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Ear, Nose, & Throat Journal Dec 2023We describe a case of nasal non-Hodgkin's lymphoma in a 79-year-old Chinese patient accompany with nasal myiasis. The first 2 biopsies in this case were false negative....
We describe a case of nasal non-Hodgkin's lymphoma in a 79-year-old Chinese patient accompany with nasal myiasis. The first 2 biopsies in this case were false negative. Subsequently, nasal maggots developed in this patient. After removing all maggots under nasal endoscopy, the patient continued to have recurrent fever and was transferred to a higher hospital for further treatment, in which he received a third biopsy. Unfortunately, several hours after the biopsy, the patient died for severe nasal bleeding. The final biopsy result indicated the neoplasm of the left nasal cavity was non-Hodgkin's lymphoma. This case illustrates the importance of repeated biopsies for nasal non-Hodgkin's lymphoma if necessary. Nasal myiasis is a secondary disease of nasal non-Hodgkin's lymphoma in this case.
Topics: Male; Humans; Aged; Hodgkin Disease; Lymphoma, Non-Hodgkin; Myiasis
PubMed: 34233486
DOI: 10.1177/01455613211031024 -
Journal of Insect Science (Online) Sep 2023Ham products play a fundamental role in the Italian economy, and attention to the problems of this sector is essential. The products of this sector can be attacked by...
Evaluation of Pachycrepoideus vindemiae and Muscidifurax raptor (Hymenoptera: Pteromalidae) as biological control agents of Piophila casei (Diptera: Piophilidae) in ham production facilities.
Ham products play a fundamental role in the Italian economy, and attention to the problems of this sector is essential. The products of this sector can be attacked by parasites, which can cause direct and indirect damage. Piophila casei (L.) (Diptera: Piophilidae) a cheese and meat parasite, is currently responsible for hygiene problems in ham factories. The trophic activity of this pest on the products causes serious direct damage and it is a vector of various bacteria, including Clostridium botulinum. Another risk is human ingestion of the larvae, which are resistant to gastric juices action, potentially causing intestinal myiasis. Insecticide use of any type is not allowed in aging rooms, so biological control can represent a potential alternative. In this study, we investigate quality parameters such as successful rate of parasitism (SP), degree of parasitism (DP), sex-ratio (SR), life-span (LS), and emergence rates (ER) of 2 pupal parasitoids of Diptera: Pachycrepoideus vindemiae (Rondani) (Hymenoptera: Pteromalidae), currently the only known pupal parasitoid of P. casei, and Muscidifurax raptor (Girault and Sanders) (Hymenoptera: Pteromalidae). Our research confirmed P. vindemiae efficacy to parasitize P. casei and reported, for the first time, M. raptor as a pupal parasitoid of this Piophilidae. ER for both parasitoids were low, thus affecting the DP and SP estimations. This could be explained by the feeding behavior of the parasitoid host. The strongly female-biased SR for P. vindemiae supported previous studies. LS results in our experiment are crucial for determining the timing of release.
Topics: Animals; Humans; Female; Diptera; Hymenoptera; Biological Control Agents; Larva; Feeding Behavior
PubMed: 37721495
DOI: 10.1093/jisesa/iead067 -
Oxford Medical Case Reports Nov 2023Myiasis is defined as the infestation of any part of the body by fly larvae. It is particularly common in tropical and subtropical regions. Cutaneous myiasis is the most...
Myiasis is defined as the infestation of any part of the body by fly larvae. It is particularly common in tropical and subtropical regions. Cutaneous myiasis is the most common manifestation of this infestation. Here, we report a 21-year-old Syrian female who presented with a 10-day history of painful 2 ulcer-like lesions on her scalp and was diagnosed with furuncular myiasis, which included more than 20 larvae. The patient had no history of international travel to myiasis-endemic areas before the onset of the lesions. She probably acquired the infestation while visiting a cattle farm located in a rural region east of Hama governorate. Seborrheic dermatitis developed on her scalp after the myiasis treatment was performed.
PubMed: 38033409
DOI: 10.1093/omcr/omad126 -
BMC Genomics Jan 2024Wohlfahrtia magnifica is an obligatory parasite that causes myiasis in several warm-blooded vertebrates. Adult females deposit the first-stage larvae directly onto...
BACKGROUND
Wohlfahrtia magnifica is an obligatory parasite that causes myiasis in several warm-blooded vertebrates. Adult females deposit the first-stage larvae directly onto wounds or natural body orifices (e.g., genitalia) of the host, from where they quickly colonize the host tissue and feed on it for development. The infestation of W. magnifica can lead to health issues, welfare concerns, and substantial economic losses. To date, little is known about the molecular mechanisms of the W. magnifica-causing myiasis.
RESULTS
In this study, we collected parasitic-stage larvae of W. magnifica from wounds of naturally infested Bactrian camels, as well as pupae and adult flies reared in vitro from the wound-collected larvae, for investigating the gene expression profiles of the different developmental stages of W. magnifica, with a particular focus on examining gene families closely related to the parasitism of the wound-collected larvae. As key proteins related to the parasite-host interaction, 2049 excretory/secretory (ES) proteins were identified in W. magnifica through the integration of multiple bioinformatics approaches. Functional analysis indicates that these ES proteins are primarily involved in cuticle development, peptidase activity, immune response, and metabolic processes. The global investigation of gene expression at different developmental stages using pairwise comparisons and weighted correlation network analysis (WGCNA) showed that the upregulated genes during second-stage larvae were related to cuticle development, peptidase activity, and RNA transcription and translation; during third-stage larvae to peptidase inhibitor activity and nutrient reservoir activity; during pupae to cell and tissue morphogenesis and cell and tissue development; and during adult flies to signal perception, many of them involved in light perception, and adult behavior, e.g., feeding, mating, and locomotion. Specifically, the expression level analysis of the likely parasitism-related genes in parasitic wound-collected larvae revealed a significant upregulation of 88 peptidase genes (including 47 serine peptidase genes), 110 cuticle protein genes, and 21 heat shock protein (hsp) genes. Interestingly, the expression of 2 antimicrobial peptide (AMP) genes, including 1 defensin and 1 diptericin, was also upregulated in the parasitic larvae.
CONCLUSIONS
We identified ES proteins in W. magnifica and investigated their functional distribution. In addition, gene expression profiles at different developmental stages of W. magnifica were examined. Specifically, we focused on gene families closely related to parasitism of wound-collected larvae. These findings shed light on the molecular mechanisms underlying the life cycle of the myiasis-causing fly, especially during the parasitic larval stages, and provide guidance for the development of control measures against W. magnifica.
Topics: Animals; Female; Sarcophagidae; Parasites; Myiasis; Diptera; Larva; Pupa; Gene Expression Profiling; Peptide Hydrolases
PubMed: 38297211
DOI: 10.1186/s12864-023-09949-3 -
Revista Brasileira de Parasitologia... 2024Oestrus ovis larvae are obligate parasites of vertebrates and cause cavitary myiasis (oestrosis) in sheep and goats. It is also reported as a zoonosis causing...
Oestrus ovis larvae are obligate parasites of vertebrates and cause cavitary myiasis (oestrosis) in sheep and goats. It is also reported as a zoonosis causing ophthalmomyiasis and nasopharyngeal myiasis. Despite being relatively common in Brazil, epidemiological studies on O. ovis are scarce. Considering that the infestation is influenced by the climate and biomes of the studied region, we aimed to investigate the seasonal prevalence of O. ovis among slaughtered sheep in the northern region of the state of Mato Grosso, Brazil. The heads of sheep (n=697) slaughtered at a slaughterhouse in the municipality of Terra Nova do Norte (November 2011 to November 2013) were collected to count, catalog, and identify the larvae found in the upper respiratory tract. Overall, 45.77% (319/697) of the animals were infested with 2,412 recovered larvae, 96.89% (2,337/2,412) of which were identified at the species level as O. ovis. Seasonal variations in prevalence ranged from 41% (spring) to 56% (summer); however, no correlation was observed between prevalence and season, mean humidity, or temperature. In conclusion, parasitism by O. ovis in sheep in the studied area, occurs year-round, considering the occurrence of larvae (L1, L2, and L3) throughout the year, probably because of the area's environmental conditions.
Topics: Animals; Brazil; Sheep; Seasons; Prevalence; Myiasis; Sheep Diseases; Diptera; Larva
PubMed: 38656051
DOI: 10.1590/S1984-29612024020 -
Nihon Ronen Igakkai Zasshi. Japanese... 2024A 75-year-old man with type 2 diabetes and a history of previous empyema surgery was admitted to our hospital due to difficulty moving caused by chronic obstructive...
A 75-year-old man with type 2 diabetes and a history of previous empyema surgery was admitted to our hospital due to difficulty moving caused by chronic obstructive pulmonary disease and dehydration. During the first two days of hospitalization, intestinal myiasis was diagnosed after maggots were found in his diapers. After the maggots disappeared, he developed a fever, prompting antibiotic therapy for a suspected secondary infection, resulting in clinical improvement. Despite thorough home cleaning, no flies or maggots were found, and the source of infection and the fly species remained unknown. Recent reports suggest a higher prevalence of myiasis among the elderly, even with overall improvement in hygiene. While myiasis is typically mild, it is a condition that requires consideration in an aging society. Myiasis is a disease that should be considered in the differential diagnosis of the elderly, especially in people who are bedridden or frail.
Topics: Male; Animals; Humans; Aged; Diabetes Mellitus, Type 2; Anti-Bacterial Agents; Myiasis; Diagnosis, Differential; Larva
PubMed: 38583974
DOI: 10.3143/geriatrics.61.80 -
Tropical Medicine and Infectious Disease Nov 2023Myiasis is one of the most common skin diseases found in travelers returning from tropical and subtropical regions, where humans living in or visiting the African...
Myiasis is one of the most common skin diseases found in travelers returning from tropical and subtropical regions, where humans living in or visiting the African continent are most commonly infested by during the rainy season in regions with a warm climate. Here, we present a case of furuncular myiasis caused by in a Serbian patient returning from temporary work in Kenya, where the initial histology of skin lesion mimicked hyperproliferative skin disorder.
PubMed: 37999624
DOI: 10.3390/tropicalmed8110505 -
Parasites & Vectors Mar 2024In Rio Grande do Sul, Brazil, a region with a subtropical climate, Rhipicephalus microplus is present in taurine cattle raised for beef and milk. In addition, ticks...
BACKGROUND
In Rio Grande do Sul, Brazil, a region with a subtropical climate, Rhipicephalus microplus is present in taurine cattle raised for beef and milk. In addition, ticks resistant to multiple acaricides are present in this region. Recently, fluralaner (isoxazoline) was launched on the market. Thus, there is a need to evaluate the effects of fluralaner for the control of R. microplus on taurine cattle. In addition, occurrence of myiasis by Cochliomyia hominivorax larvae after tick parasitism and weight gain of cattle during the experimental period were evaluated.
METHODS
Thirty naturally infested cattle were divided into two experimental groups: T01, treated with fluralaner (2.5 mg/kg) pour-on; T02, control. T01 received fluralaner on Days 0 (early summer in January), 42 and 84 (early autumn), whereas T02, a control group, received palliative treatment with a spray formulation when the group mean was ≥ 30 ticks. Counts of R. microplus females and calculation of the efficacy of fluralaner were performed on Days 3, 7, 14, 28, 35, 42, 56, 70, 84, 98, 112 and 126. The occurrence of myiasis was assessed throughout the study period. In addition, the weight, weight gain and daily weight gain of the animals were evaluated.
RESULTS
In the 12 evaluations performed, the parasitic load of T01 was near zero. Fluralaner showed 99.5% efficacy on the 3rd day after the first treatment and 100% efficacy from Day 7 to Day 126. Cochliomyia hominivorax larvae (n = 6; p = 0.0251) were found only in the control group (T02). At the end of the study, the animals subjected to treatments with fluralaner gained 32.8 kg more than the animals in the control group.
CONCLUSIONS
Application of fluralaner in summer and autumn, with 42-day intervals between treatments, was effective to control R. microplus on taurine cattle, which also gained more weight than control cattle. Additionally, no cases of myasis were documented in animals treated with fluralaner.
Topics: Female; Cattle; Animals; Rhipicephalus; Tick Infestations; Myiasis; Larva; Calliphoridae; Weight Gain; Cattle Diseases; Isoxazoles
PubMed: 38429835
DOI: 10.1186/s13071-024-06200-0 -
Genes Apr 2024The Australian sheep blowfly, , is a major sheep ectoparasite causing subcutaneous myiasis (flystrike), which can lead to reduced livestock productivity and, in severe...
The Australian sheep blowfly, , is a major sheep ectoparasite causing subcutaneous myiasis (flystrike), which can lead to reduced livestock productivity and, in severe instances, death of the affected animals. It is also a primary colonizer of carrion, an efficient pollinator, and used in maggot debridement therapy and forensic investigations. In this study, we report the complete mitochondrial (mt) genome of from the Northern Territory (NT), Australia, where sheep are prohibited animals, unlike the rest of Australia. The mt genome is 15,943 bp in length, comprising 13 protein-coding genes (PCGs), two ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs), and a non-coding control region. The gene order of the current mt genome is consistent with the previously published mt genomes. Nucleotide composition revealed an AT bias, accounting for 77.5% of total mt genome nucleotides. Phylogenetic analyses of 56 species/taxa of dipterans indicated that and are the closest among all sibling species of the genus , which helps to explain species evolution within the family Luciliinae. This study provides the first complete mt genome sequence for derived from the NT, Australia to facilitate species identification and the examination of the evolutionary history of these blowflies.
Topics: Animals; Genome, Mitochondrial; Calliphoridae; Phylogeny; Northern Territory; Myiasis; RNA, Transfer; RNA, Ribosomal; Diptera; Sheep
PubMed: 38674440
DOI: 10.3390/genes15040506 -
Parasites & Vectors Sep 2023This study describes the effectiveness of a novel active pharmaceutical ingredient, fluralaner (isoxazoline class), against important ectoparasites infesting cattle in...
BACKGROUND
This study describes the effectiveness of a novel active pharmaceutical ingredient, fluralaner (isoxazoline class), against important ectoparasites infesting cattle in Brazil.
METHODS
A total of 13 studies involving a 5% fluralaner-based pour-on formulation (Exzolt 5%; further referred to as Exzolt) were conducted. Specifically, the effectiveness of this formulation was studied against Rhipicephalus microplus (6 studies), Cochliomyia hominivorax larvae (4 studies), Dermatobia hominis larvae (1 study) and Haematobia irritans flies (2 studies).
RESULTS
The therapeutic efficacy of Exzolt was found to exceed 98% at 4 days post treatment (DPT), while persistent efficacy (> 90% efficacy) against repeated infestations of R. microplus was observed for up to 79 DPT. In field studies, ≥ 98% therapeutic efficacy was demonstrated at all study sites by 7 DPT, and a persistent efficacy (> 90% efficacy) was observed for 42, 49 or 56 DPT. Exzolt prevented C. hominivorax eggs from developing to the larval stage, thus mitigating the development of myiasis in cattle naturally and artificially infested with this screworm. The efficacy of Exzolt against D. hominis larvae was 98% at 3 DPT, while persistent efficacy (> 90% effectiveness) was found to last for up to 70 DPT. Against H. irritans, Exzolt showed therapeutic efficacy (≥ 90%) within the first day of treatment at both study sites, while persistent efficacy (≥ 90%) was observed for 7 DPT at one site and for 21 DPT at the other site.
CONCLUSIONS
Overall, the results from these studies confirm that Exzolt is therapeutically efficacious against the most important ectoparasites infesting cattle in Brazil. The novel active pharmaceutical ingredient, fluralaner, provides a new treatment option for farmers to control cattle ectoparasites, especially where there is resistance to other chemical classes. In addition, an effective control of ectoparasites will improve overall cattle health and well-being as well as production.
Topics: Animals; Cattle; Brazil; Ovum; Muscidae; Larva; Cattle Diseases; Pharmaceutical Preparations; Tick Infestations
PubMed: 37752574
DOI: 10.1186/s13071-023-05934-7