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The Canadian Journal of Infectious... Nov 1996A case of Oligella ureolytica infection of a cervical lymph node is presented and previous cases of oligella infection reported in the literature are reviewed....
A case of Oligella ureolytica infection of a cervical lymph node is presented and previous cases of oligella infection reported in the literature are reviewed. Underlying malignancy and urinary tract obstruction were observed in many of the cases. All patients responded to antimicrobial therapy and, in those cases associated with urinary tract obstruction, surgical relief of the obstruction. The microbiology and clinical features of oligella infections are reviewed.
PubMed: 22514466
DOI: 10.1155/1996/153512 -
BMC Microbiology Jan 2022Otitis media (OM) is a major disease burden in Australian Aboriginal children, contributing to serious long-term health outcomes. We report a pilot analysis of OM in...
BACKGROUND
Otitis media (OM) is a major disease burden in Australian Aboriginal children, contributing to serious long-term health outcomes. We report a pilot analysis of OM in children attending an outreach ear and hearing clinic in a remote south Australian community over a two-year period. Our study focuses on longitudinal relationships between ear canal microbiota characteristics with nasopharyngeal microbiota, and clinical and treatment variables.
RESULTS
Middle ear health status were assessed in 19 children (aged 3 months to 8 years) presenting in remote western South Australia and medical interventions were recorded. Over the two-year study period, chronic suppurative OM was diagnosed at least once in 7 children (37%), acute OM with perforation in 4 children (21%), OM with effusion in 11 children (58%), while only 1 child had no ear disease. Microbiota analysis of 19 children (51 sets of left and right ear canal swabs and nasopharyngeal swabs) revealed a core group of bacterial taxa that included Corynebacterium, Alloiococcus, Staphylococcus, Haemophilus, Turicella, Streptococcus, and Pseudomonas. Within-subject microbiota similarity (between ears) was significantly greater than inter-subject similarity, regardless of differences in ear disease (p = 0.0006). Longitudinal analysis revealed changes in diagnosis to be associated with more pronounced changes in microbiota characteristics, irrespective of time interval. Ear microbiota characteristics differed significantly according to diagnosis (P (perm) = 0.0001). Diagnoses featuring inflammation with tympanic membrane perforation clustering separately to those in which the tympanic membrane was intact, and characterised by increased Proteobacteria, particularly Haemophilus influenzae, Moraxella catarrhalis, and Oligella. While nasopharyngeal microbiota differed significantly in composition to ear microbiota (P (perm) = 0.0001), inter-site similarity was significantly greater in subjects with perforated tympanic membranes, a relationship that was associated with the relative abundance of H. influenzae in ear samples (r = - 0.71, p = 0.0003). Longitudinal changes in ear microbiology reflected changes in clinical signs and treatment.
CONCLUSIONS
Children attending the ear and hearing clinic in a remote Aboriginal community present with a broad spectrum of OM conditions and severities, consistent with other remote Aboriginal communities. Ear microbiota characteristics align with OM diagnosis and change with disease course. Nasopharyngeal microbiota characteristics are consistent with the contribution of acute upper respiratory infection to OM aetiology.
Topics: Australia; Bacteria; Child; Child, Preschool; Ear, Middle; Female; Humans; Infant; Longitudinal Studies; Male; Microbiota; Nasopharynx; Native Hawaiian or Other Pacific Islander; Otitis Media; Pilot Projects; Respiratory Tract Infections; Rural Population
PubMed: 35026986
DOI: 10.1186/s12866-022-02436-x -
Animals : An Open Access Journal From... May 2022The study reported here aimed to determine whether correlations can be found between the intestinal segment-related microbiota composition and the different growing...
The study reported here aimed to determine whether correlations can be found between the intestinal segment-related microbiota composition and the different growing intensities of broiler chickens. The bacterial community structures of three intestinal segments (jejunum chymus-JC, jejunum mucosa-JM, caecum chymus-CC) from broiler chickens with low body weight (LBW) and high body weight (HBW) were investigated. Similar to the previous results in most cases, significant differences were found in the bacteriota diversity and composition between the different sampling places. However, fewer body weight (BW)-related differences were detected. In the JM of the HBW birds, the / ratio (B/F) was also higher. At the genus level significant differences were observed between the BW groups in the relative abundance of , mainly in the JC; and , mainly in the JM; and , , and in the CC. These genera and others (e.g., and in the JM; , -, and in the CC) showed a close correlation with BW. The co-occurrence interaction results in the JC revealed a correlation between the genera of (mainly with ) and classes with different patterns in the two BW groups. In the JM of LBW birds, two co-occurring communities were found that were not identifiable in HBW chickens and their members belonged to the families of and . In the frame of the co-occurrence evaluation between the jejunal content and mucosa, the two genera ( and in the JC were found to have a significant positive correlation with other genera of the JM only in LBW chickens.
PubMed: 35625142
DOI: 10.3390/ani12101296 -
Frontiers in Bioengineering and... 2021The effects of banana resistant starch (BRS) on obesity-related metabolic and intestinal flora were investigated in a high-fat diet-induced obesity model. After 6 weeks...
The effects of banana resistant starch (BRS) on obesity-related metabolic and intestinal flora were investigated in a high-fat diet-induced obesity model. After 6 weeks of intervention, the glucolipid metabolism index [blood glucose (GLU), total cholesterol (TC), triacylglycerol (TG), low density lipoprotein-cholesterol (LDL-C), and high density lipoprotein-cholesterol (HDL-C)], hormone index [leptin (LEP), insulin (INS), ghrelin, adiponectin (ADP), and thyroxine (T4)], and 16S rRNA sequencing analyses were performed for each group to explore the regulating effect of intestinal flora and the mechanism of weight loss in obese rats. The results showed that (1) BRS intervention significantly reduced the levels of GLU, TG, TC, LDL-C, LEP, and INS ( < 0.01) and increased the contents of ghrelin ( < 0.05) and ADP ( < 0.01). (2) BRS could improve the diversity of intestinal flora and regulate the overall structure of intestinal microorganisms, mainly by upregulating the / ratio and the relative abundance of and downregulating the relative abundances of and (at the phylum level). BRS could inhibit the proliferation of , , and and increase the abundances of , , and (at the genus level). (3) Some significant correlations were observed between the gut microbiota and biomarkers. , , and were positively correlated with GLU, TG, TC, LEP, and INS and negatively correlated with ghrelin and ADP. , , and were negatively correlated with GLU, TG, and TC. Conclusion: BRS had promising effects on weight loss, which could be associated with the improvement in host metabolism by regulating intestinal flora.
PubMed: 33585429
DOI: 10.3389/fbioe.2021.575724 -
Microbiology Resource Announcements Feb 2024are opportunistic pathogens typically associated with genitourinary infections. Here, we report the complete genome for an isolate recovered from ear discharge of a...
are opportunistic pathogens typically associated with genitourinary infections. Here, we report the complete genome for an isolate recovered from ear discharge of a child with chronic suppurative otitis media (strain MSHR-50412PR). The genome comprises 2.58 Mb, with 2,448 coding sequences and 46.26% average GC content.
PubMed: 38275301
DOI: 10.1128/mra.01071-23 -
Acta Pharmaceutica Sinica. B Feb 2020The progression of hyperuricemia disease is often accompanied by damage to renal function. However, there are few studies on hyperuricemia nephropathy, especially its...
The progression of hyperuricemia disease is often accompanied by damage to renal function. However, there are few studies on hyperuricemia nephropathy, especially its association with intestinal flora. This study combines metabolomics and gut microbiota diversity analysis to explore metabolic changes using a rat model as well as the changes in intestinal flora composition. The results showed that amino acid metabolism was disturbed with serine, glutamate and glutamine being downregulated whilst glycine, hydroxyproline and alanine being upregulated. The combined glycine, serine and glutamate could predict hyperuricemia nephropathy with an area under the curve of 1.00. Imbalanced intestinal flora was also observed. , , , , and other conditional pathogens increased significantly in the model group, while and , the short-chain fatty acid producing bacteria, declined greatly. At phylum, family and genus levels, disordered nitrogen circulation in gut microbiota was detected. In the model group, the uric acid decomposition pathway was enhanced with reinforced urea liver-intestine circulation. The results implied that the intestinal flora play a vital role in the pathogenesis of hyperuricemia nephropathy. Hence, modulation of gut microbiota or targeting at metabolic enzymes, , urease, could assist the treatment and prevention of this disease.
PubMed: 32082971
DOI: 10.1016/j.apsb.2019.10.007 -
Journal of Clinical Microbiology Aug 2019
Topics: Alcaligenaceae; Fatal Outcome; Gram-Negative Bacterial Infections; Humans; Lung Abscess; Lung Neoplasms; Male; Middle Aged; Thorax; Tomography, X-Ray Computed
PubMed: 31350375
DOI: 10.1128/JCM.01542-18 -
GMS Hygiene and Infection Control 2019To quantify the frequency of bacterial contamination of the injected contrast agent/saline solution by an automated contrast injection system, and to evaluate whether...
To quantify the frequency of bacterial contamination of the injected contrast agent/saline solution by an automated contrast injection system, and to evaluate whether usage of a novel tube system can reduce it. For bacterial contamination quantification two identical automated piston pump MRI contrast injectors were used in combination with a standard tube system. 3-5 ml of the contrast agent/saline solution was collected from the system prior to its connection to the patients' venous cannula in 104 consecutive patients. To test, whether a novel tube system reduces contamination, a tube system with shielded screw connections was used with the same contrast injectors and contrast agent/saline samples were collected in further 101 patients. Specimens were microbiologically analyzed. Frequencies of contamination were compared using Fisher exact test. With the standard tube system, bacterial contamination was observed in 5.8% (6 out of 104 specimens). With the novel tube system, contamination was observed in 2.0% (2 out of 101 specimens, p=0.280). was the most common germ (5 cases) followed by (2 cases) and (1 case). Bacterial contaminations of MRI contrast injectors occurred in a non-negligible frequency especially with . A trend towards reduced bacterial contamination was seen when a novel tube system with shielded screw connections was used.
PubMed: 31198659
DOI: 10.3205/dgkh000321 -
Frontiers in Microbiology 2024The rumen microbial community plays a crucial role in the digestion and metabolic processes of ruminants. Although sequencing-based studies have helped reveal the...
INTRODUCTION
The rumen microbial community plays a crucial role in the digestion and metabolic processes of ruminants. Although sequencing-based studies have helped reveal the diversity and functions of bacteria in the rumen, their physiological and biochemical characteristics, as well as their dynamic regulation along the digestion process in the rumen, remain poorly understood. Addressing these gaps requires pure culture studies to demystify the intricate mechanisms at play. Bacteria exhibit morphological differentiation associated with different species. Based on the difference in size or shape of microorganisms, size fractionation by filters with various pore sizes can be used to separate them.
METHODS
In this study, we used polyvinylidene difluoride filters with pore sizes of 300, 120, 80, 40, 20, 8, 6, 2.1, and 0.6 μm. Bacterial suspensions were successively passed through these filters for the analysis of microbial population distribution using 16S rRNA gene sequences.
RESULTS
We found that bacteria from the different pore sizes were clustered into four branches (> 120 μm, 40-120 μm, 6-20 μm, 20-40 μm, and < 0.6 μm), indicating that size fractionation had effects on enriching specific groups but could not effectively separate dominant groups by cell size alone. The species of unclassified Flavobacterium, unclassified Chryseobacterium, unclassified , , unclassified Caulobacteraceae, unclassified , unclassified , unclassified , unclassified , unclassified , unclassified Alphaproteobacteria, and unclassified SR1 can be efficiently enriched or separated by size fractionation.
DISCUSSION
In this study, we investigated the diversity of sorted bacteria populations in the rumen for preliminary investigations of the relationship between the size and classification of rumen bacteria that have the potential to improve our ability to isolate and culture bacteria from the rumen in the future.
PubMed: 38628864
DOI: 10.3389/fmicb.2024.1376994 -
Antimicrobial Agents and Chemotherapy May 2003Clinical Oligella urethralis isolate COH-1, which was uncommonly resistant to penicillins and narrow-spectrum cephalosporins, was recovered from a 55-year-old patient...
Clinical Oligella urethralis isolate COH-1, which was uncommonly resistant to penicillins and narrow-spectrum cephalosporins, was recovered from a 55-year-old patient with a urinary tract infection. Shotgun cloning into Escherichia coli and expression experiments gave recombinant clones expressing either an AmpC beta-lactamase-type phenotype of resistance or a carbenicillin-hydrolyzing beta-lactamase-type phenotype of resistance. The AmpC beta-lactamase identified (ABA-1), which had a pI value of 8.2, had 98% amino acid identity with a chromosomally encoded cephalosporinase of Acinetobacter baumannii. A 820-bp insertion sequence element, ISOur1, belonging to the IS6 family of insertion sequence elements, was identified immediately upstream of bla(ABA-1), providing a -35 promoter sequence and likely giving rise to a hybrid promoter region. The carbenicillin-hydrolyzing beta-lactamase identified (CARB-8), which had a pI value of 6.4, differed from CARB-5 by two amino acid substitutions. Hybridization of CeuI fragment I-restricted DNA fragments of O. urethralis COH-1 with bla(ABA-1)-, bla(CARB-8)-, and 16S rRNA-specific probes indicated the chromosomal integration of the beta-lactamase genes. PCR and hybridization experiments failed to detect bla(CARB-8)- and bla(ABA-1)-like genes in three O. urethralis reference strains, indicating that the beta-lactamase genes identified were the source of acquired resistance in O. urethralis COH-1. This is one of the few examples of the interspecies transfer and the chromosomal integration of a gene encoding a naturally occurring beta-lactamase.
Topics: Acinetobacter baumannii; Amino Acid Sequence; Anti-Bacterial Agents; Bacterial Proteins; Chromosomes, Bacterial; Cloning, Molecular; Conjugation, Genetic; Drug Resistance, Bacterial; Gram-Negative Aerobic Bacteria; Humans; Microbial Sensitivity Tests; Middle Aged; Molecular Sequence Data; beta-Lactamases; beta-Lactams
PubMed: 12709319
DOI: 10.1128/AAC.47.5.1536-1542.2003