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European Journal of Biochemistry Mar 1976Treatment of phosphorylating fragments of bacterial membrane from Micrococcus lysodeikticus with trypsin leads to increase ATPase activity. As a result of this...
Treatment of phosphorylating fragments of bacterial membrane from Micrococcus lysodeikticus with trypsin leads to increase ATPase activity. As a result of this treatment, the membrane fragments acquire the ability to transform the ATP energy into transmembrane difference in potential. Dithiothreitol has a similar effect to that of trypsin on the membrane fragments from M. lysodeikticus. Dicyclohexylcarbodimide inhibits ATPase of the membrane fragments of M. lysodeikticus, and also the ATPase-reaction-coupled generation of membrane potential. It has been suggested that the increased ATPase activity of membranes from M. lysodeikticus during treatment with trypsin and dithiothreitol is connected with the effect of these agents on the protein inhibitor of ATPase.
Topics: Adenosine Triphosphatases; Cell Membrane; Cyanides; Dicyclohexylcarbodiimide; Hydrogen-Ion Concentration; Malates; Membrane Potentials; Micrococcus; Oxidative Phosphorylation; Oxygen Consumption
PubMed: 4306
DOI: 10.1111/j.1432-1033.1976.tb10196.x -
FEMS Immunology and Medical Microbiology Aug 2010Microorganisms tend to form biofilms on surfaces, thereby causing deterioration of the underlaying material. In addition, biofilm is a potential health risk to humans.... (Comparative Study)
Comparative Study
Microorganisms tend to form biofilms on surfaces, thereby causing deterioration of the underlaying material. In addition, biofilm is a potential health risk to humans. Therefore, microorganism growth is not only an issue on Earth but also in manned space habitats like the International Space Station (ISS). The aim of the study was to identify physiological processes relevant for Micrococcus luteus attachment under microgravity conditions. The results demonstrate that simulated microgravity influences physiological processes which trigger bacterial attachment and biofilm formation. The ISS strains produced larger amounts of exopolymeric substances (EPS) compared with a reference strain from Earth. In contrast, M. luteus strains were growing faster, and Earth as well as ISS isolates produced a higher yield of biomass under microgravity conditions than under normal gravity. Furthermore, microgravity caused a reduction of the colloidal EPS production of ISS isolates in comparison with normal gravity, which probably influences biofilm thickness and stability as well.
Topics: Bacterial Adhesion; Biomass; Biopolymers; Humans; Micrococcus luteus; Stress, Physiological; Weightlessness
PubMed: 20482631
DOI: 10.1111/j.1574-695X.2010.00683.x -
Journal of Bacteriology Dec 1962Field, A. K. (Cornell University, Ithaca, N.Y.) and H. B. Naylor. Induction of lysogenic Micrococcus lysodeikticus. J. Bacteriol. 84:1129-1133. 1962.-Between 50 and 60%...
Field, A. K. (Cornell University, Ithaca, N.Y.) and H. B. Naylor. Induction of lysogenic Micrococcus lysodeikticus. J. Bacteriol. 84:1129-1133. 1962.-Between 50 and 60% of log-phase cells of Micrococcus lysodeikticus strain ML 53-40, lysogenic for N5 bacteriophage, were induced by an optimal dose of ultraviolet light. Induction of dilute cell suspensions caused a subsequent 200- to 800-fold increase in infective-center concentration. Cells in the stationary growth phase and cells adapted to a chemically defined medium were also induced by ultraviolet irradiation. Dimethyl sulfate induced the lysogenic culture to about the same extent as did ultraviolet light, whereas beta-propiolactone was less effective.
Topics: Bacteriophages; Lactones; Lysogeny; Micrococcus; Micrococcus luteus; Sulfates; Ultraviolet Rays
PubMed: 13945051
DOI: 10.1128/jb.84.6.1129-1133.1962 -
Journal of Clinical Microbiology Jan 1995A 4-year prospective study compared the accuracy of the STAPH-IDENT system (bioMérieux Vitek, Inc., Hazelwood, Mo.) with that of the reference procedure of the Centers... (Comparative Study)
Comparative Study
A 4-year prospective study compared the accuracy of the STAPH-IDENT system (bioMérieux Vitek, Inc., Hazelwood, Mo.) with that of the reference procedure of the Centers for Disease Control and Prevention for the identification of Staphylococcus species, Stomatococcus mucilaginosus, and Micrococcus species. The study compared the results from 1,106 cultures (500 eye cultures, 217 strains submitted for reference identification, and 389 known stock strains) representing 21 species of the family Micrococcaceae. The overall agreement of genus and species identifications was 81.1%. The percent agreement for the five most common clinical isolates was as follows: Staphylococcus epidermidis, 97.1% (517 isolates); Staphylococcus hominis, 82.5% (57 isolates); Staphylococcus aureus, 77.2% (162 isolates); Staphylococcus haemolyticus, 75.8% (61 isolates); and Staphylococcus warneri, 64.1% (39 isolates). The lowest percent agreement was with Staphylococcus cohnii (11.1%; (9 isolates). Of the 217 isolates sent to the Centers for Disease Control and Prevention for identification, 60.4% (131) were correctly identified by the STAPH-IDENT system. Of these, S. epidermidis accounted for 23.9%, S. aureus accounted for 15.6%, S. warneri accounted for 6.9%, Staphylococcus lugdunensis accounted for 6.5%, S. haemolyticus accounted for 5.5%, and S. hominis accounted for 4.1%. The STAPH-IDENT system did not perform adequately when dealing with commonly encountered organisms and is unsuitable for identifying uncommon isolates.
Topics: Bacterial Typing Techniques; Centers for Disease Control and Prevention, U.S.; Eye Infections, Bacterial; Humans; Micrococcaceae; Micrococcus; Prospective Studies; Reference Standards; Staphylococcus; United States
PubMed: 7699074
DOI: 10.1128/jcm.33.1.96-98.1995 -
British Medical Journal Jul 1957
Topics: Lung Diseases; Micrococcus; Penicillin V; Penicillins
PubMed: 13446444
DOI: No ID Found -
Canadian Medical Association Journal Sep 1957
Topics: Hospitals; Micrococcus; Staphylococcus
PubMed: 13460883
DOI: No ID Found -
Journal of Bacteriology Nov 1960
Topics: Bacteria; Escherichia coli; Humidity; Micrococcus; Micrococcus luteus
PubMed: 13720225
DOI: 10.1128/jb.80.5.577-579.1960 -
The Biochemical Journal Dec 1962
Topics: Bacteria; Cytoplasm; Escherichia coli; Micrococcus; Micrococcus luteus; Retinoids; Ubiquinone; Vitamin K
PubMed: 13971217
DOI: 10.1042/bj0850550 -
Journal of Clinical Microbiology Jun 1986A rapid, simple lysostaphin lysis susceptibility test to differentiate the genera Staphylococcus and Micrococcus was evaluated. Of 181 strains from culture collections,...
A rapid, simple lysostaphin lysis susceptibility test to differentiate the genera Staphylococcus and Micrococcus was evaluated. Of 181 strains from culture collections, 95 of 95 Staphylococcus strains were lysed, and 79 of 79 Micrococcus strains were not lysed. The seven Planococcus strains were resistant. Clinical isolates (890) were tested with lysostaphin and for the ability to produce acid from glycerol in the presence of erythromycin. Overall agreement between the methods was 99.2%. All clinical Micrococcus strains (43) were resistant to lysostaphin, and all clinical Staphylococcus strains (847) were susceptible. Seven of the Staphylococcus strains did not produce acid from glycerol in the presence of erythromycin. This lysostaphin test provides results in 2 h. It is easier to perform than previously described lysostaphin lysis methods. It is also more rapid and accurate than the glycerol-erythromycin test.
Topics: Bacteriological Techniques; Bacteriolysis; Drug Resistance, Microbial; Erythromycin; Glycerol; Lysostaphin; Micrococcus; Staphylococcus; Staphylococcus aureus; Staphylococcus epidermidis
PubMed: 3519667
DOI: 10.1128/jcm.23.6.1044-1045.1986 -
The Journal of Biological Chemistry Jul 1954
Topics: Acetobacter; Acetoin; Ketones; Micrococcus; Oxidation-Reduction; Pyruvates; Pyruvic Acid
PubMed: 13192087
DOI: No ID Found