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Antimicrobial Resistance and Infection... Apr 2023The use of disinfectants and alcohol-based hand rubs (ABHR) to prevent COVID-19 transmission increased in the first wave of the infection. To meet the increased demand,...
BACKGROUND
The use of disinfectants and alcohol-based hand rubs (ABHR) to prevent COVID-19 transmission increased in the first wave of the infection. To meet the increased demand, the Iranian Ministry of Health issued an emergency use authorization allowing new manufacturers to enter the market, despite the limited capacity for surveillance of these products during COVID-19. Methanol poisoning outbreaks spread rapidly, and more people died from methanol poisoning than COVID-19 in some cities. The aim of this study was to analyze some ABHRs in the Iranian market to see if (a) ABHRs are standard and suitable for hand antisepsis and (b) contained potentially dangerous toxic alcohols.
METHOD
Between February and March 2020, 64 brands of ABHR were conveniently collected from pharmacies, supermarkets, and shops selling hygienic products and analyzed using Gas Chromatography. World Health Organization and Food and Drug Administration guidelines were used to define minimum requirements for ABHR. For estimating the risk for acute methanol poisoning, we assumed a serum methanol concentration of 200 mg/L following ABHR ingestion was sufficient to cause intoxication. This threshold concentration would be achieved in an average 75-kg adult after consuming 8000 mg (or eight grams) methanol in 1-2 h.
RESULTS
The median [IQR] (range) concentration of ethanol, isopropanol, and methanol were 59% v/v [32.2, 68] (0, 99), 0 mg/L [0, 0] (0, 197,961), and 0 mg/L [0, 0] (0, 680,100), respectively. There was a strong negative correlation between methanol and ethanol contents of hand rubbers (r= -0.617, p < 0.001). Almost 47% of ABHRs complied with minimum standards. In 12.5% of ABHRs, high concentrations of methanol were observed, which have no antiseptic properties but could cause acute methanol poisoning if ingested.
CONCLUSION
COVID-19 initiated a policy for distribution and use of ABHR with little control. As ABHR and masks are still accepted preventive measures of the disease, non-standard ABHR compositions may increase the population's risk to both COVID-19 infection and methanol poisoning.
Topics: United States; Adult; Humans; Iran; 2-Propanol; Cross-Sectional Studies; Methanol; Hand Disinfection; COVID-19; Ethanol
PubMed: 37098641
DOI: 10.1186/s13756-023-01244-w -
Journal of Hepatology Aug 2013
Topics: Alcohol Drinking; Ethanol; Humans; Liver Diseases, Alcoholic; Models, Biological; Risk Factors
PubMed: 23391479
DOI: 10.1016/j.jhep.2013.01.035 -
Pharmacology & Therapeutics Nov 2006GABA(A) receptors (GABA(A)Rs) are the main inhibitory neurotransmitter receptors and have long been implicated in mediating at least part of the acute actions of... (Review)
Review
GABA(A) receptors (GABA(A)Rs) are the main inhibitory neurotransmitter receptors and have long been implicated in mediating at least part of the acute actions of ethanol. For example, ethanol and GABAergic drugs including barbiturates and benzodiazepines share many pharmacological properties. Besides the prototypical synaptic GABA(A)R subtypes, nonsynaptic GABA(A)Rs have recently emerged as important regulators of neuronal excitability. While high doses (> or =100 mM) of ethanol have been reported to enhance activity of most GABA(A)R subtypes, most abundant synaptic GABA(A)Rs are essentially insensitive to ethanol concentrations that occur during social ethanol consumption (< 30 mM). However, extrasynaptic delta and beta3 subunit-containing GABA(A)Rs, associated in the brain with alpha4 or alpha6 subunits, are sensitive to low millimolar ethanol concentrations, as produced by drinking half a glass of wine. Additionally, we found that a mutation in the cerebellar alpha6 subunit (alpha6R100Q), initially reported in rats selectively bred for increased alcohol sensitivity, is sufficient to produce increased alcohol-induced motor impairment and further increases of alcohol sensitivity in recombinant alpha6beta3delta receptors. Furthermore, the behavioral alcohol antagonist Ro15-4513 blocks the low dose alcohol enhancement on alpha4/6/beta3delta receptors, without reducing GABA-induced currents. In binding assays alpha4beta3delta GABA(A)Rs bind [(3)H]Ro15-4513 with high affinity, and this binding is inhibited, in an apparently competitive fashion, by low ethanol concentrations, as well as analogs of Ro15-4513 that are active to antagonize ethanol or Ro15-4513's block of ethanol. We conclude that most low to moderate dose alcohol effects are mediated by alcohol actions on alcohol/Ro15-4513 binding sites on GABA(A)R subtypes.
Topics: Acute Disease; Benzodiazepines; Electrophysiology; Ethanol; Humans; Ion Channels; Mutation; Neurons; Polymorphism, Genetic; Receptors, GABA
PubMed: 16814864
DOI: 10.1016/j.pharmthera.2006.05.004 -
International Journal of Molecular... Dec 2023A previously unknown reduction of carbonyl compounds with dicyclopentylzinc is reported. Aldehydes react in mild conditions yielding corresponding primary alcohols and...
A previously unknown reduction of carbonyl compounds with dicyclopentylzinc is reported. Aldehydes react in mild conditions yielding corresponding primary alcohols and cyclopentene. Although cyclohexanone and acetophenone are inert to dicyclopentylzinc, a variety of heterocyclic ketones reacted readily, yielding reasonable to high yields of corresponding secondary alcohols. When the reaction was catalyzed with (-)-(1,2)-ephedrine, 3-acetylpyridine () resulted in a high yield of ()-1-(pyridin-3-yl)ethanol () with >99% ee. 5-Acetyl-2-bromopyridine () also provided the corresponding optically active alcohol , albeit with a much lower optical yield. When 10% of with 92% ee was used as an autocatalyst, 55% yield of the same compound was obtained, with 95% ee and 96% ee in two independent experiments. A three-stage reaction sequence starting from "no chirality" reaction yielded with 6% ee. Thus, amplifying autocatalysis was detected in the reaction of ketone with dicylopentylzinc.
Topics: Catalysis; Stereoisomerism; Aldehydes; Ketones; Ethanol
PubMed: 38069371
DOI: 10.3390/ijms242317048 -
Current Drug Abuse Reviews 2015There is a wealth of research about the links between executive functioning (EF) and alcohol use. However, difficulty may arise in interpreting findings because of the... (Review)
Review
There is a wealth of research about the links between executive functioning (EF) and alcohol use. However, difficulty may arise in interpreting findings because of the variability between studies regarding the specific components of EF measured, as well as the variability of tasks used to examine each EF construct. The current article considers each of these problems within the context of a literature review that focuses on two topics: (1) the efficacy of EF in predicting alcohol use and alcohol-related consequences, and (2) the effect of acute alcohol intoxication on EF task performance. An additional goal was to identify and describe commonly used EF measures with the intention of providing alcohol researchers information on the assessment of different EF domains. Our findings indicate that there is strong evidence supporting a relation between EF difficulties (particularly response inhibition and information updating) and alcohol use, with additional evidence of a significant interaction between EF and implicit associations on alcohol use. In contrast, research supporting a link between set shifting abilities and later alcohol use is scarce. Additionally, this review found evidence of alcohol acutely affecting many EF processes (particularly response inhibition). Overall, there is a need to replicate these findings with commonly used EF tasks (versus developing numerous tasks within individual laboratories) to better advance our understanding of the relation between EF and alcohol use.
Topics: Alcohol Drinking; Alcohol-Related Disorders; Ethanol; Executive Function; Humans; Inhibition, Psychological
PubMed: 25877524
DOI: 10.2174/1874473708666150416110515 -
Addiction Biology Sep 2022Binge alcohol consumption is common among adolescents and may impair normal brain development. Emerging, longitudinal studies in adolescents suggest that the effects of...
Binge alcohol consumption is common among adolescents and may impair normal brain development. Emerging, longitudinal studies in adolescents suggest that the effects of binge alcohol exposure on brain structure differ between sexes. To test the hypothesis that the effects of binge alcohol exposure on developmental brain growth trajectories are influenced by age of exposure and sex, adolescent and adult, male and female C57Bl/6 mice (n = 32), were exposed to a binge-like ethanol (EtOH) exposure paradigm (i.e., 5 cycles of 2 on/2 off days of 5 g/kg EtOH intraperitoneal) or served as saline controls. Longitudinal structural magnetic resonance imaging was acquired at baseline, following binge EtOH exposure, and after 2 weeks of recovery. Alcohol treatment showed interactions with age and sex in altering whole brain volume: adolescents of both sexes demonstrated inhibited whole brain growth relative to their control counterparts, although significance was only attained in female mice which showed a larger magnitude response to EtOH compared to male mice. In region of interest analyses, the somatosensory cortex and cerebellum showed inhibited growth in male and female adolescent mice exposed to EtOH, but the difference relative to controls did not reach multiple comparison-corrected statistical significance. These data suggest that in mice exposed to binge EtOH treatment, adolescent age of exposure and female sex may confer a higher risk to the detrimental effects of EtOH on brain structure and reinforce the need for direct testing of both sexes.
Topics: Animals; Binge Drinking; Brain; Ethanol; Female; Magnetic Resonance Imaging; Male; Mice; Mice, Inbred C57BL
PubMed: 36001428
DOI: 10.1111/adb.13209 -
Neuropsychopharmacology : Official... Oct 2022Models of addiction are based on neurobiological, behavioral, and pharmacological studies in animals, but translational support from human studies is limited. Studies...
Models of addiction are based on neurobiological, behavioral, and pharmacological studies in animals, but translational support from human studies is limited. Studies are lacking in examining acute responses to alcohol in drinkers with alcohol use disorder (AUD), particularly in terms of relevant intoxicating doses and measurement of stimulating and rewarding effects throughout the breath alcohol concentration (BrAC) time curve. Participants were N = 60 AUD drinkers enrolled in the Chicago Social Drinking Project and examined in three random-order and blinded sessions for subjective and physiological responses to a beverage containing 0.0 g/kg, 0.8 g/kg, and 1.2 g/kg alcohol. BrAC in the alcohol sessions at 60 min was 0.09 g/dL and 0.13 g/dL, respectively. Both doses of alcohol produced significant biphasic effects on subjective measures of stimulation, euphoria, reward (liking and wanting), sedation, and neuroendocrine and cardiovascular factors. Increased pleasurable effects of alcohol were pronounced during the rising limb-to-peak BrAC and sedating effects emerged during the declining limb. Alcohol dose-dependently increased feel drug ratings and rewarding effects at peak BrAC or early declining limb, and physiological responses at the rising limb. Thus, rather than the notion of an overall tolerance, results show an alcohol response phenotype characterized by sensitivity to alcohol's stimulating, rewarding and physiological effects. The results of this study may aid in the conceptualization of alcohol addiction as a disorder characterized by the persistence of enhanced hedonic alcohol responses rather than chronic tolerance and reward deficiency.
Topics: Alcohol Drinking; Alcoholism; Breath Tests; Ethanol; Humans; Reward
PubMed: 35701549
DOI: 10.1038/s41386-022-01340-2 -
Molecules (Basel, Switzerland) Jul 2022Ethanol is the most commonly used recreational drug worldwide. This study describes the development and validation of a headspace gas chromatography flame ionisation...
Development of a Gas-Tight Syringe Headspace GC-FID Method for the Detection of Ethanol, and a Description of the Legal and Practical Framework for Its Analysis, in Samples of English and Welsh Motorists' Blood and Urine.
Ethanol is the most commonly used recreational drug worldwide. This study describes the development and validation of a headspace gas chromatography flame ionisation detection (HS-GC-FID) method using dual columns and detectors for simultaneous separation and quantitation. The use of a dual-column, dual-detector HS-GC-FID to quantitate ethanol is a common analytical technique in forensic toxicology; however, most analytical systems utilise pressure-balance injection rather than a simplified gas-tight syringe, as per this technique. This study is the first to develop and validate a technique that meets the specifications of the United Kingdom’s requirements for road traffic toxicology testing using a Shimadzu GC-2014 gas-tight syringe. The calibration ranged from 10 to 400 mg/100 mL, with a target minimum linearity of r2 > 0.999, using tertiary butanol as the internal standard marker. The method has an expanded uncertainty at 99.73% confidence of 3.64% at 80 mg/100 mL, which is the blood alcohol limit for drink driving in England and Wales. In addition, at 200 mg%—the limit at which a custodial sentence may be imposed on the defendant—the expanded uncertainty was 1.95%. For both the 80 mg% and 200 mg% concentrations, no bias was present in the analytical method. This method displays sufficient separation for other alcohols, such as methanol, isopropanol, acetaldehyde, and acetone. The validation of this technique complies with the recommended laboratory guidelines set out by United Kingdom and Ireland Association of Forensic Toxicologists (UKIAFT), the recently issued Laboratory 51 guidelines by the United Kingdom Accreditation Service (UKAS), and the criteria set out by the California Code of Regulations (CCR), 17 CCR § 1220.1.
Topics: Acetaldehyde; Chromatography, Gas; Ethanol; Flame Ionization; Syringes
PubMed: 35897946
DOI: 10.3390/molecules27154771 -
Nature Communications 2013Ethanol alters nerve signalling by interacting with proteins in the central nervous system, particularly pentameric ligand-gated ion channels. A recent series of...
Ethanol alters nerve signalling by interacting with proteins in the central nervous system, particularly pentameric ligand-gated ion channels. A recent series of mutagenesis experiments on Gloeobacter violaceus ligand-gated ion channel, a prokaryotic member of this family, identified a single-site variant that is potentiated by pharmacologically relevant concentrations of ethanol. Here we determine crystal structures of the ethanol-sensitized variant in the absence and presence of ethanol and related modulators, which bind in a transmembrane cavity between channel subunits and may stabilize the open form of the channel. Structural and mutagenesis studies defined overlapping mechanisms of potentiation by alcohols and anaesthetics via the inter-subunit cavity. Furthermore, homology modelling show this cavity to be conserved in human ethanol-sensitive glycine and GABA(A) receptors, and to involve residues previously shown to influence alcohol and anaesthetic action on these proteins. These results suggest a common structural basis for ethanol potentiation of an important class of targets for neurological actions of ethanol.
Topics: Amino Acid Sequence; Anesthetics; Crystallography, X-Ray; Drug Synergism; Ethanol; Humans; Ion Channel Gating; Ion Channels; Ligands; Models, Molecular; Molecular Sequence Data; Molecular Structure; Sequence Homology, Amino Acid
PubMed: 23591864
DOI: 10.1038/ncomms2682 -
Journal of Dairy Science Mar 2007Dairy cows fed silage are subjected to various alcohols and low molecular weight esters. Four lactating Holstein cows fitted with ruminal cannulas and permanent...
Dairy cows fed silage are subjected to various alcohols and low molecular weight esters. Four lactating Holstein cows fitted with ruminal cannulas and permanent indwelling catheters in the hepatic portal vein, hepatic vein, mesenteric vein, and mesenteric artery were used to study the absorption of alcohols into portal blood and the metabolism of feed alcohols in the rumen and splanchnic tissues. The cows were allocated to 4 experimental treatments in a Latin square design. All treatments were formulated as total mixed rations with the same overall nutrient composition, differing by the source of corn silage. Treatments were a control silage and 3 qualities of problematic corn silage (silage with Fusarium toxin, Penicillium-infected silage, and silage with a high propanol content). Feeding was followed by a decreasing ruminal pH, as well as decreasing molar proportions of ruminal acetate and isobutyrate. The ruminal concentrations of total VFA, ethanol, propanol, 2-butanol, ethyl acetate, propyl acetate, glucose, and L-lactate, and molar proportions of propionate, butyrate, isovalerate, valerate, and caproate increased after feeding. Treatments affected ruminal concentrations of propanol, propyl acetate, and butyrate and a strong correlation was observed between ruminal propyl acetate and the molar proportion of butyrate (r = -0.79). Arterial concentrations of ethanol, propanol, propanal, acetone (sum of acetone and acetoacetate), 3-hydroxybutyrate, L-lactate, glutamate, and glutamine increased, and the arterial concentration of glucose decreased after feeding, but no effects of treatment were observed for arterial variables. The postprandial increase in arterial ethanol was maintained for 5 h. The net portal release of ethanol tended to decrease with the treatment with the lowest ethanol content, and the net splanchnic release of ethanol increased after feeding, but overall, the net splanchnic flux of ethanol was not different from zero, in agreement with the liver being the major organ for alcohol metabolism. The net portal flux and net hepatic flux of propanol were affected by treatment. All dietary ethanol and propanol were accounted for by absorption of the respective alcohol into the portal blood. The hepatic extraction ratios of ethanol and propanol were, on average, 63 to 66%, and no indications of saturation of hepatic alcohol metabolism were observed at any time. We concluded that typical amounts of alcohols in corn silage do not interfere with splanchnic metabolism of any of the measured variables and do not saturate hepatic pathways for alcohol metabolism. However, even low concentrations of alcohols in feed might affect ruminal metabolism and are followed by hours of elevated peripheral blood concentrations of alcohols.
Topics: 1-Propanol; Acetates; Animal Feed; Animals; Blood Vessels; Cattle; Dairying; Eating; Ethanol; Female; Fermentation; Glucose; Ketones; Lactation; Lactic Acid; Milk; Rumen; Silage; Time Factors
PubMed: 17297111
DOI: 10.3168/jds.S0022-0302(07)71623-5