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Environmental Microbiome Apr 2022Collembola are soil-dwelling arthropods that play a key role in the soil ecosystem. Allonychiurus kimi (Lee) (Collembola: Onychiuridae) was isolated from the natural...
Collembola are soil-dwelling arthropods that play a key role in the soil ecosystem. Allonychiurus kimi (Lee) (Collembola: Onychiuridae) was isolated from the natural environment and has been maintained for 20 years under laboratory conditions. Though the morphological and physiological features of A. kimi are being widely used to evaluate the impact of pesticides and heavy metals on the soil ecosystem, variations observed in these features might be on account of its microbiota. However, the microbiota composition of the laboratory-maintained A. kimi is undetermined and how the community structure is changing in response to soil environments or interacting with the soil microbiota are still unknown. In this study, we determined the microbiota of laboratory-maintained A. kimi at both adult and juvenile stages and examined how the microbiota of A. kimi is affected by the microbial community in the soil environments. Chryseobacterium, Pandoraea, Sphingomonas, Escherichia-Shigella, and Acinetobacter were the core microbiota of A. kimi. Exposure of the laboratory-maintained A. kimi to different soil microbial communities drove dynamic shifts in the composition of A. kimi microbiota. Microbial association network analysis suggested that gut microbiota of lab-grown A. kimi was affected by exposing to soil microbial community. This study implies that shifts in the bacterial community of adult A. kimi can be utilized as an indicator to evaluate the soil ecosystem.
PubMed: 35382887
DOI: 10.1186/s40793-022-00411-7 -
Infectious Disease Reports Mar 2022is a Gram-negative bacterium of the genus and is mainly associated with the colonization of structurally abnormal airways. During the COVID-19 pandemic, many...
is a Gram-negative bacterium of the genus and is mainly associated with the colonization of structurally abnormal airways. During the COVID-19 pandemic, many microorganisms have been associated with coinfection and superinfection in SARS-CoV-2 pneumonia, but so far, no coinfection or superinfection by has been reported. We present the first case describing this association in a previously healthy patient. Clinical manifestations, treatment, and outcomes are shown.
PubMed: 35314655
DOI: 10.3390/idr14020025 -
Glycoconjugate Journal Apr 2021Pandoraea sp. is an emerging Gram-negative pathogen in cystic fibrosis causing severe and persistent inflammation and damage of the lungs. The molecular mechanisms...
Pandoraea sp. is an emerging Gram-negative pathogen in cystic fibrosis causing severe and persistent inflammation and damage of the lungs. The molecular mechanisms underlying the high pathogenicity of Pandoraea species are still largely unknown. As Gram-negatives, Pandoraea sp. express lipopolysaccharides (LPS) whose recognition by the host immune system triggers an inflammatory response aimed at the bacterial eradication from the infected tissues. The degree of the inflammatory response strongly relies on the fine structure of the LPS and, in particular, of its glycolipid moiety, i.e. the lipid A. Here we report the structure of the lipid A isolated from the LPS of a chronic strain of P. pulmonicola (RL 8228), one of the most virulent identified so far among the Pandoraea species. Our data demonstrated that the examined chronic strain produces a smooth-type LPS with a complex mixture of hypoacylated lipid A species displaying, among other uncommon characteristics, the 2-hydroxylation of some of the acyl chains and the substitution by an additional glucosamine on one or both the phosphate groups.
Topics: Acylation; Burkholderiaceae; Cystic Fibrosis; Humans; Lipid A; Lipopolysaccharides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PubMed: 33048281
DOI: 10.1007/s10719-020-09954-8 -
Acta Medica Portuguesa Feb 2023Pandoraea species are a newly described genus of multidrug-resistant, non-fermentative Gram-negative bacilli, mainly isolated from sputum samples of cystic fibrosis...
Pandoraea species are a newly described genus of multidrug-resistant, non-fermentative Gram-negative bacilli, mainly isolated from sputum samples of cystic fibrosis patients. In immunocompromised patients or with high antibiotic selective pressure, these pathogens are generally opportunistic and invasive. Although Pandoraea spp. are rare, the true incidence of these infections may be underestimated due to difficulties in microbial identification by phenotypic methods. We present the case of a 51-year-old woman, with new-onset fever after a prolonged hospitalization and multiple courses of antibiotics. Mass spectrometry assays identified Pandoraea pnomenusa in the blood cultures taken from the central venous catheter and in the catheter tip. Fever cessation after catheter removal suggests a catheter-related bloodstream infection. To the best of our knowledge, this is the first isolation of a Pandoraea spp. in Portugal, which should raise awareness to the emergence of these opportunistic and multidrug-resistant microorganisms, and the importance of its prompt identification.
Topics: Female; Humans; Middle Aged; Central Venous Catheters; Burkholderiaceae; Anti-Bacterial Agents; Cystic Fibrosis; Catheter-Related Infections
PubMed: 35604812
DOI: 10.20344/amp.16176 -
Revista Chilena de Infectologia :... Oct 2007
Topics: Anti-Bacterial Agents; Burkholderiaceae; Microbial Sensitivity Tests
PubMed: 17989848
DOI: 10.4067/s0716-10182007000500011 -
Microorganisms Aug 2022Microorganisms in extreme volcanic environments play an important role in the development of plants on newly exposed substrates. In this work, we studied the structure...
Microorganisms in extreme volcanic environments play an important role in the development of plants on newly exposed substrates. In this work, we studied the structure and diversity of a bacterial community associated to and at El Chichón volcano. The genetic diversity of the strains was revealed by genomic fingerprints and by 16S rDNA gene sequencing. Furthermore, a metagenomic analysis of the rhizosphere samples was carried out for pioneer plants growing inside and outside the volcano. Multifunctional biochemical tests and plant inoculation assays were evaluated to determine their potential as plant growth-promoting bacteria (PGPB). Through metagenomic analysis, a total of 33 bacterial phyla were identified from and rhizosphere samples collected inside the volcano, and outside the volcano 23 bacterial phyla were identified. For both rhizosphere samples, proteobacteria was the most abundant phylum. With a cultivable approach, 174 bacterial strains were isolated from the rhizosphere and tissue of plants growing outside the volcanic complex. Isolates were classified within the genera and . The evaluated strains were able to produce indole compounds, solubilize phosphate, synthesize siderophores, showed ACC deaminase and nitrogenase activity, and they had a positive effect on the growth and development of . The wide diversity of bacteria associated to pioneer plants at El Chichón volcano with PGPB qualities represent an alternative for the recovery of eroded environments, and they can be used efficiently as biofertilizers for agricultural crops growing under adverse conditions.
PubMed: 36013987
DOI: 10.3390/microorganisms10081568 -
Infection and Drug Resistance 2021Bacteremia by spp. has rarely been described before. We report the first case of a possible prosthetic valve endocarditis, according to the modified Duke criteria, in...
Bacteremia by spp. has rarely been described before. We report the first case of a possible prosthetic valve endocarditis, according to the modified Duke criteria, in a 37-year old male injecting drug user suffering from recurrent endocarditis. Furthermore, we demonstrate biofilm formation by the isolates of this patient and investigate antibiotic resistance.
PubMed: 33854344
DOI: 10.2147/IDR.S301138 -
Microbiology Resource Announcements Dec 2023We report here seven draft genomes of bacterial strains from two Danish wastewater facilities, two of which might be characterized as a new group within the and...
We report here seven draft genomes of bacterial strains from two Danish wastewater facilities, two of which might be characterized as a new group within the and genera, respectively. These genomes will provide useful references for understanding bacterial interactions and horizontal gene transfer within bacterial communities.
PubMed: 37966239
DOI: 10.1128/MRA.00529-23 -
Frontiers in Microbiology 2021The parasitoid fungus , whose fruiting bodies are known in China as "chan hua," literally "cicada flower," has been used as a traditional Chinese medicinal ingredient...
The parasitoid fungus , whose fruiting bodies are known in China as "chan hua," literally "cicada flower," has been used as a traditional Chinese medicinal ingredient for centuries. However, systematic disclosure of the vital factors responsible for the formation of wild cicada flower is limited. Here, we determined the physicochemical properties of soil and simultaneously analyzed the diversities and the structures of microbial community inhabiting the coremia, sclerotia, and soil around wild cicada flowers through high-throughput sequencing. Our results indicated that cicada flower more preferentially occurred in acidic soil (pH 5.9) with abundant moisture content (MC), total nitrogen (TN), and organic matter (OM). The dominant fungal genera in soil mainly included , f__Clavariaceae_Unclassified, , f__Chaetomiaceae_Unclassified, , f__Sordariaceae_Unclassified, and . Among them, was the only fungus that was massively detected in both the coremia and sclerotia with abundance of 83.5 and 53.6%, respectively. Based on this, a strain named AH10-4 with excellent adenosine- and -(2-hydroxyethyl)-adenosine (HEA)-producing capability was successfully isolated. However, to the aspect of bacteria, , , , f__Xanthobacteraceae_Unclassified, and were the dominant genera in soil. , f__Enterobacteriaceae_Unclassified, , , , , and were the dominant genera in the coremia and sclerotia. Notably, was the shared bacteria among them with high abundance of 3.1, 11.4, and 5.2% in the sclerotia, coremia, and soil, respectively. However, the possible role of these bacteria to the occurrence of cicada flower has been unclear to our knowledge. By analyzing the correlation between physicochemical properties and microbial community of soil, we found that MC, Fe, and Zn were significantly negatively correlated with soil and that Cu was significantly negatively correlated with most dominant soil bacterial genera. But Mg was significantly positively correlated with most dominant taxa. This study provides new insight into the formation mechanisms of cicada flower and may contribute to the large-scale cultivation of cicada flowers.
PubMed: 34899639
DOI: 10.3389/fmicb.2021.752791 -
Scientific Reports Jul 2019Temperature, which is an important environmental factor in broiler farming, can significantly influence the deposition of fatty acids in muscle. 300 one-day-old broiler...
Temperature, which is an important environmental factor in broiler farming, can significantly influence the deposition of fatty acids in muscle. 300 one-day-old broiler chicks were randomly divided into three groups and reared at high, medium and low temperatures (HJ, MJ and LJ), respectively. Breast muscle and jejunal chyme samples were collected and subjected to analyses of fatty acid composition and 16S rRNA gene sequencing. Through spearman's rank correlation coefficient, the data were used to characterize the correlation between jejunal microbial diversity and muscle fatty acid deposition in the broilers. The results showed that Achromobacter, Stenotrophomonas, Pandoraea, Brevundimonas, Petrobacter and Variovorax were significantly enriched in the MJ group, and all of them were positively correlated with the fatty acid profiling of muscle and multiple lipid metabolism signaling pathways. Lactobacillus was significantly enriched in the HJ group and exhibited a positive correlation with fatty acid deposition. Pyramidobacter, Dialister, Bacteroides and Selenomonas were significantly enriched in the LJ group and displayed negative correlation with fatty acid deposition. Taken together, this study demonstrated that the jejunal microflora manifested considerable changes at high and low ambient temperatures and that jejunal microbiota changes were correlated with fatty acid deposition of muscle in broilers.
Topics: Animals; Chickens; Fatty Acids; Gastrointestinal Microbiome; Jejunum; Metagenome; Muscle, Skeletal; Temperature
PubMed: 31363155
DOI: 10.1038/s41598-019-47323-0