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Thorax Feb 1979Clearance rates of soluble radioaerosols of sodium pertechnetate (99mTcO4; mol wt 163) and diethylenetriaminepenta-acetate (99mTc-DTPA; mol wt 492) were determined in...
Clearance rates of soluble radioaerosols of sodium pertechnetate (99mTcO4; mol wt 163) and diethylenetriaminepenta-acetate (99mTc-DTPA; mol wt 492) were determined in seven normal subjects and ten patients with systemic sclerosis affecting the lungs. Twenty millicuries (mCi) each of 99mTcO4 and 99mTc-DTPA in 5 ml saline were aerosolised and inhaled using a disposable "Blount" nebuliser on two different days. Two regions of interest over each posterior lung field were monitored with scintillation camera, and data were stored on magnetic tape using a Hewlett Packard Data Analyser. Decreasing levels of radioactivity were plotted semilogarithmically and half-time (T 1 1/2) removal rates were calculated. The T 1 1/2) values in normal subjects did not differ significantly from T 142 values of the patients with TcO4. However, the removal rates of the higher molecular weight solute were significantly faster from lower lung zones in patients with systemic sclerosis than in the normal subjects. The faster absorption of DTPA from lower lung zones of the patients could be due to regional abnormalities of alveolar epithelium at the lung bases, presumably as a result of greater retractive forces secondary to fibrosis.
Topics: Adult; Aerosols; Female; Humans; Lung; Lung Diseases; Male; Middle Aged; Molecular Weight; Pentetic Acid; Respiratory Function Tests; Scleroderma, Systemic; Technetium
PubMed: 442001
DOI: 10.1136/thx.34.1.63 -
Some quantitative aspects of the labelling of proteins with 125 I by the iodine monochloride method.The Biochemical Journal Jan 1971The labelling of proteins by the iodine monochloride method was studied by using a mathematical model. The equations used were primarily derived from the mass law...
The labelling of proteins by the iodine monochloride method was studied by using a mathematical model. The equations used were primarily derived from the mass law equation of the isotopic exchange reaction between [(125)I]iodide and iodine monochloride. For convenient application, all equations were programmed into a computing desk-top calculator. To support the validity of the theoretical model, a series of iodinations of insulin were performed under various labelling conditions. The results of these experiments compare well with the theoretically derived values. Deviations from the theoretical values occurring at molar ratios of [(125)I]iodide to iodine monochloride < 0.1 and > 4.0 are explained and suggestions made about how to prevent them. The mathematical model was used to simulate the isotopic exchange, and the iodination reaction under various conditions, to study (a) the influence of the amount of [(125)I]iodide on the amount of [(125)I]iodine monochloride formed, (b) the influence of the specific radioactivity of [(125)I]iodide on the amount of [(125)I]iodine monochloride formed, and (c) the influence of the specific radioactivity of [(125)I]iodide on the number of millicuries needed for labelling to a desired extent.
Topics: Chlorides; Insulin; Iodine; Iodine Isotopes; Models, Chemical; Proteins; Radiochemistry
PubMed: 5116527
DOI: 10.1042/bj1210139 -
Journal of Nuclear Medicine : Official... Feb 1991
Topics: Humans; Iodine Radioisotopes; Radiotherapy Dosage; Thyrotoxicosis
PubMed: 1992045
DOI: No ID Found -
Plant Physiology Aug 1986Experiments were designed to test the hypothesis that the labeled products recovered from plant tissue incubated with [(14)C]GA(12)-7-aldehyde ([(14)C]GA(12)ald) would...
Experiments were designed to test the hypothesis that the labeled products recovered from plant tissue incubated with [(14)C]GA(12)-7-aldehyde ([(14)C]GA(12)ald) would serve as appropriate [(14)C]markers for the recovery of naturally-occurring gibberellins (GAs). The [(14)C]GA(12)ald (about 200 millicuries per millimole) was synthesized from pumpkin endosperm using [4,5-(14)C]mevalonic acid. It was added to the adaxial surface of isolated pea cotyledons at 22 days after flowering. Products recovered after 0.5 and 4.0 hour incubations yielded four major peaks which were separated by high performance liquid chromatography (HPLC). These products were purified by multiple-column HPLC using on-line radioactivity detection. They were then added as [(14)C]markers to two unlabeled pea extracts. In general, preparative HPLC followed by further HPLC purification resulted in a single UV-absorbing peak co-eluting with each [(14)C]marker. These [(14)C] and UV-absorbing peaks were shown to contain GA(53), GA(44), GA(20), GA(19), and GA(17) by GC-MS. The finding of GA(53) is novel; all others have previously been found in pea. Endogenous GAs of pea were thus readily detected using [(14)C]GA(12)ald metabolites as [(14)C]markers to recover naturally occurring GAs suggesting that the method may be applicable in detecting naturally occurring GAs in other species.
PubMed: 16664970
DOI: 10.1104/pp.81.4.984 -
Indian Journal of Nuclear Medicine :... Oct 2010Thyroid carcinoma metastatic to the paranasal sinuses is extremely rare. We report a case of follicular thyroid carcinoma metastatic to the right maxillary sinus, with...
Thyroid carcinoma metastatic to the paranasal sinuses is extremely rare. We report a case of follicular thyroid carcinoma metastatic to the right maxillary sinus, with extension into the right side of the hard palate in a young lady. A radioactive iodine-131 (I-131) scan post total thyroidectomy revealed 0.8% uptake in the neck; the whole body scan revealed a functioning metastasis in the region of the right maxillary antrum. Pathological confirmation of metastasis was done by correlating the fine needle aspirate cytology with the thyroidectomy histology. The patient initially received 40 Gy of external beam radiotherapy, subsequently, she received 110 millicuries of I-131, followed by 50 millicuries 6 months later. She continues to be on follow-up on Eltroxin™ suppression and has remained disease free for the past 4 ½ years.
PubMed: 21712913
DOI: 10.4103/0972-3919.78254 -
Journal of Nuclear Medicine : Official... Aug 1991Intra-arterial 99mTc colloid scintigraphy may have greater sensitivity than either standard intravenous scintigraphy or selective arteriography in detecting...
Intra-arterial 99mTc colloid scintigraphy may have greater sensitivity than either standard intravenous scintigraphy or selective arteriography in detecting gastrointestinal bleeding. Ten millicuries of 99mTc colloid were administered directly into the superior and inferior mesenteric arteries (SMA and IMA) of patients who had undergone selective arterial catheterization for the evaluation of gastrointestinal bleeding. In one patient, 99mTc-albumin colloid was administered directly into the IMA and identified diverticular bleeding. The bleeding had been occult to prior contrast arteriography and refractory to selective intra-arterial Pitressin therapy. In a second patient who had undergone three negative provocative angiograms, selective SMA injection of 99mTc-sulfur colloid identified occult mesenteric varices secondary to portal hypertension. Selective intra-arterial scintigraphy should be valuable in detecting intestinal bleeding occult to conventional studies. This will help in directing further therapy and diagnostic evaluation.
Topics: Adult; Aged; Gastrointestinal Hemorrhage; Humans; Injections, Intra-Arterial; Male; Mesenteric Arteries; Radionuclide Angiography; Technetium Tc 99m Aggregated Albumin; Technetium Tc 99m Sulfur Colloid
PubMed: 1869985
DOI: No ID Found