-
Frontiers in Cellular and Infection... 2022The brain development of preterm infants is easily affected by various adverse extrauterine factors and complications, resulting in abnormal neurological and cognitive...
BACKGROUND
The brain development of preterm infants is easily affected by various adverse extrauterine factors and complications, resulting in abnormal neurological and cognitive development. Recent studies have found that there is a significant correlation between intestinal microbial changes and cognitive behavior. Nevertheless, the correlation between the cognitive impairment and abnormal changes of intestinal microflora in the preterm newborn has been rarely elucidated.
AIM
To analyze the differences of fecal intestinal flora, short chain fatty acids (SCFAs) and microbiota-gut-brain axis (MGBA)-related serum factors between preterm birth with and without cognitive impairment.
METHODS
Healthy female rats (body weight 410 ± 40 g) of 16-17 days of gestation were selected for the establishment of preterm cognitive impairment model and screened by Morris water maze navigation experiments. The pathological change of rat hippocampus was confirmed by HE staining. The abundance of fecal intestinal microflora was determined by 16sRNA sequencing, while the contents of fecal SCFAs were examined by gas chromatography.
RESULTS
Compared with the control group, the cognitive impairment group had decreased abundance and diversity of intestinal microflora and increased abundance of at the level of phylum. While the abundances of , , , and decreased significantly at the level of order, family, and genus, the abundances of , , , and increased significantly. Moreover, the levels of total SCFAs and acetic acid in the disease group were significantly lower. The fecal abundance of acetic acid was positively correlated with that of or , and negatively correlated with that of , and in disease rats. Furthermore, cognitive impairment caused significantly decreased levels of 5-HT, GABA, and BDNF, and increased levels of GR, CRH, IL-6, and TNF-α in rat blood.
CONCLUSION
Alterations in intestinal microflora structure and the abundances of SCFAs contributed substantially to the cognitive impairment in preterm rats, which was associated with significant changes in MGBA-related soluble factors.
Topics: Animals; Cognitive Dysfunction; Fatty Acids, Volatile; Female; Gastrointestinal Microbiome; Humans; Infant, Newborn; Infant, Premature; Premature Birth; Rats
PubMed: 36061856
DOI: 10.3389/fcimb.2022.945851 -
Microbiome Aug 2022Monitoring microbial communities especially focused on pathogens in newly developed wastewater treatment systems is recommended for public health. Thus, we investigated...
BACKGROUND
Monitoring microbial communities especially focused on pathogens in newly developed wastewater treatment systems is recommended for public health. Thus, we investigated the microbial community shift in a pilot-scale microalgal treatment system for piggery wastewater.
RESULTS
Microalgae showed reasonable removal efficiencies for COD and ammonia, resulting in higher transparency of the final effluent. Metagenome and microbial diversity analyses showed that heterotrophic microalgal cultivation barely changed the bacterial community; however, the mixotrophic microalgal cultivation induced a sudden change. In addition, an evaluation of risk groups (RGs) of bacteria showed that raw piggery wastewater included abundant pathogens, and the microalgal treatment of the raw piggery wastewater decreased the RG2 pathogens by 63%. However, co-cultivation of microalgae and the most dominant RG2 pathogen, Oligella, showed no direct effects between them.
CONCLUSIONS
Thus, a microbial interaction network was constructed to elucidate algae-bacteria interrelationships, and the decrease in Oligella was indirectly connected with microalgal growth via Brevundimonas, Sphingopyxis, and Stenotrophomonas. In a validation test, 3 among 4 connecting bacterial strains exhibited inhibition zones against Oligella. Therefore, we showed that microalgal wastewater treatment causes a decrease in RG2 bacteria, which is an indirect impact of microalgae associated with bacteria. Video abstract.
Topics: Biomass; Microalgae; Nitrogen; Wastewater; Water Purification
PubMed: 36045433
DOI: 10.1186/s40168-022-01339-3 -
Revista Chilena de Infectologia :... Feb 2012
Topics: Alcaligenaceae
PubMed: 22552519
DOI: 10.4067/S0716-10182012000100016 -
Journal of Clinical Microbiology Aug 2019
PubMed: 31350376
DOI: 10.1128/JCM.01543-18 -
Animals : An Open Access Journal From... May 2022The study reported here aimed to determine whether correlations can be found between the intestinal segment-related microbiota composition and the different growing...
The study reported here aimed to determine whether correlations can be found between the intestinal segment-related microbiota composition and the different growing intensities of broiler chickens. The bacterial community structures of three intestinal segments (jejunum chymus-JC, jejunum mucosa-JM, caecum chymus-CC) from broiler chickens with low body weight (LBW) and high body weight (HBW) were investigated. Similar to the previous results in most cases, significant differences were found in the bacteriota diversity and composition between the different sampling places. However, fewer body weight (BW)-related differences were detected. In the JM of the HBW birds, the / ratio (B/F) was also higher. At the genus level significant differences were observed between the BW groups in the relative abundance of , mainly in the JC; and , mainly in the JM; and , , and in the CC. These genera and others (e.g., and in the JM; , -, and in the CC) showed a close correlation with BW. The co-occurrence interaction results in the JC revealed a correlation between the genera of (mainly with ) and classes with different patterns in the two BW groups. In the JM of LBW birds, two co-occurring communities were found that were not identifiable in HBW chickens and their members belonged to the families of and . In the frame of the co-occurrence evaluation between the jejunal content and mucosa, the two genera ( and in the JC were found to have a significant positive correlation with other genera of the JM only in LBW chickens.
PubMed: 35625142
DOI: 10.3390/ani12101296 -
Cureus Feb 2023is a commensal bacteria genus of the human urinary tract that rarely precipitates clinical infections. We report the case of an asymptomatic 24-year-old male with a...
is a commensal bacteria genus of the human urinary tract that rarely precipitates clinical infections. We report the case of an asymptomatic 24-year-old male with a medical history of Tourette syndrome and the recent placement of deep brain stimulator leads, which were found to be co-infected with species during hardware implantation. This is the first reported case of a deep brain stimulator infection by , a potentially under-recognized and emerging opportunistic bacteria. We review the previously published cases of extra-genitourinary infections and detail the clinical management of this uncommon pathogen.
PubMed: 36949980
DOI: 10.7759/cureus.35133 -
Journal of Clinical Microbiology Mar 1988We determined phenotypic characteristics, cellular fatty acid composition, and isoprenoid quinone content of representative strains of CDC groups EO-2, M-5, and M-6,...
Cultural and chemical characterization of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis.
We determined phenotypic characteristics, cellular fatty acid composition, and isoprenoid quinone content of representative strains of CDC groups EO-2, M-5, and M-6, Moraxella (Moraxella) species, Oligella urethralis, Acinetobacter species, and Psychrobacter immobilis. All organisms contained ubiquinone with eight isoprene units as the major isoprenolog, but distinct differences were observed in fatty acid composition. Twenty-eight of the original collection of CDC group EO-2 strains were further identified as P. immobilis, EO-2, or EO-3 by distinctive cellular fatty acid profiles, cellular morphology, and pigment production. The cellular fatty acid compositions of M-5 and M-6 were similar but were clearly different from those of other organisms. The genus Acinetobacter was differentiated from other organisms in the study by small amounts of 2-hydroxydodecanoic acid (2-OH-12:0), and P. immobilis was differentiated by small amounts of decanoic acid (10:0) and a branched-chain 17-carbon acid (i-17:0). All Moraxella species were distinguished by small amounts of decanoic acid (10:0) and the absence of i-17:0. M. bovis, M. nonliquefaciens, and some strains of M. lacunata formed a single fatty acid group, while M. osloensis, M. phenylpyruvica, M. atlantae, and other strains of M. lacunata (M. lacunata II) had species-specific fatty acid profiles. O. urethralis differed from Moraxella species by the presence of large amounts (49%) of cis-vaccenic acid (18:1 omega 7c), small amounts (1%) of 3-hydroxyhexadecanoate (3-OH-16:0), and the absence of 10:0 and 3-hydroxydodecanoate (3-OH-12:0). The combined use of chemical data and a small number of conventional tests permitted rapid identification and differentiation of these organisms from each other and from related organisms.
Topics: Acinetobacter; Animals; Chromatography, Gas; Fatty Acids; Gram-Negative Bacteria; Humans; Minicomputers; Moraxella; Software; Transformation, Bacterial
PubMed: 3356788
DOI: 10.1128/jcm.26.3.484-492.1988 -
Antibiotics (Basel, Switzerland) Sep 2023is a Gram-negative bacillus, a member of the family, that had never previously been reported as lethal. Herein, a case of fatal infection caused by in an elderly...
is a Gram-negative bacillus, a member of the family, that had never previously been reported as lethal. Herein, a case of fatal infection caused by in an elderly woman with suspected bladder cancer is reported. The species identification was confirmed through Sanger sequencing of the bacterial 16S rRNA sequence and compared to published sequences for phylogenetic analysis. Initial antibiotic therapy with ceftriaxone and oxacillin was initiated but had to be switched due to resistance. Cefepime in combination with metronidazole was administered, unfortunately failing to prevent the patient's death. Further studies are needed to explore additional factors influencing clinical outcomes in infections.
PubMed: 37760766
DOI: 10.3390/antibiotics12091470 -
Le Infezioni in Medicina 2016Oligella ureolytica is an emerging bacteria rarely implicated as a human pathogen. It is mostly recovered from urinary and respiratory tract specimens as a commensal... (Review)
Review
Oligella ureolytica is an emerging bacteria rarely implicated as a human pathogen. It is mostly recovered from urinary and respiratory tract specimens as a commensal organism, but very seldom from bloodstream infections. It is rarely reported in the literature, probably due to misidentification of the organism or uncertainty of its pathogenicity.
Topics: Aged; Alcaligenaceae; Anti-Bacterial Agents; Aortic Valve Stenosis; Bacteremia; Drug Therapy, Combination; Gram-Negative Bacterial Infections; Humans; Male; Penicillanic Acid; Piperacillin; Risk Factors; Tazobactam; Treatment Outcome
PubMed: 27031899
DOI: No ID Found -
Journal of Infection in Developing... Jun 2014Oligella species are small, Gram-negative, nonsaccharolytic aerobic rods or coccobacilli that are catalase and oxidase-positive, mostly isolated from the urinary tract... (Review)
Review
Oligella species are small, Gram-negative, nonsaccharolytic aerobic rods or coccobacilli that are catalase and oxidase-positive, mostly isolated from the urinary tract and rarely from wounds, bloodstream infections, septic arthritis, or peritonitis.In this article, we report a case of O.ureolytica-related bloodstream infection in a newborn infant and we review the literature for previously reported cases of Oligella infections.
Topics: Alcaligenaceae; Anti-Bacterial Agents; Bacteremia; Developing Countries; Female; Gram-Negative Bacterial Infections; Humans; Infant, Newborn; Microbial Sensitivity Tests; Netilmicin; Turkey
PubMed: 24916881
DOI: 10.3855/jidc.3260