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Acta Veterinaria Scandinavica Dec 2022Although left and right displacement of the abomasum and abomasal volvulus are well-known disorders of cattle, a comparative evaluation of the clinical, laboratory and...
BACKGROUND
Although left and right displacement of the abomasum and abomasal volvulus are well-known disorders of cattle, a comparative evaluation of the clinical, laboratory and ultrasonographic findings of these types of abomasal displacements has not been undertaken. Therefore, the objective of this study was to compare these conditions in a large population of cows. The medical records of 1982 dairy cows with left displaced abomasum (LDA, n = 1341), right displaced absomasum (RDA, n = 338) and abomasal volvulus (AV, n = 303) were searched for the results of clinical, laboratory and ultrasonographic evaluations.
RESULTS
The main clinical findings were an abnormal demeanour in 48.2% of the cows, reduced rumen motility in 89.7% and decreased intestinal motility in 61.1%. Ballottement and simultaneous auscultation and percussion and simultaneous auscultation were positive on the left side in 96.9% of the cows with LDA and on the right in 98.5% of the cows with RDA and in 99.3% of the cows with AV. Ultrasonography was useful for diagnosing LDA in 97.9% of the cows and RDA/AV in 90.2% of the cows. The laboratory findings characteristic of abomasal reflux syndrome varied in severity; 83% of the cows had hypokalaemia, 67% had increased rumen chloride concentration, 67% had an increased base excess and 50% had haemoconcentration. Based on the clinical signs, a definitive diagnosis was made in 75.0% of the cows with LDA and in 22.5% of the cows with RDA/AV. Ultrasonography was required for a definitive diagnosis in another 22.0% of the cows with LDA and in 53.0% of the cows with RDA/AV. Laparotomy or postmortem examination was required for reliable differentiation of RDA and AV.
CONCLUSIONS
LDA, RDA and AV differ with respect to the severity and the frequency of several abnormal clinical, laboratory and ultrasonographic findings as well as the methods required for a diagnosis.
Topics: Female; Cattle; Animals; Pregnancy; Intestinal Volvulus; Abomasum; Stomach Diseases; Rumen; Cattle Diseases
PubMed: 36539887
DOI: 10.1186/s13028-022-00656-9 -
The Veterinary Clinics of North... Mar 2018Clostridial abomasitis and enteritis are important alimentary diseases observed in all domestic ruminant species. These diseases most commonly result from overgrowth of... (Review)
Review
Clostridial abomasitis and enteritis are important alimentary diseases observed in all domestic ruminant species. These diseases most commonly result from overgrowth of Clostridium perfringens types A, B, C, D, and E with the associated release of bacterial exotoxins that result in necrosis of the abomasal or intestinal mucosa. Clostridium difficile may also be associated with enteritis in calves but is much less common than disease caused by C perfringens. This article reviews the causes, pathophysiology, clinical signs, diagnosis, treatment, and prevention of clostridial gastrointestinal diseases in ruminants. Particular emphasis is given to describing the various forms of disease and treatment of individual cases.
Topics: Abomasum; Animals; Cattle; Cattle Diseases; Clostridium Infections; Clostridium perfringens; Enteritis; Gastrointestinal Diseases; Goat Diseases; Goats; Sheep; Sheep Diseases
PubMed: 29421028
DOI: 10.1016/j.cvfa.2017.10.010 -
Cell Transplantation Feb 2018The interstitial cells of Cajal (ICCs) are regarded as pacemakers and are involved in neurotransmission in the gastrointestinal tract (GIT) of animals. However, limited...
The interstitial cells of Cajal (ICCs) are regarded as pacemakers and are involved in neurotransmission in the gastrointestinal tract (GIT) of animals. However, limited information is available about the existence of ICCs within the GIT of ruminants. In this study, we investigated the ultrastructural characteristics and distribution of ICCs in goat abomasum using transmission electron microscopy and c-kit immunohistochemistry. Two different kinds of c-kit immunoreactive cells were observed in the abomasum. The first was identified as ICCs, which appeared to be multipolar or bipolar in shape, with some processes. These c-kit immunoreactive cells were deposited in the submucosal layer, myenteric plexus between the circular and longitudinal muscle layers, and within the longitudinal and circular muscle layers of the abomasum. The second type of cell was round in shape and was identified as mast cells, which were located in the submucosal layer as well as in the lamina propria. Ultrastructurally, ICCs were also observed as stellate or spindle-shaped cells, which were consistent in shape with our c-kit immunoreactive cells. In the cytoplasm of ICCs, numerous mitochondria, rough endoplasmic reticulum, and caveolae were detected. ICCs were located in the myenteric plexus between the longitudinal and circular muscle layers (ICC-MY), with the longitudinal and circular muscle layer was replaced as "intramuscular layers" (ICC-IM), and in the submucosal layer (ICC-SM). In addition, we found ICCs surrounding nerve fibers and smooth muscle cells, where they formed heterocellular junctions in the form of close membrane associations or gap junctions and homocellular junctions among the processes of the ICCs. In the current study, we provide the first complete characterization of ICCs within the goat abomasum and propose that ICCs might have a key role in producing contractions in the ruminant stomach for proper absorption of nutrients.
Topics: Abomasum; Animals; Female; Goats; Immunohistochemistry; Interstitial Cells of Cajal; Male; Microscopy, Electron, Transmission; Myenteric Plexus
PubMed: 28933185
DOI: 10.1177/0963689717722561 -
The Veterinary Clinics of North... Nov 2009Ultrasonography is an ideal diagnostic tool for investigating gastrointestinal disorders in cattle. In animals with traumatic reticuloperitonitis, inflammatory fibrinous... (Review)
Review
Ultrasonography is an ideal diagnostic tool for investigating gastrointestinal disorders in cattle. In animals with traumatic reticuloperitonitis, inflammatory fibrinous changes and abscesses can be imaged. Ultrasonography can be used to assess the size, position, and contents of the abomasum. This article describes the ultrasonographic techniques used for examination of the reticulum, rumen, omasum, abomasum, small intestine, and large intestine. The normal findings are presented followed by a description of the most important diseases of these organs.
Topics: Abomasum; Animals; Cattle; Cattle Diseases; Diagnosis, Differential; Gastrointestinal Diseases; Gastrointestinal Tract; Rumen; Ultrasonography
PubMed: 19825434
DOI: 10.1016/j.cvfa.2009.07.004 -
Schweizer Archiv Fur Tierheilkunde Mar 2022An oesophageal tube feeder was used to administer milk to six clinically healthy calves every 12 hours for a total of three feedings. The calves were seven to nine days...
An oesophageal tube feeder was used to administer milk to six clinically healthy calves every 12 hours for a total of three feedings. The calves were seven to nine days of age, and each feeding consisted of a volume of milk that was 6 % of the body weight of the calves. The reticulum and abomasum were scanned ultrasonographically before, during and after drenching. Blood samples for D-lactate determination and venous blood gas analysis were collected at -12, 0 (first drenching), 6, 12 (second drenching), 18, 24 (third drenching), 30 and 36 hours. Ruminal fluid was collected for measurement of pH and D-lactate concentration at -12 and 36 hours. Based on simultaneous ultrasonographic examinations of the reticulum and abomasum, some of the force-fed milk flowed into the reticulum and some directly into the abomasum; both organs contained milk immediately after drenching. Drenching led to ruminal acidosis evidenced by a significant decrease in median pH from 7,0 (6,0 - 7,0) to 4,5 (3,0 - 5,0) and a 93-fold increase in median D-lactate concentration from 0,39 (0,14 - 1,33) to 36,3 (17,9 - 53,3) mmol/l but it did not cause metabolic acidosis. Based on these findings, we concluded that milk administered to calves via an oesophageal tube feeder flows into the reticulum and abomasum simultaneously and three force feedings 12 hours apart cause acute ruminal acidosis, which was not accompanied by metabolic acidosis in the short term.
Topics: Abomasum; Animals; Cattle; Diet; Endoplasmic Reticulum Stress; Hydrogen-Ion Concentration; Lactic Acid; Milk; Rumen
PubMed: 35232715
DOI: 10.17236/sat00347 -
BMC Veterinary Research Oct 2022Due to the complexity of ruminant digestion, cannulation of organs of the digestive tract has been carried out in order to advance the understanding of digestive...
BACKGROUND
Due to the complexity of ruminant digestion, cannulation of organs of the digestive tract has been carried out in order to advance the understanding of digestive physiology, nutrient degradability, gastrointestinal diseases and biotechnological research. The abomasal cannulation is interesting for nutritional studies, especially in suckling calves, to obtain fluid and abomasal content, evaluation of abomasal flow and function, and infusion of nutrients and drugs when it is intended to reach high concentrations in the organ. Conventionally, access and cannulation of digestive organs of ruminants has been performed by laparotomy, a method often criticized and classified as cruel by some sectors related to ethics and animal welfare. The aim of this present study is to describe and standardize a minimally invasive by laparoscopy assisted abomasal cannulation in bovine fetuses (cadavers), which had been previously slaughtered by accident and would be discarded in local slaughterhouses.
RESULTS
The abomasal cannulation technique was feasible, simple and did not present major difficulties. The surgical time for cannulation of the abomasum, from the insertion of the trocars to the completion of the technique with fixation of the organ to the abdominal wall, ranged from 9 to 27 min, with an average of 15.5 ± 6.62 min.
CONCLUSIONS
The Laproscopic assisted abomasal cannulation in bovine fetuses was feasible and safe with minimal tissue injury to the abdominal wall and with short surgical time. More studies in the clinical routine related to minimally invasive abomasal content collection, abomasopexy and abomasotomy are required in order to demonstrate its impact and importance in bovine clinic.
Topics: Cattle; Animals; Abomasum; Laparoscopy; Catheterization; Fetus; Cadaver
PubMed: 36284310
DOI: 10.1186/s12917-022-03473-4 -
Invited review: Abomasal emptying in calves and its potential influence on gastrointestinal disease.Journal of Dairy Science Jan 2017Creating the ideal nutrition program for calves is a demanding task that has undergone tremendous change in recent years. Products and technologies including novel milk... (Review)
Review
Creating the ideal nutrition program for calves is a demanding task that has undergone tremendous change in recent years. Products and technologies including novel milk replacers and automated calf feeding systems have been developed to facilitate the ability of dairy producers to feed for higher growth rates before weaning. The creation of new feeding programs and milk replacers has to be looked at carefully, not only from a nutrition point of view but also from the perspective of a potential effect on physiologic digestion and calf health. Abomasal emptying is a critical factor that may link nutrition and disease. The purpose of this article is to review both intrinsic and extrinsic factors that are responsible for abomasal emptying. Predominant extrinsic factors controlling abomasal emptying include meal volume, energy density, and osmolality along with the content and source of protein. This article also reviews experimental methods used to measure abomasal emptying in the calf including those that would be appropriate for use under field conditions. Among these methods, the use of ultrasonography and different absorption tests (d-xylose, acetaminophen) as tools to measure abomasal emptying are discussed. The relationship between abomasal emptying and disease is explored, particularly as it relates to abomasal bloat. Abomasal bloat is a complex syndrome that seems to be increasing in frequency and whose etiology likely at least partially involves slowing of abomasal emptying. Suggestions for minimizing the effect of feeding programs on abomasal emptying are explored as well as needs for future research.
Topics: Abomasum; Absorption, Physiological; Acetaminophen; Animals; Cattle; Cattle Diseases; Female; Gastric Emptying; Gastrointestinal Diseases; Ultrasonography; Xylose
PubMed: 27865497
DOI: 10.3168/jds.2016-10949 -
Microbiology Spectrum Dec 2022Left displaced abomasum (LDA) in postpartum dairy cows contributes to significant economic losses. Dairy cows with LDA undergo excessive lipid mobilization and insulin...
Left displaced abomasum (LDA) in postpartum dairy cows contributes to significant economic losses. Dairy cows with LDA undergo excessive lipid mobilization and insulin resistance. Although gut dysbiosis is implicated, little is known about the role of the gut microbiota in the abnormal metabolic processes of LDA. To investigate the functional links among microbiota, metabolites, and disease phenotypes in LDA, we performed 16S rDNA gene amplicon sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS) of fecal samples from cows with LDA ( = 10) and healthy cows ( = 10). Plasma marker profiling was synchronously analyzed. In the LDA event, gut microbiota composition and fecal metabolome were shifted in circulation with an amino acid pool deficit in dairy cows. Compared with the healthy cows, salicylic acid derived from microbiota catabolism was decreased in the LDA cows, which negatively correlated with , , non-esterified fatty acid (NEFA), and β-hydroxybutyric acid (BHBA) levels. Conversely, fecal taurolithocholic acid levels were increased in cows with LDA. Based on integrated analysis with the plasma metabolome, eight genera and eight metabolites were associated with LDA. Of note, the increases in and abundances were negatively correlated with the decreases in 4-pyridoxic acid and cytidine levels, and positively correlated with the increases in NEFA and BHBA levels in amino acid deficit, indicating pyridoxal metabolism-associated gut dysbiosis and lipolysis. Changes in branched-chain amino acids implicated novel host-microbial metabolic pathways involving lipolysis and insulin resistance in cows with LDA. Overall, these results suggest an interplay between host and gut microbes contributing to LDA pathogenesis. LDA is a major contributor to economic losses in the dairy industry worldwide; however, the mechanisms associated with the metabolic changes in LDA remain unclear. Most previous studies have focused on the rumen microbiota in terms of understanding the contributors to the productivity and health of dairy cows; this study further sheds light on the relevance of the lower gut microbiota and its associated metabolites in mediating the development of LDA. This study is the first to characterize the correlation between gut microbes and metabolic phenotypes in dairy cows with LDA by leveraging multi-omics data, highlighting that the gut microbe may be involved in the regulation of lipolysis and insulin resistance by modulating the amino acid composition. Moreover, this study provides new markers for further research to understand the pathogenesis of the disease as well as to develop effective treatment and prevention strategies.
Topics: Female; Cattle; Animals; Insulin Resistance; Chromatography, Liquid; Abomasum; Fatty Acids, Nonesterified; Dysbiosis; Tandem Mass Spectrometry; Metabolome; Stomach Diseases; Gastrointestinal Microbiome
PubMed: 36222683
DOI: 10.1128/spectrum.01972-22 -
Veterinary Research 2003The research with respect to displacement of the abomasum (DA) in dairy cattle is reviewed. Evaluated articles describe epidemiological and experimental studies. The... (Review)
Review
The research with respect to displacement of the abomasum (DA) in dairy cattle is reviewed. Evaluated articles describe epidemiological and experimental studies. The occurrence is elevated with regard to breed, gender, age, concurrent diseases, environmental aspects and production levels as contributing factors and emphasis is placed on the effects of nutrition and metabolism. Reviewing the experimental work, distinction is made between the research into gas production in the abomasum and hypomotility of the abomasum, since both represent presumed pathways in the development of DA. Although the different fields of research have positive contributions to the understanding of the pathogenesis of DA, contradictions in the different studies are present. This is partly due to extrapolation of results from sheep to cows, or because of a low number of cows in the experiments. Finally, general suggestions are made for further research in the field of the pathogenesis of DA.
Topics: Abomasum; Animal Nutritional Physiological Phenomena; Animals; Calcium; Cattle; Cattle Diseases; Dairying; Risk Factors
PubMed: 12588683
DOI: 10.1051/vetres:2002060 -
Veterinary Parasitology Jul 2022Santa Ines, an indigenous Brazilian hair sheep, in comparison with European sheep breeds (e.g., Ile de France), show greater resistance against gastrointestinal nematode...
Santa Ines, an indigenous Brazilian hair sheep, in comparison with European sheep breeds (e.g., Ile de France), show greater resistance against gastrointestinal nematode (GIN) infections, mainly to Haemonchus contortus. Here we bring novel findings that address some gaps regarding the resistance traits involved in the development of the immunity of young lambs in the first few weeks of life to H. contortus infection. This study aimed to compare parasitological, humoral, and local effector cell-mediated responses, also the histopathological differences in the abomasal mucosa of Santa Ines (SI) and Ile de France (IF) suckling lambs serially infected with H. contortus. Parasitological variables, local and circulating humoral immunity, and local cellular response were evaluated in naïve Santa Ines (n = 14) and Ile de France (n = 12) lambs, randomized into four groups: infected SI (n = 8), non-infected control SI (n = 6), infected IF (n = 8) and non-infected control IF (n = 4). Lambs from infected groups were first infected at 14 days old, and multiple infections were conducted every second day, until the age of 66 days old (52 days post first infection). In comparison with infected Ile de France, infected Santa Ines lambs had lower mean eggs per gram of faeces, lower total H. contortus worm burden, lower females' length, greater abomasal lymph node weight, greater mucosal thickness in the fundus, and also higher counts of eosinophils in the fundus, and mast cells and globule leukocytes in both fundic and pyloric mucosa of the abomasum. Intrabreed differences were observed into the infected Santa Ines group, with three of the eight lambs classified as highly resistant for displaying H. contortus burden ranging only from 1 to 42 worms. Overall, Santa Ines suckling lambs showed great resistance against H. contortus infection in comparison with Ile de France lambs, being able to mount a robust innate immune response at an early age, and before weaning.
Topics: Abomasum; Animals; Disease Susceptibility; Feces; Female; Gastrointestinal Diseases; Haemonchiasis; Haemonchus; Immunity, Humoral; Nematode Infections; Parasite Egg Count; Sheep; Sheep Diseases
PubMed: 35660130
DOI: 10.1016/j.vetpar.2022.109734