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Environmental Science & Technology Apr 2022Peracetic acid has quickly gained ground in water treatment over the last decade. Specifically, its disinfection efficacy toward a wide spectrum of microorganisms in...
Peracetic acid has quickly gained ground in water treatment over the last decade. Specifically, its disinfection efficacy toward a wide spectrum of microorganisms in wastewater is accompanied by the simplicity of its handling and use. Moreover, peracetic acid represents a promising option to achieve disinfection while reducing the concentration of typical chlorination byproducts in the final effluent. However, its chemical behavior is still amply debated. In this study, the reactivity of peracetic acid in the presence of halides, namely, chloride and bromide, was investigated in both synthetic waters and in a real contaminated water. While previous studies focused on the ability of this disinfectant to form halogenated byproducts in the presence of dissolved organic matter and halides, this work indicates that peracetic acid also contributes itself as a primary source in the formation of these potentially carcinogenic compounds. Specifically, this study suggests that 1.5 mM peracetic acid may form around 1-10 μg/L of bromoform when bromide is present. Bromoform formation reaches a maximum at near neutral pH, which is highly relevant for wastewater management.
Topics: Bromides; Disinfectants; Disinfection; Peracetic Acid; Wastewater; Water Pollutants, Chemical; Water Purification
PubMed: 35357818
DOI: 10.1021/acs.est.1c06118 -
BMC Oral Health Jun 2023Disinfection of a 3D-printed surgical guide is of utmost importance as it comes into contact with hard and soft tissue during implant placement so it poses a potential...
BACKGROUND/OBJECTIVE
Disinfection of a 3D-printed surgical guide is of utmost importance as it comes into contact with hard and soft tissue during implant placement so it poses a potential risk of pathogenic transmission. Methods used for disinfection in the surgical field should be reliable, practical, and safe for the instruments and the patients. The objectives of this study were to compare the antimicrobial potential of 100% Virgin Coconut Oil, 2% Glutaraldehyde, and 70% Ethyl Alcohol used to decontaminate 3D-printed surgical guides.
MATERIALS AND METHODS
Thirty identical surgical guides were printed and cut into two halves (N = 60). Both halves were then contaminated with a defined amount of human saliva samples (2 ml). The first half (n = 30) was sub-grouped into three study groups which were immersed in one of the three disinfectants for 20 min as follows; group VCO was immersed in 100% Virgin Coconut Oil, group GA was immersed in 2% Glutaraldehyde, and group EA was immersed in 70% Ethyl Alcohol. The second half (n* = 30) was sub-grouped into three control groups which were immersed in sterile distilled water as follows group VCO*, group GA*, and group EA*. The microbial count was expressed as colony-forming units per plate and the comparison of the antimicrobial potential of the three tested disinfectants between the three study and three control groups was done using the One-Way ANOVA test.
RESULTS
The culture results of three study groups revealed no bacterial growth with the highest % of reduction in the mean microbial count of the oral microorganisms (about100%) and an uncountable bacterial growth was shown between the three control groups (more than 100 CFU/plate) representing the baseline of the oral microorganisms. Therefore; statistically significant differences were found between the three control and three study groups (P < .001).
CONCLUSION
The antimicrobial potential of Virgin Coconut Oil was comparable and equivalent to Glutaraldehyde and Ethyl Alcohol with a significant inhibitory action against oral pathogens.
Topics: Humans; Disinfection; Coconut Oil; Glutaral; Anti-Infective Agents; Ethanol; 2-Propanol; Printing, Three-Dimensional; Disinfectants
PubMed: 37301954
DOI: 10.1186/s12903-023-03092-x -
Journal of Medicine and Life 2019The need to use a natural alternative for disinfecting dental impression materials, which should be biocompatible and effective, led us to evaluate the efficacy of Aloe...
Evaluation of efficacy of Aloe Vera as a Disinfectant by Immersion and Spray methods on Irreversible Hydrocolloid Impression Material and its Effect on the Dimensional Stability of Resultant Gypsum Cast - An in Vitro Study.
The need to use a natural alternative for disinfecting dental impression materials, which should be biocompatible and effective, led us to evaluate the efficacy of Aloe vera as a disinfectant by immersion and spray method on alginate impression material and its effect on the dimensional stability of the resultant gypsum cast. The efficacy of the disinfection procedures against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans was evaluated by determining the number of colony-forming units (CFU) recovered after disinfection of alginate discs inoculated with 1x106 CFU for defined intervals with aloe vera solution (99.96%). Dimensional stability was determined from the mean percentage deviation of three measurements that were taken between the fixed points on the casts using a traveling microscope and compared with corresponding measurements from the master model and controls. Statistical analysis of data was determined by analysis of variance. We found out that there is a mean percentage reduction in colony count of S. aureus, P. aeruginosa, and C. albicans after 3 minutes of immersion in aloe vera and after 3 minutes spray disinfection. Complete elimination of all the microorganism cells after 7 min immersion and spray disinfection. There was a statistically significant difference in the increase of the mean anteroposterior (AP) and cross arch (CA) dimension after 3 and 7 minutes immersion in aloe vera. Spraying with aloe vera for 7 minutes was proved to be the most effective disinfection procedure without altering dimensional stability.
Topics: Alginates; Aloe; Anti-Infective Agents; Bacteria; Calcium Sulfate; Colloids; Colony Count, Microbial; Dental Impression Materials; Disinfectants; Disinfection; Fungi; Microbial Sensitivity Tests
PubMed: 32025258
DOI: 10.25122/jml-2019-0050 -
Frontiers in Public Health 2021Reports of COVID-19 cases potentially attributed to fomite transmission led to the extensive use of various disinfectants to control viral spread. Alternative...
Reports of COVID-19 cases potentially attributed to fomite transmission led to the extensive use of various disinfectants to control viral spread. Alternative disinfectants, such as essential oils, have emerged as a potential antimicrobial. Four essential oil blends were tested on three different surfaces inoculated with a coronavirus surrogate, bacteriophage Phi 6, and a bacterial indicator, . Log concentration reductions were analyzed using GraphPad Prism software. Data collected in this study show that the application of dilute essential oil disinfectants using a spray delivery device is an effective way to reduce concentrations of bacterial and viral microorganisms on ceramic, stainless steel, and laminate surfaces. Surrogate viruses were reduced up to 6 log PFU and bacterial were reduced up to 4 log CFU. Although surfaces are no longer considered a high risk fomite for COVID-19 transmission, the disinfection of microorganisms on surfaces remains an important consideration for high touch areas in hospitals, waiting rooms, etc. The application of spray disinfectants, based on essential oil blends, provides a rapid and effective means to reduce microbial contamination on high-touched surfaces.
Topics: COVID-19; Disinfectants; Disinfection; Humans; Oils, Volatile; SARS-CoV-2
PubMed: 34970529
DOI: 10.3389/fpubh.2021.783832 -
International Journal of Environmental... Jan 2022infections (CDIs) are the most common cause of acquired diseases in hospitalized patients. Effective surface disinfection, focused on the inactivation of the spores of... (Review)
Review
infections (CDIs) are the most common cause of acquired diseases in hospitalized patients. Effective surface disinfection, focused on the inactivation of the spores of this pathogen, is a decisive factor in reducing the number of nosocomial cases of CDI infections. An efficient disinfection procedure is the result of both the properties of the biocidal agent used and the technology of its implementation as well as a reliable, experimental methodology for assessing the activity of the biocidal active substance based on laboratory models that adequately represent real clinical conditions. This study reviews the state of knowledge regarding the properties and biochemical basis of the action mechanisms of sporicidal substances, with emphasis on chlorine dioxide (ClO). Among the analyzed biocides, in addition to ClO, active chlorine, hydrogen peroxide, peracetic acid, and glutaraldehyde were characterized. Due to the relatively high sporicidal effectiveness and effective control of bacterial biofilm, as well as safety in a health and environmental context, the use of ClO is an attractive alternative in the control of nosocomial infections of CD etiology. In terms of the methods of assessing the biocidal effectiveness, suspension and carrier standards are discussed.
Topics: Clostridioides; Clostridioides difficile; Disinfectants; Disinfection; Humans; Spores, Bacterial
PubMed: 35055571
DOI: 10.3390/ijerph19020750 -
Medicina (Kaunas, Lithuania) Sep 2022Background and Objectives: Bacteria and its remnants beneath the restorations predispose the tooth to secondary caries and pulpal pathology. Hence, various chemical... (Randomized Controlled Trial)
Randomized Controlled Trial
Background and Objectives: Bacteria and its remnants beneath the restorations predispose the tooth to secondary caries and pulpal pathology. Hence, various chemical antibacterial agents are suggested to disinfect the prepared tooth structure before the definitive restorative procedure. This study aimed to investigate the effects of chemical disinfectant solutions on the micro-shear bond strength (µSBS) and microhardness of total-etch and self-etch resin-infiltrated human dentin. Materials and Methods: 100 caries-free intact permanent third molar teeth were vertically sectioned into the buccal and lingual half. All these specimens were mounted on acrylic resin and underlying dentin surfaces were exposed by grinding. Samples were randomly divided into five groups [n = 20] following total-etch and self-etch adhesive protocol. Teeth samples were divided according to surface treatment, as Group I (Control-CNT), Group II (2% chlorhexidine-CHX), Group III (5.25% sodium hypochlorite-NaOCl), Group IV (17% ethylenediaminetetraacetate acid—EDTA) and Group V (10% povidone iodine-PVI). A randomly selected 10 samples from each subgroup were used for µSBS and microhardness tests. After surface treatment and bonding procedure, nono-hybrid composite cylinders with a 3-mm diameter and 2-mm height were directly cured over the dentin substrate. The samples for µSBS were subjected to 5000 thermocycles and tested using a universal testing machine. Microhardness was assessed using a micro-indenter instrument, data were statistically analyzed using a one-way analysis of variance and Tukey HSD tests at p < 0.05. Results: Amongst the chemical disinfectant assessed, 2% CHX did not affect µSBS and produced a marginal reduction in dentin microhardness compared to the control group. The 5.25% NaOCl and 17% EDTA significantly compromised the microhardness of the dentin substrate. Meanwhile, 10% PVI surface treatment resulted in a substantial reduction in µSBS between composite and dentin. Conclusions: CHX with preservation of bonding to dentin and insignificant negative effect on dentin microhardness is a safe option for tooth disinfection.
Topics: Acrylic Resins; Adhesives; Anti-Bacterial Agents; Chlorhexidine; Dentin; Dentin-Bonding Agents; Disinfectants; Disinfection; Edetic Acid; Humans; Povidone-Iodine; Resin Cements; Sodium Hypochlorite
PubMed: 36143921
DOI: 10.3390/medicina58091244 -
Water Research Aug 2024Legionella is an opportunistic waterborne pathogen that causes Legionnaires' disease. It poses a significant public health risk, especially to vulnerable populations in...
Legionella is an opportunistic waterborne pathogen that causes Legionnaires' disease. It poses a significant public health risk, especially to vulnerable populations in health care facilities. It is ubiquitous in manufactured water systems and is transmitted via inhalation or aspiration of aerosols/water droplets generated from water fixtures (e.g., showers and hand basins). As such, the effective management of premise plumbing systems (building water systems) in health care facilities is essential for reducing the risk of Legionnaires' disease. Chemical disinfection is a commonly used control method and chlorine-based disinfectants, including chlorine, chloramine, and chlorine dioxide, have been used for over a century. However, the effectiveness of these disinfectants in premise plumbing systems is affected by various interconnected factors that can make it challenging to maintain effective disinfection. This systematic literature review identifies all studies that have examined the factors impacting the efficacy and decay of chlorine-based disinfectant within premise plumbing systems. A total of 117 field and laboratory-based studies were identified and included in this review. A total of 20 studies directly compared the effectiveness of the different chlorine-based disinfectants. The findings from these studies ranked the typical effectiveness as follows: chloramine > chlorine dioxide > chlorine. A total of 26 factors were identified across 117 studies as influencing the efficacy and decay of disinfectants in premise plumbing systems. These factors were sorted into categories of operational factors that are changed by the operation of water devices and fixtures (such as stagnation, temperature, water velocity), evolving factors which are changed in-directly (such as disinfectant concentration, Legionella disinfectant resistance, Legionella growth, season, biofilm and microbe, protozoa, nitrification, total organic carbon(TOC), pH, dissolved oxygen(DO), hardness, ammonia, and sediment and pipe deposit) and stable factors that are not often changed(such as disinfectant type, pipe material, pipe size, pipe age, water recirculating, softener, corrosion inhibitor, automatic sensor tap, building floor, and construction activity). A factor-effect map of each of these factors and whether they have a positive or negative association with disinfection efficacy against Legionella in premise plumbing systems is presented. It was also found that evaluating the effectiveness of chlorine disinfection as a water risk management strategy is further complicated by varying disinfection resistance of Legionella species and the form of Legionella (culturable/viable but non culturable, free living/biofilm associated, intracellular replication within amoeba hosts). Future research is needed that utilises sensors and other approaches to measure these key factors (such as pH, temperature, stagnation, water age and disinfection residual) in real time throughout premise plumbing systems. This information will support the development of improved models to predict disinfection within premise plumbing systems. The findings from this study will inform the use of chlorine-based disinfection within premise plumbing systems to reduce the risk of Legionnaires disease.
Topics: Disinfectants; Chlorine; Legionella; Disinfection; Chlorine Compounds; Water Microbiology; Chloramines; Water Supply; Oxides; Water Purification
PubMed: 38824796
DOI: 10.1016/j.watres.2024.121794 -
Journal of Infection in Developing... Jun 2021Mobile phones are dynamic source of microorganisms in households and professional settings. The aim was to determine the prevalence of bacterial contamination of the...
INTRODUCTION
Mobile phones are dynamic source of microorganisms in households and professional settings. The aim was to determine the prevalence of bacterial contamination of the mobile phones, identify bacterial isolates, assess their antimicrobial susceptibility patterns and define the efficiency of using disinfectant.
METHODOLOGY
This study included 233 dental students from Near East University, Faculty of Dentistry. Swab samples taken from mobile phones before and after disinfection were inoculated onto 5% sheep blood medium and eosin methylene blue medium and incubated aerobically at 37°C for 24-48 hours. Mold-growing mix cultures were sub-cultured on the sabouraud dextrose medium and allowed to grow at room temperature. Conventional microbiological techniques and VITEK 2 automated identification system were used for bacterial identification and antimicrobial susceptibility testing. Antibiotic susceptibility tests were verified by Kirby-Bauer disc diffusion technique according to the European Antimicrobial Susceptibility Test Committee criteria. Mold colonies were identified macroscopic and microscopically according to their phenotypic properties using lacto-phenol cotton blue stain.
RESULTS
Microbial contamination of mobile phones was 81% (120.953 cfu/ml) in swab samples taken without using alcohol-based wipes however, microbial contamination in swab samples taken after one-time disinfection was determined to be 21% (201 cfu/ml). The most common microorganisms isolated were coagulase negative Staphylococci (69%) and Aspergillus niger (13%). All of the isolated bacteria were susceptible to all antibiotics used.
CONCLUSIONS
This study represents the first data on the rate of microbial contamination on mobile phones in Northern Cyprus and the efficiency of the use of alcohol to disinfect the mobile phones.
Topics: Adolescent; Bacteria; Cell Phone; Cyprus; Disinfectants; Disinfection; Equipment Contamination; Female; Humans; Male; Microbial Sensitivity Tests; Students, Dental; Young Adult
PubMed: 34242189
DOI: 10.3855/jidc.13708 -
Biomedical and Environmental Sciences :... Feb 2023To study the effectiveness and feasibility of cryogenic disinfectants in different cold scenarios and analyze the key points of on-site cryogenic disinfection.
OBJECTIVE
To study the effectiveness and feasibility of cryogenic disinfectants in different cold scenarios and analyze the key points of on-site cryogenic disinfection.
METHODS
Qingdao and Suifenhe were selected as application sites for the manual or mechanical spraying of cryogenic disinfectants. The same amount of disinfectant (3,000 mg/L) was applied on cold chain food packaging, cold chain containers, transport vehicles, alpine environments, and article surfaces. The killing log value of the cryogenic disinfectant against the indicator microorganisms ( and ) was used to evaluate the on-site disinfection effect.
RESULTS
When using 3,000 mg/L with an action time of 10 min on the ground in alpine regions, the surface of frozen items, cold-chain containers, and cold chain food packaging in supermarkets, all external surfaces were successfully disinfected, with a pass rate of 100%. The disinfection pass rates for cold chain food packaging and cold chain transport vehicles of centralized supervised warehouses and food processing enterprises were 12.5% (15/120), 81.67% (49/60), and 93.33% (14/15), respectively; yet, the surfaces were not fully sprayed.
CONCLUSION
Cryogenic disinfectants are effective in disinfecting alpine environments and the outer packaging of frozen items. The application of cryogenic disinfectants should be regulated to ensure that they cover all surfaces of the disinfected object, thus ensuring effective cryogenic disinfection.
Topics: Humans; Disinfectants; Disinfection; Escherichia coli; Staphylococcal Infections; Staphylococcus aureus
PubMed: 36861195
DOI: 10.3967/bes2023.019 -
Journal of Occupational and... Aug 2022The list of EPA-approved disinfectants for coronavirus features many products for use on hard, non-porous materials. There are significantly fewer products registered...
The list of EPA-approved disinfectants for coronavirus features many products for use on hard, non-porous materials. There are significantly fewer products registered for use on porous materials. Further, many common, high-touch surfaces fall in between non-porous materials such as glass and porous materials such as soft fabrics. The objective of this study was to assess the efficacy of selected commercially available disinfectant products against coronaviruses on common, high-touch surfaces. Four disinfectants (Clorox Total 360, Bleach solution, Vital Oxide, and Peroxide Multi-Surface Cleaner) were evaluated against Murine Hepatitis Virus A59 (MHV) as a surrogate coronavirus for SARS-CoV-2. MHV in cell culture medium was inoculated onto four materials: stainless steel, latex-painted drywall tape, Styrene Butadiene rubber (rubber), and bus seat fabric. Immediately (T0) or 2-hr (T2) post-inoculation, disinfectants were applied by trigger-pull or electrostatic sprayer and either held for recommended contact times (Spray only) or immediately wiped (Spray and Wipe). Recovered infectious MHV was quantified by median tissue culture infectious dose assay. Bleach solution, Clorox Total 360, and Vital Oxide were all effective (>3-log reduction or complete kill of infectious virus) with both the Spray Only and Spray and Wipe methods on stainless steel, rubber, and painted drywall tape when used at recommended contact times at both T0 and T2 hr. Multi-Surface Cleaner unexpectedly showed limited efficacy against MHV on stainless steel within the recommended contact time; however, it showed increased (2.3 times greater efficacy) when used in the Spray and Wipe method compared to Spray Only. The only products to achieve a 3-log reduction on fabric were Vital Oxide and Clorox Total 360; however, the efficacy of Vital Oxide against MHV on fabric was reduced to below 3-log when applied by an electrostatic sprayer compared to a trigger-pull sprayer. This study highlights the importance of considering the material, product, and application method when developing a disinfection strategy for coronaviruses on high-touch surfaces.
Topics: Animals; COVID-19; Disinfectants; Disinfection; Mice; Murine hepatitis virus; Rubber; SARS-CoV-2; Sodium Hypochlorite; Stainless Steel
PubMed: 35687041
DOI: 10.1080/15459624.2022.2088768