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Biomolecules Jan 2021The discovery and development of actinomycete secondary metabolites (ASMs) have played pivotal roles in the fields of human medicine and its related biotechnology...
The discovery and development of actinomycete secondary metabolites (ASMs) have played pivotal roles in the fields of human medicine and its related biotechnology sectors over the past several decades [...].
Topics: Actinobacteria; Actinomyces; Biological Products; Biotechnology; Chemistry, Pharmaceutical; Corynebacterium glutamicum; Drug Discovery; Humans; Multigene Family; Streptomyces
PubMed: 33494267
DOI: 10.3390/biom11020134 -
BMC Biology Aug 2019Cell-free systems (CFS) have recently evolved into key platforms for synthetic biology applications. Many synthetic biology tools have traditionally relied on cell-based... (Review)
Review
Cell-free systems (CFS) have recently evolved into key platforms for synthetic biology applications. Many synthetic biology tools have traditionally relied on cell-based systems, and while their adoption has shown great progress, the constraints inherent to the use of cellular hosts have limited their reach and scope. Cell-free systems, which can be thought of as programmable liquids, have removed many of these complexities and have brought about exciting opportunities for rational design and manipulation of biological systems. Here we review how these simple and accessible enzymatic systems are poised to accelerate the rate of advancement in synthetic biology and, more broadly, biotechnology.
Topics: Biotechnology; Cell-Free System; Synthetic Biology
PubMed: 31395057
DOI: 10.1186/s12915-019-0685-x -
Microbial Biotechnology Sep 2016
Topics: Biotechnology; Industrial Microbiology
PubMed: 27509838
DOI: 10.1111/1751-7915.12403 -
Trends in Biotechnology Mar 2023A series of spectacular scientific discoveries and technological advances in the second half of the 20th century have provided the basis for the ongoing genome editing... (Review)
Review
A series of spectacular scientific discoveries and technological advances in the second half of the 20th century have provided the basis for the ongoing genome editing revolution. The elucidation of structural and functional features of DNA and RNA was followed by pioneering studies on genome editing: Molecular biotechnology was born. Since then, four decades followed during which progress of scientific insights and technological methods continued at an overwhelming pace. Fundamental insights into microbial host-virus interactions led to the development of tools for genome editing using restriction enzymes or the revolutionary CRISPR-Cas technology. In this review, we provide a historical overview of milestones that led to the genome editing revolution and speculate about future trends in biotechnology.
Topics: Gene Editing; CRISPR-Cas Systems; Biotechnology; DNA
PubMed: 36709094
DOI: 10.1016/j.tibtech.2022.12.022 -
Biochemical Society Transactions Feb 2020Since the beginning of the 21st Century, synthetic biology has established itself as an effective technological approach to design and engineer biological systems.... (Review)
Review
Since the beginning of the 21st Century, synthetic biology has established itself as an effective technological approach to design and engineer biological systems. Whilst research and investment continues to develop the understanding, control and engineering infrastructural platforms necessary to tackle ever more challenging systems - and to increase the precision, robustness, speed and affordability of existing solutions - hundreds of start-up companies, predominantly in the US and UK, are already translating learnings and potential applications into commercially viable tools, services and products. Start-ups and SMEs have been the predominant channel for synthetic biology commercialisation to date, facilitating rapid response to changing societal interests and market pull arising from increasing awareness of health and global sustainability issues. Private investment in start-ups across the US and UK is increasing rapidly and now totals over $12bn. Health-related biotechnology applications have dominated the commercialisation of products to date, but significant opportunities for the production of bio-derived materials and chemicals, including consumer products, are now being developed. Synthetic biology start-ups developing tools and services account for between 10% (in the UK) and ∼25% (in the US) of private investment activity. Around 20% of synthetic biology start-ups address industrial biotechnology targets, but currently, only attract ∼11% private investment. Adopting a more networked approach - linking specialists, infrastructure and ongoing research to de-risk the economic challenges of scale-up and supported by an effective long-term funding strategy - is set to transform the impact of synthetic biology and industrial biotechnology in the bioeconomy.
Topics: Biomedical Technology; Biotechnology; Humans; Manufacturing Industry; Synthetic Biology
PubMed: 32077472
DOI: 10.1042/BST20190349 -
Cell Reports Methods Feb 2023We describe an innovative system that exports diverse recombinant proteins in membrane-bound vesicles from . These recombinant vesicles compartmentalize proteins within...
We describe an innovative system that exports diverse recombinant proteins in membrane-bound vesicles from . These recombinant vesicles compartmentalize proteins within a micro-environment that enables production of otherwise challenging insoluble, toxic, or disulfide-bond containing proteins from bacteria. The release of vesicle-packaged proteins supports isolation from the culture and allows long-term storage of active protein. This technology results in high yields of vesicle-packaged, functional proteins for efficient downstream processing for a wide range of applications from discovery science to applied biotechnology and medicine.
Topics: Escherichia coli; Recombinant Proteins; Biotechnology; Escherichia coli Proteins
PubMed: 36936078
DOI: 10.1016/j.crmeth.2023.100396 -
Angewandte Chemie (International Ed. in... Jan 2021Biocatalysis has found numerous applications in various fields as an alternative to chemical catalysis. The use of enzymes in organic synthesis, especially to make... (Review)
Review
Biocatalysis has found numerous applications in various fields as an alternative to chemical catalysis. The use of enzymes in organic synthesis, especially to make chiral compounds for pharmaceuticals as well for the flavors and fragrance industry, are the most prominent examples. In addition, biocatalysts are used on a large scale to make specialty and even bulk chemicals. This review intends to give illustrative examples in this field with a special focus on scalable chemical production using enzymes. It also discusses the opportunities and limitations of enzymatic syntheses using distinct examples and provides an outlook on emerging enzyme classes.
Topics: Biocatalysis; Biotechnology; Enzymes
PubMed: 32558088
DOI: 10.1002/anie.202006648 -
Trends in Biotechnology Feb 2019Microbial consortia have been used in biotechnology processes, including fermentation, waste treatment, and agriculture, for millennia. Today, synthetic biologists are... (Review)
Review
Microbial consortia have been used in biotechnology processes, including fermentation, waste treatment, and agriculture, for millennia. Today, synthetic biologists are increasingly engineering microbial consortia for diverse applications, including the bioproduction of medicines, biofuels, and biomaterials from inexpensive carbon sources. An improved understanding of natural microbial ecosystems, and the development of new tools to construct synthetic consortia and program their behaviors, will vastly expand the functions that can be performed by communities of interacting microorganisms. Here, we review recent advancements in synthetic biology tools and approaches to engineer synthetic microbial consortia, discuss ongoing and emerging efforts to apply consortia for various biotechnological applications, and suggest future applications.
Topics: Biotechnology; Gene Expression Regulation; Metabolic Networks and Pathways; Metabolism; Microbial Consortia; Synthetic Biology
PubMed: 30497870
DOI: 10.1016/j.tibtech.2018.11.002 -
Journal of Microbiology and... Mar 2021Monoclonal antibodies are widely used as diagnostic reagents and for therapeutic purposes, and their demand is increasing extensively. To produce these proteins in... (Review)
Review
Monoclonal antibodies are widely used as diagnostic reagents and for therapeutic purposes, and their demand is increasing extensively. To produce these proteins in sufficient quantities for commercial use, it is necessary to raise the output by scaling up the production processes. This review describes recent trends in high-density cell culture systems established for monoclonal antibody production that are excellent methods to scale up from the lab-scale cell culture. Among the reactors, hollow fiber bioreactors contribute to a major part of high-density cell culture as they can provide a tremendous amount of surface area in a small volume for cell growth. As an alternative to hollow fiber reactors, a novel disposable bioreactor has been developed, which consists of a polymer-based supermacroporous material, cryogel, as a matrix for cell growth. Packed bed systems and disposable wave bioreactors have also been introduced for high cell density culture. These developments in high-density cell culture systems have led to the monoclonal antibody production in an economically favourable manner and made monoclonal antibodies one of the dominant therapeutic and diagnostic proteins in biopharmaceutical industry.
Topics: Animals; Antibodies, Monoclonal; Antibody Formation; Bioreactors; Biotechnology; Cell Culture Techniques; Humans
PubMed: 32238761
DOI: 10.4014/jmb.1911.11066 -
Microbial Biotechnology Jan 2022
Topics: Biotechnology
PubMed: 34767688
DOI: 10.1111/1751-7915.13969