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European Journal of Biochemistry Mar 1968
Topics: Animals; Anti-Bacterial Agents; Chemical Phenomena; Chemistry; Hydrogen-Ion Concentration; Indicators and Reagents; Membrane Potentials; Membranes; Methods; Mitochondria, Liver; Oxidation-Reduction; Rats; Spectrophotometry; Time Factors
PubMed: 5646151
DOI: 10.1111/j.1432-1033.1968.tb00166.x -
Biochimica Et Biophysica Acta Apr 1963
Topics: Bromthymol Blue; Hemoglobins; Humans; Myoglobin; Oxygen; Phenolphthaleins
PubMed: 14013376
DOI: 10.1016/0006-3002(63)90991-0 -
Journal of Bacteriology Nov 1944
PubMed: 16560867
DOI: 10.1128/jb.48.5.555-557.1944 -
European Journal of Biochemistry Jan 1969
Topics: Bromine; Chemical Phenomena; Chemistry; Chlorides; Dinitrophenols; Halogens; Hydrogen-Ion Concentration; Ions; Metals; Mitochondria, Muscle; Osmolar Concentration; Phenolphthaleins; Protein Binding; Radioisotopes; Spectrophotometry
PubMed: 5765737
DOI: 10.1111/j.1432-1033.1969.tb19602.x -
Nature Jul 1964
Topics: Anti-Bacterial Agents; Bacillus cereus; Bromthymol Blue; Coloring Agents; Enterococcus faecalis; Pharmacology; Phenolphthaleins; Polymyxin B; Polymyxins; Proteus; Research; Staphylococcus
PubMed: 14208283
DOI: 10.1038/203211b0 -
Journal of Clinical Pathology Oct 1976Amniotic fluid lecithin and sphingomyelin areas after extraction and chromatography are rendered permanently visible by the use of bromthymol blue dye buffered to a pH...
Amniotic fluid lecithin and sphingomyelin areas after extraction and chromatography are rendered permanently visible by the use of bromthymol blue dye buffered to a pH of 11-3.
Topics: Amniotic Fluid; Bromthymol Blue; Chromatography, Thin Layer; Female; Humans; Methods; Phosphatidylcholines; Pregnancy; Sphingomyelins
PubMed: 977767
DOI: 10.1136/jcp.29.10.908 -
The Journal of Pharmacy and Pharmacology May 1986Rats were immunized intraperitoneally with ovalbumin and the disappearance of bromthymol blue (BTB) from the intestinal lumen, its accumulation in the tissue, and its...
Immunological control of drug absorption from the gastrointestinal tract: the mechanism whereby intestinal anaphylaxis interferes with the intestinal absorption of bromthymol blue in the rat.
Rats were immunized intraperitoneally with ovalbumin and the disappearance of bromthymol blue (BTB) from the intestinal lumen, its accumulation in the tissue, and its net absorption were examined by means of an in-situ recirculation technique during local anaphylaxis. The disappearance of BTB from the intestinal lumen and its net absorption were significantly reduced, but there was no significant effect on its accumulation in the tissue. The pH value of the luminal solution and the perfusate volume were not influenced by intraluminal challenge with the antigen in ovalbumin-immunized rats. In addition, no significant effect was observed on intestinal permeability to BTB in the in-vitro everted sac technique. The intestinal blood flow, measured by a hydrogen clearance method, was not reduced significantly by the intraluminal exposure to antigen. There was enhanced Evans Blue leakage and mucus release in the perfusate after intraluminal challenge with ovalbumin in ovalbumin-immunized rats, but not in non-immunized rats. A significant increase of BTB binding with macromolecular substances in the perfusate was observed during the local anaphylaxis. These findings suggest that the decreased absorption of BTB is due to the interaction with the macromolecular substances in the perfusate during local anaphylaxis.
Topics: Anaphylaxis; Animals; Bromthymol Blue; Digestive System; Evans Blue; Hydrogen-Ion Concentration; Intestinal Absorption; Intestines; Male; Mucus; Ovalbumin; Pharmaceutical Preparations; Rats; Rats, Inbred Strains; Regional Blood Flow; Thymol
PubMed: 2872311
DOI: 10.1111/j.2042-7158.1986.tb04587.x -
Biochemical and Biophysical Research... Oct 1969
Topics: Anti-Bacterial Agents; Bacterial Chromatophores; Buffers; Cresols; Hydrogen-Ion Concentration; Indicators and Reagents; Light; Rhodospirillum
PubMed: 5823935
DOI: 10.1016/0006-291x(69)90728-1 -
Journal of Clinical Microbiology Nov 1988Bromthymol blue, at a concentration of 0.1% in tryptose-glucose broth, inhibited growth of 98.4% of Escherichia coli serotype O157:H7 isolates but only 0.8% of E. coli...
Bromthymol blue, at a concentration of 0.1% in tryptose-glucose broth, inhibited growth of 98.4% of Escherichia coli serotype O157:H7 isolates but only 0.8% of E. coli non-O157:H7 isolates after an overnight incubation at 44.5 degrees C, but not 35 degrees C. The inhibition was dependent on temperature, density of inoculum, bromthymol blue concentration, time of incubation, and composition of the medium. Compared with serologic typing, the inhibition had sensitivity, specificity, predictive values of the positive and negative tests, and overall agreement between the two tests of 98.4, 99.2, 98.4, 99.2, and 98.9%, respectively. The inhibition could be useful as a presumptive test to identify E. coli isolates of serotype O157:H7, especially in laboratories that do not have serotyping capabilities.
Topics: Bromthymol Blue; Escherichia coli; Humans; Serotyping; Thymol
PubMed: 3069858
DOI: 10.1128/jcm.26.11.2248-2249.1988 -
Annals of the New York Academy of... Oct 1969
Topics: Animals; Cresols; Hydrogen-Ion Concentration; In Vitro Techniques; Indicators and Reagents; Membrane Potentials; Membranes; Mitochondria, Liver; Rats
PubMed: 5261240
DOI: 10.1111/j.1749-6632.1969.tb41295.x