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Frontiers in Cellular Neuroscience 2020Bilateral interconnections through the hippocampal commissure play important roles in synchronizing or spreading hippocampal seizure activities. Intact hippocampi or...
Bilateral interconnections through the hippocampal commissure play important roles in synchronizing or spreading hippocampal seizure activities. Intact hippocampi or bilateral hippocampal slices have been isolated from neonatal or immature rats (6-7 or 12-21 days old, respectively) and the mechanisms underlying the bilateral synchrony of hippocampal epileptiform activities have been investigated. However, the feasibility of examining bilateral epileptiform activities of more developed hippocampal circuitry remains to be explored. For this, we prepared bilateral hippocampal slices from C57 black mice, a strain commonly used in neuroscience and for genetic/molecular modifications. Young mice (21-24-day-old) were used in most experiments. A 600-μm-thick slice was obtained from each mouse by horizontal vibratome sectioning. Bilateral dorsal hippocampal and connecting dorsal hippocampal commissure (DHC) tissues were preserved in the slice and extrahippocampal tissues were removed. Slices were recorded in a submerged chamber mainly at a room temperature (21-22°C). Bilateral CA3 areas were monitored by extracellular recordings, and unilateral electrical stimulation was used to elicit CA3 synaptic field potentials. The unilateral stimulation could elicit population spikes in the contralateral CA3 area. These contralateral spikes were attenuated by inhibiting synaptic transmission with cobalt-containing medium and were abolished when a cut was made at the DHC. Self-sustained and bilaterally correlated epileptiform potentials were observed following application of 4-aminopyradine and became independent after the DHC cut. Bilateral hippocampal activities were detectable in some slices of adult mice and/or at 35-36°C, but with smaller amplitudes and variable waveforms compared to those observed from slices of young mice and at the room temperature. Together, these observations suggested that examining bilateral epileptiform activities in hippocampal slices of young mice is feasible. The weaknesses and limitations of this preparation and our experimentation are discussed.
PubMed: 33551747
DOI: 10.3389/fncel.2020.593840 -
American Journal of Ophthalmology Case... Jun 2022We present the case of a 71-year-old male who developed decreased visual acuity eleven years after uncomplicated bilateral cataract extraction and in-the-bag IOL...
PURPOSE
We present the case of a 71-year-old male who developed decreased visual acuity eleven years after uncomplicated bilateral cataract extraction and in-the-bag IOL implantation following recent bouts of vigorous eye rubbing.
OBSERVATIONS
On examination, the posterior capsules were ruptured centrally in both eyes, and there was anterior vitreous prolapse bilaterally. While both IOLs remained in the visual axis, there was mild bilateral decentration and resultant decreased vision.
CONCLUSION AND IMPORTANCE
To the authors' knowledge, this is the first reported case of bilateral simultaneous posterior capsule rupture with anterior vitreous prolapse due to eye rubbing. Contrasting to previous case reports, this scenario demonstrates that severe eye rubbing can have variable severity and complications. Frequent and vigorous eye rubbing can be a precipitating cause of late postoperative posterior capsule rupture and should be avoided in pseudophakic patients, especially those with older IOL models.
PubMed: 35243163
DOI: 10.1016/j.ajoc.2022.101426 -
Journal of Applied Physiology... Mar 2021Phrenic nerve damage may occur as a complication of specific surgical procedures, prolonged mechanical ventilation, or physical trauma. The consequent diaphragmatic...
Phrenic nerve damage may occur as a complication of specific surgical procedures, prolonged mechanical ventilation, or physical trauma. The consequent diaphragmatic paralysis or dysfunction can lead to major complications. The purpose of this study was to elucidate the role of the nondiaphragmatic respiratory muscles during partial or complete diaphragm paralysis induced by unilateral and bilateral phrenic nerve damage at different levels of ventilatory pressure support in an animal model. Ten pigs were instrumented, the phrenic nerve was exposed from the neck, and spontaneous respiration was preserved at three levels of pressure support, namely, high, low, and null, at baseline condition, after left phrenic nerve damage, and after bilateral phrenic nerve damage. Breathing pattern, thoracoabdominal volumes and asynchrony, and pressures were measured at each condition. Physiological breathing was predominantly diaphragmatic and homogeneously distributed between right and left sides. After unilateral damage, the paralyzed hemidiaphragm was passively dragged by the ipsilateral rib cage muscles and the contralateral hemidiaphragm. After bilateral damage, the drive to and the work of breathing of rib cage and abdominal muscles increased, to compensate for diaphragmatic paralysis, ensuing paradoxical thoracoabdominal breathing. Increasing level of pressure support ventilation replaces this muscle group compensation. When the diaphragm is paralyzed (unilaterally and/or bilaterally), there is a coordinated reorganization of nondiaphragmatic respiratory muscles as compensation that might be obscured by high level of pressure support ventilation. Noninvasive thoracoabdominal volume and asynchrony assessment could be useful in phrenic nerve-injured patients to estimate the extent and type of inspiratory muscle dysfunction. This was the first (to our knowledge) implanted porcine model of phrenic nerve injury with a detailed multidimensional analysis of different degrees of diaphragmatic paralysis (unilateral and bilateral). Noninvasive thoracoabdominal volume and asynchrony assessment was shown to be useful in estimating the extent of diaphragmatic dysfunction and the consequent coordinated reorganization of nondiaphragmatic respiratory muscles. High level of pressure support ventilation was proved to obscure the interaction and compensation of respiratory muscles to deal with phrenic nerve injury.
Topics: Animals; Diaphragm; Humans; Intercostal Muscles; Phrenic Nerve; Respiratory Muscles; Respiratory Paralysis; Swine
PubMed: 33444121
DOI: 10.1152/japplphysiol.00781.2020 -
Cureus Apr 2022A 92-year-old female with poorly controlled systemic hypertension presented with bilateral eye redness, lid fullness, conjunctival chemosis, ophthalmoplegia, and ptosis...
A 92-year-old female with poorly controlled systemic hypertension presented with bilateral eye redness, lid fullness, conjunctival chemosis, ophthalmoplegia, and ptosis for two days. A neuro-ophthalmic evaluation revealed bilateral proptosis, severe conjunctival chemosis and congestion, and an almost complete bilateral ophthalmoplegia with a complete right superior eyelid ptosis. Computed tomography (CT) scans demonstrated bilateral dilation of the superior ophthalmic veins, and a CT angiography (CTA) showed a direct high-flow carotid-cavernous fistula (CCF) with secondary extraocular muscle enlargement. Clinicians should be aware that a typical direct high-flow CCF, although usually occurs after trauma and unilaterally, can present spontaneously without trauma and bilaterally.
PubMed: 35664394
DOI: 10.7759/cureus.24634 -
Annals of the Royal College of Surgeons... Feb 2022Symptomatic bilateral juxtafacet ganglion cysts are relatively uncommon in the degenerated spine. The literature describes 16 cases of bilateral ganglion or synovial...
Symptomatic bilateral juxtafacet ganglion cysts are relatively uncommon in the degenerated spine. The literature describes 16 cases of bilateral ganglion or synovial cysts, none reported sciatica and neurogenic claudication simultaneously. We present a case of a 60-year-old woman who presented with symptoms of bilateral sciatica and neurogenic claudication. Magnetic resonance imaging of the lumbar spine revealed bilateral lesions related to the facet joints at the L4/5 level, causing bilateral lateral recess stenosis and narrowing of the central canal due to encroachment of these bilateral lesions at the same level. She underwent an elective central canal decompression of the L4/5 level and excision of the facet cysts bilaterally with lateral recess decompression, which resulted in good relief of both the radicular and claudication symptoms.
Topics: Constriction, Pathologic; Female; Ganglion Cysts; Humans; Lumbar Vertebrae; Magnetic Resonance Imaging; Middle Aged; Sciatica; Synovial Cyst
PubMed: 34414791
DOI: 10.1308/rcsann.2021.0064 -
Journal of Clinical and Experimental... Feb 2023To evaluate bilateral symmetry and anatomical variations of root morphology of permanent mandibular second molar using systematic evaluation of CBCT scans.
BACKGROUND
To evaluate bilateral symmetry and anatomical variations of root morphology of permanent mandibular second molar using systematic evaluation of CBCT scans.
MATERIAL AND METHODS
This cross-sectional study involved the mandible which were imaged using serial axial cone-beam computed tomography (CBCT) from 680 North Indian patients who visited dental hospital for various reasons unrelated to the study. CBCT Records with presence of bilateral fully erupted permanent mandibular second molars with fully formed apexes were selected.
RESULTS
Bilaterally present two root and three canal configurations were most consistently seen (75.88 % and 59.11 %, respectively). The occurrence of two roots with two canals and four canals was 15.14% and 1.61% respectively. Mandibular second molar present with one extra root, radix entomolaris with three canals and four canals was 0.44% and 3.53% while radix paramolaris present with three canals and four canals 1.32% and 1.03% respectively. The occurrence of C-shaped root bilaterally with C shaped canal was 15.88% whereas the presence of one fused root bilaterally was 0.44%. The presence of four roots bilaterally with four canals was identified in only one CBCT scanned image (0.14%). The frequency distribution of root morphology on bilateral symmetrical analysis revealed 98.58 % bilateral symmetry.
CONCLUSIONS
In 402 CBCT scans, bilateral presence of two roots with three canals was the most typical root structure seen in mandibular second molars (59.11%). A rare variation found was the presence of four roots occurring bilaterally, seen in only 1 CBCT scan. Bilateral symmetrical analysis of root morphology revealed 98.58 % bilateral symmetry. Anatomic root variations, mandibular second molar, Cone Beam Computed Tomography scans, bilateral symmetry.
PubMed: 36911156
DOI: 10.4317/jced.60063 -
Frontiers in Endocrinology 2018The functional capacity of the testes in prepubertal boys with cryptorchidism before treatment has received very little attention. The assessment of testicular function...
INTRODUCTION
The functional capacity of the testes in prepubertal boys with cryptorchidism before treatment has received very little attention. The assessment of testicular function at diagnosis could be helpful in the understanding of the pathophysiology of cryptorchidism and in the evaluation of the effect of treatment. Anti-Müllerian hormone is a well-accepted Sertoli cell biomarker to evaluate testicular function during childhood without the need for stimulation tests.
OBJECTIVE
The aim of the study was to assess testicular function in prepubertal children with cryptorchidism before orchiopexy, by determining serum anti-Müllerian hormone (AMH). We also evaluated serum gonadotropins and testosterone and looked for associations between testicular function and the clinical characteristics of cryptorchidism.
MATERIALS AND METHODS
We performed a retrospective, cross-sectional, analytical study at a tertiary pediatric public hospital. All clinical charts of patients admitted at the outpatient clinic, and recorded in our database with the diagnosis of cryptorchidism, were eligible. The main outcome measure of the study was the serum concentration of AMH. Secondary outcome measures were serum LH, FSH, and testosterone. For comparison, serum hormone levels from a normal population of 179 apparently normal prepubertal boys were used.
RESULTS
Out of 1,557 patients eligible in our database, 186 with bilateral and 124 with unilateral cryptorchidism were selected using a randomization software. Median AMH standard deviation score was below 0 in both the bilaterally and the unilaterally cryptorchid groups, indicating that testicular function was overall decreased in patients with cryptorchidism. Serum AMH was significantly lower in boys with bilateral cryptorchidism as compared with controls and unilaterally cryptorchid patients between 6 months and 1.9 years and between 2 and 8.9 years of age. Serum AMH below the normal range reflected testicular dysfunction in 9.5-36.5% of patients according to the age group in bilaterally cryptorchid boys and 6.3-16.7% in unilaterally cryptorchid boys. FSH was elevated in 8.1% and LH in 9.1% of boys with bilateral cryptorchidism, most of whom were anorchid. In patients with present testes, gonadotropins were only mildly elevated in less than 5% of the cases. Basal testosterone was mildly decreased in patients younger than 6 months old, and uninformative during childhood.
CONCLUSION
Prepubertal boys with cryptorchidism, especially those with bilaterally undescended gonads, have decreased AMH production. Although serum AMH may fall within the normal range, there is a considerable prevalence of testicular dysfunction during childhood in this frequent condition.
PubMed: 29922225
DOI: 10.3389/fendo.2018.00182 -
Acta Stomatologica Croatica Jun 2021Bilateral symmetrical fibrous palate hyperplasia is not a common case in the literature. The cause of this pathological change is not completely known. The development...
Bilateral symmetrical fibrous palate hyperplasia is not a common case in the literature. The cause of this pathological change is not completely known. The development of hyperplasia may also be associated with a genetic mutation in the gingival soft tissue or gingival injury. We present a case of a 47-year-old patient who developed a formation that manifested bilaterally in the hard palate. After the excision, there were no histological elements in the material that indicated aggressive behavior of the formation. The clinical and histological diagnosis was the bilateral symmetrical palate hyperplasia, which is a very rare condition.
PubMed: 34248154
DOI: 10.15644/asc55/2/10 -
Journal of the American Academy of... Aug 2020Bilateral discoid medial menisci are exceptionally rare and of uncertain pathoetiology. We report on a case in an active adolescent woman who presented with bilateral... (Review)
Review
Bilateral discoid medial menisci are exceptionally rare and of uncertain pathoetiology. We report on a case in an active adolescent woman who presented with bilateral medial knee joint line pain. Clinical evaluation and MRI identified atypical bilateral discoid medial menisci bilaterally. The patient underwent bilateral meniscal saucerization, with no pain at 4 months postoperation, and returned to competitive sport.
Topics: Adolescent; Arthroscopy; Female; Humans; Knee Joint; Magnetic Resonance Imaging; Menisci, Tibial; Pain
PubMed: 32769707
DOI: 10.5435/JAAOSGlobal-D-20-00069