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Current Developments in Nutrition Jan 2021Anemia is a major cause of morbidity and mortality of pregnant women and increases the risks of fetal and neonatal morbidity and mortality. Approximately 50% of all...
Anemia is a major cause of morbidity and mortality of pregnant women and increases the risks of fetal and neonatal morbidity and mortality. Approximately 50% of all anemia is estimated to be caused by low dietary intake of iron, poor absorption of dietary iron, or blood loss. The objective of the present study was to determine the prevalence of and assess the dietary habits associated with anemia in pregnant women receiving antenatal care (ANC) in Unguja Island, Tanzania. A cross-sectional study was conducted to select 338 pregnant women at Kivunge, Mwembeladu, and Mnazimmoja hospitals from March to June 2018. Hemoglobin concentration was measured using a HemoCue photometer on capillary blood. Sociodemographic data and dietary habits were collected using a structured questionnaire. Multivariate logistic regression analysis was carried out to determine the predictors of anemia in pregnant women. The overall prevalence of anemia was 80.8%. Of these 68.64% had mild anemia, 11.24% had moderate anemia, and 0.89% had severe anemia. Anemia was significantly associated with inadequate dietary diversity [adjusted OR (AOR): 1.16; 95% CI: 0.57, 2.36; < 0.05], drinking tea or coffee with a meal (AOR: 0.06; 95% CI: 0.03, 0.13; < 0.001), consuming <3 meals/d (AOR: 2.92; 95% CI: 1.60, 5.84; < 0.001), higher education level (AOR: 3.4; 95% CI: 1.6, 7.2; < 0.0001), birth interval <2 y (AOR: 3.6; 95% CI: 1.1, 11.9; < 0.05), and multigravida status (AOR: 1.2; 95% CI: 0.3, 4.4; < 0.0001). The prevalence of anemia in this study demonstrates a severe public health problem among pregnant women. Inadequate dietary diversity coupled with inadequate daily meal intake and consumption of tea or coffee were the dietary habits predicting anemia in pregnant women. Other predictors of anemia were higher education level, multigravida status, and birth interval <2 y. Nutrition policy interventions are needed to complement ANC services by providing important information on healthy eating habits during pregnancy.
PubMed: 33501404
DOI: 10.1093/cdn/nzaa178 -
Proceedings of the National Academy of... Feb 2018Capabilities for recording neural activity in behaving mammals have greatly expanded our understanding of brain function. Some of the most sophisticated approaches use...
Capabilities for recording neural activity in behaving mammals have greatly expanded our understanding of brain function. Some of the most sophisticated approaches use light delivered by an implanted fiber-optic cable to optically excite genetically encoded calcium indicators and to record the resulting changes in fluorescence. Physical constraints induced by the cables and the bulk, size, and weight of the associated fixtures complicate studies on natural behaviors, including social interactions and movements in environments that include obstacles, housings, and other complex features. Here, we introduce a wireless, injectable fluorescence photometer that integrates a miniaturized light source and a photodetector on a flexible, needle-shaped polymer support, suitable for injection into the deep brain at sites of interest. The ultrathin geometry and compliant mechanics of these probes allow minimally invasive implantation and stable chronic operation. In vivo studies in freely moving animals demonstrate that this technology allows high-fidelity recording of calcium fluorescence in the deep brain, with measurement characteristics that match or exceed those associated with fiber photometry systems. The resulting capabilities in optical recordings of neuronal dynamics in untethered, freely moving animals have potential for widespread applications in neuroscience research.
Topics: Animals; Brain; Deep Brain Stimulation; Male; Mice; Mice, Inbred C57BL; Neurons; Optical Fibers; Optogenetics; Photic Stimulation; Wireless Technology
PubMed: 29378934
DOI: 10.1073/pnas.1718721115 -
IEEE Transactions on Industry... Nov 2019Lighting regulations for luminance in U. S. coal mines are verified in the field by using a luminance photometer calibrated to the Standard Illuminant A light source....
Lighting regulations for luminance in U. S. coal mines are verified in the field by using a luminance photometer calibrated to the Standard Illuminant A light source. Significant measurement errors can exist when measuring light sources that are dissimilar to light sources used to calibrate the photometer. This paper quantifies the measurement errors when measuring these dissimilar light sources commonly used in U.S. underground coal mines-an LED, a CFL with a clear cover, a CFL with an amber cover, and a tungsten halogen. The impact of photometer quality was also evaluated. Three different luminance measuring instruments of high, medium, and low quality were compared-a PR-650, LS-100, and PMEX, respectively. The PMEX was under evaluation for measuring luminance compliance in U.S. underground coal mines. The PR-650 was used as the referent to which the other photometers were compared. The PMEX error ranged from -17.0% to -26.5% with the highest error for the amber CFL. The LS-100 closely matched the luminance measurement for the LED and halogen; however, it had a percent error of -10.4% for the amber CFL. After the initial experiment, MSHA made improvements to the PMEX resulting in the PMEX-MSHA. The experiment was replicated using the new photometer and the newer PR-670. After repeating the experiment, the measurement errors ranged from -16% to -19% for the PMEX-MSHA, thus indicating an improvement over the PMEX. These results show that the spectral content of a light source and the photometer quality can greatly impact the accuracy of luminance measurement.
PubMed: 31768079
DOI: 10.1109/TIA.2019.2933808 -
Journal of Applied Clinical Medical... Dec 2019In this work, we evaluated the change of primary monitor characteristics in two consecutive years. Sixty-six primary monitors were included in the analysis. The monitors...
In this work, we evaluated the change of primary monitor characteristics in two consecutive years. Sixty-six primary monitors were included in the analysis. The monitors were located at radiology physicians' offices and radiology reading rooms. All primary monitors were equipped with the manufacturer's built-in photometers and connected to the BarcoMediCalQA web service for manual and automatic quality control measurements. External photometer/illuminance meter (RaySafe Solo Light) was used to measure the luminance values. Measured luminance values of the TG18LN1-18 and TG18UNL80 test patterns were used to evaluate the primary monitors performance. In a comparison of the quality assurance (QA) measurement results for the same monitors that were performed within 2 years, the luminance of 25 displays remained statistically the same (P > 0.01). The luminance of 17 displays decreased (P < 0.01) in 2017 when compared with 2016, the luminance of 24 displays increased (P < 0.01) in 2017 when compared with 2016. For the annual measurements of the MLD in 2016 and 2017, 25 out of 66 displays showed a decrease of MLD values in 2017 compared with the same measurements in 2016 and 41 displays showed an increase of MLD in 2017. All tested primary displays had the MLD value less than 17.2%. The mean value of illuminance measured in 2016 was 5.8 lux ± 3.1 lux. In 2017, the mean value of illuminance measured was 8.7 lux ± 5.3 lux. Although it is expected that monitors luminance values will decrease over time, we found displays with increased luminance. This is possibly due to the multiple monitor calibrations that were performed between two annual monitor QA tests. Based on the findings of this work, more efficient display QA programs with a shorter time interval than 1 year are needed.
Topics: Calibration; Computer Graphics; Data Display; Diagnostic Imaging; Humans; Luminescent Measurements; Photometry; Quality Control; Radiology Information Systems; Time Factors
PubMed: 31833641
DOI: 10.1002/acm2.12759 -
Advanced Biomedical Research 2017Nontuberculous mycobacteria (NTM) are a group of opportunistic pathogens and these are widely dispersed in water and soil resources. Identification of mycobacteria...
BACKGROUND
Nontuberculous mycobacteria (NTM) are a group of opportunistic pathogens and these are widely dispersed in water and soil resources. Identification of mycobacteria isolates by conventional methods including biochemical tests, growth rates, colony pigmentation, and presence of acid-fast bacilli is widely used, but these methods are time-consuming, labor-intensive, and may sometimes remain inconclusive.
MATERIALS AND METHODS
The DNA was extracted from NTM cultures using CTAB, Chelex, Chelex + Nonidet P-40, FTA Elute card, and boiling The quantity and quality of the DNA extracted via these methods were determined using UV-photometer at 260 and 280 nm, and polymerase chain reaction (PCR) amplification of the heat-shock protein 65 gene with serially diluted DNA samples.
RESULTS
The CTAB method showed more positive results at 1:10-1:100,000 at which the DNA amount was substantial. With the Chelex method of DNA extraction, PCR amplification was detected at 1:10 and 1:1000 dilutions.
CONCLUSIONS
According to the electrophoresis results, the CTAB and Chelex DNA extraction methods were more successful in comparison with the others as regard producing suitable concentrations of DNA with the minimum use of PCR inhibitor.
PubMed: 29279831
DOI: 10.4103/2277-9175.217216 -
Brazilian Journal of Biology = Revista... 2022Honey is one of the best nutritious substances in the world, having different services in the body functions regulation. Ten elements (K, Na, Ca, Co, Cr, Mn, Mo, Ni, Pb,...
Honey is one of the best nutritious substances in the world, having different services in the body functions regulation. Ten elements (K, Na, Ca, Co, Cr, Mn, Mo, Ni, Pb, Se) from honey samples were analyzed from 80 different locations of Punjab and ten floras. The aim of the present study was to determine the quality and quantity of minerals and Physico-chemical analysis in honey. A flame photometer was used to measure the concentration of major minerals (K, Ca and Na). The concentration of micro minerals (Co, Cr, Mn, Mo, Ni, Pb and Se) was analyzed using Atomic Absorption Spectrometer. The concentration of macro-elements obtained was as follow (in ppm): K (166-1732), Na (107-418) and Ca (07-99), while the concentration of microelements (in ppm) Co (1-2), Cr (>1), Mn (<1), Mo (1.818), Ni (1.911), Pb (<1) and Se (1.968). The most abundant minerals were potassium, calcium and sodium, ranging between 396-810.5, 17.5-640.63 and 169.88-238.62 ppm, respectively. However, the trace mineral elements of honey were obtained in the order of decreasing Se > Co > Ni > Pb > Cr > Mo > Mn. The findings showed that all the heavy metals like Co, Cr, Ni and Pb were present in trace amounts and close to International Honey Quality Standard. The result of given honey samples represented highest value of moisture (31.23%), color (80 mm pfund), pH (8.23), acidity (72.02 meq/kg), electrical conductivity (0.85 ms/cm) and ash contents (0.83%).
Topics: Animals; Bees; Honey; Lead; Metals, Heavy; Minerals; Trace Elements
PubMed: 35857954
DOI: 10.1590/1519-6984.263534 -
Animals : An Open Access Journal From... May 2023Artificial insemination in the swine industry, as in other species, demands adequate semen handling and accurate evaluation for the preparation of seminal doses. Sperm...
Artificial insemination in the swine industry, as in other species, demands adequate semen handling and accurate evaluation for the preparation of seminal doses. Sperm concentration and motility estimates are part of the semen evaluation process and are considered important for maximizing the yield of doses for insemination. In this study, methods were examined for their accuracy in the estimation of boar sperm concentration and motility. Assessments of sperm concentration were carried out using iSperm, ISAS v1, Open CASA v2, and the Accuread photometer. Analyses of sperm motility were performed with iSperm, ISAS v1, and Open CASA v2 systems. In this study, boar semen samples were collected from 10 healthy males from two genetic lines. There were no relevant differences between sire lines when sperm concentration was assessed. A Bayesian analysis was applied to the four methods used to assess sperm concentration to examine whether there are relevant differences between them. Results suggested differences in the four methods, with a probability of relevance (PR) of 0.86-1.00. The iSperm method revealed higher concentration values within the highest posterior density region at 95% confidence interval (HPD) = 167.0, 224.2 M/mL, whereas Open CASA v2 showed the lowest values, with HPD = 99.3, 155.9 M/mL. The iSperm demonstrated higher reliability in measuring sperm concentration compared to other methods or devices within the given range of confidence. ANOVAs revealed relevant differences in the three methods of motility estimation. Overall, differences in boar sperm concentration and motility estimates were found using various methods, but further studies are needed for better characterization of these differences.
PubMed: 37238052
DOI: 10.3390/ani13101622 -
Pharmaceutics Jul 2023Medicated chewing gum with lysozyme hydrochloride and ascorbic acid as active pharmaceutical ingredients was developed for application in dentistry. The aim of this...
The Study of the Cytotoxicity, Proliferative and Microbiological Activity of the Medicated Chewing Gum with Ascorbic Acid and Lysozyme Hydrochloride Using Different Culture of Cells.
Medicated chewing gum with lysozyme hydrochloride and ascorbic acid as active pharmaceutical ingredients was developed for application in dentistry. The aim of this research was to study the cytotoxicity, proliferative, and microbiological activities of the active ingredients in different types of cell cultures. The preclinical study of active pharmaceutical ingredients and their combinations was carried out using culture lines such as HepG2 (human hepatocarcinoma cells), Hek293 (human embryonic kidney cells), and MAEC (mouse aortic endothelial cells). MTT assays were used to analyse cytotoxicity and proliferative activity, while the state of antioxidant protection was assessed by the content of sulfhydryl groups and catalase activity. The determination of lipid peroxidation products was based on the level of TBA-active products. As a microbiological model for studying the effect of the developed dental medicine on the ability of the oral cavity microorganisms to form biofilms, the following strains were used: , , , , and . The optical density of the formed biofilm was evaluated by the intensity of the experimental sample's colour on a StatFax 303 Plus photometer at a wavelength of 630 nm. The combination of ascorbic acid and lysozyme hydrochloride in the established concentrations (20 mg and 10 mg per 1 gum, respectively) resulted in a slight stimulation of cell proliferation without any toxic effects and increased antioxidant protection, preventing the development of oxidative stress. It was found that, in contrast to the separately used active substances, the combination of lysozyme hydrochloride and ascorbic acid inhibits the biofilm formation of all studied microorganisms and shows the ability to destroy diurnal biofilms of and fungi of the genus , indicating potentiation and summation of the active pharmaceutical ingredients' composition effects in the developed dental medicine. Due to the observed positive pharmacological and microbiological action, the combination of lysozyme hydrochloride and ascorbic acid in the medicated chewing gum serves as a promising tool for the prevention and treatment of infectious and inflammatory diseases of the periodontium and mucous membranes and the prevention of caries.
PubMed: 37514080
DOI: 10.3390/pharmaceutics15071894 -
Data in Brief Oct 2023This dataset highlights some of the water quality issues in Uganda. The rationale for collecting the water samples was to test and ascertain the level and source of...
This dataset highlights some of the water quality issues in Uganda. The rationale for collecting the water samples was to test and ascertain the level and source of contamination. A total of one hundred and eighty five samples were collected from sixteen districts. At each water point, a sample was collected using a sterile plastic container, which was pre-rinsed with the water to be sampled. Water samples were drawn from protected and unprotected springs, shallow wells, taps, rain tanks, water reservoirs, open and hand dug wells and boreholes and immediately transported on ice to the National Water Quality Reference Laboratory for analysis. At the laboratory, a BWB flame photometer, Ethylenediamine tetraacetic acid (EDTA) titration and gallery plus-thermos fisher discreet analyzer were used to analyze metal, nutrient and anion elements. On-site testing of dissolved oxygen, pH, electrical conductivity and turbidity was done using a water data sonde. This data can be used to draw comparative analyses of water quality issues in rural and urban districts and help in identifying the factors that influence water quality variations. The data can further be used for trend analysis and identifying long-term patterns whilst providing insights into pollution sources and the impact of environmental and climate change. Consequently, mathematical and machine learning models can use this data together with other parameters to predict the changes in water quality which information is essential for policy and decisions making. This data can be used by environmental scientists to draw insights into the health of the aquatic biodiversity; geospatial analysts to ascertain proximal water contaminants; public health specialists to analyze pathogens leading to water-borne diseases; water chemists to study the source and cause of water pollution; data scientists to perform predictive and descriptive analyses; and policy makers to formulate laws and regulations.
PubMed: 37808544
DOI: 10.1016/j.dib.2023.109601 -
Medycyna Pracy Dec 2023The study aimed to evaluate the effectiveness of filtering facepiece respirators (FFRs) in reducing odour nuisances in agricultural work environment. Additionally, an...
BACKGROUND
The study aimed to evaluate the effectiveness of filtering facepiece respirators (FFRs) in reducing odour nuisances in agricultural work environment. Additionally, an assessment was conducted on the microbiological contamination of FFRs and the functionality of Time4Mask application in enhancing workplace safety.
MATERIAL AND METHODS
Two types of FFRs were used for the study: with absorbing properties and reference ones. The research was carried out in 6 livestock rooms during a 1-week period in early spring (February-March 2021) on a farm in central Poland. The microclimate conditions (thermoanemometer), and particulate matter concentrations (laser photometer) were assessed. Additionally, the odour content in the studied rooms and the breathing zone of FFR users (gas chromatography with mass spectrometry) were evaluated. The number of microorganisms on the respirators was determined (cultivation method), followed by their identification (biochemical tests, taxonomic keys). Breakthrough curves were determined for both FFR types to assess absorption capabilities.
RESULTS
The average temperature in the livestock rooms was about 13°C, relative humidity - 53%, air flow velocity - 0.21 m/s, and particulate matter concentration - 0.216 mg/m. A significant variety of odorants was found in the environment and the breathing zone under the FFRs. Bacterial counts ranged between 2.4 × 10 and 2.6 × 10 CFU/cm, fungi between 3.2 × 10 and 5.4 × 10 CFU/cm, xerophilic fungi from 4.4 × 10 to 4.0 × 10 CFU/cm, mannitol-positive staphylococci between 1.6 × 10 and 1.0 × 10 CFU/cm, and haemolytic staphylococci from 2.2 × 10 to 4.5 × 10 CFU/cm, depending on the respirator type. Respirators were colonized by bacteria from the genera: , , actinobacteria sp., and fungi: , , , , and . Respirators with absorbing properties had over 8-times longer breakthrough time than reference ones.
CONCLUSIONS
Respirators with activated carbon effectively improved work comfort when exposed to odours. Due to growth of microorganisms in the respirator materials, periodic replacement is necessary. It is crucial to provide workers with information about the safe-use time of respirators, considering environmental conditions. This is achievable using modern IT tools like Time4Mask application. Med Pr Work Health Saf. 2023;76(5):363-75.
Topics: Humans; Air Pollutants, Occupational; Occupational Exposure; Odorants; Gas Chromatography-Mass Spectrometry; Particulate Matter; Ventilators, Mechanical; Fungi; Bacteria; Respiratory Protective Devices
PubMed: 38104337
DOI: 10.13075/mp.5893.01441