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Journal of Bacteriology Nov 1944
PubMed: 16560867
DOI: 10.1128/jb.48.5.555-557.1944 -
European Journal of Biochemistry Mar 1968
Topics: Animals; Anti-Bacterial Agents; Chemical Phenomena; Chemistry; Hydrogen-Ion Concentration; Indicators and Reagents; Membrane Potentials; Membranes; Methods; Mitochondria, Liver; Oxidation-Reduction; Rats; Spectrophotometry; Time Factors
PubMed: 5646151
DOI: 10.1111/j.1432-1033.1968.tb00166.x -
Food Chemistry Jan 2023Intelligent packaging that provides real-time information on food quality is useful for consumers. We developed a kimchi ripening indicator that can determine the...
Intelligent packaging that provides real-time information on food quality is useful for consumers. We developed a kimchi ripening indicator that can determine the ripeness of kimchi inside packaging and evaluated its applicability and limitations. The indicator was made using calcium hydroxide, which captures CO, and four pH-sensitive dyes (cresol red, bromothymol blue, bromocresol purple, and methyl red). Fourier-transform infrared spectra of the prepared powders showed shapes similar to that of calcium hydroxide, and the dyes were evenly distributed on the calcium hydroxide surfaces. When the developed indicators were evaluated for kimchi packaging application, the indicator made from synthesized calcium hydroxide and bromothymol blue was the most reliable and clearly reflected useful kimchi ripening information. The indicator developed in this study is judged to be practically usable at temperatures of 4-15 °C. However, its usefulness is limited in that the seller cannot change the packaging capacity or kimchi capacity.
Topics: Bromcresol Purple; Bromthymol Blue; Calcium Hydroxide; Carbon Dioxide; Coloring Agents; Fermentation; Fermented Foods; Food Packaging; Hydrogen-Ion Concentration
PubMed: 36055147
DOI: 10.1016/j.foodchem.2022.134039 -
Journal of Clinical Microbiology Nov 1988Bromthymol blue, at a concentration of 0.1% in tryptose-glucose broth, inhibited growth of 98.4% of Escherichia coli serotype O157:H7 isolates but only 0.8% of E. coli...
Bromthymol blue, at a concentration of 0.1% in tryptose-glucose broth, inhibited growth of 98.4% of Escherichia coli serotype O157:H7 isolates but only 0.8% of E. coli non-O157:H7 isolates after an overnight incubation at 44.5 degrees C, but not 35 degrees C. The inhibition was dependent on temperature, density of inoculum, bromthymol blue concentration, time of incubation, and composition of the medium. Compared with serologic typing, the inhibition had sensitivity, specificity, predictive values of the positive and negative tests, and overall agreement between the two tests of 98.4, 99.2, 98.4, 99.2, and 98.9%, respectively. The inhibition could be useful as a presumptive test to identify E. coli isolates of serotype O157:H7, especially in laboratories that do not have serotyping capabilities.
Topics: Bromthymol Blue; Escherichia coli; Humans; Serotyping; Thymol
PubMed: 3069858
DOI: 10.1128/jcm.26.11.2248-2249.1988 -
European Journal of Biochemistry Nov 1968
Topics: Adenosine Triphosphate; Animals; Dinitrophenols; Hydrogen-Ion Concentration; Membrane Potentials; Membranes; Mitochondria, Liver; Osmolar Concentration; Phenolphthaleins; Rats; Rotenone; Spectrophotometry; Succinates
PubMed: 5725505
DOI: 10.1111/j.1432-1033.1968.tb00439.x -
International Journal of Biological... May 2023Continuous wound monitoring is one strategy to minimise infection severity and inform prompt variations in therapeutic care following infection diagnosis. However,...
Continuous wound monitoring is one strategy to minimise infection severity and inform prompt variations in therapeutic care following infection diagnosis. However, integration of this functionality in therapeutic wound dressings is still challenging. We hypothesised that a theranostic dressing could be realised by integrating a collagen-based wound contact layer with previously demonstrated wound healing capability, and a halochromic dye, i.e. bromothymol blue (BTB), undergoing colour change following infection-associated pH changes (pH: 5-6 ➔ >7). Two different BTB integration strategies, i.e. electrospinning and drop-casting, were pursued to introduce long-lasting visual infection detection capability through retention of BTB within the dressing. Both systems had an average BTB loading efficiency of 99 wt% and displayed a colour change within 1 min of contact with simulated wound fluid. Drop-cast samples retained up to 85 wt% of BTB after 96 h in a near-infected wound environment, in contrast to the fibre-bearing prototypes, which released over 80 wt% of BTB over the same time period. An increase in collagen denaturation temperature (DSC) and red shifts (ATR-FTIR) suggest the formation of secondary interactions between the collagen-based hydrogel and the BTB, which are attributed to count for the long-lasting dye confinement and durable dressing colour change. Given the high L929 fibroblast viability in drop-cast sample extracts (92 %, 7 days), the presented multiscale design is simple, cell- and regulatory-friendly, and compliant with industrial scale-up. This design, therefore, offers a new platform for the development of theranostic dressings enabling accelerated wound healing and prompt infection diagnosis.
Topics: Humans; Precision Medicine; Bandages; Collagen; Wound Healing; Surgical Wound Infection; Bromthymol Blue; Hydrogels
PubMed: 36870632
DOI: 10.1016/j.ijbiomac.2023.123866 -
Journal of Clinical Pathology Oct 1976Amniotic fluid lecithin and sphingomyelin areas after extraction and chromatography are rendered permanently visible by the use of bromthymol blue dye buffered to a pH...
Amniotic fluid lecithin and sphingomyelin areas after extraction and chromatography are rendered permanently visible by the use of bromthymol blue dye buffered to a pH of 11-3.
Topics: Amniotic Fluid; Bromthymol Blue; Chromatography, Thin Layer; Female; Humans; Methods; Phosphatidylcholines; Pregnancy; Sphingomyelins
PubMed: 977767
DOI: 10.1136/jcp.29.10.908 -
Journal of Clinical Microbiology Mar 1982A simple new agar medium containing L-canavanine, glycine, and bromthymol blue was found to give a clearer and more accurate distinction between serotype A or D...
A simple new agar medium containing L-canavanine, glycine, and bromthymol blue was found to give a clearer and more accurate distinction between serotype A or D (Cryptococcus neoformans var. neoformans) and serotype B or C (C. neoformans var. gattii) than creatinine-dextrose-bromthymol blue or glycine-cycloheximide-phenol red media. Among 143 isolates of serotype A or D and 70 isolates of serotype B or C, the new medium correlated completely with the serotype, whereas nearly 11% of these isolates gave discrepant reactions with creatinine-dextrose-bromthymol blue and glycine-cycloheximide-phenol red media.
Topics: Cryptococcus; Cryptococcus neoformans; Culture Media; Serotyping
PubMed: 7042750
DOI: 10.1128/jcm.15.3.535-537.1982 -
European Journal of Biochemistry Jan 1969
Topics: Bromine; Chemical Phenomena; Chemistry; Chlorides; Dinitrophenols; Halogens; Hydrogen-Ion Concentration; Ions; Metals; Mitochondria, Muscle; Osmolar Concentration; Phenolphthaleins; Protein Binding; Radioisotopes; Spectrophotometry
PubMed: 5765737
DOI: 10.1111/j.1432-1033.1969.tb19602.x -
A Convenient, Rapid, Sensitive, and Reliable Spectrophotometric Assay for Adenylate Kinase Activity.Molecules (Basel, Switzerland) Feb 2019Enzymatic activity assays are essential and critical for the study of enzyme kinetics. Adenylate kinase (Adk) plays a fundamental role in cellular energy and nucleotide...
Enzymatic activity assays are essential and critical for the study of enzyme kinetics. Adenylate kinase (Adk) plays a fundamental role in cellular energy and nucleotide homeostasis. To date, assays based on different principles have been used for the determination of Adk activity. Here, we show a spectrophotometric analysis technique to determine Adk activity with bromothymol blue as a pH indicator. We analyzed the effects of substrates and the pH indicator on the assay using orthogonal design and then established the most optimal assay for Adk activity. Subsequently, we evaluated the thermostability of Adk and the inhibitory effect of KCl on Adk activity with this assay. Our results show that this assay is simple, rapid, and precise. It shows great potential as an alternative to the conventional Adk activity assay. Our results also suggest that orthogonal design is an effective approach, which is very suitable for the optimization of complex enzyme reaction conditions.
Topics: Adenylate Kinase; Bromthymol Blue; Homeostasis; Hydrogen-Ion Concentration; Kinetics; Phosphorylation; Potassium Chloride; Spectrophotometry
PubMed: 30781833
DOI: 10.3390/molecules24040663