) and the highest proportion of cases were in people >60 years old (39/95, 41%, =1.314×10) with a high number of them aged 20-39 years old (=35/95, 37%) most linked to pregnancy-related listeriosis (=29/35, 83%). Most of the male cases were in men aged over 60 years old (40/65, 62%), and most of the fatal cases in both males and females were identified in this age group (42/55, 76%). Phylogenetic analysis revealed 23 5 SNP single linkage clusters comprising 80/237 (34 %) isolates with cluster sizes ranging from 2 to 19. Five 5 SNP clusters comprised isolates from human cases and an implicated food item. Expanding the analysis to 25 SNP single linkage clusters resolved an additional two clusters linking human cases to a potential food vehicle. Analysis of demographic and clinical outcome data identified CC1 as a clinically significant cause of invasive listeriosis in the elderly population and in women of child-bearing age. Phylogenetic analysis revealed the population structure of CC1 in the UK comprised small, sparsely populated genomic clusters. Only clusters containing isolates from an implicated food vehicle, or food processing or farming environments, were resolved, emphasizing the need for clinical, food and animal-health agencies to share sequencing data in real time, and the importance of a One Health approach to public-health surveillance of listeriosis.
Topics: Pregnancy; Animals; Male; Humans; Female; Aged; Middle Aged; Young Adult; Adult; Listeria monocytogenes; Phylogeny; Genomics; Listeriosis; United Kingdom
PubMed: 38165396
DOI: 10.1099/mgen.0.001155